Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16325 and LRK53_RS16330 are separated by 80 nucleotides LRK53_RS16330 and LRK53_RS16335 are separated by 26 nucleotides LRK53_RS16335 and LRK53_RS16340 are separated by 60 nucleotides LRK53_RS16340 and LRK53_RS16345 are separated by 19 nucleotides
LRK53_RS16325: LRK53_RS16325 - nucleoside permease, at 3,594,403 to 3,595,683
_RS16325
LRK53_RS16330: LRK53_RS16330 - TonB-dependent receptor plug domain-containing protein, at 3,595,764 to 3,596,378
_RS16330
LRK53_RS16335: LRK53_RS16335 - PepSY domain-containing protein, at 3,596,405 to 3,597,487
_RS16335
LRK53_RS16340: LRK53_RS16340 - CDP-alcohol phosphatidyltransferase family protein, at 3,597,548 to 3,598,180
_RS16340
LRK53_RS16345: LRK53_RS16345 - hypothetical protein, at 3,598,200 to 3,598,487
_RS16345
Position (kb)
3596
3597
3598 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.127 kb on + strand, within LRK53_RS16330 at 3596.128 kb on - strand, within LRK53_RS16330 at 3596.128 kb on - strand, within LRK53_RS16330 at 3596.325 kb on + strand at 3596.325 kb on + strand at 3596.325 kb on + strand at 3596.326 kb on - strand at 3596.486 kb on + strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3597.029 kb on + strand, within LRK53_RS16335 at 3597.063 kb on + strand, within LRK53_RS16335 at 3597.518 kb on - strand at 3597.802 kb on + strand, within LRK53_RS16340 at 3597.802 kb on + strand, within LRK53_RS16340 at 3597.803 kb on - strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.822 kb on - strand, within LRK53_RS16340 at 3597.822 kb on - strand, within LRK53_RS16340 at 3597.875 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.937 kb on + strand, within LRK53_RS16340 at 3597.938 kb on - strand, within LRK53_RS16340 at 3597.938 kb on - strand, within LRK53_RS16340 at 3598.093 kb on + strand, within LRK53_RS16340 at 3598.119 kb on - strand at 3598.192 kb on + strand at 3598.192 kb on + strand at 3598.192 kb on + strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.201 kb on + strand at 3598.333 kb on - strand, within LRK53_RS16345 at 3598.333 kb on - strand, within LRK53_RS16345 at 3598.333 kb on - strand, within LRK53_RS16345
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 3,595,594 + +1.4 3,595,594 + -0.6 3,595,594 + +0.1 3,595,594 + +0.1 3,595,594 + -1.4 3,595,594 + +0.4 3,595,594 + +0.9 3,595,594 + +1.4 3,595,594 + -0.2 3,595,594 + -0.9 3,595,594 + -0.2 3,595,594 + -1.0 3,595,594 + -0.1 3,595,594 + -0.4 3,595,594 + +2.4 3,595,594 + -0.5 3,595,594 + -1.2 3,595,594 + +0.4 3,595,595 - -1.0 3,595,595 - -0.2 3,595,595 - +0.2 3,595,595 - -0.8 3,595,595 - -0.2 3,595,595 - -0.7 3,595,595 - -0.2 3,595,595 - +1.4 3,595,595 - -1.0 3,595,595 - +2.4 3,595,595 - -0.8 3,595,595 - -0.5 3,595,595 - -1.1 3,595,595 - -0.0 3,595,595 - -0.8 3,595,595 - -1.5 3,596,070 + LRK53_RS16330 0.50 +0.4 3,596,070 + LRK53_RS16330 0.50 -1.6 3,596,070 + LRK53_RS16330 0.50 -0.6 3,596,070 + LRK53_RS16330 0.50 -1.0 3,596,070 + LRK53_RS16330 0.50 -2.6 3,596,070 + LRK53_RS16330 0.50 +0.4 3,596,070 + LRK53_RS16330 0.50 -0.2 3,596,070 + LRK53_RS16330 0.50 -0.6 3,596,070 + LRK53_RS16330 0.50 -0.6 3,596,070 + LRK53_RS16330 0.50 -0.4 3,596,070 + LRK53_RS16330 0.50 -0.2 3,596,070 + LRK53_RS16330 0.50 +1.4 3,596,071 - LRK53_RS16330 0.50 -0.6 3,596,071 - LRK53_RS16330 0.50 -1.4 3,596,071 - LRK53_RS16330 0.50 -2.9 3,596,071 - LRK53_RS16330 0.50 -3.9 