Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04265 and LRK53_RS04270 are separated by 127 nucleotides LRK53_RS04270 and LRK53_RS04275 overlap by 4 nucleotides LRK53_RS04275 and LRK53_RS04280 are separated by 133 nucleotides
LRK53_RS04265: LRK53_RS04265 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 993,623 to 994,519
_RS04265
LRK53_RS04270: LRK53_RS04270 - c-type cytochrome, at 994,647 to 994,955
_RS04270
LRK53_RS04275: LRK53_RS04275 - co-chaperone DjlA, at 994,952 to 995,758
_RS04275
LRK53_RS04280: LRK53_RS04280 - HAD hydrolase-like protein, at 995,892 to 996,530
_RS04280
Position (kb) 994
995 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 993.666 kb on + strand at 993.666 kb on + strand at 993.667 kb on - strand at 993.772 kb on - strand, within LRK53_RS04265 at 993.772 kb on - strand, within LRK53_RS04265 at 993.772 kb on - strand, within LRK53_RS04265 at 993.787 kb on + strand, within LRK53_RS04265 at 993.788 kb on - strand, within LRK53_RS04265 at 993.798 kb on + strand, within LRK53_RS04265 at 993.855 kb on + strand, within LRK53_RS04265 at 993.855 kb on + strand, within LRK53_RS04265 at 993.855 kb on + strand, within LRK53_RS04265 at 993.855 kb on + strand, within LRK53_RS04265 at 993.855 kb on + strand, within LRK53_RS04265 at 993.856 kb on - strand, within LRK53_RS04265 at 993.856 kb on - strand, within LRK53_RS04265 at 993.856 kb on - strand, within LRK53_RS04265 at 993.856 kb on - strand, within LRK53_RS04265 at 993.928 kb on - strand, within LRK53_RS04265 at 993.945 kb on + strand, within LRK53_RS04265 at 993.945 kb on + strand, within LRK53_RS04265 at 993.945 kb on + strand, within LRK53_RS04265 at 993.945 kb on + strand, within LRK53_RS04265 at 993.946 kb on - strand, within LRK53_RS04265 at 993.946 kb on - strand, within LRK53_RS04265 at 994.143 kb on + strand, within LRK53_RS04265 at 994.143 kb on + strand, within LRK53_RS04265 at 994.144 kb on - strand, within LRK53_RS04265 at 994.171 kb on - strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.236 kb on + strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.237 kb on - strand, within LRK53_RS04265 at 994.446 kb on + strand at 994.447 kb on - strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.448 kb on + strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.449 kb on - strand at 994.556 kb on + strand at 994.612 kb on + strand at 994.657 kb on + strand at 994.657 kb on + strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.658 kb on - strand at 994.812 kb on + strand, within LRK53_RS04270 at 994.812 kb on + strand, within LRK53_RS04270 at 994.812 kb on + strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.813 kb on - strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.923 kb on + strand, within LRK53_RS04270 at 994.924 kb on - strand, within LRK53_RS04270 at 994.924 kb on - strand, within LRK53_RS04270 at 994.924 kb on - strand, within LRK53_RS04270 at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.958 kb on + strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.959 kb on - strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.964 kb on + strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 994.965 kb on - strand at 995.061 kb on - strand, within LRK53_RS04275 at 995.425 kb on + strand, within LRK53_RS04275 at 995.468 kb on + strand, within LRK53_RS04275 at 995.510 kb on + strand, within LRK53_RS04275 at 995.510 kb on + strand, within LRK53_RS04275 at 995.511 kb on - strand, within LRK53_RS04275 at 995.529 kb on - strand, within LRK53_RS04275 at 995.567 kb on + strand, within LRK53_RS04275 at 995.568 kb on - strand, within LRK53_RS04275 at 995.723 kb on + strand at 995.723 kb on + strand at 995.724 kb on - strand at 995.757 kb on - strand at 995.757 kb on - strand at 