Experiment: R2A_PIPES no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00010 and LRK53_RS00015 are separated by 96 nucleotides LRK53_RS00015 and LRK53_RS00020 are separated by 95 nucleotides
LRK53_RS00010: LRK53_RS00010 - DNA polymerase III subunit beta, at 1,576 to 2,676
_RS00010
LRK53_RS00015: LRK53_RS00015 - DNA replication/repair protein RecF, at 2,773 to 3,852
_RS00015
LRK53_RS00020: LRK53_RS00020 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 3,948 to 6,380
_RS00020
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.815 kb on - strand at 2.815 kb on - strand at 3.173 kb on - strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.606 kb on + strand, within LRK53_RS00015 at 3.607 kb on - strand, within LRK53_RS00015 at 3.644 kb on - strand, within LRK53_RS00015 at 3.644 kb on - strand, within LRK53_RS00015 at 3.644 kb on - strand, within LRK53_RS00015 at 3.848 kb on + strand at 3.848 kb on + strand at 3.848 kb on + strand at 3.848 kb on + strand at 3.849 kb on - strand at 3.850 kb on + strand at 3.850 kb on + strand at 3.850 kb on + strand at 3.850 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES no stress control remove 2,814 + +0.1 2,814 + -0.9 2,814 + +2.7 2,814 + -0.9 2,814 + -1.2 2,814 + -2.4 2,815 - -0.2 2,815 - -0.9 3,173 - LRK53_RS00015 0.37 -0.5 3,354 + LRK53_RS00015 0.54 -0.9 3,354 + LRK53_RS00015 0.54 -0.0 3,354 + LRK53_RS00015 0.54 +1.1 3,354 + LRK53_RS00015 0.54 -0.9 3,354 + LRK53_RS00015 0.54 -0.9 3,354 + LRK53_RS00015 0.54 +0.1 3,354 + LRK53_RS00015 0.54 -0.5 3,354 + LRK53_RS00015 0.54 -0.5 3,354 + LRK53_RS00015 0.54 -2.6 3,354 + LRK53_RS00015 0.54 +0.5 3,355 - LRK53_RS00015 0.54 +0.1 3,355 - LRK53_RS00015 0.54 -0.5 3,355 - LRK53_RS00015 0.54 -0.9 3,355 - LRK53_RS00015 0.54 -1.7 3,355 - LRK53_RS00015 0.54 -1.2 3,355 - LRK53_RS00015 0.54 -0.5 3,358 + LRK53_RS00015 0.54 -1.5 3,358 + LRK53_RS00015 0.54 -1.7 3,358 + LRK53_RS00015 0.54 +2.1 3,358 + LRK53_RS00015 0.54 +1.1 3,358 + LRK53_RS00015 0.54 -0.5 3,358 + LRK53_RS00015 0.54 -0.5 3,359 - LRK53_RS00015 0.54 -0.5 3,359 - LRK53_RS00015 0.54 -1.2 3,359 - LRK53_RS00015 0.54 +1.1 3,359 - LRK53_RS00015 0.54 -1.9 3,359 - LRK53_RS00015 0.54 -0.5 3,516 + LRK53_RS00015 0.69 -0.1 3,516 + LRK53_RS00015 0.69 +1.7 3,516 + LRK53_RS00015 0.69 -2.2 3,516 + LRK53_RS00015 0.69 +1.1 3,516 + LRK53_RS00015 0.69 +0.4 3,516 + LRK53_RS00015 0.69 -2.1 3,517 - LRK53_RS00015 0.69 -2.9 3,517 - LRK53_RS00015 0.69 -0.5 3,517 - LRK53_RS00015 0.69 +0.1 3,517 - LRK53_RS00015 0.69 +0.1 3,517 - LRK53_RS00015 0.69 -0.9 3,517 - LRK53_RS00015 0.69 -1.2 3,517 - LRK53_RS00015 0.69 -1.7 3,517 - LRK53_RS00015 0.69 -1.5 3,517 - LRK53_RS00015 0.69 -1.2 3,517 - LRK53_RS00015 0.69 -1.5 3,606 + LRK53_RS00015 0.77 +1.1 3,607 - LRK53_RS00015 0.77 -1.2 3,644 - LRK53_RS00015 0.81 -0.5 3,644 - LRK53_RS00015 0.81 -0.2 3,644 - LRK53_RS00015 0.81 -0.5 3,848 + +0.1 3,848 + -1.9 3,848 + -2.5 3,848 + +1.1 3,849 - +0.1 3,850 + +0.4 3,850 + -0.7 3,850 + +0.1 3,850 + +1.1 3,854 + -1.2 3,854 + -0.7 3,854 + +1.1 3,854 + -0.9 3,854 + -1.5 3,854 + -1.2 3,854 + -0.9 3,854 + -1.5 3,854 + +0.1 3,854 + -1.1 3,854 + -0.5 3,854 + +1.7
Or see this region's nucleotide sequence