Experiment: R2A_PIPES no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18435 and LRK53_RS18440 are separated by 262 nucleotides LRK53_RS18440 and LRK53_RS18445 are separated by 127 nucleotides LRK53_RS18445 and LRK53_RS18450 are separated by 5 nucleotides LRK53_RS18450 and LRK53_RS18455 are separated by 30 nucleotides
LRK53_RS18435: LRK53_RS18435 - replicative DNA helicase, at 61,984 to 63,387
_RS18435
LRK53_RS18440: LRK53_RS18440 - hypothetical protein, at 63,650 to 64,222
_RS18440
LRK53_RS18445: LRK53_RS18445 - hypothetical protein, at 64,350 to 64,673
_RS18445
LRK53_RS18450: LRK53_RS18450 - hypothetical protein, at 64,679 to 64,990
_RS18450
LRK53_RS18455: LRK53_RS18455 - DUF3761 domain-containing protein, at 65,021 to 65,623
_RS18455
Position (kb) 64
65 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.461 kb on + strand at 63.461 kb on + strand at 63.461 kb on + strand at 63.461 kb on + strand at 63.462 kb on - strand at 63.493 kb on + strand at 63.523 kb on + strand at 63.523 kb on + strand at 63.596 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.678 kb on + strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.679 kb on - strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.680 kb on + strand at 63.681 kb on - strand at 63.681 kb on - strand at 63.681 kb on - strand at 63.681 kb on - strand at 63.908 kb on + strand, within LRK53_RS18440 at 63.908 kb on + strand, within LRK53_RS18440 at 63.908 kb on + strand, within LRK53_RS18440 at 63.908 kb on + strand, within LRK53_RS18440 at 63.909 kb on - strand, within LRK53_RS18440 at 63.909 kb on - strand, within LRK53_RS18440 at 63.909 kb on - strand, within LRK53_RS18440 at 63.909 kb on - strand, within LRK53_RS18440 at 63.920 kb on + strand, within LRK53_RS18440 at 63.920 kb on + strand, within LRK53_RS18440 at 63.921 kb on - strand, within LRK53_RS18440 at 63.921 kb on - strand, within LRK53_RS18440 at 64.058 kb on + strand, within LRK53_RS18440 at 64.094 kb on + strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.254 kb on - strand at 64.290 kb on - strand at 64.364 kb on - strand at 64.366 kb on + strand at 64.366 kb on + strand at 64.366 kb on + strand at 64.367 kb on - strand at 64.367 kb on - strand at 64.367 kb on - strand at 64.367 kb on - strand at 64.419 kb on + strand, within LRK53_RS18445 at 64.419 kb on + strand, within LRK53_RS18445 at 64.420 kb on - strand, within LRK53_RS18445 at 64.537 kb on - strand, within LRK53_RS18445 at 64.750 kb on + strand, within LRK53_RS18450 at 64.751 kb on - strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.895 kb on + strand, within LRK53_RS18450 at 64.895 kb on + strand, within LRK53_RS18450 at 64.896 kb on - strand, within LRK53_RS18450 at 64.896 kb on - strand, within LRK53_RS18450 at 65.020 kb on + strand at 65.020 kb on + strand at 65.020 kb on + strand at 65.020 kb on + strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.181 kb on - strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.644 kb on + strand at 65.644 kb on + strand at 65.644 kb on + strand at 65.644 kb on + strand at 65.