Experiment: pH4.8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04210 and LRK53_RS04215 are separated by 57 nucleotides LRK53_RS04215 and LRK53_RS04220 are separated by 77 nucleotides LRK53_RS04220 and LRK53_RS04225 are separated by 126 nucleotides
LRK53_RS04210: LRK53_RS04210 - hypothetical protein, at 983,625 to 983,951
_RS04210
LRK53_RS04215: LRK53_RS04215 - trimeric intracellular cation channel family protein, at 984,009 to 984,635
_RS04215
LRK53_RS04220: LRK53_RS04220 - hypothetical protein, at 984,713 to 984,991
_RS04220
LRK53_RS04225: LRK53_RS04225 - DNA polymerase IV, at 985,118 to 986,212
_RS04225
Position (kb) 984
985 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 983.949 kb on + strand at 983.949 kb on + strand at 983.950 kb on - strand at 984.050 kb on + strand at 984.050 kb on + strand at 984.050 kb on + strand at 984.050 kb on + strand at 984.050 kb on + strand at 984.050 kb on + strand at 984.050 kb on + strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.051 kb on - strand at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.129 kb on + strand, within LRK53_RS04215 at 984.130 kb on - strand, within LRK53_RS04215 at 984.130 kb on - strand, within LRK53_RS04215 at 984.130 kb on - strand, within LRK53_RS04215 at 984.130 kb on - strand, within LRK53_RS04215 at 984.130 kb on - strand, within LRK53_RS04215 at 984.130 kb on - strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.131 kb on + strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.132 kb on - strand, within LRK53_RS04215 at 984.133 kb on - strand, within LRK53_RS04215 at 984.210 kb on + strand, within LRK53_RS04215 at 984.210 kb on + strand, within LRK53_RS04215 at 984.211 kb on - strand, within LRK53_RS04215 at 984.242 kb on + strand, within LRK53_RS04215 at 984.242 kb on + strand, within LRK53_RS04215 at 984.242 kb on + strand, within LRK53_RS04215 at 984.242 kb on + strand, within LRK53_RS04215 at 984.242 kb on + strand, within LRK53_RS04215 at 984.242 kb on + strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.243 kb on - strand, within LRK53_RS04215 at 984.280 kb on - strand, within LRK53_RS04215 at 984.327 kb on + strand, within LRK53_RS04215 at 984.327 kb on + strand, within LRK53_RS04215 at 984.328 kb on - strand, within LRK53_RS04215 at 984.328 kb on - strand, within LRK53_RS04215 at 984.328 kb on - strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.465 kb on + strand, within LRK53_RS04215 at 984.466 kb on - strand, within LRK53_RS04215 at 984.466 kb on - strand, within LRK53_RS04215 at 984.466 kb on - strand, within LRK53_RS04215 at 984.466 kb on - strand, within LRK53_RS04215 at 984.466 kb on - strand, within LRK53_RS04215 at 984.466 kb on - strand, within LRK53_RS04215 at 984.466 kb on - strand, within LRK53_RS04215 at 984.513 kb on + strand, within LRK53_RS04215 at 984.513 kb on + strand, within LRK53_RS04215 at 984.514 kb on - strand, within LRK53_RS04215 at 984.588 kb on + strand at 984.588 kb on + strand at 984.589 kb on - strand at 984.589 kb on - strand at 984.589 kb on - strand at 984.589 kb on - strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.710 