Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04045 and LRK53_RS04050 are separated by 87 nucleotides LRK53_RS04050 and LRK53_RS04055 are separated by 4 nucleotides LRK53_RS04055 and LRK53_RS04060 are separated by 157 nucleotides
LRK53_RS04045: LRK53_RS04045 - UDP-2,3-diacylglucosamine diphosphatase, at 947,882 to 948,754
_RS04045
LRK53_RS04050: LRK53_RS04050 - SGNH/GDSL hydrolase family protein, at 948,842 to 949,441
_RS04050
LRK53_RS04055: LRK53_RS04055 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 949,446 to 950,693
_RS04055
LRK53_RS04060: LRK53_RS04060 - ribosome small subunit-dependent GTPase A, at 950,851 to 951,906
_RS04060
Position (kb) 949
950
951 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.519 kb on + strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.520 kb on - strand, within LRK53_RS04045 at 948.639 kb on + strand, within LRK53_RS04045 at 948.640 kb on - strand, within LRK53_RS04045 at 948.640 kb on - strand, within LRK53_RS04045 at 948.640 kb on - strand, within LRK53_RS04045 at 948.640 kb on - strand, within LRK53_RS04045 at 948.681 kb on + strand at 948.681 kb on + strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.682 kb on - strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.897 kb on + strand at 948.898 kb on - strand at 948.898 kb on - strand at 948.898 kb on - strand at 948.898 kb on - strand at 948.898 kb on - strand at 948.898 kb on - strand at 949.020 kb on + strand, within LRK53_RS04050 at 949.020 kb on + strand, within LRK53_RS04050 at 949.020 kb on + strand, within LRK53_RS04050 at 949.020 kb on + strand, within LRK53_RS04050 at 949.020 kb on + strand, within LRK53_RS04050 at 949.020 kb on + strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.021 kb on - strand, within LRK53_RS04050 at 949.179 kb on + strand, within LRK53_RS04050 at 949.179 kb on + strand, within LRK53_RS04050 at 949.180 kb on - strand, within LRK53_RS04050 at 949.203 kb on + strand, within LRK53_RS04050 at 949.410 kb on + strand at 949.410 kb on + strand at 949.410 kb on + strand at 949.410 kb on + strand at 949.410 kb on + strand at 949.410 kb on + strand at 949.411 kb on - strand at 949.411 kb on - strand at 949.411 kb on - strand at 949.411 kb on - strand at 949.411 kb on - strand at 949.411 kb on - strand at 949.411 kb on - strand at 949.411 kb on - strand at 949.521 kb on + strand at 949.521 kb on + strand at 949.521 kb on + strand at 949.521 kb on + strand at 949.521 kb on + strand at 949.522 kb on - strand at 949.522 kb on - strand at 949.522 kb on - strand at 949.522 kb on - strand at 949.522 kb on - strand at 949.522 kb on - strand at 949.522 kb on - strand at 949.680 kb on + strand, within LRK53_RS04055 at 949.680 kb on + strand, within LRK53_RS04055 at 949.681 kb on - strand, within LRK53_RS04055 at 949.696 kb on + strand, within LRK53_RS04055 at 949.697 kb on - strand, within LRK53_RS04055 at 949.697 kb on - strand, within LRK53_RS04055 at 949.697 kb on - strand, within LRK53_RS04055 at 950.050 kb on + strand, within LRK53_RS04055 at 950.051 