Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00920 and LRK53_RS00925 overlap by 4 nucleotides LRK53_RS00925 and LRK53_RS00930 overlap by 4 nucleotides LRK53_RS00930 and LRK53_RS00935 overlap by 4 nucleotides LRK53_RS00935 and LRK53_RS00940 are separated by 151 nucleotides LRK53_RS00940 and LRK53_RS00945 are separated by 383 nucleotides
LRK53_RS00920: LRK53_RS00920 - monovalent cation/H+ antiporter subunit D, at 233,504 to 235,099
_RS00920
LRK53_RS00925: LRK53_RS00925 - Na+/H+ antiporter subunit E, at 235,096 to 235,584
_RS00925
LRK53_RS00930: LRK53_RS00930 - K+/H+ antiporter subunit F, at 235,581 to 235,862
_RS00930
LRK53_RS00935: LRK53_RS00935 - monovalent cation/H(+) antiporter subunit G, at 235,859 to 236,212
_RS00935
LRK53_RS00940: LRK53_RS00940 - hypothetical protein, at 236,364 to 236,561
_RS00940
LRK53_RS00945: LRK53_RS00945 - helix-turn-helix domain-containing protein, at 236,945 to 238,063
_RS00945
Position (kb) 235
236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 234.933 kb on + strand, within LRK53_RS00920 at 234.933 kb on + strand, within LRK53_RS00920 at 234.933 kb on + strand, within LRK53_RS00920 at 234.933 kb on + strand, within LRK53_RS00920 at 234.933 kb on + strand, within LRK53_RS00920 at 234.933 kb on + strand, within LRK53_RS00920 at 234.933 kb on + strand, within LRK53_RS00920 at 234.934 kb on - strand, within LRK53_RS00920 at 234.934 kb on - strand, within LRK53_RS00920 at 234.934 kb on - strand, within LRK53_RS00920 at 234.934 kb on - strand, within LRK53_RS00920 at 234.934 kb on - strand, within LRK53_RS00920 at 235.058 kb on + strand at 235.058 kb on + strand at 235.058 kb on + strand at 235.058 kb on + strand at 235.059 kb on - strand at 235.059 kb on - strand at 235.267 kb on + strand, within LRK53_RS00925 at 235.268 kb on - strand, within LRK53_RS00925 at 235.408 kb on + strand, within LRK53_RS00925 at 235.468 kb on + strand, within LRK53_RS00925 at 235.468 kb on + strand, within LRK53_RS00925 at 235.468 kb on + strand, within LRK53_RS00925 at 235.469 kb on - strand, within LRK53_RS00925 at 235.469 kb on - strand, within LRK53_RS00925 at 235.469 kb on - strand, within LRK53_RS00925 at 235.502 kb on + strand, within LRK53_RS00925 at 235.502 kb on + strand, within LRK53_RS00925 at 235.502 kb on + strand, within LRK53_RS00925 at 235.502 kb on + strand, within LRK53_RS00925 at 235.502 kb on + strand, within LRK53_RS00925 at 235.502 kb on + strand, within LRK53_RS00925 at 235.502 kb on + strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.503 kb on - strand, within LRK53_RS00925 at 235.553 kb on - strand at 235.713 kb on + strand, within LRK53_RS00930 at 235.713 kb on + strand, within LRK53_RS00930 at 235.713 kb on + strand, within LRK53_RS00930 at 235.713 kb on + strand, within LRK53_RS00930 at 235.713 kb on + strand, within LRK53_RS00930 at 235.714 kb on - strand, within LRK53_RS00930 at 235.714 kb on - strand, within LRK53_RS00930 at 235.714 kb on - strand, within LRK53_RS00930 at 235.770 kb on + strand, within LRK53_RS00930 at 235.770 kb on + strand, within LRK53_RS00930 at 235.770 kb on + strand, within LRK53_RS00930 at 235.771 kb on - strand, within LRK53_RS00930 at 235.771 kb on - strand, within LRK53_RS00930 at 235.771 kb on - strand, within LRK53_RS00930 at 235.771 kb on - strand, within LRK53_RS00930 at 235.909 kb on - strand, within LRK53_RS00935 at 235.917 kb on + strand, within LRK53_RS00935 