Experiment: defined media with 20AA_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS01755 and LRK53_RS01760 are separated by 181 nucleotides LRK53_RS01760 and LRK53_RS01765 are separated by 65 nucleotides LRK53_RS01765 and LRK53_RS01770 are separated by 95 nucleotides
LRK53_RS01755: LRK53_RS01755 - zinc-binding alcohol dehydrogenase family protein, at 410,991 to 412,007
_RS01755
LRK53_RS01760: LRK53_RS01760 - DUF2333 family protein, at 412,189 to 413,211
_RS01760
LRK53_RS01765: LRK53_RS01765 - inorganic diphosphatase, at 413,277 to 413,816
_RS01765
LRK53_RS01770: LRK53_RS01770 - 2OG-Fe(II) oxygenase, at 413,912 to 414,766
_RS01770
Position (kb) 412
413
414 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 411.232 kb on + strand, within LRK53_RS01755 at 411.232 kb on + strand, within LRK53_RS01755 at 411.232 kb on + strand, within LRK53_RS01755 at 411.232 kb on + strand, within LRK53_RS01755 at 411.232 kb on + strand, within LRK53_RS01755 at 411.232 kb on + strand, within LRK53_RS01755 at 411.233 kb on - strand, within LRK53_RS01755 at 411.233 kb on - strand, within LRK53_RS01755 at 411.233 kb on - strand, within LRK53_RS01755 at 411.233 kb on - strand, within LRK53_RS01755 at 411.233 kb on - strand, within LRK53_RS01755 at 411.322 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.603 kb on + strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.604 kb on - strand, within LRK53_RS01755 at 411.742 kb on + strand, within LRK53_RS01755 at 411.742 kb on + strand, within LRK53_RS01755 at 411.742 kb on + strand, within LRK53_RS01755 at 411.742 kb on + strand, within LRK53_RS01755 at 411.742 kb on + strand, within LRK53_RS01755 at 411.743 kb on - strand, within LRK53_RS01755 at 411.745 kb on + strand, within LRK53_RS01755 at 411.745 kb on + strand, within LRK53_RS01755 at 411.745 kb on + strand, within LRK53_RS01755 at 411.746 kb on - strand, within LRK53_RS01755 at 411.746 kb on - strand, within LRK53_RS01755 at 411.746 kb on - strand, within LRK53_RS01755 at 411.795 kb on + strand, within LRK53_RS01755 at 411.796 kb on - strand, within LRK53_RS01755 at 411.796 kb on - strand, within LRK53_RS01755 at 411.982 kb on + strand at 411.983 kb on - strand at 411.983 kb on - strand at 411.983 kb on - strand at 412.089 kb on + strand at 412.090 kb on - strand at 412.090 kb on - strand at 412.156 kb on + strand at 412.156 kb on + strand at 412.156 kb on + strand at 412.156 kb on + strand at 412.156 kb on + strand at 412.156 kb on + strand at 412.203 kb on + strand at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.413 kb on + strand, within LRK53_RS01760 at 412.414 kb on - strand, within LRK53_RS01760 at 412.414 kb on - strand, within LRK53_RS01760 at 412.414 kb on - strand, within LRK53_RS01760 at 412.414 kb on - strand, within LRK53_RS01760 at 412.414 kb on - strand, within LRK53_RS01760 at 412.687 kb on + strand, within LRK53_RS01760 at 412.687 kb on + strand, within LRK53_RS01760 at 412.687 kb on + strand, within LRK53_RS01760 at 412.688 kb on - strand, within