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.721 kb on + strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.722 kb on - strand at 984.805 kb on + strand, within LRK53_RS04220 at 984.805 kb on + strand, within LRK53_RS04220 at 984.806 kb on - strand, within LRK53_RS04220 at 984.933 kb on + strand, within LRK53_RS04220 at 984.933 kb on + strand, within LRK53_RS04220 at 984.933 kb on + strand, within LRK53_RS04220 at 984.934 kb on - strand, within LRK53_RS04220 at 984.934 kb on - strand, within LRK53_RS04220 at 984.934 kb on - strand, within LRK53_RS04220 at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.996 kb on + strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 984.997 kb on - strand at 985.037 kb on - strand at 985.037 kb on - strand at 985.152 kb on + strand at 985.152 kb on + strand at 985.152 kb on + strand at 985.152 kb on + strand at 985.153 kb on - strand at 985.153 kb on - strand at 985.153 kb on - strand at 985.153 kb on - strand at 985.153 kb on - strand at 985.153 kb on - strand at 985.153 kb on - strand at 985.153 kb on - strand at 985.340 kb on + strand, within LRK53_RS04225 at 985.340 kb on + strand, within LRK53_RS04225 at 985.341 kb on - strand, within LRK53_RS04225 at 985.341 kb on - strand, within LRK53_RS04225 at 985.685 kb on + strand, within LRK53_RS04225 at 985.685 kb on + strand, within LRK53_RS04225 at 985.685 kb on + strand, within LRK53_RS04225 at 985.685 kb on + strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.686 kb on - strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.707 kb on + strand, within LRK53_RS04225 at 985.708 kb on - strand, within LRK53_RS04225 at 985.708 kb on - strand, within LRK53_RS04225 at 985.708 kb on - strand, within LRK53_RS04225 at 985.708 kb on - strand, within LRK53_RS04225 at 985.708 kb on - strand, within LRK53_RS04225 at 985.875 kb on + strand, within LRK53_RS04225 at 985.875 kb on + strand, within LRK53_RS04225 at 985.875 kb on + strand, within LRK53_RS04225 at 985.876 kb on - strand, within LRK53_RS04225 at 985.876 kb on - strand, within LRK53_RS04225 at 985.876 kb on - strand, within LRK53_RS04225 at 985.876 kb on - strand, within LRK53_RS04225 at 985.876 kb on - strand, within LRK53_RS04225 at 985.876 kb on - strand, within LRK53_RS04225 at 985.876 kb on - strand, within LRK53_RS04225 at 985.956 kb on + strand, within LRK53_RS04225 at 985.956 kb on + strand, within LRK53_RS04225 at 985.957 kb on - strand, within LRK53_RS04225 at 985.957 kb on - strand, within LRK53_RS04225 at 985.957 kb on - strand, within LRK53_RS04225
Per-strain Table
Position Strand Gene LocusTag Fraction pH4.8 remove 983,949 + -1.0 983,949 + -0.0 983,950 - -1.0 984,050 + -0.9 984,050 + -1.5 984,050 + -0.0 984,050 + +1.0 984,050 + -1.4 984,050 + -0.6 984,050 + +0.1 984,051 - -0.6 984,051 - +0.4 984,051 - -1.1 984,051 - -1.0 984,051 - -0.0 984,051 - -1.6 984,051 - -1.7 984,051 - -1.4 984,051 - -0.8 984,129 + LRK53_RS04215 0.19 -2.5 984,129 + LRK53_RS04215 0.19 -1.0 984,129 + LRK53_RS04215 0.19 -0.0 984,129 + LRK53_RS04215 0.19 +2.6 984,129 + LRK53_RS04215 0.19 -1.2 984,129 + LRK53_RS04215 0.19 +0.2 984,129 + LRK53_RS04215 0.19 +0.7 984,129 + LRK53_RS04215 0.19 -1.0 984,129 + LRK53_RS04215 0.19 -2.5 984,129 + LRK53_RS04215 0.19 +0.2 984,129 + LRK53_RS04215 0.19 -0.0 984,130 - LRK53_RS04215 0.19 +0.6 984,130 - LRK53_RS04215 0.19 -0.6 984,130 - LRK53_RS04215 0.19 -1.8 984,130 - LRK53_RS04215 0.19 -2.6 984,130 - LRK53_RS04215 0.19 -1.0 984,130 - LRK53_RS04215 0.19 +1.0 984,131 + LRK53_RS04215 0.19 -0.7 984,131 + LRK53_RS04215 0.19 -1.4 984,131 + LRK53_RS04215 0.19 -0.9 984,131 + LRK53_RS04215 0.19 -0.0 984,131 + LRK53_RS04215 0.19 -2.5 984,131 + LRK53_RS04215 0.19 +1.0 984,131 + LRK53_RS04215 0.19 -2.1 984,131 + LRK53_RS04215 0.19 +0.6 984,131 + LRK53_RS04215 0.19 -1.3 984,131 + LRK53_RS04215 0.19 -0.5 984,131 + LRK53_RS04215 0.19 +0.1 984,131 + LRK53_RS04215 0.19 +0.3 984,131 + LRK53_RS04215 0.19 -0.6 984,132 - LRK53_RS04215 0.20 -0.2 984,132 - LRK53_RS04215 0.20 +0.4 984,132 - LRK53_RS04215 0.20 -0.0 984,132 - LRK53_RS04215 0.20 +1.0 984,132 - LRK53_RS04215 0.20 -0.0 984,132 - LRK53_RS04215 0.20 -1.5 984,132 - LRK53_RS04215 0.20 -0.0 984,132 - LRK53_RS04215 0.20 -0.4 984,132 - LRK53_RS04215 0.20 -1.7 984,132 - LRK53_RS04215 0.20 +2.0 984,132 - LRK53_RS04215 0.20 +1.0 984,132 - LRK53_RS04215 0.20 -1.6 984,132 - LRK53_RS04215 0.20 -1.7 984,132 - LRK53_RS04215 0.20 +2.0 984,133 - LRK53_RS04215 0.20 +0.4 984,210 + LRK53_RS04215 0.32 -0.0 984,210 + LRK53_RS04215 0.32 +1.0 984,211 - LRK53_RS04215 0.32 -0.0 984,242 + LRK53_RS04215 0.37 -0.6 984,242 + LRK53_RS04215 0.37 -0.6 984,242 + LRK53_RS04215 0.37 +1.0 984,242 + LRK53_RS04215 0.37 +2.0 984,242 + LRK53_RS04215 0.37 -1.4 984,242 + LRK53_RS04215 0.37 -0.0 984,243 - LRK53_RS04215 0.37 -1.0 984,243 - LRK53_RS04215 0.37 -0.6 984,243 - LRK53_RS04215 0.37 +1.0 984,243 - LRK53_RS04215 0.37 -1.4 984,243 - LRK53_RS04215 0.37 -1.0 984,243 - LRK53_RS04215 0.37 -0.6 984,243 - LRK53_RS04215 0.37 -0.6 984,243 - LRK53_RS04215 0.37 -0.3 984,280 - LRK53_RS04215 0.43 +0.1 984,327 + LRK53_RS04215 0.51 -2.7 984,327 + LRK53_RS04215 0.51 -2.2 984,328 - LRK53_RS04215 0.51 -0.5 984,328 - LRK53_RS04215 0.51 -0.8 984,328 - LRK53_RS04215 0.51 -1.6 984,465 + LRK53_RS04215 0.73 -2.3 984,465 + LRK53_RS04215 0.73 -0.7 984,465 + LRK53_RS04215 0.73 -0.4 984,465 + LRK53_RS04215 0.73 -1.2 984,465 + LRK53_RS04215 0.73 -0.0 984,465 + LRK53_RS04215 0.73 -1.0 984,465 + LRK53_RS04215 0.73 -0.4 984,465 + LRK53_RS04215 0.73 -1.0 984,465 + LRK53_RS04215 0.73 -0.5 984,465 + LRK53_RS04215 0.73 -0.8 984,465 + LRK53_RS04215 0.73 -0.5 984,465 + LRK53_RS04215 0.73 -2.0 984,465 + LRK53_RS04215 0.73 -0.2 984,465 + LRK53_RS04215 0.73 -1.6 984,465 + LRK53_RS04215 0.73 +1.0 984,465 + LRK53_RS04215 0.73 +0.8 984,465 + LRK53_RS04215 0.73 +2.0 984,466 - LRK53_RS04215 0.73 +0.4 984,466 - LRK53_RS04215 0.73 -0.0 984,466 - LRK53_RS04215 0.73 +2.0 984,466 - LRK53_RS04215 0.73 -0.9 984,466 - LRK53_RS04215 0.73 -0.8 984,466 - LRK53_RS04215 0.73 -0.0 984,466 - LRK53_RS04215 0.73 -1.0 984,513 + LRK53_RS04215 0.80 -2.6 984,513 + LRK53_RS04215 0.80 -0.0 984,514 - LRK53_RS04215 0.81 +0.2 984,588 + +0.4 984,588 + -0.6 984,589 - -1.4 984,589 - -1.4 984,589 - -0.0 984,589 - -0.1 984,710 + -1.0 984,710 + +1.0 984,710 + +1.6 984,710 + +0.2 984,710 + +0.1 984,710 + -0.5 984,710 + -1.1 984,710 + -0.6 984,710 + +0.4 984,710 + -2.0 984,710 + +0.4 984,710 + +2.8 984,710 + -0.8 984,721 + +1.0 984,721 + -0.8 984,721 + -0.0 984,721 + -0.8 984,721 + -1.0 984,721 + +2.6 984,721 + -0.6 984,721 + +1.0 984,721 + -1.5 984,721 + -1.3 984,721 + -0.5 984,721 + -1.4 984,721 + -1.0 984,722 - +1.0 984,722 - -0.9 984,722 - +2.0 984,722 - -1.6 984,722 - -1.0 984,722 - +1.0 984,722 - -1.4 984,722 - -1.4 984,722 - +0.1 984,722 - +0.2 984,805 + LRK53_RS04220 0.33 -1.0 984,805 + LRK53_RS04220 0.33 -0.7 984,806 - LRK53_RS04220 0.33 -0.6 984,933 + LRK53_RS04220 0.79 -0.9 984,933 + LRK53_RS04220 0.79 -1.0 984,933 + LRK53_RS04220 0.79 -0.0 984,934 - LRK53_RS04220 0.79 -1.3 984,934 - LRK53_RS04220 0.79 -2.2 984,934 - LRK53_RS04220 0.79 -1.6 984,996 + -1.0 984,996 + +0.4 984,996 + -0.9 984,996 + -1.6 984,996 + -0.5 984,996 + -1.0 984,996 + -0.0 984,996 + -0.9 984,996 + +2.0 984,996 + -1.3 984,996 + -0.6 984,996 + -1.0 984,996 + -0.9 984,997 - +1.0 984,997 - -0.7 984,997 - +1.0 984,997 - -0.4 984,997 - -1.6 984,997 - -0.8 984,997 - +0.4 984,997 - -0.0 984,997 - -0.8 984,997 - -0.7 984,997 - -0.0 984,997 - -0.6 984,997 - +0.4 984,997 - +1.0 984,997 - +0.2 984,997 - -0.0 984,997 - +0.4 984,997 - -0.0 984,997 - -1.4 985,037 - +1.0 985,037 - -1.5 985,152 + -1.6 985,152 + -0.6 985,152 + +0.4 985,152 + -1.4 985,153 - -0.8 985,153 - -0.0 985,153 - +1.0 985,153 - -0.0 985,153 - +0.4 985,153 - -0.2 985,153 - -1.4 985,153 - -0.6 985,340 + LRK53_RS04225 0.20 -1.1 985,340 + LRK53_RS04225 0.20 +2.6 985,341 - LRK53_RS04225 0.20 +1.0 985,341 - LRK53_RS04225 0.20 -2.6 985,685 + LRK53_RS04225 0.52 +1.6 985,685 + LRK53_RS04225 0.52 -1.4 985,685 + LRK53_RS04225 0.52 -0.0 985,685 + LRK53_RS04225 0.52 -1.3 985,686 - LRK53_RS04225 0.52 -0.8 985,686 - LRK53_RS04225 0.52 -2.0 985,686 - LRK53_RS04225 0.52 -1.2 985,686 - LRK53_RS04225 0.52 -0.6 985,686 - LRK53_RS04225 0.52 -0.4 985,686 - LRK53_RS04225 0.52 +1.0 985,686 - LRK53_RS04225 0.52 -1.0 985,686 - LRK53_RS04225 0.52 -1.0 985,686 - LRK53_RS04225 0.52 -1.3 985,686 - LRK53_RS04225 0.52 -0.8 985,686 - LRK53_RS04225 0.52 -3.1 985,686 - LRK53_RS04225 0.52 +1.7 985,707 + LRK53_RS04225 0.54 +0.8 985,707 + LRK53_RS04225 0.54 -2.0 985,707 + LRK53_RS04225 0.54 -0.0 985,707 + LRK53_RS04225 0.54 -0.6 985,707 + LRK53_RS04225 0.54 -1.0 985,707 + LRK53_RS04225 0.54 -2.0 985,707 + LRK53_RS04225 0.54 -0.3 985,707 + LRK53_RS04225 0.54 -0.0 985,707 + LRK53_RS04225 0.54 -1.3 985,708 - LRK53_RS04225 0.54 -1.0 985,708 - LRK53_RS04225 0.54 -0.8 985,708 - LRK53_RS04225 0.54 -1.0 985,708 - LRK53_RS04225 0.54 +1.6 985,708 - LRK53_RS04225 0.54 +1.4 985,875 + LRK53_RS04225 0.69 -0.6 985,875 + LRK53_RS04225 0.69 -0.4 985,875 + LRK53_RS04225 0.69 -2.0 985,876 - LRK53_RS04225 0.69 +0.1 985,876 - LRK53_RS04225 0.69 +0.2 985,876 - LRK53_RS04225 0.69 -0.4 985,876 - LRK53_RS04225 0.69 -0.3 985,876 - LRK53_RS04225 0.69 -0.3 985,876 - LRK53_RS04225 0.69 +0.4 985,876 - LRK53_RS04225 0.69 -0.0 985,956 + LRK53_RS04225 0.77 -1.8 985,956 + LRK53_RS04225 0.77 -1.2 985,957 - LRK53_RS04225 0.77 +0.4 985,957 - LRK53_RS04225 0.77 -0.0 985,957 - LRK53_RS04225 0.77 -0.6
Or see this region's nucleotide sequence