Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trg and JDDGAC_04160 are separated by 340 nucleotides JDDGAC_04160 and yncP are separated by 17 nucleotides yncP and ydcA are separated by 25 nucleotides ydcA and cybB are separated by 244 nucleotides cybB and gap are separated by 188 nucleotides
JDDGAC_04150: trg - methyl-accepting chemotaxis protein Trg, at 727,611 to 729,251
trg
JDDGAC_04160: JDDGAC_04160 - Regulatory protein mokB, at 729,592 to 729,759
_04160
JDDGAC_04165: yncP - Protein YncP, at 729,777 to 729,845
yncP
JDDGAC_04170: ydcA - Uncharacterized protein YdcA, at 729,871 to 730,044
ydcA
JDDGAC_04180: cybB - cytochrome b561, at 730,289 to 730,819
cybB
JDDGAC_04185: gap - type I glyceraldehyde-3-phosphate dehydrogenase, at 731,008 to 732,009
gap
Position (kb)
729
730
731 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 728.942 kb on + strand, within trg at 728.943 kb on - strand, within trg at 728.966 kb on + strand, within trg at 729.023 kb on + strand, within trg at 729.024 kb on - strand, within trg at 729.160 kb on - strand at 729.171 kb on - strand at 729.177 kb on + strand at 729.285 kb on - strand at 729.312 kb on + strand at 729.313 kb on - strand at 729.492 kb on + strand at 729.492 kb on + strand at 729.492 kb on + strand at 729.493 kb on - strand at 729.493 kb on - strand at 729.685 kb on + strand, within JDDGAC_04160 at 729.797 kb on + strand, within yncP at 729.903 kb on + strand, within ydcA at 729.940 kb on - strand, within ydcA at 730.027 kb on - strand at 730.045 kb on - strand at 730.046 kb on + strand at 730.046 kb on + strand at 730.047 kb on - strand at 730.047 kb on - strand at 730.070 kb on + strand at 730.071 kb on - strand at 730.089 kb on - strand at 730.205 kb on - strand at 730.205 kb on - strand at 730.207 kb on - strand at 730.279 kb on + strand at 730.316 kb on + strand at 730.317 kb on - strand at 730.317 kb on - strand at 730.317 kb on - strand at 730.431 kb on - strand, within cybB at 730.503 kb on + strand, within cybB at 730.504 kb on - strand, within cybB at 730.504 kb on - strand, within cybB at 730.548 kb on + strand, within cybB at 730.605 kb on - strand, within cybB at 730.750 kb on - strand, within cybB at 730.835 kb on - strand at 730.881 kb on - strand at 730.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 728,942 + trg JDDGAC_04150 0.81 -3.0 728,943 - trg JDDGAC_04150 0.81 +0.8 728,966 + trg JDDGAC_04150 0.83 +0.5 729,023 + trg JDDGAC_04150 0.86 -0.4 729,024 - trg JDDGAC_04150 0.86 +2.4 729,160 - -0.2 729,171 - +0.5 729,177 + +2.8 729,285 - -1.3 729,312 + -0.7 729,313 - -0.3 729,492 + -1.2 729,492 + +0.4 729,492 + +1.0 729,493 - +0.7 729,493 - -2.6 729,685 + JDDGAC_04160 0.55 +0.9 729,797 + yncP JDDGAC_04165 0.29 +0.9 729,903 + ydcA JDDGAC_04170 0.18 -3.3 729,940 - ydcA JDDGAC_04170 0.40 +0.0 730,027 - -1.8 730,045 - +1.3 730,046 + -0.0 730,046 + -0.8 730,047 - +3.4 730,047 - -0.1 730,070 + +0.2 730,071 - +0.6 730,089 - +0.1 730,205 - +1.1 730,205 - -2.6 730,207 - -1.3 730,279 + +0.8 730,316 + +0.5 730,317 - +0.2 730,317 - +0.5 730,317 - -3.0 730,431 - cybB JDDGAC_04180 0.27 +0.1 730,503 + cybB JDDGAC_04180 0.40 +2.1 730,504 - cybB JDDGAC_04180 0.40 +1.3 730,504 - cybB JDDGAC_04180 0.40 +0.4 730,548 + cybB JDDGAC_04180 0.49 +0.8 730,605 - cybB JDDGAC_04180 0.60 +0.2 730,750 - cybB JDDGAC_04180 0.87 +1.2 730,835 - -0.6 730,881 - +0.6 730,885 - +0.4
Or see this region's nucleotide sequence