Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09255 and LRK53_RS09260 overlap by 4 nucleotides LRK53_RS09260 and LRK53_RS09265 overlap by 4 nucleotides LRK53_RS09265 and LRK53_RS09270 are separated by 3 nucleotides LRK53_RS09270 and LRK53_RS09275 are separated by 251 nucleotides
LRK53_RS09255: LRK53_RS09255 - ABC-2 transporter permease, at 2,069,937 to 2,070,920
_RS09255
LRK53_RS09260: LRK53_RS09260 - ABC transporter ATP-binding protein, at 2,070,917 to 2,071,774
_RS09260
LRK53_RS09265: LRK53_RS09265 - GntR family transcriptional regulator, at 2,071,771 to 2,072,145
_RS09265
LRK53_RS09270: LRK53_RS09270 - hypothetical protein, at 2,072,149 to 2,072,490
_RS09270
LRK53_RS09275: LRK53_RS09275 - SDR family oxidoreductase, at 2,072,742 to 2,073,527
_RS09275
Position (kb)
2070
2071
2072 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2070.037 kb on + strand, within LRK53_RS09255 at 2070.037 kb on + strand, within LRK53_RS09255 at 2070.037 kb on + strand, within LRK53_RS09255 at 2070.037 kb on + strand, within LRK53_RS09255 at 2070.038 kb on - strand, within LRK53_RS09255 at 2070.038 kb on - strand, within LRK53_RS09255 at 2070.038 kb on - strand, within LRK53_RS09255 at 2070.038 kb on - strand, within LRK53_RS09255 at 2070.038 kb on - strand, within LRK53_RS09255 at 2070.041 kb on + strand, within LRK53_RS09255 at 2070.041 kb on + strand, within LRK53_RS09255 at 2070.041 kb on + strand, within LRK53_RS09255 at 2070.041 kb on + strand, within LRK53_RS09255 at 2070.042 kb on - strand, within LRK53_RS09255 at 2070.042 kb on - strand, within LRK53_RS09255 at 2070.042 kb on - strand, within LRK53_RS09255 at 2070.042 kb on - strand, within LRK53_RS09255 at 2070.042 kb on - strand, within LRK53_RS09255 at 2070.042 kb on - strand, within LRK53_RS09255 at 2070.042 kb on - strand, within LRK53_RS09255 at 2070.154 kb on - strand, within LRK53_RS09255 at 2070.154 kb on - strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.349 kb on + strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.350 kb on - strand, within LRK53_RS09255 at 2070.366 kb on + strand, within LRK53_RS09255 at 2070.367 kb on - strand, within LRK53_RS09255 at 2070.367 kb on - strand, within LRK53_RS09255 at 2070.610 kb on + strand, within LRK53_RS09255 at 2070.628 kb on + strand, within LRK53_RS09255 at 2070.628 kb on + strand, within LRK53_RS09255 at 2070.628 kb on + strand, within LRK53_RS09255 at 2070.629 kb on - strand, within LRK53_RS09255 at 2070.710 kb on + strand, within LRK53_RS09255 at 2070.711 kb on - strand, within LRK53_RS09255 at 2070.861 kb on + strand at 2070.862 kb on - strand at 2070.907 kb on + strand at 2070.907 kb on + strand at 2070.907 kb on + strand at 2070.907 kb on + strand at 2070.907 kb on + strand at 2070.907 kb on + strand at 2070.908 kb on - strand at 2070.908 kb on - strand at 2070.908 kb on - strand at 2070.908 kb on - strand at 2070.908 kb on - strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.924 kb on + strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2070.925 kb on - strand at 2071.040 kb on + strand, within LRK53_RS09260 at 2071.041 kb on - strand, within LRK53_RS09260 at 2071.248 kb on + strand, within LRK53_RS09260 at 2071.248 kb on + strand, within LRK53_RS09260 at 2071.248 kb on + strand, within LRK53_RS09260 at 2071.249 kb on - strand, within LRK53_RS09260 at 2071.249 kb on - strand, within LRK53_RS09260 at 2071.249 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.260 kb on - strand, within LRK53_RS09260 at 2071.269 kb on + strand, within LRK53_RS09260 at 2071.269 kb on + strand, within LRK53_RS09260 at 2071.270 kb on - strand, within LRK53_RS09260 at 2071.270 kb on - strand, within LRK53_RS09260 at 2071.270 kb on - strand, within LRK53_RS09260 at 2071.270 kb on - strand, within LRK53_RS09260 at 2071.581 kb on + strand, within LRK53_RS09260 at 2071.734 kb on + strand at 2071.734 kb on + strand at 2071.734 kb on + strand at 2071.734 kb on + strand at 2071.734 kb on + strand at 2071.735 kb on - strand at 2071.735 kb on - strand at 2071.735 kb on - strand at 2071.735 kb on - strand at 2071.982 kb on + strand, within LRK53_RS09265 at 2071.982 kb on + strand, within LRK53_RS09265 at 2071.982 kb on + strand, within LRK53_RS09265 at 2071.983 kb on - strand, within LRK53_RS09265 at 2071.983 kb on - strand, within LRK53_RS09265 at 2072.111 kb on + strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.112 kb on - strand at 2072.150 kb on + strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.151 kb on - strand at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.189 kb on + strand, within LRK53_RS09270 at 2072.190 kb on - strand, within LRK53_RS09270 at 2072.192 kb on + strand, within LRK53_RS09270 at 2072.192 kb on + strand, within LRK53_RS09270 at 2072.193 kb on - strand, within LRK53_RS09270 at 2072.193 kb on - strand, within LRK53_RS09270 at 2072.262 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.417 kb on + strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.418 kb on - strand, within LRK53_RS09270 at 2072.562 kb on - strand at 2072.597 kb on + strand at 2072.597 kb on + strand at 2072.597 kb on + strand at 2072.597 kb on + strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand at 2072.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 2,070,037 + LRK53_RS09255 0.10 +0.6 2,070,037 + LRK53_RS09255 0.10 -1.3 2,070,037 + LRK53_RS09255 0.10 +0.0 2,070,037 + LRK53_RS09255 0.10 +1.0 2,070,038 - LRK53_RS09255 0.10 -1.6 2,070,038 - LRK53_RS09255 0.10 +0.4 2,070,038 - LRK53_RS09255 0.10 +1.0 2,070,038 - LRK53_RS09255 0.10 -0.0 2,070,038 - LRK53_RS09255 0.10 -0.1 2,070,041 + LRK53_RS09255 0.11 +1.3 2,070,041 + LRK53_RS09255 0.11 -0.9 2,070,041 + LRK53_RS09255 0.11 -2.5 2,070,041 + LRK53_RS09255 0.11 +1.0 2,070,042 - LRK53_RS09255 0.11 -2.6 2,070,042 - LRK53_RS09255 0.11 +0.0 2,070,042 - LRK53_RS09255 0.11 +0.5 2,070,042 - LRK53_RS09255 0.11 +0.0 2,070,042 - LRK53_RS09255 0.11 +0.4 2,070,042 - LRK53_RS09255 0.11 +0.2 2,070,042 - LRK53_RS09255 0.11 +1.3 2,070,154 - LRK53_RS09255 0.22 +1.0 2,070,154 - LRK53_RS09255 0.22 -1.0 2,070,349 + LRK53_RS09255 0.42 -1.3 2,070,349 + LRK53_RS09255 0.42 -0.9 2,070,349 + LRK53_RS09255 0.42 -1.3 2,070,349 + LRK53_RS09255 0.42 +0.0 2,070,349 + LRK53_RS09255 0.42 +0.0 2,070,349 + LRK53_RS09255 0.42 +0.2 2,070,349 + LRK53_RS09255 0.42 +2.0 2,070,349 + LRK53_RS09255 0.42 +0.1 2,070,350 - LRK53_RS09255 0.42 -0.6 2,070,350 - LRK53_RS09255 0.42 +0.4 2,070,350 - LRK53_RS09255 0.42 +1.0 2,070,350 - LRK53_RS09255 0.42 -0.6 2,070,350 - LRK53_RS09255 0.42 -1.0 2,070,350 - LRK53_RS09255 0.42 +0.5 2,070,350 - LRK53_RS09255 0.42 -2.3 2,070,350 - LRK53_RS09255 0.42 +0.0 2,070,366 + LRK53_RS09255 0.44 -1.6 2,070,367 - LRK53_RS09255 0.44 -0.6 2,070,367 - LRK53_RS09255 0.44 -1.3 2,070,610 + LRK53_RS09255 0.68 -0.6 2,070,628 + LRK53_RS09255 0.70 +1.0 2,070,628 + LRK53_RS09255 0.70 -3.4 2,070,628 + LRK53_RS09255 0.70 +1.2 2,070,629 - LRK53_RS09255 0.70 +0.0 2,070,710 + LRK53_RS09255 0.79 -0.4 2,070,711 - LRK53_RS09255 0.79 -1.8 2,070,861 + -0.6 2,070,862 - +0.4 2,070,907 + -2.8 2,070,907 + -0.6 2,070,907 + +0.2 2,070,907 + +0.0 2,070,907 + -0.9 2,070,907 + -1.4 2,070,908 - +0.4 2,070,908 - +0.0 2,070,908 - -1.2 2,070,908 - -0.1 2,070,908 - +1.0 2,070,924 + +1.0 2,070,924 + -1.3 2,070,924 + -0.6 2,070,924 + -0.8 2,070,924 + -1.2 2,070,924 + -0.3 2,070,924 + -1.3 2,070,924 + -0.2 2,070,924 + +0.3 2,070,925 - -0.7 2,070,925 - +1.0 2,070,925 - -1.5 2,070,925 - -1.0 2,070,925 - -0.8 2,070,925 - -0.6 2,070,925 - -1.6 2,070,925 - +1.3 2,070,925 - -2.4 2,071,040 + LRK53_RS09260 0.14 +1.6 2,071,041 - LRK53_RS09260 0.14 +0.0 2,071,248 + LRK53_RS09260 0.39 +1.6 2,071,248 + LRK53_RS09260 0.39 +2.0 2,071,248 + LRK53_RS09260 0.39 +1.0 2,071,249 - LRK53_RS09260 0.39 +0.6 2,071,249 - LRK53_RS09260 0.39 -0.2 2,071,249 - LRK53_RS09260 0.39 +3.0 2,071,260 - LRK53_RS09260 0.40 +2.6 2,071,260 - LRK53_RS09260 0.40 +2.6 2,071,260 - LRK53_RS09260 0.40 -0.4 2,071,260 - LRK53_RS09260 0.40 -0.1 2,071,260 - LRK53_RS09260 0.40 -0.8 2,071,260 - LRK53_RS09260 0.40 +1.0 2,071,260 - LRK53_RS09260 0.40 +0.0 2,071,260 - LRK53_RS09260 0.40 +1.0 2,071,260 - LRK53_RS09260 0.40 -0.4 2,071,269 + LRK53_RS09260 0.41 -0.5 2,071,269 + LRK53_RS09260 0.41 +0.0 2,071,270 - LRK53_RS09260 0.41 +0.4 2,071,270 - LRK53_RS09260 0.41 -0.6 2,071,270 - LRK53_RS09260 0.41 -0.8 2,071,270 - LRK53_RS09260 0.41 -0.6 2,071,581 + LRK53_RS09260 0.77 -0.6 2,071,734 + +1.0 2,071,734 + -0.2 2,071,734 + -1.8 2,071,734 + +0.0 2,071,734 + +0.7 2,071,735 - -0.3 2,071,735 - -1.6 2,071,735 - +1.0 2,071,735 - -2.0 2,071,982 + LRK53_RS09265 0.56 -2.0 2,071,982 + LRK53_RS09265 0.56 -0.6 2,071,982 + LRK53_RS09265 0.56 +0.0 2,071,983 - LRK53_RS09265 0.57 +1.0 2,071,983 - LRK53_RS09265 0.57 -0.6 2,072,111 + -1.8 2,072,112 - -0.6 2,072,112 - -1.8 2,072,112 - -0.6 2,072,112 - +0.0 2,072,112 - +0.0 2,072,150 + +0.0 2,072,151 - +0.0 2,072,151 - -1.4 2,072,151 - +0.0 2,072,151 - +1.0 2,072,151 - -2.6 2,072,151 - -2.0 2,072,151 - -0.9 2,072,189 + LRK53_RS09270 0.12 -0.4 2,072,189 + LRK53_RS09270 0.12 +0.0 2,072,189 + LRK53_RS09270 0.12 -0.6 2,072,189 + LRK53_RS09270 0.12 +0.4 2,072,189 + LRK53_RS09270 0.12 -0.6 2,072,190 - LRK53_RS09270 0.12 -0.6 2,072,192 + LRK53_RS09270 0.13 -0.6 2,072,192 + LRK53_RS09270 0.13 -0.6 2,072,193 - LRK53_RS09270 0.13 +0.4 2,072,193 - LRK53_RS09270 0.13 +0.0 2,072,262 + LRK53_RS09270 0.33 -0.6 2,072,417 + LRK53_RS09270 0.78 -1.6 2,072,417 + LRK53_RS09270 0.78 +1.6 2,072,417 + LRK53_RS09270 0.78 -0.7 2,072,417 + LRK53_RS09270 0.78 +0.3 2,072,417 + LRK53_RS09270 0.78 +1.0 2,072,417 + LRK53_RS09270 0.78 +0.0 2,072,417 + LRK53_RS09270 0.78 +0.0 2,072,418 - LRK53_RS09270 0.79 +0.2 2,072,418 - LRK53_RS09270 0.79 -0.6 2,072,418 - LRK53_RS09270 0.79 -0.4 2,072,418 - LRK53_RS09270 0.79 -0.6 2,072,562 - -0.6 2,072,597 + -0.3 2,072,597 + -0.6 2,072,597 + -0.7 2,072,597 + +0.3 2,072,598 - +1.6 2,072,598 - -1.0 2,072,598 - +1.6 2,072,598 - -0.3 2,072,598 - -0.3 2,072,598 - +0.4 2,072,598 - -0.2 2,072,598 - -0.6 2,072,598 - -1.6 2,072,598 - -0.8 2,072,598 - +0.0
Or see this region's nucleotide sequence