Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS17470 and LRK53_RS17475 are separated by 195 nucleotides LRK53_RS17475 and LRK53_RS17480 are separated by 63 nucleotides LRK53_RS17480 and LRK53_RS17485 are separated by 68 nucleotides LRK53_RS17485 and LRK53_RS17490 are separated by 24 nucleotides LRK53_RS17490 and LRK53_RS17495 are separated by 25 nucleotides
LRK53_RS17470: LRK53_RS17470 - nicotinamidase, at 3,834,052 to 3,834,702
_RS17470
LRK53_RS17475: LRK53_RS17475 - CPXCG motif-containing cysteine-rich protein, at 3,834,898 to 3,835,086
_RS17475
LRK53_RS17480: LRK53_RS17480 - putative toxin-antitoxin system toxin component, PIN family, at 3,835,150 to 3,835,608
_RS17480
LRK53_RS17485: LRK53_RS17485 - hypothetical protein, at 3,835,677 to 3,835,931
_RS17485
LRK53_RS17490: LRK53_RS17490 - HAD family hydrolase, at 3,835,956 to 3,836,678
_RS17490
LRK53_RS17495: LRK53_RS17495 - hypothetical protein, at 3,836,704 to 3,837,225
_RS17495
Position (kb)
3835
3836 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3834.693 kb on + strand at 3834.694 kb on - strand at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.001 kb on + strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.002 kb on - strand, within LRK53_RS17475 at 3835.055 kb on + strand, within LRK53_RS17475 at 3835.055 kb on + strand, within LRK53_RS17475 at 3835.056 kb on - strand, within LRK53_RS17475 at 3835.115 kb on + strand at 3835.115 kb on + strand at 3835.115 kb on + strand at 3835.115 kb on + strand at 3835.116 kb on - strand at 3835.116 kb on - strand at 3835.116 kb on - strand at 3835.131 kb on - strand at 3835.161 kb on - strand at 3835.433 kb on + strand, within LRK53_RS17480 at 3835.640 kb on + strand at 3835.640 kb on + strand at 3835.640 kb on + strand at 3835.640 kb on + strand at 3835.641 kb on - strand at 3835.641 kb on - strand at 3835.641 kb on - strand at 3835.701 kb on + strand at 3835.701 kb on + strand at 3835.701 kb on + strand at 3835.701 kb on + strand at 3835.701 kb on + strand at 3835.702 kb on - strand at 3835.702 kb on - strand at 3835.740 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.788 kb on + strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.789 kb on - strand, within LRK53_RS17485 at 3835.791 kb on + strand, within LRK53_RS17485 at 3835.791 kb on + strand, within LRK53_RS17485 at 3835.791 kb on + strand, within LRK53_RS17485 at 3835.791 kb on + strand, within LRK53_RS17485 at 3835.792 kb on - strand, within LRK53_RS17485 at 3835.911 kb on + strand at 3835.911 kb on + strand at 3835.911 kb on + strand at 3835.911 kb on + strand at 3835.911 kb on + strand at 3835.912 kb on - strand at 3835.912 kb on - strand at 3835.912 kb on - strand at 3835.912 kb on - strand at 3835.912 kb on - strand at 3835.912 kb on - strand at 3835.958 kb on - strand at 3836.090 kb on - strand, within LRK53_RS17490 at 3836.209 kb on + strand, within LRK53_RS17490 at 3836.210 kb on - strand, within LRK53_RS17490 at 3836.210 kb on - strand, within LRK53_RS17490 at 3836.213 kb on + strand, within LRK53_RS17490 at 3836.214 kb on - strand, within LRK53_RS17490 at 3836.214 kb on - strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.329 kb on + strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.330 kb on - strand, within LRK53_RS17490 at 3836.363 kb on + strand, within LRK53_RS17490 at 3836.441 kb on + strand, within LRK53_RS17490 at 3836.441 kb on + strand, within LRK53_RS17490 at 3836.441 