Experiment: pH4.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08470 and LRK53_RS08475 overlap by 4 nucleotides LRK53_RS08475 and LRK53_RS08480 are separated by 142 nucleotides LRK53_RS08480 and LRK53_RS08485 are separated by 157 nucleotides
LRK53_RS08470: LRK53_RS08470 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,903,095 to 1,904,162
_RS08470
LRK53_RS08475: LRK53_RS08475 - imidazole glycerol phosphate synthase subunit HisH, at 1,904,159 to 1,904,752
_RS08475
LRK53_RS08480: LRK53_RS08480 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 1,904,895 to 1,905,626
_RS08480
LRK53_RS08485: LRK53_RS08485 - imidazole glycerol phosphate synthase subunit HisF, at 1,905,784 to 1,906,560
_RS08485
Position (kb)
1904
1905
1906 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1903.926 kb on + strand, within LRK53_RS08470 at 1903.926 kb on + strand, within LRK53_RS08470 at 1903.926 kb on + strand, within LRK53_RS08470 at 1903.927 kb on - strand, within LRK53_RS08470 at 1903.927 kb on - strand, within LRK53_RS08470 at 1904.100 kb on - strand at 1904.100 kb on - strand at 1904.213 kb on + strand at 1904.214 kb on - strand at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.579 kb on + strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.580 kb on - strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.594 kb on + strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.595 kb on - strand, within LRK53_RS08475 at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.748 kb on + strand at 1904.749 kb on - strand at 1904.749 kb on - strand at 1904.749 kb on - strand at 1904.749 kb on - strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.754 kb on + strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.755 kb on - strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.822 kb on + strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.823 kb on - strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.901 kb on + strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.902 kb on - strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.911 kb on + strand at 1904.912 kb on - strand at 1904.967 kb on + strand at 1904.967 kb on + strand at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.985 kb on + strand, within LRK53_RS08480 at 1904.986 kb on - strand, within LRK53_RS08480 at 1904.986 kb on - strand, within LRK53_RS08480 at 1904.986 kb on - strand, within LRK53_RS08480 at 1905.019 kb on - strand, within LRK53_RS08480 at 1905.019 kb on - strand, within LRK53_RS08480 at 1905.148 kb on + strand, within LRK53_RS08480 at 1905.258 kb on + strand, within LRK53_RS08480 at 1905.258 kb on + strand, within LRK53_RS08480 at 1905.258 kb on + strand, within LRK53_RS08480 at 1905.259 kb on - strand, within LRK53_RS08480 at 1905.259 kb on - strand, within LRK53_RS08480 at 1905.378 kb on + strand, within LRK53_RS08480 at 1905.379 kb on - strand, within