Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS12600 and LRK53_RS12605 are separated by 97 nucleotides LRK53_RS12605 and LRK53_RS12610 overlap by 29 nucleotides LRK53_RS12610 and LRK53_RS12615 are separated by 103 nucleotides
LRK53_RS12600: LRK53_RS12600 - lipoprotein-releasing ABC transporter permease subunit, at 2,791,228 to 2,792,469
_RS12600
LRK53_RS12605: LRK53_RS12605 - hypothetical protein, at 2,792,567 to 2,793,004
_RS12605
LRK53_RS12610: LRK53_RS12610 - succinate dehydrogenase assembly factor 2, at 2,792,976 to 2,793,224
_RS12610
LRK53_RS12615: LRK53_RS12615 - succinate dehydrogenase iron-sulfur subunit, at 2,793,328 to 2,794,110
_RS12615
Position (kb)
2792
2793
2794 Strain fitness (log2 ratio)
-1
0
1
2 at 2792.690 kb on - strand, within LRK53_RS12605 at 2792.718 kb on - strand, within LRK53_RS12605 at 2792.748 kb on - strand, within LRK53_RS12605 at 2792.748 kb on - strand, within LRK53_RS12605 at 2792.974 kb on - strand at 2793.242 kb on - strand at 2793.260 kb on + strand at 2793.260 kb on + strand at 2793.261 kb on - strand at 2793.261 kb on - strand at 2793.261 kb on - strand at 2793.261 kb on - strand at 2793.261 kb on - strand at 2793.261 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 2,792,690 - LRK53_RS12605 0.28 -1.6 2,792,718 - LRK53_RS12605 0.34 -1.2 2,792,748 - LRK53_RS12605 0.41 +1.1 2,792,748 - LRK53_RS12605 0.41 -1.5 2,792,974 - -1.7 2,793,242 - +0.1 2,793,260 + +0.3 2,793,260 + +2.1 2,793,261 - -1.0 2,793,261 - +0.7 2,793,261 - -0.7 2,793,261 - +1.1 2,793,261 - +1.1 2,793,261 - +0.1
Or see this region's nucleotide sequence