kb on + strand, within LRK53_RS17490 at 3836.441 kb on + strand, within LRK53_RS17490 at 3836.441 kb on + strand, within LRK53_RS17490 at 3836.441 kb on + strand, within LRK53_RS17490 at 3836.441 kb on + strand, within LRK53_RS17490 at 3836.442 kb on - strand, within LRK53_RS17490 at 3836.442 kb on - strand, within LRK53_RS17490 at 3836.442 kb on - strand, within LRK53_RS17490 at 3836.504 kb on - strand, within LRK53_RS17490 at 3836.578 kb on + strand, within LRK53_RS17490 at 3836.578 kb on + strand, within LRK53_RS17490 at 3836.579 kb on - strand, within LRK53_RS17490 at 3836.621 kb on - strand at 3836.621 kb on - strand at 3836.623 kb on + strand at 3836.623 kb on + strand at 3836.623 kb on + strand at 3836.623 kb on + strand at 3836.623 kb on + strand at 3836.624 kb on - strand at 3836.624 kb on - strand at 3836.624 kb on - strand at 3836.624 kb on - strand at 3836.624 kb on - strand at 3836.624 kb on - strand at 3836.801 kb on + strand, within LRK53_RS17495 at 3836.801 kb on + strand, within LRK53_RS17495 at 3836.802 kb on - strand, within LRK53_RS17495 at 3836.802 kb on - strand, within LRK53_RS17495 at 3836.838 kb on - strand, within LRK53_RS17495 at 3836.861 kb on + strand, within LRK53_RS17495 at 3836.862 kb on - strand, within LRK53_RS17495 at 3836.862 kb on - strand, within LRK53_RS17495 at 3836.903 kb on + strand, within LRK53_RS17495 at 3836.903 kb on + strand, within LRK53_RS17495 at 3836.904 kb on - strand, within LRK53_RS17495 at 3836.904 kb on - strand, within LRK53_RS17495 at 3836.904 kb on - strand, within LRK53_RS17495
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 3,834,693 + +1.4 3,834,694 - +0.6 3,835,001 + LRK53_RS17475 0.54 -1.1 3,835,001 + LRK53_RS17475 0.54 -0.9 3,835,001 + LRK53_RS17475 0.54 +1.4 3,835,001 + LRK53_RS17475 0.54 +0.6 3,835,001 + LRK53_RS17475 0.54 -0.2 3,835,001 + LRK53_RS17475 0.54 -1.2 3,835,001 + LRK53_RS17475 0.54 -0.7 3,835,001 + LRK53_RS17475 0.54 -1.8 3,835,001 + LRK53_RS17475 0.54 -0.9 3,835,001 + LRK53_RS17475 0.54 +0.1 3,835,001 + LRK53_RS17475 0.54 +2.4 3,835,001 + LRK53_RS17475 0.54 -0.9 3,835,001 + LRK53_RS17475 0.54 -1.9 3,835,001 + LRK53_RS17475 0.54 -2.4 3,835,001 + LRK53_RS17475 0.54 +1.4 3,835,001 + LRK53_RS17475 0.54 -0.5 3,835,001 + LRK53_RS17475 0.54 +2.4 3,835,001 + LRK53_RS17475 0.54 +1.4 3,835,001 + LRK53_RS17475 0.54 -0.9 3,835,001 + LRK53_RS17475 0.54 -0.9 3,835,001 + LRK53_RS17475 0.54 +0.8 3,835,002 - LRK53_RS17475 0.55 +1.4 3,835,002 - LRK53_RS17475 0.55 +0.4 3,835,002 - LRK53_RS17475 0.55 -1.4 3,835,002 - LRK53_RS17475 0.55 +0.4 3,835,002 - LRK53_RS17475 0.55 +0.0 3,835,002 - LRK53_RS17475 0.55 +1.4 3,835,002 - LRK53_RS17475 0.55 -0.2 3,835,002 - LRK53_RS17475 0.55 -1.2 3,835,002 - LRK53_RS17475 0.55 -0.2 3,835,002 - LRK53_RS17475 0.55 -3.9 3,835,055 + LRK53_RS17475 0.83 +2.4 3,835,055 + LRK53_RS17475 0.83 -2.4 3,835,056 - LRK53_RS17475 0.84 +0.4 3,835,115 + -0.7 3,835,115 + +0.4 3,835,115 + -0.6 3,835,115 + -1.2 3,835,116 - +0.4 3,835,116 - -0.7 3,835,116 - -0.9 3,835,131 - -0.5 3,835,161 - -0.6 3,835,433 + LRK53_RS17480 0.62 +0.4 3,835,640 + -0.2 3,835,640 + +0.4 3,835,640 + -2.2 3,835,640 + -0.4 3,835,641 - +0.0 3,835,641 - +0.4 3,835,641 - +0.8 3,835,701 + -0.2 3,835,701 + +0.7 3,835,701 + -0.2 3,835,701 + +1.2 3,835,701 + +2.0 3,835,702 - -0.6 3,835,702 - +1.4 3,835,740 + LRK53_RS17485 0.25 +1.4 3,835,788 + LRK53_RS17485 0.44 -0.9 3,835,788 + LRK53_RS17485 0.44 +1.9 3,835,788 + LRK53_RS17485 0.44 +0.4 