LRK53_RS08480 at 1905.622 kb on + strand at 1905.622 kb on + strand at 1905.622 kb on + strand at 1905.623 kb on - strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.845 kb on + strand at 1905.846 kb on - strand at 1905.931 kb on + strand, within LRK53_RS08485 at 1905.931 kb on + strand, within LRK53_RS08485 at 1905.931 kb on + strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1905.932 kb on - strand, within LRK53_RS08485 at 1906.213 kb on + strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.214 kb on - strand, within LRK53_RS08485 at 1906.342 kb on + strand, within LRK53_RS08485 at 1906.502 kb on + strand at 1906.502 kb on + strand at 1906.502 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.519 kb on + strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.520 kb on - strand at 1906.545 kb on - strand at 1906.551 kb on + strand at 1906.551 kb on + strand at 1906.551 kb on + strand at 1906.551 kb on + strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.552 kb on - strand at 1906.558 kb on + strand at 1906.559 kb on - strand at 1906.559 kb on - strand at 1906.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pH4.5 remove 1,903,926 + LRK53_RS08470 0.78 +1.0 1,903,926 + LRK53_RS08470 0.78 +0.3 1,903,926 + LRK53_RS08470 0.78 -1.9 1,903,927 - LRK53_RS08470 0.78 -0.6 1,903,927 - LRK53_RS08470 0.78 +1.0 1,904,100 - -1.2 1,904,100 - -0.4 1,904,213 + +1.0 1,904,214 - +0.5 1,904,579 + LRK53_RS08475 0.71 -0.8 1,904,579 + LRK53_RS08475 0.71 -1.1 1,904,579 + LRK53_RS08475 0.71 +0.4 1,904,579 + LRK53_RS08475 0.71 -1.5 1,904,579 + LRK53_RS08475 0.71 -1.3 1,904,580 - LRK53_RS08475 0.71 -0.6 1,904,580 - LRK53_RS08475 0.71 -1.5 1,904,580 - LRK53_RS08475 0.71 -0.6 1,904,580 - LRK53_RS08475 0.71 -0.4 1,904,594 + LRK53_RS08475 0.73 +0.6 1,904,594 + LRK53_RS08475 0.73 -0.4 1,904,594 + LRK53_RS08475 0.73 +1.6 1,904,594 + LRK53_RS08475 0.73 +1.0 1,904,594 + LRK53_RS08475 0.73 +0.2 1,904,594 + LRK53_RS08475 0.73 -0.1 1,904,594 + LRK53_RS08475 0.73 +2.0 1,904,594 + LRK53_RS08475 0.73 -1.0 1,904,594 + LRK53_RS08475 0.73 -0.0 1,904,594 + LRK53_RS08475 0.73 +0.8 1,904,594 + LRK53_RS08475 0.73 -0.2 1,904,595 - LRK53_RS08475 0.73 -0.1 1,904,595 - LRK53_RS08475 0.73 -0.0 1,904,595 - LRK53_RS08475 0.73 -0.8 1,904,595 - LRK53_RS08475 0.73 -0.0 1,904,595 - LRK53_RS08475 0.73 +0.6 1,904,595 - LRK53_RS08475 0.73 +0.2 1,904,595 - LRK53_RS08475 0.73 -0.3 1,904,595 - LRK53_RS08475 0.73 -1.0 1,904,595 - LRK53_RS08475 0.73 +1.4 1,904,748 + +0.6 1,904,748 + -0.6 1,904,748 + -1.2 1,904,748 + -1.3 1,904,748 + -0.2 1,904,748 + -0.3 1,904,748 + -1.3 1,904,748 + -0.0 1,904,749 - -0.7 1,904,749 - -0.8 1,904,749 - -0.0 1,904,749 - -0.6 1,904,754 + -0.0 1,904,754 + +0.3 1,904,754 + -2.3 1,904,754 + -0.6 1,904,754 + -1.3 1,904,754 + -1.7 1,904,754 + -0.7 1,904,754 + -0.4 1,904,754 + -2.7 1,904,754 + +0.6 1,904,754 + -0.0 1,904,754 + +0.3 1,904,754 + +0.8 1,904,754 + -1.6 