LRK53_RS01760 at 412.688 kb on - strand, within LRK53_RS01760 at 412.688 kb on - strand, within LRK53_RS01760 at 412.688 kb on - strand, within LRK53_RS01760 at 412.688 kb on - strand, within LRK53_RS01760 at 412.732 kb on + strand, within LRK53_RS01760 at 412.732 kb on + strand, within LRK53_RS01760 at 412.733 kb on - strand, within LRK53_RS01760 at 412.733 kb on - strand, within LRK53_RS01760 at 412.733 kb on - strand, within LRK53_RS01760 at 412.733 kb on - strand, within LRK53_RS01760 at 412.733 kb on - strand, within LRK53_RS01760 at 412.733 kb on - strand, within LRK53_RS01760 at 412.759 kb on + strand, within LRK53_RS01760 at 412.759 kb on + strand, within LRK53_RS01760 at 412.759 kb on + strand, within LRK53_RS01760 at 412.759 kb on + strand, within LRK53_RS01760 at 412.759 kb on + strand, within LRK53_RS01760 at 412.759 kb on + strand, within LRK53_RS01760 at 412.760 kb on - strand, within LRK53_RS01760 at 412.760 kb on - strand, within LRK53_RS01760 at 412.760 kb on - strand, within LRK53_RS01760 at 412.760 kb on - strand, within LRK53_RS01760 at 412.760 kb on - strand, within LRK53_RS01760 at 412.806 kb on - strand, within LRK53_RS01760 at 412.939 kb on + strand, within LRK53_RS01760 at 412.939 kb on + strand, within LRK53_RS01760 at 412.939 kb on + strand, within LRK53_RS01760 at 412.939 kb on + strand, within LRK53_RS01760 at 412.940 kb on - strand, within LRK53_RS01760 at 412.954 kb on + strand, within LRK53_RS01760 at 412.955 kb on - strand, within LRK53_RS01760 at 412.955 kb on - strand, within LRK53_RS01760 at 412.955 kb on - strand, within LRK53_RS01760 at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.152 kb on + strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.153 kb on - strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.256 kb on + strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.257 kb on - strand at 413.860 kb on + strand at 413.860 kb on + strand at 413.860 kb on + strand at 413.860 kb on + strand at 413.860 kb on + strand at 413.860 kb on + strand at 413.861 kb on - strand at 413.861 kb on - strand at 414.036 kb on + strand, within LRK53_RS01770 at 414.036 kb on + strand, within LRK53_RS01770 at 414.036 kb on + strand, within LRK53_RS01770 at 414.036 kb on + strand, within LRK53_RS01770 at 414.037 kb on - strand, within LRK53_RS01770 at 414.037 kb on - strand, within LRK53_RS01770 at 414.037 kb on - strand, within LRK53_RS01770 at 414.037 kb on - strand, within LRK53_RS01770 at 414.037 kb on - strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.120 kb on + strand, within LRK53_RS01770 at 414.121 kb on - strand, within LRK53_RS01770 at 414.121 kb on - strand, within LRK53_RS01770 at 414.121 kb on - strand, within LRK53_RS01770 at 414.121 kb on - strand, within LRK53_RS01770