3,596,071 - LRK53_RS16330 0.50 -3.3 3,596,071 - LRK53_RS16330 0.50 -0.6 3,596,071 - LRK53_RS16330 0.50 -1.5 3,596,071 - LRK53_RS16330 0.50 +0.4 3,596,071 - LRK53_RS16330 0.50 -1.6 3,596,071 - LRK53_RS16330 0.50 -2.7 3,596,071 - LRK53_RS16330 0.50 -1.0 3,596,071 - LRK53_RS16330 0.50 +0.4 3,596,071 - LRK53_RS16330 0.50 -1.6 3,596,071 - LRK53_RS16330 0.50 -0.1 3,596,071 - LRK53_RS16330 0.50 -1.5 3,596,071 - LRK53_RS16330 0.50 -0.7 3,596,071 - LRK53_RS16330 0.50 -0.2 3,596,127 + LRK53_RS16330 0.59 +1.4 3,596,128 - LRK53_RS16330 0.59 -1.8 3,596,128 - LRK53_RS16330 0.59 -3.2 3,596,325 + -0.6 3,596,325 + -1.0 3,596,325 + -0.6 3,596,326 - -1.6 3,596,486 + -1.2 3,596,487 - -0.6 3,596,487 - -2.4 3,596,487 - -1.0 3,596,487 - -3.5 3,596,487 - +1.4 3,596,487 - -1.2 3,596,487 - -1.2 3,596,487 - -1.1 3,596,687 + LRK53_RS16335 0.26 +0.0 3,596,687 + LRK53_RS16335 0.26 -2.3 3,596,687 + LRK53_RS16335 0.26 -0.2 3,596,687 + LRK53_RS16335 0.26 +0.2 3,596,687 + LRK53_RS16335 0.26 -1.4 3,596,688 - LRK53_RS16335 0.26 -2.0 3,596,688 - LRK53_RS16335 0.26 -1.2 3,596,688 - LRK53_RS16335 0.26 -2.4 3,596,688 - LRK53_RS16335 0.26 -0.2 3,596,688 - LRK53_RS16335 0.26 -1.2 3,596,688 - LRK53_RS16335 0.26 -2.5 3,596,688 - LRK53_RS16335 0.26 -3.2 3,596,826 + LRK53_RS16335 0.39 -0.2 3,596,826 + LRK53_RS16335 0.39 -0.0 3,596,826 + LRK53_RS16335 0.39 -0.2 3,596,826 + LRK53_RS16335 0.39 -1.6 3,596,827 - LRK53_RS16335 0.39 -2.2 3,596,827 - LRK53_RS16335 0.39 +0.4 3,596,827 - LRK53_RS16335 0.39 -1.2 3,596,827 - LRK53_RS16335 0.39 -1.0 3,596,827 - LRK53_RS16335 0.39 +1.4 3,596,827 - LRK53_RS16335 0.39 -0.2 3,597,029 + LRK53_RS16335 0.58 -0.6 3,597,063 + LRK53_RS16335 0.61 -1.4 3,597,518 - -1.4 3,597,802 + LRK53_RS16340 0.40 -0.6 3,597,802 + LRK53_RS16340 0.40 -1.2 3,597,803 - LRK53_RS16340 0.40 -0.2 3,597,821 + LRK53_RS16340 0.43 +1.4 3,597,821 + LRK53_RS16340 0.43 -1.2 3,597,821 + LRK53_RS16340 0.43 +0.2 3,597,821 + LRK53_RS16340 0.43 +0.1 3,597,821 + LRK53_RS16340 0.43 +0.4 3,597,822 - LRK53_RS16340 0.43 -0.9 3,597,822 - LRK53_RS16340 0.43 -0.9 3,597,875 + LRK53_RS16340 0.52 -0.2 3,597,935 + LRK53_RS16340 0.61 -0.6 3,597,935 + LRK53_RS16340 0.61 -1.4 3,597,935 + LRK53_RS16340 0.61 -1.6 3,597,935 + LRK53_RS16340 0.61 -0.2 3,597,935 + LRK53_RS16340 0.61 -0.9 3,597,935 + LRK53_RS16340 0.61 -1.4 3,597,935 + LRK53_RS16340 0.61 +0.2 3,597,935 + LRK53_RS16340 0.61 -0.2 3,597,935 + LRK53_RS16340 0.61 -0.2 3,597,935 + LRK53_RS16340 0.61 -0.8 3,597,936 - LRK53_RS16340 0.61 -0.6 3,597,936 - LRK53_RS16340 0.61 -0.6 3,597,936 - LRK53_RS16340 0.61 +0.4 3,597,936 - LRK53_RS16340 0.61 -1.2 3,597,936 - LRK53_RS16340 0.61 +0.4 3,597,936 - LRK53_RS16340 0.61 +2.0 3,597,936 - LRK53_RS16340 0.61 +1.4 3,597,936 - LRK53_RS16340 0.61 +1.4 3,597,936 - LRK53_RS16340 0.61 -1.4 3,597,936 - LRK53_RS16340 0.61 -0.6 3,597,936 - LRK53_RS16340 0.61 -0.2 3,597,936 - LRK53_RS16340 0.61 +0.5 3,597,936 - LRK53_RS16340 0.61 -0.6 3,597,936 - LRK53_RS16340 0.61 -1.7 3,597,937 + LRK53_RS16340 0.61 -0.6 3,597,938 - LRK53_RS16340 0.62 -0.4 3,597,938 - LRK53_RS16340 0.62 -1.3 3,598,093 + LRK53_RS16340 0.86 -1.2 3,598,119 - +1.4 3,598,192 + -0.6 3,598,192 + +0.8 3,598,192 + +0.8 3,598,193 - -0.2 3,598,193 - -0.9 3,598,193 - -1.1 3,598,193 - +1.4 3,598,193 - -3.1 3,598,201 + +0.1 3,598,333 - LRK53_RS16345 0.46 +0.7 3,598,333 - LRK53_RS16345 0.46 -1.2 3,598,333 - LRK53_RS16345 0.46 -0.6
Or see this region's nucleotide sequence