Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS12640 and LRK53_RS12645 are separated by 163 nucleotides LRK53_RS12645 and LRK53_RS12650 are separated by 25 nucleotides LRK53_RS12650 and LRK53_RS12655 are separated by 158 nucleotides
LRK53_RS12640: LRK53_RS12640 - cell wall hydrolase, at 2,798,392 to 2,798,826
_RS12640
LRK53_RS12645: LRK53_RS12645 - glycine zipper 2TM domain-containing protein, at 2,798,990 to 2,799,490
_RS12645
LRK53_RS12650: LRK53_RS12650 - glycine zipper 2TM domain-containing protein, at 2,799,516 to 2,800,097
_RS12650
LRK53_RS12655: LRK53_RS12655 - TatD family hydrolase, at 2,800,256 to 2,801,041
_RS12655
Position (kb)
2799
2800
2801 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2798.717 kb on + strand, within LRK53_RS12640 at 2798.717 kb on + strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.812 kb on + strand at 2798.813 kb on - strand at 2798.813 kb on - strand at 2798.987 kb on + strand at 2798.987 kb on + strand at 2798.988 kb on - strand at 2798.988 kb on - strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.027 kb on + strand at 2799.027 kb on + strand at 2799.027 kb on + strand at 2799.027 kb on + strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.076 kb on - strand, within LRK53_RS12645 at 2799.076 kb on - strand, within LRK53_RS12645 at 2799.294 kb on + strand, within LRK53_RS12645 at 2799.294 kb on + strand, within LRK53_RS12645 at 2799.294 kb on + strand, within LRK53_RS12645 at 2799.379 kb on - strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.408 kb on + strand, within LRK53_RS12645 at 2799.409 kb on - strand, within LRK53_RS12645 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.617 kb on - strand, within LRK53_RS12650 at 2799.628 kb on + strand, within LRK53_RS12650 at 2799.628 kb on + strand, within LRK53_RS12650 at 2799.925 kb on - strand, within LRK53_RS12650 at 2799.925 kb on - strand, within LRK53_RS12650 at 2799.925 kb on - strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.952 kb on + strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2799.953 kb on - strand, within LRK53_RS12650 at 2800.051 kb on - strand at 2800.074 kb on + strand at 2800.074 kb on + strand at 2800.074 kb on + strand at 2800.074 kb on + strand at 2800.074 kb on + strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.075 kb on - strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.161 kb on + strand at 2800.162 kb on - strand at 2800.162 kb on - strand at 2800.162 kb on - strand at 2800.162 kb on - strand at 2800.162 kb on - strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.203 kb on + strand at 2800.204 kb on - strand at 2800.204 kb on - strand at 2800.217 kb on + strand at 2800.217 kb