kb on - strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.065 kb on + strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.066 kb on - strand, within LRK53_RS04055 at 950.140 kb on + strand, within LRK53_RS04055 at 950.141 kb on - strand, within LRK53_RS04055 at 950.258 kb on - strand, within LRK53_RS04055 at 950.260 kb on + strand, within LRK53_RS04055 at 950.260 kb on + strand, within LRK53_RS04055 at 950.261 kb on - strand, within LRK53_RS04055 at 950.261 kb on - strand, within LRK53_RS04055 at 950.309 kb on - strand, within LRK53_RS04055 at 950.491 kb on + strand, within LRK53_RS04055 at 950.492 kb on - strand, within LRK53_RS04055 at 950.492 kb on - strand, within LRK53_RS04055 at 950.492 kb on - strand, within LRK53_RS04055 at 950.558 kb on - strand, within LRK53_RS04055 at 950.740 kb on + strand at 950.740 kb on + strand at 950.741 kb on - strand at 950.741 kb on - strand at 950.741 kb on - strand at 950.741 kb on - strand at 950.741 kb on - strand at 950.741 kb on - strand at 950.741 kb on - strand at 950.819 kb on + strand at 950.819 kb on + strand at 950.819 kb on + strand at 950.820 kb on - strand at 950.820 kb on - strand at 950.820 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 948,519 + LRK53_RS04045 0.73 -0.0 948,519 + LRK53_RS04045 0.73 +1.1 948,519 + LRK53_RS04045 0.73 +1.1 948,519 + LRK53_RS04045 0.73 -0.5 948,519 + LRK53_RS04045 0.73 -0.2 948,519 + LRK53_RS04045 0.73 +0.5 948,519 + LRK53_RS04045 0.73 -0.3 948,519 + LRK53_RS04045 0.73 -2.8 948,519 + LRK53_RS04045 0.73 -1.3 948,519 + LRK53_RS04045 0.73 -1.5 948,519 + LRK53_RS04045 0.73 -1.2 948,519 + LRK53_RS04045 0.73 -0.5 948,519 + LRK53_RS04045 0.73 +0.1 948,519 + LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 -0.5 948,520 - LRK53_RS04045 0.73 +0.7 948,520 - LRK53_RS04045 0.73 -0.5 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 -0.2 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 -0.5 948,520 - LRK53_RS04045 0.73 -0.9 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 -0.6 948,520 - LRK53_RS04045 0.73 +0.3 948,520 - LRK53_RS04045 0.73 -0.9 948,520 - LRK53_RS04045 0.73 +0.7 948,520 - LRK53_RS04045 0.73 -2.0 948,520 - LRK53_RS04045 0.73 -1.5 948,520 - LRK53_RS04045 0.73 +0.5 948,520 - LRK53_RS04045 0.73 +1.1 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 -0.2 948,520 - LRK53_RS04045 0.73 +1.1 948,520 - LRK53_RS04045 0.73 -0.6 948,520 - LRK53_RS04045 0.73 -1.7 948,520 - LRK53_RS04045 0.73 +1.1 948,520 - LRK53_RS04045 0.73 -0.5 948,520 - LRK53_RS04045 0.73 -1.7 948,520 - LRK53_RS04045 0.73 +1.5 948,520 - LRK53_RS04045 0.73 -0.9 948,520 - LRK53_RS04045 0.73 -0.5 948,520 - LRK53_RS04045 0.73 -2.1 948,520 - LRK53_RS04045 0.73 -1.2 948,520 - LRK53_RS04045 0.73 +1.1 948,520 - LRK53_RS04045 0.73 +0.1 948,520 - LRK53_RS04045 0.73 -1.4 948,520 - LRK53_RS04045 0.73 +0.1 948,639 + LRK53_RS04045 0.87 -1.3 948,640 - LRK53_RS04045 0.87 -0.5 948,640 - LRK53_RS04045 0.87 -0.9 948,640 - LRK53_RS04045 0.87 -1.0 948,640 - LRK53_RS04045 0.87 -0.3 948,681 + -1.5 948,681 + -2.1 948,682 - -1.7 948,682 - -0.7 948,682 - -0.2 948,682 - -1.5 948,682 - +0.1 948,682 - -0.6 948,897 + -0.9 948,897 + -1.6 948,897 + -0.7 948,897 + -1.9 948,897 + -1.0 948,897 + -0.5 948,897 + +1.1 948,897 + +0.1 948,897 + -0.6 948,897 + +0.9 948,897 + -0.0 948,898 - +0.5 948,898 - -0.5 948,898 - +1.1 948,898 - -1.5 948,898 - -0.5 948,898 - +1.1 949,020 + LRK53_RS04050 0.30 -1.2 949,020 + LRK53_RS04050 0.30 -0.5 949,020 + LRK53_RS04050 0.30 -1.3 949,020 + LRK53_RS04050 0.30 +0.1 949,020 + LRK53_RS04050 0.30 -1.1 949,020 + LRK53_RS04050 0.30 -0.8 949,021 - LRK53_RS04050 0.30 +0.1 949,021 - LRK53_RS04050 0.30 -0.5 949,021 - LRK53_RS04050 0.30 +0.4 949,021 - LRK53_RS04050 0.30 +0.4 949,021 - LRK53_RS04050 0.30 -1.7 949,021 - LRK53_RS04050 0.30 -1.7 949,021 - LRK53_RS04050 0.30 +1.1 949,021 - LRK53_RS04050 0.30 +0.1 949,021 - LRK53_RS04050 0.30 +0.1 949,021 - LRK53_RS04050 0.30 -0.7 949,021 - LRK53_RS04050 0.30 -2.7 949,021 - LRK53_RS04050 0.30 -0.7 949,179 + LRK53_RS04050 0.56 +0.5 949,179 + LRK53_RS04050 0.56 -0.9 949,180 - LRK53_RS04050 0.56 -1.9 949,203 + LRK53_RS04050 0.60 +0.1 949,410 + -0.5 949,410 + -0.9 949,410 + -1.0 949,410 + +0.2 949,410 + -0.5 949,410 + +1.1 949,411 - -0.9 949,411 - -1.2 949,411 - +1.1 949,411 - -0.5 949,411 - -1.9 949,411 - -0.9 949,411 - +1.1 949,411 - -1.1 949,521 + +0.1 949,521 + -1.1 949,521 + -1.4 949,521 + +2.1 949,521 + -1.3 949,522 - +0.1 949,522 - +0.1 949,522 - -1.4 949,522 - -2.0 949,522 - -2.7 949,522 - -1.2 949,522 - -1.2 949,680 + LRK53_RS04055 0.19 +0.1 949,680 + LRK53_RS04055 0.19 -0.5 949,681 - LRK53_RS04055 0.19 +0.1 949,696 + LRK53_RS04055 0.20 -1.5 949,697 - LRK53_RS04055 0.20 -0.2 949,697 - LRK53_RS04055 0.20 -1.9 949,697 - LRK53_RS04055 0.20 -1.5 950,050 + LRK53_RS04055 0.48 -1.9 950,051 - LRK53_RS04055 0.48 +0.1 950,065 + LRK53_RS04055 0.50 -1.2 950,065 + LRK53_RS04055 0.50 -1.7 950,065 + LRK53_RS04055 0.50 -1.2 950,065 + LRK53_RS04055 0.50 -1.4 950,065 + LRK53_RS04055 0.50 +0.4 950,065 + LRK53_RS04055 0.50 -2.2 950,065 + LRK53_RS04055 0.50 -0.5 950,065 + LRK53_RS04055 0.50 -0.5 950,065 + LRK53_RS04055 0.50 -1.7 950,066 - LRK53_RS04055 0.50 -2.6 950,066 - LRK53_RS04055 0.50 -0.5 950,066 - LRK53_RS04055 0.50 -0.5 950,066 - LRK53_RS04055 0.50 +0.1 950,066 - LRK53_RS04055 0.50 -0.5 950,066 - LRK53_RS04055 0.50 +0.1 950,066 - LRK53_RS04055 0.50 -0.5 950,066 - LRK53_RS04055 0.50 +1.1 950,140 + LRK53_RS04055 0.56 -2.0 950,141 - LRK53_RS04055 0.56 -1.0 950,258 - LRK53_RS04055 0.65 -0.5 950,260 + LRK53_RS04055 0.65 -0.9 950,260 + LRK53_RS04055 0.65 -1.3 950,261 - LRK53_RS04055 0.65 +1.1 950,261 - LRK53_RS04055 0.65 -1.9 950,309 - LRK53_RS04055 0.69 +0.1 950,491 + LRK53_RS04055 0.84 -3.4 950,492 - LRK53_RS04055 0.84 -0.5 950,492 - LRK53_RS04055 0.84 +1.1 950,492 - LRK53_RS04055 0.84 +0.1 950,558 - LRK53_RS04055 0.89 -0.9 950,740 + -0.5 950,740 + +1.1 950,741 - -0.2 950,741 - +0.1 950,741 - -0.5 950,741 - -1.2 950,741 - -0.3 950,741 - -0.9 950,741 - -0.5 950,819 + -0.2 950,819 + -0.5 950,819 + -2.2 950,820 - -0.5 950,820 - +0.5 950,820 - -0.5
Or see this region's nucleotide sequence