at 235.917 kb on + strand, within LRK53_RS00935 at 235.917 kb on + strand, within LRK53_RS00935 at 235.918 kb on - strand, within LRK53_RS00935 at 235.918 kb on - strand, within LRK53_RS00935 at 235.976 kb on + strand, within LRK53_RS00935 at 235.976 kb on + strand, within LRK53_RS00935 at 235.977 kb on - strand, within LRK53_RS00935 at 236.082 kb on - strand, within LRK53_RS00935 at 236.082 kb on - strand, within LRK53_RS00935 at 236.142 kb on + strand, within LRK53_RS00935 at 236.156 kb on + strand, within LRK53_RS00935 at 236.156 kb on + strand, within LRK53_RS00935 at 236.157 kb on - strand, within LRK53_RS00935 at 236.210 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.280 kb on + strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.281 kb on - strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.353 kb on + strand at 236.354 kb on - strand at 236.354 kb on - strand at 236.354 kb on - strand at 236.354 kb on - strand at 236.468 kb on - strand, within LRK53_RS00940 at 236.506 kb on + strand, within LRK53_RS00940 at 236.698 kb on - strand at 236.698 kb on - strand at 236.698 kb on - strand at 236.698 kb on - strand at 236.698 kb on - strand at 236.698 kb on - strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.750 kb on + strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.751 kb on - strand at 236.899 kb on - strand at 236.899 kb on - strand at 236.899 kb on - strand at 236.916 kb on + strand at 236.916 kb on + strand at 236.916 kb on + strand at 236.916 kb on + strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.917 kb on - strand at 236.943 kb on + strand at 236.944 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 236.979 kb on - strand at 237.002 kb on + strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.003 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.054 kb on - strand at 237.112 kb on + strand, within LRK53_RS00945 at 237.113 kb on - strand, within LRK53_RS00945 at 237.113 kb on - strand, within LRK53_RS00945 at 237.126 kb on - strand, within LRK53_RS00945 at 237.126 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945 at 237.207 kb on - strand, within LRK53_RS00945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 234,933 + LRK53_RS00920 0.90 -0.0 234,933 + LRK53_RS00920 0.90 -1.0 234,933 + LRK53_RS00920 0.90 -0.0 234,933 + LRK53_RS00920 0.90 +1.0 234,933 + LRK53_RS00920 0.90 +1.0 234,933 + LRK53_RS00920 0.90 -1.6 234,933 + LRK53_RS00920 0.90 -0.0 234,934 - LRK53_RS00920 0.90 +0.4 234,934 - LRK53_RS00920 0.90 -0.3 234,934 - LRK53_RS00920 0.90 +1.0 234,934 - LRK53_RS00920 0.90 +3.0 234,934 - LRK53_RS00920 0.90 +1.0 235,058 + +2.0 235,058 + -1.8 235,058 + -0.6 235,058 + +0.4 235,059 - -1.3 235,059 - -0.8 235,267 + LRK53_RS00925 0.35 -1.0 235,268 - LRK53_RS00925 0.35 -1.3 235,408 + LRK53_RS00925 0.64 +1.0 235,468 + LRK53_RS00925 0.76 +1.0 235,468 + LRK53_RS00925 0.76 -1.8 235,468 + LRK53_RS00925 0.76 -0.6 235,469 - LRK53_RS00925 0.76 -0.0 235,469 - LRK53_RS00925 0.76 -1.0 235,469 - LRK53_RS00925 0.76 +1.0 235,502 + LRK53_RS00925 0.83 +0.4 235,502 + LRK53_RS00925 0.83 -0.0 235,502 + LRK53_RS00925 0.83 -1.6 235,502 + LRK53_RS00925 0.83 +1.6 235,502 + LRK53_RS00925 0.83 -1.3 235,502 + LRK53_RS00925 0.83 +1.6 235,502 + LRK53_RS00925 0.83 -0.0 235,503 - LRK53_RS00925 0.83 +2.6 235,503 - LRK53_RS00925 0.83 +0.4 235,503 - LRK53_RS00925 0.83 -1.3 235,503 - LRK53_RS00925 0.83 -1.0 