Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06235 and LRK53_RS06240 are separated by 48 nucleotides LRK53_RS06240 and LRK53_RS06245 are separated by 50 nucleotides LRK53_RS06245 and LRK53_RS06250 are separated by 163 nucleotides LRK53_RS06250 and LRK53_RS06255 are separated by 54 nucleotides
LRK53_RS06235: LRK53_RS06235 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 1,429,540 to 1,430,922
_RS06235
LRK53_RS06240: LRK53_RS06240 - tRNA-Gln, at 1,430,971 to 1,431,047
_RS06240
LRK53_RS06245: LRK53_RS06245 - ribose-phosphate diphosphokinase, at 1,431,098 to 1,432,039
_RS06245
LRK53_RS06250: LRK53_RS06250 - 50S ribosomal protein L25/general stress protein Ctc, at 1,432,203 to 1,432,874
_RS06250
LRK53_RS06255: LRK53_RS06255 - aminoacyl-tRNA hydrolase, at 1,432,929 to 1,433,513
_RS06255
Position (kb) 1431
1432
1433 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1430.957 kb on + strand at 1430.957 kb on + strand at 1430.957 kb on + strand at 1430.957 kb on + strand at 1430.957 kb on + strand at 1430.957 kb on + strand at 1430.957 kb on + strand at 1430.957 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1430.965 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand at 1431.073 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 1,430,957 + +2.1 1,430,957 + -0.3 1,430,957 + +0.2 1,430,957 + +0.8 1,430,957 + -1.1 1,430,957 + +1.1 1,430,957 + -1.3 1,430,957 + +0.1 1,430,965 + -0.7 1,430,965 + -1.0 1,430,965 + -0.2 1,430,965 + +1.1 1,430,965 + +0.1 1,430,965 + +0.3 1,430,965 + +1.1 1,430,965 + -1.3 1,430,965 + -1.4 1,430,965 + +1.1 1,430,965 + -2.5 1,430,965 + -0.3 1,430,965 + -0.4 1,430,965 + -0.7 1,430,965 + -1.0 1,430,965 + -0.3 1,430,965 + -1.1 1,430,965 + -0.9 1,430,965 + -0.5 1,430,965 + +1.3 1,430,965 + -0.6 1,430,965 + -0.9 1,430,965 + +1.1 1,430,965 + -0.7 1,430,965 + -1.5 1,430,965 + -0.2 1,430,965 + +1.1 1,430,965 + -1.5 1,430,965 + +0.1 1,430,965 + -1.4 1,430,965 + -3.2 1,430,965 + -0.8 1,430,965 + -1.8 1,430,965 + -0.9 1,430,965 + -0.1 1,430,965 + +2.1 1,430,965 + -1.6 1,430,965 + +0.9 1,430,965 + -0.2 1,430,965 + +0.5 1,430,965 + -0.8 1,430,965 + -0.8 1,430,965 + -1.2 1,430,965 + -0.1 1,430,965 + -0.3 1,430,965 + -0.5 1,430,965 + -0.9 1,430,965 + -1.1 1,430,965 + +0.3 1,430,965 + -0.5 1,430,965 + +0.5 1,430,965 + -1.8 1,430,965 + -0.5 1,430,965 + -0.7 1,430,965 + +1.1 1,430,965 + -0.3 1,430,965 + +0.1 1,430,965 + -0.3 1,430,965 + -0.5 1,430,965 + +0.1 1,431,073 + -1.3 1,431,073 + -1.3 1,431,073 + -2.6 1,431,073 + -0.5 1,431,073 + -0.9 1,431,073 + +2.1 1,431,073 + +0.1 1,431,073 + -1.9 1,431,073 + -3.0 1,431,073 + +0.1 1,431,073 + -3.7 1,431,073 + +0.1
Or see this region's nucleotide sequence