Strain Fitness in Escherichia coli ECRC98 around JDDGAC_18945

Experiment: WM_K30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntetgA and grlR are separated by 195 nucleotidesgrlR and grlA are separated by 54 nucleotidesgrlA and cesD are separated by 383 nucleotidescesD and escC are separated by 13 nucleotides JDDGAC_18935: etgA - type III secretion system LEE muramidase EtgA, at 3,602,731 to 3,603,189 etgA JDDGAC_18940: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 3,603,385 to 3,603,756 grlR JDDGAC_18945: grlA - type III secretion system LEE transcriptional regulator GrlA, at 3,603,811 to 3,604,224 grlA JDDGAC_18950: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 3,604,608 to 3,605,063 cesD JDDGAC_18955: escC - type III secretion system LEE outer membrane ring protein EscC, at 3,605,077 to 3,606,615 escC Position (kb) 3603 3604 3605Strain fitness (log2 ratio) -2 -1 0 1 2at 3603.004 kb on - strand, within etgAat 3603.229 kb on + strandat 3603.407 kb on - strandat 3603.420 kb on + strandat 3603.537 kb on - strand, within grlRat 3603.553 kb on + strand, within grlRat 3603.556 kb on - strand, within grlRat 3603.906 kb on + strand, within grlAat 3604.602 kb on + strandat 3604.602 kb on + strandat 3604.623 kb on + strandat 3604.780 kb on - strand, within cesDat 3604.903 kb on - strand, within cesDat 3605.034 kb on - strandat 3605.164 kb on + strandat 3605.211 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_K30
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3,603,004 - etgA JDDGAC_18935 0.59 +0.7
3,603,229 + +2.0
3,603,407 - -0.7
3,603,420 + -0.4
3,603,537 - grlR JDDGAC_18940 0.41 +0.9
3,603,553 + grlR JDDGAC_18940 0.45 -1.6
3,603,556 - grlR JDDGAC_18940 0.46 -1.6
3,603,906 + grlA JDDGAC_18945 0.23 -1.5
3,604,602 + -1.9
3,604,602 + +0.5
3,604,623 + -0.0
3,604,780 - cesD JDDGAC_18950 0.38 -0.3
3,604,903 - cesD JDDGAC_18950 0.65 -0.2
3,605,034 - -0.9
3,605,164 + +1.6
3,605,211 + -1.4

Or see this region's nucleotide sequence