Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08350 and LRK53_RS08355 overlap by 4 nucleotides LRK53_RS08355 and LRK53_RS08360 are separated by 46 nucleotides
LRK53_RS08350: LRK53_RS08350 - tryptophan synthase subunit beta, at 1,876,528 to 1,877,739
_RS08350
LRK53_RS08355: LRK53_RS08355 - tryptophan synthase subunit alpha, at 1,877,736 to 1,878,536
_RS08355
LRK53_RS08360: LRK53_RS08360 - acetyl-CoA carboxylase, carboxyltransferase subunit beta, at 1,878,583 to 1,879,452
_RS08360
Position (kb)
1877
1878
1879 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1876.801 kb on - strand, within LRK53_RS08350 at 1876.951 kb on + strand, within LRK53_RS08350 at 1876.952 kb on - strand, within LRK53_RS08350 at 1876.952 kb on - strand, within LRK53_RS08350 at 1876.952 kb on - strand, within LRK53_RS08350 at 1877.110 kb on + strand, within LRK53_RS08350 at 1877.110 kb on + strand, within LRK53_RS08350 at 1877.111 kb on - strand, within LRK53_RS08350 at 1877.111 kb on - strand, within LRK53_RS08350 at 1877.111 kb on - strand, within LRK53_RS08350 at 1877.212 kb on + strand, within LRK53_RS08350 at 1877.407 kb on + strand, within LRK53_RS08350 at 1877.408 kb on - strand, within LRK53_RS08350 at 1877.524 kb on + strand, within LRK53_RS08350 at 1877.525 kb on - strand, within LRK53_RS08350 at 1877.647 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.735 kb on + strand at 1877.736 kb on - strand at 1877.736 kb on - strand at 1877.736 kb on - strand at 1877.736 kb on - strand at 1877.736 kb on - strand at 1878.255 kb on + strand, within LRK53_RS08355 at 1878.258 kb on + strand, within LRK53_RS08355 at 1878.258 kb on + strand, within LRK53_RS08355 at 1878.258 kb on + strand, within LRK53_RS08355 at 1878.258 kb on + strand, within LRK53_RS08355 at 1878.258 kb on + strand, within LRK53_RS08355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 1,876,801 - LRK53_RS08350 0.23 -1.3 1,876,951 + LRK53_RS08350 0.35 +2.0 1,876,952 - LRK53_RS08350 0.35 +0.0 1,876,952 - LRK53_RS08350 0.35 +1.0 1,876,952 - LRK53_RS08350 0.35 -2.2 1,877,110 + LRK53_RS08350 0.48 +0.0 1,877,110 + LRK53_RS08350 0.48 +1.0 1,877,111 - LRK53_RS08350 0.48 -1.8 1,877,111 - LRK53_RS08350 0.48 +1.0 1,877,111 - LRK53_RS08350 0.48 +1.0 1,877,212 + LRK53_RS08350 0.56 +1.4 1,877,407 + LRK53_RS08350 0.73 -1.0 1,877,408 - LRK53_RS08350 0.73 +1.0 1,877,524 + LRK53_RS08350 0.82 -1.3 1,877,525 - LRK53_RS08350 0.82 +1.0 1,877,647 + +1.0 1,877,735 + -0.8 1,877,735 + -0.6 1,877,735 + +1.0 1,877,735 + -0.6 1,877,735 + +0.4 1,877,735 + -0.2 1,877,735 + -1.6 1,877,735 + -1.6 1,877,735 + +1.0 1,877,736 - -1.0 1,877,736 - -1.3 1,877,736 - -0.6 1,877,736 - -1.3 1,877,736 - +0.0 1,878,255 + LRK53_RS08355 0.65 +2.0 1,878,258 + LRK53_RS08355 0.65 -0.6 1,878,258 + LRK53_RS08355 0.65 -2.5 1,878,258 + LRK53_RS08355 0.65 +1.0 1,878,258 + LRK53_RS08355 0.65 -0.6 1,878,258 + LRK53_RS08355 0.65 +1.0
Or see this region's nucleotide sequence