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 20AA_mix remove 411,232 + LRK53_RS01755 0.24 +0.3 411,232 + LRK53_RS01755 0.24 -1.0 411,232 + LRK53_RS01755 0.24 +0.3 411,232 + LRK53_RS01755 0.24 -2.4 411,232 + LRK53_RS01755 0.24 +0.4 411,232 + LRK53_RS01755 0.24 +0.9 411,233 - LRK53_RS01755 0.24 -1.0 411,233 - LRK53_RS01755 0.24 +1.3 411,233 - LRK53_RS01755 0.24 +0.3 411,233 - LRK53_RS01755 0.24 -0.7 411,233 - LRK53_RS01755 0.24 -1.1 411,322 + LRK53_RS01755 0.33 -1.0 411,603 + LRK53_RS01755 0.60 -0.5 411,603 + LRK53_RS01755 0.60 +0.3 411,603 + LRK53_RS01755 0.60 -1.0 411,603 + LRK53_RS01755 0.60 -0.3 411,603 + LRK53_RS01755 0.60 -0.1 411,603 + LRK53_RS01755 0.60 -0.7 411,603 + LRK53_RS01755 0.60 -1.1 411,603 + LRK53_RS01755 0.60 -0.7 411,604 - LRK53_RS01755 0.60 +0.9 411,604 - LRK53_RS01755 0.60 -0.3 411,604 - LRK53_RS01755 0.60 -0.3 411,604 - LRK53_RS01755 0.60 -0.6 411,604 - LRK53_RS01755 0.60 -1.9 411,604 - LRK53_RS01755 0.60 +0.1 411,604 - LRK53_RS01755 0.60 -0.8 411,604 - LRK53_RS01755 0.60 +0.3 411,604 - LRK53_RS01755 0.60 -1.0 411,604 - LRK53_RS01755 0.60 -1.5 411,604 - LRK53_RS01755 0.60 -1.3 411,604 - LRK53_RS01755 0.60 -1.4 411,604 - LRK53_RS01755 0.60 -1.0 411,604 - LRK53_RS01755 0.60 -0.0 411,604 - LRK53_RS01755 0.60 -0.7 411,604 - LRK53_RS01755 0.60 -1.0 411,604 - LRK53_RS01755 0.60 -1.2 411,604 - LRK53_RS01755 0.60 +0.9 411,604 - LRK53_RS01755 0.60 +1.3 411,742 + LRK53_RS01755 0.74 -1.5 411,742 + LRK53_RS01755 0.74 -0.7 411,742 + LRK53_RS01755 0.74 -0.3 411,742 + LRK53_RS01755 0.74 +0.3 411,742 + LRK53_RS01755 0.74 +0.3 411,743 - LRK53_RS01755 0.74 +0.3 411,745 + LRK53_RS01755 0.74 +1.9 411,745 + LRK53_RS01755 0.74 -1.7 411,745 + LRK53_RS01755 0.74 -0.7 411,746 - LRK53_RS01755 0.74 -0.7 411,746 - LRK53_RS01755 0.74 -0.9 411,746 - LRK53_RS01755 0.74 -0.8 411,795 + LRK53_RS01755 0.79 -0.3 411,796 - LRK53_RS01755 0.79 -1.3 411,796 - LRK53_RS01755 0.79 -1.9 411,982 + +1.3 411,983 - +0.0 411,983 - -1.4 411,983 - +0.6 412,089 + -1.0 412,090 - -1.3 412,090 - -2.7 412,156 + -0.7 412,156 + -0.3 412,156 + -0.3 412,156 + -0.3 412,156 + -1.0 412,156 + -1.7 412,203 + -1.0 412,413 + LRK53_RS01760 0.22 -1.5 412,413 + LRK53_RS01760 0.22 -0.7 412,413 + LRK53_RS01760 0.22 -1.7 412,413 + LRK53_RS01760 0.22 -0.6 412,413 + LRK53_RS01760 0.22 +0.6 412,413 + LRK53_RS01760 0.22 -0.6 412,413 + LRK53_RS01760 0.22 -0.0 412,413 + LRK53_RS01760 0.22 -0.7 412,413 + LRK53_RS01760 0.22 +0.6 412,414 - LRK53_RS01760 0.22 +0.3 412,414 - LRK53_RS01760 0.22 +0.6 412,414 - LRK53_RS01760 0.22 -1.0 412,414 - LRK53_RS01760 0.22 -0.3 412,414 - LRK53_RS01760 0.22 -0.7 412,687 + LRK53_RS01760 0.49 -0.9 412,687 + LRK53_RS01760 0.49 -1.1 412,687 + LRK53_RS01760 0.49 -1.0 412,688 - LRK53_RS01760 0.49 -0.0 412,688 - LRK53_RS01760 0.49 -0.8 412,688 - LRK53_RS01760 0.49 +0.3 412,688 - LRK53_RS01760 0.49 -1.7 412,688 - LRK53_RS01760 0.49 -1.3 412,732 + LRK53_RS01760 0.53 -0.9 412,732 + LRK53_RS01760 0.53 +0.7 412,733 - LRK53_RS01760 0.53 -0.0 412,733 - LRK53_RS01760 0.53 -0.0 412,733 - LRK53_RS01760 0.53 -2.5 412,733 - LRK53_RS01760 0.53 -1.8 412,733 - LRK53_RS01760 0.53 -0.3 412,733 - LRK53_RS01760 0.53 -1.2 412,759 + LRK53_RS01760 0.56 -1.0 412,759 + LRK53_RS01760 0.56 +0.3 412,759 + LRK53_RS01760 0.56 -0.9 412,759 + LRK53_RS01760 0.56 -0.3 