Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18440 and LRK53_RS18445 are separated by 127 nucleotides LRK53_RS18445 and LRK53_RS18450 are separated by 5 nucleotides LRK53_RS18450 and LRK53_RS18455 are separated by 30 nucleotides LRK53_RS18455 and LRK53_RS18460 are separated by 62 nucleotides
LRK53_RS18440: LRK53_RS18440 - hypothetical protein, at 63,650 to 64,222
_RS18440
LRK53_RS18445: LRK53_RS18445 - hypothetical protein, at 64,350 to 64,673
_RS18445
LRK53_RS18450: LRK53_RS18450 - hypothetical protein, at 64,679 to 64,990
_RS18450
LRK53_RS18455: LRK53_RS18455 - DUF3761 domain-containing protein, at 65,021 to 65,623
_RS18455
LRK53_RS18460: LRK53_RS18460 - hypothetical protein, at 65,686 to 66,612
_RS18460
Position (kb)
65
66 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 64.058 kb on + strand, within LRK53_RS18440 at 64.094 kb on + strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.095 kb on - strand, within LRK53_RS18440 at 64.254 kb on - strand at 64.290 kb on - strand at 64.364 kb on - strand at 64.366 kb on + strand at 64.366 kb on + strand at 64.366 kb on + strand at 64.367 kb on - strand at 64.367 kb on - strand at 64.367 kb on - strand at 64.367 kb on - strand at 64.419 kb on + strand, within LRK53_RS18445 at 64.419 kb on + strand, within LRK53_RS18445 at 64.420 kb on - strand, within LRK53_RS18445 at 64.537 kb on - strand, within LRK53_RS18445 at 64.620 kb on + strand, within LRK53_RS18445 at 64.750 kb on + strand, within LRK53_RS18450 at 64.751 kb on - strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.895 kb on + strand, within LRK53_RS18450 at 64.895 kb on + strand, within LRK53_RS18450 at 64.896 kb on - strand, within LRK53_RS18450 at 64.896 kb on - strand, within LRK53_RS18450 at 65.020 kb on + strand at 65.020 kb on + strand at 65.020 kb on + strand at 65.020 kb on + strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.181 kb on - strand, within LRK53_RS18455 at 65.181 kb on - strand, within LRK53_RS18455 at 65.181 kb on - strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.644 kb on + strand at 65.644 kb on + strand at 65.644 kb on + strand at 65.644 kb on + strand at 65.647 kb on + strand at 65.647 kb on + strand at 65.712 kb on - strand at 65.712 kb on - strand at 65.764 kb on + strand at 65.764 kb on + strand at 65.764 kb on + strand at 65.764 kb on + strand at 65.764 kb on + strand at 65.765 kb on - strand at 65.765 kb on - strand at 65.765 kb on - strand at 65.765 kb on - strand at 65.765 kb on - strand at 66.184 kb on + strand, within LRK53_RS18460 at 66.184 kb on + strand, within LRK53_RS18460 at 66.184 kb on + strand, within LRK53_RS18460 at 66.185 kb on - strand, within LRK53_RS18460 at 66.435 kb on - strand, within LRK53_RS18460 at 66.