Strain Fitness in Escherichia coli ECRC98 around JDDGAC_06120

Experiment: WM_K30

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsirB1 and sirB2 are separated by 3 nucleotidessirB2 and prmC overlap by 4 nucleotidesprmC and prfA overlap by 1 nucleotides JDDGAC_06110: sirB1 - invasion regulator SirB1, at 1,063,537 to 1,064,346 sirB1 JDDGAC_06115: sirB2 - invasion regulator SirB2, at 1,064,350 to 1,064,742 sirB2 JDDGAC_06120: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 1,064,739 to 1,065,572 prmC JDDGAC_06125: prfA - peptide chain release factor 1, at 1,065,572 to 1,066,654 prfA Position (kb) 1064 1065 1066Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1063.880 kb on - strand, within sirB1at 1063.886 kb on - strand, within sirB1at 1063.886 kb on - strand, within sirB1at 1063.897 kb on - strand, within sirB1at 1063.897 kb on - strand, within sirB1at 1063.930 kb on - strand, within sirB1at 1064.033 kb on - strand, within sirB1at 1064.033 kb on - strand, within sirB1at 1064.045 kb on - strand, within sirB1at 1064.131 kb on - strand, within sirB1at 1064.386 kb on - strandat 1064.473 kb on - strand, within sirB2at 1064.473 kb on - strand, within sirB2at 1064.490 kb on - strand, within sirB2at 1064.490 kb on - strand, within sirB2at 1064.490 kb on - strand, within sirB2at 1064.581 kb on + strand, within sirB2at 1064.703 kb on + strandat 1064.704 kb on - strandat 1064.706 kb on + strandat 1065.015 kb on - strand, within prmCat 1065.400 kb on - strand, within prmCat 1065.553 kb on - strandat 1066.052 kb on - strand, within prfAat 1066.141 kb on - strand, within prfA

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_K30
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1,063,880 - sirB1 JDDGAC_06110 0.42 -1.8
1,063,886 - sirB1 JDDGAC_06110 0.43 -1.6
1,063,886 - sirB1 JDDGAC_06110 0.43 -1.1
1,063,897 - sirB1 JDDGAC_06110 0.44 +2.0
1,063,897 - sirB1 JDDGAC_06110 0.44 -0.5
1,063,930 - sirB1 JDDGAC_06110 0.49 +0.2
1,064,033 - sirB1 JDDGAC_06110 0.61 -0.5
1,064,033 - sirB1 JDDGAC_06110 0.61 +1.3
1,064,045 - sirB1 JDDGAC_06110 0.63 -1.7
1,064,131 - sirB1 JDDGAC_06110 0.73 -0.4
1,064,386 - -1.5
1,064,473 - sirB2 JDDGAC_06115 0.31 +0.7
1,064,473 - sirB2 JDDGAC_06115 0.31 +0.2
1,064,490 - sirB2 JDDGAC_06115 0.36 +0.7
1,064,490 - sirB2 JDDGAC_06115 0.36 +0.6
1,064,490 - sirB2 JDDGAC_06115 0.36 -3.7
1,064,581 + sirB2 JDDGAC_06115 0.59 +0.3
1,064,703 + +1.4
1,064,704 - -0.3
1,064,706 + +1.5
1,065,015 - prmC JDDGAC_06120 0.33 -1.0
1,065,400 - prmC JDDGAC_06120 0.79 -2.4
1,065,553 - -0.3
1,066,052 - prfA JDDGAC_06125 0.44 -3.8
1,066,141 - prfA JDDGAC_06125 0.53 -1.9

Or see this region's nucleotide sequence