on + strand at 2800.218 kb on - strand at 2800.218 kb on - strand at 2800.218 kb on - strand at 2800.222 kb on + strand at 2800.222 kb on + strand at 2800.222 kb on + strand at 2800.223 kb on - strand at 2800.223 kb on - strand at 2800.457 kb on + strand, within LRK53_RS12655 at 2800.692 kb on + strand, within LRK53_RS12655 at 2800.692 kb on + strand, within LRK53_RS12655 at 2800.692 kb on + strand, within LRK53_RS12655 at 2800.811 kb on + strand, within LRK53_RS12655 at 2800.811 kb on + strand, within LRK53_RS12655 at 2800.811 kb on + strand, within LRK53_RS12655 at 2800.811 kb on + strand, within LRK53_RS12655 at 2800.811 kb on + strand, within LRK53_RS12655 at 2800.811 kb on + strand, within LRK53_RS12655 at 2800.812 kb on - strand, within LRK53_RS12655 at 2800.812 kb on - strand, within LRK53_RS12655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 2,798,717 + LRK53_RS12640 0.75 -0.0 2,798,717 + LRK53_RS12640 0.75 +1.5 2,798,718 - LRK53_RS12640 0.75 -0.0 2,798,718 - LRK53_RS12640 0.75 +2.5 2,798,718 - LRK53_RS12640 0.75 -1.4 2,798,718 - LRK53_RS12640 0.75 +1.0 2,798,812 + -0.0 2,798,813 - +0.2 2,798,813 - -1.4 2,798,987 + +0.2 2,798,987 + -0.6 2,798,988 - -1.0 2,798,988 - -0.8 2,799,000 + -1.8 2,799,000 + -1.6 2,799,000 + -0.2 2,799,000 + -0.4 2,799,000 + -1.0 2,799,000 + +1.5 2,799,000 + -0.0 2,799,027 + -0.5 2,799,027 + +2.5 2,799,027 + +0.5 2,799,027 + -1.4 2,799,028 - -1.8 2,799,028 - +0.7 2,799,028 - +1.5 2,799,028 - -0.6 2,799,028 - -0.6 2,799,076 - LRK53_RS12645 0.17 -0.5 2,799,076 - LRK53_RS12645 0.17 -1.2 2,799,294 + LRK53_RS12645 0.61 -1.6 2,799,294 + LRK53_RS12645 0.61 +0.4 2,799,294 + LRK53_RS12645 0.61 +0.2 2,799,379 - LRK53_RS12645 0.78 +0.6 2,799,390 + LRK53_RS12645 0.80 -0.6 2,799,390 + LRK53_RS12645 0.80 +1.5 2,799,390 + LRK53_RS12645 0.80 -0.9 2,799,390 + LRK53_RS12645 0.80 +0.2 2,799,390 + LRK53_RS12645 0.80 +0.5 2,799,390 + LRK53_RS12645 0.80 -1.0 2,799,391 - LRK53_RS12645 0.80 +1.0 2,799,391 - LRK53_RS12645 0.80 -0.8 2,799,391 - LRK53_RS12645 0.80 +1.0 2,799,391 - LRK53_RS12645 0.80 -1.0 2,799,408 + LRK53_RS12645 0.83 -1.0 2,799,409 - LRK53_RS12645 0.84 -0.2 2,799,610 + LRK53_RS12650 0.16 +0.1 2,799,610 + LRK53_RS12650 0.16 +1.0 2,799,610 + LRK53_RS12650 0.16 -0.9 2,799,610 + LRK53_RS12650 0.16 +1.0 2,799,610 + LRK53_RS12650 0.16 -0.8 2,799,610 + LRK53_RS12650 0.16 +0.4 2,799,610 + LRK53_RS12650 0.16 -1.0 2,799,610 + LRK53_RS12650 0.16 -1.4 2,799,610 + LRK53_RS12650 0.16 -0.6 2,799,610 + LRK53_RS12650 0.16 -1.4 2,799,610 + LRK53_RS12650 0.16 -0.4 2,799,611 - LRK53_RS12650 0.16 -1.0 2,799,611 - LRK53_RS12650 0.16 -0.8 2,799,611 - LRK53_RS12650 0.16 -2.5 2,799,611 - LRK53_RS12650 0.16 -0.0 2,799,611 - LRK53_RS12650 0.16 -0.0 2,799,611 - LRK53_RS12650 0.16 -1.2 2,799,611 - LRK53_RS12650 0.16 -0.6 2,799,611 - LRK53_RS12650 0.16 +2.5 2,799,611 - LRK53_RS12650 0.16 -0.7 2,799,611 - LRK53_RS12650 0.16 +1.5 2,799,611 - LRK53_RS12650 0.16 -0.0 2,799,611 - LRK53_RS12650 0.16 -0.0 2,799,611 - LRK53_RS12650 0.16 +1.3 2,799,617 - LRK53_RS12650 0.17 -0.0 2,799,628 + LRK53_RS12650 0.19 +1.0 2,799,628 + LRK53_RS12650 0.19 -0.6 2,799,925 - LRK53_RS12650 0.70 -1.6 2,799,925 - LRK53_RS12650 0.70 -3.5 2,799,925 - LRK53_RS12650 0.70 -0.8 2,799,952 + LRK53_RS12650 0.75 -0.2 2,799,952 + LRK53_RS12650 0.75 -0.2 2,799,952 + LRK53_RS12650 0.75 -0.8 2,799,952 + LRK53_RS12650 0.75 -1.9 2,799,952 + LRK53_RS12650 0.75 -2.6 2,799,952 + LRK53_RS12650 0.75 -1.7 2,799,952 + LRK53_RS12650 0.75 -0.0 2,799,952 + LRK53_RS12650 0.75 +0.1 2,799,952 + LRK53_RS12650 0.75 +1.0 2,799,952 + LRK53_RS12650 0.75 +1.0 2,799,952 + LRK53_RS12650 0.75 -1.4 2,799,952 + LRK53_RS12650 0.75 -0.6 2,799,953 - LRK53_RS12650 0.75 +0.2 2,799,953 - LRK53_RS12650 0.75 -0.0 2,799,953 - LRK53_RS12650 0.75 -0.6 2,799,953 - LRK53_RS12650 0.75 +0.4 2,799,953 - LRK53_RS12650 0.75 -2.2 2,799,953 - LRK53_RS12650 0.75 -0.7 2,799,953 - LRK53_RS12650 0.75 +0.7 2,799,953 - LRK53_RS12650 0.75 +1.0 2,799,953 - LRK53_RS12650 0.75 -1.1 2,799,953 - LRK53_RS12650 0.75 -0.0 2,800,051 - -0.0 2,800,074 + -0.2 2,800,074 + -0.4 2,800,074 + +0.5 2,800,074 + -0.9 2,800,074 + +0.5 2,800,075 - -0.8 2,800,075 - +0.3 2,800,075 - -0.4 2,800,075 - +0.5 2,800,075 - +1.0 2,800,075 - -1.2 2,800,075 - -0.9 2,800,075 - -3.1 2,800,075 - +1.0 2,800,075 - -1.0 2,800,075 - -0.7 2,800,075 - -1.4 2,800,075 - +0.5 2,800,075 - -0.5 2,800,161 + -0.6 2,800,161 + -1.0 2,800,161 + -1.0 2,800,161 + -1.8 2,800,161 + +0.6 2,800,161 + +0.5 2,800,161 + -1.3 2,800,161 + -0.0 2,800,161 + -2.8 2,800,162 - -0.4 2,800,162 - -0.5 2,800,162 - +0.4 2,800,162 - -0.0 2,800,162 - +0.4 2,800,203 + +0.4 2,800,203 + +0.5 2,800,203 + +1.0 2,800,203 + -1.4 2,800,203 + -0.0 2,800,203 + +1.0 2,800,203 + +0.2 2,800,203 + +0.5 2,800,203 + -1.9 2,800,203 + +0.5 2,800,204 - +1.0 2,800,204 - -0.3 2,800,217 + -1.0 2,800,217 + -1.4 2,800,218 - -1.4 2,800,218 - -2.1 2,800,218 - +1.0 2,800,222 + -0.8 2,800,222 + -0.0 2,800,222 + +0.1 2,800,223 - +0.2 2,800,223 - -0.4 2,800,457 + LRK53_RS12655 0.26 +0.2 2,800,692 + LRK53_RS12655 0.55 +1.0 2,800,692 + LRK53_RS12655 0.55 +0.6 2,800,692 + LRK53_RS12655 0.55 -0.5 2,800,811 + LRK53_RS12655 0.71 -1.2 2,800,811 + LRK53_RS12655 0.71 -1.2 2,800,811 + LRK53_RS12655 0.71 +1.6 2,800,811 + LRK53_RS12655 0.71 +0.4 2,800,811 + LRK53_RS12655 0.71 +1.6 2,800,811 + LRK53_RS12655 0.71 -0.3 2,800,812 - LRK53_RS12655 0.71 -0.6 2,800,812 - LRK53_RS12655 0.71 +1.3
Or see this region's nucleotide sequence