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES no stress control remove 63,461 + -0.7 63,461 + -1.1 63,461 + +0.3 63,461 + +0.1 63,462 - -0.5 63,493 + +1.1 63,523 + +0.4 63,523 + -0.9 63,596 + -0.9 63,678 + -0.1 63,678 + -0.3 63,678 + +0.6 63,678 + +1.7 63,678 + +0.0 63,678 + +0.4 63,678 + -1.7 63,678 + -0.7 63,678 + -1.1 63,678 + +0.1 63,678 + +1.5 63,679 - -0.5 63,679 - -1.8 63,679 - +0.8 63,679 - -1.4 63,679 - +0.4 63,679 - +0.1 63,679 - -0.6 63,679 - -0.1 63,679 - -0.2 63,680 + -1.3 63,680 + +1.8 63,680 + +0.5 63,680 + -0.5 63,680 + -1.8 63,680 + -0.3 63,680 + -1.6 63,680 + -1.1 63,680 + -1.1 63,681 - -0.1 63,681 - -0.9 63,681 - -0.8 63,681 - -0.6 63,908 + LRK53_RS18440 0.45 -2.3 63,908 + LRK53_RS18440 0.45 +2.1 63,908 + LRK53_RS18440 0.45 -0.8 63,908 + LRK53_RS18440 0.45 +0.5 63,909 - LRK53_RS18440 0.45 -1.2 63,909 - LRK53_RS18440 0.45 -0.1 63,909 - LRK53_RS18440 0.45 -1.7 63,909 - LRK53_RS18440 0.45 -1.1 63,920 + LRK53_RS18440 0.47 -0.8 63,920 + LRK53_RS18440 0.47 -0.7 63,921 - LRK53_RS18440 0.47 -1.5 63,921 - LRK53_RS18440 0.47 +0.1 64,058 + LRK53_RS18440 0.71 -1.5 64,094 + LRK53_RS18440 0.77 -1.7 64,095 - LRK53_RS18440 0.78 -0.9 64,095 - LRK53_RS18440 0.78 -0.5 64,095 - LRK53_RS18440 0.78 +0.8 64,095 - LRK53_RS18440 0.78 -0.1 64,254 - -0.5 64,290 - +1.1 64,364 - -0.8 64,366 + -0.5 64,366 + +0.7 64,366 + -1.7 64,367 - +0.5 64,367 - +0.7 64,367 - -2.2 64,367 - -0.1 64,419 + LRK53_RS18445 0.21 -1.2 64,419 + LRK53_RS18445 0.21 -1.4 64,420 - LRK53_RS18445 0.22 +0.1 64,537 - LRK53_RS18445 0.58 -2.3 64,750 + LRK53_RS18450 0.23 -1.5 64,751 - LRK53_RS18450 0.23 -1.3 64,868 + LRK53_RS18450 0.61 -0.6 64,868 + LRK53_RS18450 0.61 -0.1 64,868 + LRK53_RS18450 0.61 -0.5 64,868 + LRK53_RS18450 0.61 -0.7 64,869 - LRK53_RS18450 0.61 -0.1 64,869 - LRK53_RS18450 0.61 -2.3 64,869 - LRK53_RS18450 0.61 +0.4 64,869 - LRK53_RS18450 0.61 +0.1 64,869 - LRK53_RS18450 0.61 -0.2 64,869 - LRK53_RS18450 0.61 -0.5 64,895 + LRK53_RS18450 0.69 -0.2 64,895 + LRK53_RS18450 0.69 -0.9 64,896 - LRK53_RS18450 0.70 -1.7 64,896 - LRK53_RS18450 0.70 +1.1 65,020 + +2.1 65,020 + +0.1 65,020 + -0.9 65,020 + -0.4 65,021 - -0.5 65,021 - -0.2 65,021 - -0.0 65,180 + LRK53_RS18455 0.26 -2.3 65,180 + LRK53_RS18455 0.26 -0.9 65,180 + LRK53_RS18455 0.26 -1.7 65,180 + LRK53_RS18455 0.26 -1.2 65,181 - LRK53_RS18455 0.27 -1.9 65,393 + LRK53_RS18455 0.62 -0.5 65,393 + LRK53_RS18455 0.62 +0.1 65,393 + LRK53_RS18455 0.62 -0.7 65,394 - LRK53_RS18455 0.62 -1.2 65,394 - LRK53_RS18455 0.62 -0.7 65,394 - LRK53_RS18455 0.62 -0.9 65,473 + LRK53_RS18455 0.75 -0.9 65,473 + LRK53_RS18455 0.75 -0.5 65,473 + LRK53_RS18455 0.75 -0.5 65,473 + LRK53_RS18455 0.75 -0.5 65,474 - LRK53_RS18455 0.75 -1.1 65,474 - LRK53_RS18455 0.75 +0.1 65,474 - LRK53_RS18455 0.75 -0.3 65,474 - LRK53_RS18455 0.75 -0.5 65,474 - LRK53_RS18455 0.75 +0.1 65,474 - LRK53_RS18455 0.75 +0.1 65,525 + LRK53_RS18455 0.84 -0.2 65,525 + LRK53_RS18455 0.84 -0.7 65,525 + LRK53_RS18455 0.84 -2.5 65,525 + LRK53_RS18455 0.84 -0.1 65,525 + LRK53_RS18455 0.84 -0.8 65,525 + LRK53_RS18455 0.84 -0.9 65,525 + LRK53_RS18455 0.84 -0.9 65,525 + LRK53_RS18455 0.84 -0.7 65,525 + LRK53_RS18455 0.84 -0.6 65,526 - LRK53_RS18455 0.84 -0.5 65,526 - LRK53_RS18455 0.84 +1.1 65,526 - LRK53_RS18455 0.84 -0.9 65,526 - LRK53_RS18455 0.84 -0.8 65,644 + -1.2 65,644 + +1.1 65,644 + -0.5 65,644 + -0.3 65,647 + -0.5
Or see this region's nucleotide sequence