1,904,754 + -1.2 1,904,754 + -0.6 1,904,755 - -0.3 1,904,755 - +0.4 1,904,755 - -0.0 1,904,755 - -0.8 1,904,755 - -0.1 1,904,755 - -0.0 1,904,755 - -0.5 1,904,755 - -0.1 1,904,755 - -1.0 1,904,755 - -0.3 1,904,755 - -0.8 1,904,755 - -0.7 1,904,755 - +0.4 1,904,755 - -2.8 1,904,755 - -0.0 1,904,755 - -2.5 1,904,755 - -1.0 1,904,755 - +2.6 1,904,755 - -1.1 1,904,755 - -0.6 1,904,822 + -0.7 1,904,822 + +1.0 1,904,822 + -0.0 1,904,822 + -2.2 1,904,822 + +1.0 1,904,822 + +0.7 1,904,822 + -0.6 1,904,823 - -0.7 1,904,823 - -0.0 1,904,823 - -0.3 1,904,823 - +0.3 1,904,823 - +1.0 1,904,901 + -0.0 1,904,901 + -0.2 1,904,901 + -1.4 1,904,901 + -0.6 1,904,901 + -1.3 1,904,901 + -0.5 1,904,901 + -1.3 1,904,902 - -0.7 1,904,902 - -0.6 1,904,902 - -1.6 1,904,902 - +0.1 1,904,902 - +2.0 1,904,902 - -0.4 1,904,902 - -0.0 1,904,902 - +1.0 1,904,902 - +0.7 1,904,902 - -1.6 1,904,911 + -0.5 1,904,911 + -1.3 1,904,911 + +0.4 1,904,911 + -0.6 1,904,911 + +0.6 1,904,912 - -0.6 1,904,967 + +1.0 1,904,967 + +0.6 1,904,985 + LRK53_RS08480 0.12 -0.8 1,904,985 + LRK53_RS08480 0.12 -0.2 1,904,985 + LRK53_RS08480 0.12 -0.6 1,904,985 + LRK53_RS08480 0.12 -0.0 1,904,985 + LRK53_RS08480 0.12 -0.6 1,904,986 - LRK53_RS08480 0.12 -1.3 1,904,986 - LRK53_RS08480 0.12 -1.8 1,904,986 - LRK53_RS08480 0.12 -1.3 1,905,019 - LRK53_RS08480 0.17 -0.0 1,905,019 - LRK53_RS08480 0.17 +0.6 1,905,148 + LRK53_RS08480 0.35 -1.1 1,905,258 + LRK53_RS08480 0.50 -1.3 1,905,258 + LRK53_RS08480 0.50 -1.0 1,905,258 + LRK53_RS08480 0.50 -2.7 1,905,259 - LRK53_RS08480 0.50 -1.5 1,905,259 - LRK53_RS08480 0.50 +1.0 1,905,378 + LRK53_RS08480 0.66 -1.3 1,905,379 - LRK53_RS08480 0.66 -1.6 1,905,622 + -0.1 1,905,622 + +1.0 1,905,622 + -0.7 1,905,623 - +1.0 1,905,845 + -0.6 1,905,845 + -0.1 1,905,845 + -0.2 1,905,845 + -0.4 1,905,845 + +0.6 1,905,845 + +0.2 1,905,845 + -1.0 1,905,845 + -1.0 1,905,845 + -1.0 1,905,845 + -0.8 1,905,845 + -2.7 1,905,846 - -0.9 1,905,931 + LRK53_RS08485 0.19 -0.2 1,905,931 + LRK53_RS08485 0.19 -0.5 1,905,931 + LRK53_RS08485 0.19 -0.0 1,905,932 - LRK53_RS08485 0.19 +1.0 1,905,932 - LRK53_RS08485 0.19 -1.0 1,905,932 - LRK53_RS08485 0.19 -1.6 1,905,932 - LRK53_RS08485 0.19 -1.0 1,906,213 + LRK53_RS08485 0.55 +0.1 1,906,214 - LRK53_RS08485 0.55 -1.3 1,906,214 - LRK53_RS08485 0.55 -0.8 1,906,214 - LRK53_RS08485 0.55 -1.3 1,906,214 - LRK53_RS08485 0.55 -0.9 1,906,342 + LRK53_RS08485 0.72 -0.0 1,906,502 + -0.6 1,906,502 + -0.6 1,906,502 + -0.8 1,906,519 + -0.0 1,906,519 + -0.2 1,906,519 + +0.7 1,906,519 + -0.0 1,906,519 + +0.2 1,906,520 - -0.4 1,906,520 - -1.6 1,906,520 - -0.0 1,906,520 - -0.7 1,906,520 - -0.8 1,906,545 - -0.5 1,906,551 + +0.4 1,906,551 + +0.7 1,906,551 + +2.0 1,906,551 + -1.8 1,906,552 - -1.2 1,906,552 - +0.7 1,906,552 - -0.0 1,906,552 - +0.4 1,906,552 - +2.0 1,906,552 - -1.5 1,906,558 + -1.0 1,906,559 - -0.9 1,906,559 - -0.6 1,906,559 - -0.9
Or see this region's nucleotide sequence