412,759 + LRK53_RS01760 0.56 -0.7 412,759 + LRK53_RS01760 0.56 +0.3 412,760 - LRK53_RS01760 0.56 -1.7 412,760 - LRK53_RS01760 0.56 -0.0 412,760 - LRK53_RS01760 0.56 -0.4 412,760 - LRK53_RS01760 0.56 -1.5 412,760 - LRK53_RS01760 0.56 +0.3 412,806 - LRK53_RS01760 0.60 +0.3 412,939 + LRK53_RS01760 0.73 +0.3 412,939 + LRK53_RS01760 0.73 -1.5 412,939 + LRK53_RS01760 0.73 -2.4 412,939 + LRK53_RS01760 0.73 -0.8 412,940 - LRK53_RS01760 0.73 -0.0 412,954 + LRK53_RS01760 0.75 -2.2 412,955 - LRK53_RS01760 0.75 +1.3 412,955 - LRK53_RS01760 0.75 -0.4 412,955 - LRK53_RS01760 0.75 -1.5 413,152 + -1.6 413,152 + +0.7 413,152 + -0.0 413,152 + -1.5 413,152 + +0.7 413,152 + -1.0 413,152 + +0.7 413,152 + +1.3 413,152 + -0.7 413,152 + -0.8 413,152 + -0.7 413,152 + +1.3 413,152 + -0.7 413,152 + +2.3 413,152 + -0.8 413,152 + -1.1 413,152 + +1.3 413,152 + +0.6 413,152 + +1.3 413,152 + +0.1 413,153 - -2.7 413,153 - -1.3 413,153 - +0.7 413,153 - +0.3 413,153 - -0.1 413,153 - -2.8 413,153 - +3.6 413,153 - +0.3 413,153 - +0.5 413,153 - -0.5 413,153 - -0.7 413,153 - -1.0 413,153 - -0.0 413,153 - +0.3 413,153 - -0.9 413,153 - +0.3 413,153 - -1.8 413,153 - +1.3 413,153 - -1.0 413,153 - -0.5 413,153 - -1.3 413,153 - -0.3 413,153 - -1.0 413,153 - +0.6 413,153 - +0.7 413,153 - -0.2 413,153 - -1.3 413,256 + +0.8 413,256 + -0.3 413,256 + -0.3 413,256 + -0.3 413,256 + +0.5 413,256 + -0.6 413,256 + +1.3 413,256 + -1.0 413,256 + +0.3 413,256 + -1.2 413,256 + -0.7 413,256 + -1.4 413,256 + -1.5 413,256 + -0.3 413,256 + -0.1 413,256 + -0.5 413,256 + -0.3 413,256 + -1.5 413,256 + +0.3 413,256 + -0.3 413,256 + +0.3 413,256 + -1.1 413,256 + +0.3 413,256 + -1.7 413,256 + +0.1 413,256 + -0.8 413,256 + -0.9 413,256 + -1.0 413,256 + +1.3 413,257 - +0.2 413,257 - -0.5 413,257 - -0.3 413,257 - -0.6 413,257 - -1.4 413,257 - +0.1 413,257 - -1.3 413,257 - +1.3 413,257 - -0.7 413,257 - -2.8 413,257 - -1.0 413,257 - -1.1 413,257 - -0.8 413,257 - +0.7 413,257 - +3.6 413,257 - +0.3 413,257 - -0.8 413,257 - -1.0 413,257 - +0.6 413,257 - -1.0 413,257 - +1.3 413,257 - -0.8 413,257 - -1.0 413,257 - -1.5 413,257 - -1.1 413,257 - -0.4 413,257 - -0.7 413,860 + -0.5 413,860 + -1.5 413,860 + +0.3 413,860 + -1.0 413,860 + -0.3 413,860 + +1.3 413,861 - -0.9 413,861 - +0.7 414,036 + LRK53_RS01770 0.15 -1.8 414,036 + LRK53_RS01770 0.15 +2.9 414,036 + LRK53_RS01770 0.15 -1.3 414,036 + LRK53_RS01770 0.15 -0.3 414,037 - LRK53_RS01770 0.15 -1.6 414,037 - LRK53_RS01770 0.15 +1.3 414,037 - LRK53_RS01770 0.15 -0.9 414,037 - LRK53_RS01770 0.15 +0.3 414,037 - LRK53_RS01770 0.15 -2.0 414,120 + LRK53_RS01770 0.24 -2.0 414,120 + LRK53_RS01770 0.24 -0.3 414,120 + LRK53_RS01770 0.24 -1.7 414,120 + LRK53_RS01770 0.24 -0.7 414,120 + LRK53_RS01770 0.24 +1.9 414,120 + LRK53_RS01770 0.24 +1.3 414,120 + LRK53_RS01770 0.24 -0.1 414,120 + LRK53_RS01770 0.24 +0.6 414,120 + LRK53_RS01770 0.24 -1.0 414,121 - LRK53_RS01770 0.24 -0.5 414,121 - LRK53_RS01770 0.24 -1.4 414,121 - LRK53_RS01770 0.24 -1.3 414,121 - LRK53_RS01770 0.24 -0.4
Or see this region's nucleotide sequence