235,503 - LRK53_RS00925 0.83 -0.6 235,503 - LRK53_RS00925 0.83 -1.3 235,503 - LRK53_RS00925 0.83 -0.6 235,503 - LRK53_RS00925 0.83 -0.0 235,503 - LRK53_RS00925 0.83 -0.6 235,503 - LRK53_RS00925 0.83 -1.0 235,503 - LRK53_RS00925 0.83 -0.0 235,503 - LRK53_RS00925 0.83 +0.7 235,503 - LRK53_RS00925 0.83 -1.3 235,553 - -1.6 235,713 + LRK53_RS00930 0.47 -0.0 235,713 + LRK53_RS00930 0.47 -0.0 235,713 + LRK53_RS00930 0.47 -1.2 235,713 + LRK53_RS00930 0.47 +0.4 235,713 + LRK53_RS00930 0.47 -0.2 235,714 - LRK53_RS00930 0.47 -3.2 235,714 - LRK53_RS00930 0.47 -0.0 235,714 - LRK53_RS00930 0.47 +0.4 235,770 + LRK53_RS00930 0.67 +0.2 235,770 + LRK53_RS00930 0.67 -0.0 235,770 + LRK53_RS00930 0.67 +1.0 235,771 - LRK53_RS00930 0.67 +0.3 235,771 - LRK53_RS00930 0.67 +1.3 235,771 - LRK53_RS00930 0.67 -0.0 235,771 - LRK53_RS00930 0.67 -0.6 235,909 - LRK53_RS00935 0.14 -0.6 235,917 + LRK53_RS00935 0.16 +0.4 235,917 + LRK53_RS00935 0.16 +1.6 235,917 + LRK53_RS00935 0.16 -1.0 235,918 - LRK53_RS00935 0.17 -0.0 235,918 - LRK53_RS00935 0.17 -1.2 235,976 + LRK53_RS00935 0.33 +0.4 235,976 + LRK53_RS00935 0.33 -0.7 235,977 - LRK53_RS00935 0.33 -1.1 236,082 - LRK53_RS00935 0.63 +1.0 236,082 - LRK53_RS00935 0.63 -0.6 236,142 + LRK53_RS00935 0.80 -1.6 236,156 + LRK53_RS00935 0.84 -1.4 236,156 + LRK53_RS00935 0.84 +0.7 236,157 - LRK53_RS00935 0.84 -0.0 236,210 + -2.2 236,280 + -0.2 236,280 + -2.7 236,280 + -0.3 236,280 + -1.6 236,280 + +1.0 236,281 - -1.6 236,281 - +1.6 236,281 - -0.0 236,281 - -0.4 236,281 - -0.8 236,281 - +1.0 236,353 + -0.9 236,353 + +1.6 236,353 + -0.3 236,353 + -1.2 236,353 + +2.0 236,354 - +1.0 236,354 - -0.2 236,354 - -0.6 236,354 - -0.0 236,468 - LRK53_RS00940 0.53 -1.1 236,506 + LRK53_RS00940 0.72 -0.6 236,698 - -0.5 236,698 - -0.0 236,698 - -0.6 236,698 - -0.0 236,698 - +1.6 236,698 - +0.6 236,750 + -0.6 236,750 + -0.6 236,750 + -0.6 236,750 + +1.0 236,750 + +0.6 236,750 + -0.0 236,750 + -0.6 236,750 + -1.8 236,750 + -0.9 236,750 + -0.0 236,750 + +0.4 236,751 - -1.3 236,751 - -1.6 236,751 - -0.6 236,751 - -0.5 236,751 - +0.2 236,751 - +0.4 236,751 - -0.0 236,751 - +1.0 236,751 - +0.4 236,751 - -0.6 236,751 - +0.7 236,751 - -0.0 236,751 - +0.1 236,751 - +1.0 236,751 - +1.0 236,899 - +0.6 236,899 - -0.6 236,899 - +1.0 236,916 + -2.2 236,916 + -0.3 236,916 + -0.3 236,916 + -1.6 236,917 - -0.2 236,917 - +1.0 236,917 - -1.1 236,917 - -0.6 236,917 - +0.4 236,943 + -0.0 236,944 - +0.6 236,979 - -1.0 236,979 - +1.0 236,979 - -0.0 236,979 - +0.1 236,979 - -2.2 236,979 - -1.0 236,979 - -1.7 236,979 - -0.0 236,979 - -1.3 236,979 - -0.6 236,979 - -1.8 237,002 + -1.5 237,003 - +0.1 237,003 - +0.2 237,003 - -1.3 237,003 - -1.8 237,003 - -1.8 237,003 - +0.1 237,003 - -0.6 237,003 - -1.3 237,003 - -0.5 237,003 - +1.0 237,054 - -1.1 237,054 - +0.2 237,054 - -1.0 237,054 - +1.0 237,054 - -0.6 237,054 - +0.4 237,054 - -0.4 237,054 - -1.6 237,054 - -0.0 237,054 - -0.6 237,054 - -1.0 237,054 - -0.2 237,054 - -1.3 237,054 - -1.3 237,054 - +0.3 237,054 - -0.3 237,054 - -0.8 237,054 - -2.3 237,054 - +1.0 237,112 + LRK53_RS00945 0.15 -1.0 237,113 - LRK53_RS00945 0.15 -1.0 237,113 - LRK53_RS00945 0.15 +0.4 237,126 - LRK53_RS00945 0.16 -1.6 237,126 - LRK53_RS00945 0.16 -0.6 237,207 - LRK53_RS00945 0.23 -0.0 237,207 - LRK53_RS00945 0.23 +0.6 237,207 - LRK53_RS00945 0.23 +1.0 237,207 - LRK53_RS00945 0.23 -1.0 237,207 - LRK53_RS00945 0.23 +0.4
Or see this region's nucleotide sequence