3,835,788 + LRK53_RS17485 0.44 -0.2 3,835,788 + LRK53_RS17485 0.44 -1.4 3,835,788 + LRK53_RS17485 0.44 -1.8 3,835,788 + LRK53_RS17485 0.44 -0.6 3,835,788 + LRK53_RS17485 0.44 +2.0 3,835,788 + LRK53_RS17485 0.44 -1.4 3,835,788 + LRK53_RS17485 0.44 -1.4 3,835,789 - LRK53_RS17485 0.44 -0.1 3,835,789 - LRK53_RS17485 0.44 -2.6 3,835,789 - LRK53_RS17485 0.44 -0.3 3,835,789 - LRK53_RS17485 0.44 -0.2 3,835,789 - LRK53_RS17485 0.44 -0.2 3,835,789 - LRK53_RS17485 0.44 +0.4 3,835,789 - LRK53_RS17485 0.44 -0.4 3,835,789 - LRK53_RS17485 0.44 -1.7 3,835,789 - LRK53_RS17485 0.44 -2.0 3,835,789 - LRK53_RS17485 0.44 -1.3 3,835,789 - LRK53_RS17485 0.44 -0.4 3,835,789 - LRK53_RS17485 0.44 -2.6 3,835,791 + LRK53_RS17485 0.45 -3.8 3,835,791 + LRK53_RS17485 0.45 -1.8 3,835,791 + LRK53_RS17485 0.45 -0.8 3,835,791 + LRK53_RS17485 0.45 -0.2 3,835,792 - LRK53_RS17485 0.45 +0.1 3,835,911 + -2.0 3,835,911 + +0.4 3,835,911 + -0.2 3,835,911 + -0.6 3,835,911 + -2.4 3,835,912 - -1.9 3,835,912 - +1.4 3,835,912 - -1.2 3,835,912 - -0.2 3,835,912 - -1.5 3,835,912 - -3.1 3,835,958 - -0.6 3,836,090 - LRK53_RS17490 0.19 -0.9 3,836,209 + LRK53_RS17490 0.35 -1.9 3,836,210 - LRK53_RS17490 0.35 -0.6 3,836,210 - LRK53_RS17490 0.35 +0.8 3,836,213 + LRK53_RS17490 0.36 -0.2 3,836,214 - LRK53_RS17490 0.36 -2.8 3,836,214 - LRK53_RS17490 0.36 +0.3 3,836,329 + LRK53_RS17490 0.52 -0.2 3,836,329 + LRK53_RS17490 0.52 -0.0 3,836,329 + LRK53_RS17490 0.52 +0.4 3,836,329 + LRK53_RS17490 0.52 -0.9 3,836,329 + LRK53_RS17490 0.52 +1.2 3,836,329 + LRK53_RS17490 0.52 +0.1 3,836,329 + LRK53_RS17490 0.52 -1.2 3,836,329 + LRK53_RS17490 0.52 -0.8 3,836,329 + LRK53_RS17490 0.52 -1.4 3,836,329 + LRK53_RS17490 0.52 -0.2 3,836,329 + LRK53_RS17490 0.52 +1.4 3,836,329 + LRK53_RS17490 0.52 -0.9 3,836,330 - LRK53_RS17490 0.52 +0.6 3,836,330 - LRK53_RS17490 0.52 +0.4 3,836,330 - LRK53_RS17490 0.52 -1.4 3,836,330 - LRK53_RS17490 0.52 +1.4 3,836,330 - LRK53_RS17490 0.52 +0.8 3,836,330 - LRK53_RS17490 0.52 -0.4 3,836,330 - LRK53_RS17490 0.52 +1.4 3,836,330 - LRK53_RS17490 0.52 -0.2 3,836,330 - LRK53_RS17490 0.52 +0.4 3,836,330 - LRK53_RS17490 0.52 -0.6 3,836,330 - LRK53_RS17490 0.52 +1.4 3,836,330 - LRK53_RS17490 0.52 +0.4 3,836,363 + LRK53_RS17490 0.56 +1.4 3,836,441 + LRK53_RS17490 0.67 -1.4 3,836,441 + LRK53_RS17490 0.67 -0.6 3,836,441 + LRK53_RS17490 0.67 +1.0 3,836,441 + LRK53_RS17490 0.67 +0.7 3,836,441 + LRK53_RS17490 0.67 -0.9 3,836,441 + LRK53_RS17490 0.67 +0.2 3,836,441 + LRK53_RS17490 0.67 -1.2 3,836,442 - LRK53_RS17490 0.67 +2.4 3,836,442 - LRK53_RS17490 0.67 +0.4 3,836,442 - LRK53_RS17490 0.67 +2.0 3,836,504 - LRK53_RS17490 0.76 +0.7 3,836,578 + LRK53_RS17490 0.86 +1.4 3,836,578 + LRK53_RS17490 0.86 +0.8 3,836,579 - LRK53_RS17490 0.86 -0.2 3,836,621 - +1.0 3,836,621 - -0.9 3,836,623 + -1.2 3,836,623 + -1.1 3,836,623 + -1.0 3,836,623 + -0.3 3,836,623 + +0.8 3,836,624 - +1.4 3,836,624 - +2.4 3,836,624 - -0.9 3,836,624 - -1.9 3,836,624 - -0.4 3,836,624 - +0.4 3,836,801 + LRK53_RS17495 0.19 -0.9 3,836,801 + LRK53_RS17495 0.19 -1.2 3,836,802 - LRK53_RS17495 0.19 +0.0 3,836,802 - LRK53_RS17495 0.19 -2.0 3,836,838 - LRK53_RS17495 0.26 +1.4 3,836,861 + LRK53_RS17495 0.30 +0.5 3,836,862 - LRK53_RS17495 0.30 -0.2 3,836,862 - LRK53_RS17495 0.30 +0.8 3,836,903 + LRK53_RS17495 0.38 -1.2 3,836,903 + LRK53_RS17495 0.38 -1.7 3,836,904 - LRK53_RS17495 0.38 -0.4 3,836,904 - LRK53_RS17495 0.38 +0.6 3,836,904 - LRK53_RS17495 0.38 +0.2
Or see this region's nucleotide sequence