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 64,058 + LRK53_RS18440 0.71 +0.3 64,094 + LRK53_RS18440 0.77 +0.4 64,095 - LRK53_RS18440 0.78 +0.2 64,095 - LRK53_RS18440 0.78 +0.2 64,095 - LRK53_RS18440 0.78 +0.2 64,095 - LRK53_RS18440 0.78 +0.1 64,254 - -2.3 64,290 - +0.9 64,364 - -0.9 64,366 + -0.7 64,366 + -0.2 64,366 + -0.9 64,367 - -0.7 64,367 - +0.7 64,367 - -0.4 64,367 - -1.3 64,419 + LRK53_RS18445 0.21 -0.4 64,419 + LRK53_RS18445 0.21 +0.3 64,420 - LRK53_RS18445 0.22 +0.5 64,537 - LRK53_RS18445 0.58 -0.5 64,620 + LRK53_RS18445 0.83 -0.7 64,750 + LRK53_RS18450 0.23 -0.1 64,751 - LRK53_RS18450 0.23 -0.6 64,868 + LRK53_RS18450 0.61 -1.2 64,868 + LRK53_RS18450 0.61 -0.3 64,868 + LRK53_RS18450 0.61 -1.1 64,868 + LRK53_RS18450 0.61 +1.3 64,868 + LRK53_RS18450 0.61 -1.9 64,869 - LRK53_RS18450 0.61 -0.3 64,869 - LRK53_RS18450 0.61 -0.5 64,869 - LRK53_RS18450 0.61 -0.4 64,869 - LRK53_RS18450 0.61 -0.1 64,869 - LRK53_RS18450 0.61 -0.8 64,869 - LRK53_RS18450 0.61 -1.3 64,895 + LRK53_RS18450 0.69 -0.5 64,895 + LRK53_RS18450 0.69 -0.4 64,896 - LRK53_RS18450 0.70 -0.9 64,896 - LRK53_RS18450 0.70 +0.9 65,020 + +1.9 65,020 + -1.1 65,020 + -0.1 65,020 + -1.4 65,021 - +0.9 65,021 - +1.5 65,021 - -0.5 65,021 - -0.4 65,021 - -1.0 65,180 + LRK53_RS18455 0.26 -1.0 65,180 + LRK53_RS18455 0.26 -0.1 65,180 + LRK53_RS18455 0.26 -1.9 65,180 + LRK53_RS18455 0.26 -2.4 65,180 + LRK53_RS18455 0.26 -0.1 65,181 - LRK53_RS18455 0.27 +0.3 65,181 - LRK53_RS18455 0.27 -0.1 65,181 - LRK53_RS18455 0.27 +0.5 65,393 + LRK53_RS18455 0.62 -0.7 65,393 + LRK53_RS18455 0.62 -0.7 65,393 + LRK53_RS18455 0.62 +0.9 65,393 + LRK53_RS18455 0.62 -0.3 65,394 - LRK53_RS18455 0.62 +0.2 65,394 - LRK53_RS18455 0.62 -2.5 65,394 - LRK53_RS18455 0.62 -2.1 65,473 + LRK53_RS18455 0.75 +0.9 65,473 + LRK53_RS18455 0.75 -1.5 65,473 + LRK53_RS18455 0.75 -0.1 65,473 + LRK53_RS18455 0.75 -0.1 65,473 + LRK53_RS18455 0.75 +0.9 65,473 + LRK53_RS18455 0.75 -0.2 65,474 - LRK53_RS18455 0.75 -1.1 65,474 - LRK53_RS18455 0.75 -0.9 65,474 - LRK53_RS18455 0.75 -0.4 65,474 - LRK53_RS18455 0.75 -0.7 65,474 - LRK53_RS18455 0.75 -0.5 65,474 - LRK53_RS18455 0.75 -0.4 65,474 - LRK53_RS18455 0.75 +0.9 65,474 - LRK53_RS18455 0.75 -1.3 65,525 + LRK53_RS18455 0.84 -1.4 65,525 + LRK53_RS18455 0.84 -1.5 65,525 + LRK53_RS18455 0.84 -1.7 65,525 + LRK53_RS18455 0.84 -0.3 65,525 + LRK53_RS18455 0.84 -0.1 65,525 + LRK53_RS18455 0.84 -0.6 65,525 + LRK53_RS18455 0.84 -1.0 65,525 + LRK53_RS18455 0.84 -1.1 65,525 + LRK53_RS18455 0.84 -0.0 65,525 + LRK53_RS18455 0.84 -0.0 65,526 - LRK53_RS18455 0.84 -1.7 65,526 - LRK53_RS18455 0.84 +1.9 65,526 - LRK53_RS18455 0.84 -0.1 65,526 - LRK53_RS18455 0.84 -1.0 65,644 + +0.9 65,644 + +0.9 65,644 + -1.2 65,644 + -1.1 65,647 + +0.7 65,647 + -1.4 65,712 - +0.5 65,712 - -1.4 65,764 + -1.7 65,764 + -0.7 65,764 + -0.8 65,764 + -0.1 65,764 + -1.1 65,765 - +0.7 65,765 - +0.2 65,765 - -1.1 65,765 - -0.1 65,765 - +1.6 66,184 + LRK53_RS18460 0.54 -1.3 66,184 + LRK53_RS18460 0.54 +0.0 66,184 + LRK53_RS18460 0.54 +0.5 66,185 - LRK53_RS18460 0.54 -0.1 66,435 - LRK53_RS18460 0.81 -0.7 66,572 + -1.3
Or see this region's nucleotide sequence