995.757 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 993,666 + -0.5 993,666 + +0.1 993,667 - -0.9 993,772 - LRK53_RS04265 0.17 -1.9 993,772 - LRK53_RS04265 0.17 -1.7 993,772 - LRK53_RS04265 0.17 +0.1 993,787 + LRK53_RS04265 0.18 -1.2 993,788 - LRK53_RS04265 0.18 -0.5 993,798 + LRK53_RS04265 0.20 -0.9 993,855 + LRK53_RS04265 0.26 -1.2 993,855 + LRK53_RS04265 0.26 -0.9 993,855 + LRK53_RS04265 0.26 -3.1 993,855 + LRK53_RS04265 0.26 -0.5 993,855 + LRK53_RS04265 0.26 -0.5 993,856 - LRK53_RS04265 0.26 -2.4 993,856 - LRK53_RS04265 0.26 -2.4 993,856 - LRK53_RS04265 0.26 -1.7 993,856 - LRK53_RS04265 0.26 -1.7 993,928 - LRK53_RS04265 0.34 -2.4 993,945 + LRK53_RS04265 0.36 -1.2 993,945 + LRK53_RS04265 0.36 +0.1 993,945 + LRK53_RS04265 0.36 -3.1 993,945 + LRK53_RS04265 0.36 -2.2 993,946 - LRK53_RS04265 0.36 -2.4 993,946 - LRK53_RS04265 0.36 -1.7 994,143 + LRK53_RS04265 0.58 -0.5 994,143 + LRK53_RS04265 0.58 +0.1 994,144 - LRK53_RS04265 0.58 -1.2 994,171 - LRK53_RS04265 0.61 -2.6 994,236 + LRK53_RS04265 0.68 -2.2 994,236 + LRK53_RS04265 0.68 -1.7 994,236 + LRK53_RS04265 0.68 -0.9 994,236 + LRK53_RS04265 0.68 -1.5 994,236 + LRK53_RS04265 0.68 -0.5 994,236 + LRK53_RS04265 0.68 -1.8 994,236 + LRK53_RS04265 0.68 +0.1 994,236 + LRK53_RS04265 0.68 -1.5 994,237 - LRK53_RS04265 0.68 -1.2 994,237 - LRK53_RS04265 0.68 -0.9 994,237 - LRK53_RS04265 0.68 -1.2 994,237 - LRK53_RS04265 0.68 -1.5 994,237 - LRK53_RS04265 0.68 +0.1 994,237 - LRK53_RS04265 0.68 -3.2 994,446 + -0.9 994,447 - -2.1 994,448 + -2.6 994,448 + -1.7 994,448 + -2.1 994,448 + -1.7 994,448 + -1.2 994,448 + -1.2 994,448 + -0.9 994,448 + -1.5 994,449 - -1.7 994,449 - -0.5 994,449 - -0.5 994,449 - -2.2 994,449 - -0.9 994,449 - -1.7 994,449 - -3.2 994,449 - -1.5 994,449 - -4.0 994,449 - -0.9 994,449 - -0.5 994,556 + -0.7 994,612 + +0.1 994,657 + +2.1 994,657 + +0.7 994,658 - -0.5 994,658 - -1.7 994,658 - -0.5 994,658 - -0.5 994,658 - -2.1 994,812 + LRK53_RS04270 0.53 -1.7 994,812 + LRK53_RS04270 0.53 -2.2 994,812 + LRK53_RS04270 0.53 -1.7 994,813 - LRK53_RS04270 0.54 -2.2 994,813 - LRK53_RS04270 0.54 +0.1 994,813 - LRK53_RS04270 0.54 -0.9 994,813 - LRK53_RS04270 0.54 +1.1 994,813 - LRK53_RS04270 0.54 -0.5 994,923 + LRK53_RS04270 0.89 +0.1 994,923 + LRK53_RS04270 0.89 -1.9 994,923 + LRK53_RS04270 0.89 -0.9 994,923 + LRK53_RS04270 0.89 -2.9 994,924 - LRK53_RS04270 0.90 -0.9 994,924 - LRK53_RS04270 0.90 -1.5 994,924 - LRK53_RS04270 0.90 -0.2 994,958 + +0.4 994,958 + -0.8 994,958 + +0.1 994,958 + +0.1 994,958 + -0.2 994,958 + -1.3 994,958 + -0.9 994,958 + +0.1 994,958 + -0.2 994,958 + -0.9 994,958 + -0.9 994,958 + -1.9 994,958 + +2.4 994,958 + -1.8 994,959 - +0.5 994,959 - -0.4 994,959 - +0.1 994,959 - -1.7 994,959 - -1.2 994,959 - +1.1 994,959 - -0.6 994,959 - -1.7 994,959 - -0.9 994,959 - -0.7 994,959 - -1.5 994,959 - -0.6 994,959 - +0.1 994,964 + -0.5 994,964 + -0.9 994,964 + -0.9 994,964 + -0.9 994,964 + -0.5 994,964 + -0.6 994,964 + -0.5 994,964 + -0.4 994,964 + +0.5 994,964 + +1.7 994,964 + +1.1 994,964 + +0.5 994,964 + -0.9 994,964 + -0.9 994,964 + -0.9 994,964 + -2.3 994,964 + -0.5 994,964 + -0.5 994,965 - -1.7 994,965 - -2.1 994,965 - -1.3 994,965 - -0.6 994,965 - -2.5 994,965 - +1.1 994,965 - -0.8 994,965 - -1.4 994,965 - -0.3 994,965 - +0.4 994,965 - -1.4 994,965 - -0.9 994,965 - -0.5 994,965 - +0.1 994,965 - -1.7 994,965 - -1.5 995,061 - LRK53_RS04275 0.14 -0.4 995,425 + LRK53_RS04275 0.59 -0.5 995,468 + LRK53_RS04275 0.64 +0.3 995,510 + LRK53_RS04275 0.69 -0.5 995,510 + LRK53_RS04275 0.69 -1.5 995,511 - LRK53_RS04275 0.69 +1.1 995,529 - LRK53_RS04275 0.71 -1.7 995,567 + LRK53_RS04275 0.76 -1.0 995,568 - LRK53_RS04275 0.76 -1.7 995,723 + -1.5 995,723 + +0.1 995,724 - +0.1 995,757 - -0.5 995,757 - -0.2 995,757 - -1.1
Or see this region's nucleotide sequence