Experiment: WM_K30
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuE and yeaR are separated by 172 nucleotides yeaR and yoaG are separated by 3 nucleotides yoaG and yeaQ are separated by 146 nucleotides yeaQ and yoaJ are separated by 58 nucleotides yoaJ and dgcP are separated by 133 nucleotides
JDDGAC_01210: leuE - leucine efflux protein LeuE, at 206,876 to 207,514
leuE
JDDGAC_01215: yeaR - Uncharacterized protein YeaR, at 207,687 to 208,046
yeaR
JDDGAC_01220: yoaG - Protein YoaG, at 208,050 to 208,232
yoaG
JDDGAC_01225: yeaQ - UPF0410 protein YeaQ, at 208,379 to 208,627
yeaQ
JDDGAC_01230: yoaJ - protein YoaJ, at 208,686 to 208,760
yoaJ
JDDGAC_01235: dgcP - diguanylate cyclase DgcP, at 208,894 to 209,919
dgcP
Position (kb) 208
209 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 207.420 kb on + strand, within leuE at 207.420 kb on + strand, within leuE at 207.420 kb on + strand, within leuE at 207.421 kb on - strand, within leuE at 207.424 kb on + strand, within leuE at 207.964 kb on - strand, within yeaR at 208.065 kb on + strand at 208.067 kb on + strand at 208.181 kb on + strand, within yoaG at 208.195 kb on - strand, within yoaG at 208.235 kb on - strand at 208.474 kb on + strand, within yeaQ at 208.599 kb on + strand, within yeaQ at 208.676 kb on - strand at 208.865 kb on - strand at 208.889 kb on - strand at 208.956 kb on - strand at 208.979 kb on - strand at 209.042 kb on + strand, within dgcP at 209.063 kb on - strand, within dgcP at 209.081 kb on - strand, within dgcP at 209.202 kb on + strand, within dgcP at 209.242 kb on - strand, within dgcP at 209.486 kb on - strand, within dgcP at 209.626 kb on - strand, within dgcP
Per-strain Table
Position Strand Gene LocusTag Fraction WM_K30 remove 207,420 + leuE JDDGAC_01210 0.85 +1.1 207,420 + leuE JDDGAC_01210 0.85 -0.3 207,420 + leuE JDDGAC_01210 0.85 +0.4 207,421 - leuE JDDGAC_01210 0.85 -0.5 207,424 + leuE JDDGAC_01210 0.86 -2.8 207,964 - yeaR JDDGAC_01215 0.77 -0.7 208,065 + +0.7 208,067 + -0.2 208,181 + yoaG JDDGAC_01220 0.72 +0.6 208,195 - yoaG JDDGAC_01220 0.79 +0.1 208,235 - -0.6 208,474 + yeaQ JDDGAC_01225 0.38 -2.8 208,599 + yeaQ JDDGAC_01225 0.88 -1.6 208,676 - -0.8 208,865 - +0.0 208,889 - +0.8 208,956 - -0.4 208,979 - -1.7 209,042 + dgcP JDDGAC_01235 0.14 +2.0 209,063 - dgcP JDDGAC_01235 0.16 +1.2 209,081 - dgcP JDDGAC_01235 0.18 -0.6 209,202 + dgcP JDDGAC_01235 0.30 -0.8 209,242 - dgcP JDDGAC_01235 0.34 +0.3 209,486 - dgcP JDDGAC_01235 0.58 -0.3 209,626 - dgcP JDDGAC_01235 0.71 +0.3
Or see this region's nucleotide sequence