Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00010 and LRK53_RS00015 are separated by 96 nucleotides LRK53_RS00015 and LRK53_RS00020 are separated by 95 nucleotides
LRK53_RS00010: LRK53_RS00010 - DNA polymerase III subunit beta, at 1,576 to 2,676
_RS00010
LRK53_RS00015: LRK53_RS00015 - DNA replication/repair protein RecF, at 2,773 to 3,852
_RS00015
LRK53_RS00020: LRK53_RS00020 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 3,948 to 6,380
_RS00020
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.814 kb on + strand at 2.815 kb on - strand at 2.815 kb on - strand at 3.173 kb on - strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.354 kb on + strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.355 kb on - strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.358 kb on + strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.359 kb on - strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.516 kb on + strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.517 kb on - strand, within LRK53_RS00015 at 3.606 kb on + strand, within LRK53_RS00015 at 3.607 kb on - strand, within LRK53_RS00015 at 3.644 kb on - strand, within LRK53_RS00015 at 3.644 kb on - strand, within LRK53_RS00015 at 3.644 kb on - strand, within LRK53_RS00015 at 3.848 kb on + strand at 3.848 kb on + strand at 3.848 kb on + strand at 3.848 kb on + strand at 3.849 kb on - strand at 3.850 kb on + strand at 3.850 kb on + strand at 3.850 kb on + strand at 3.850 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.854 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 2,814 + -1.0 2,814 + -1.0 2,814 + +1.0 2,814 + +0.2 2,814 + -1.3 2,814 + -2.5 2,815 - -0.3 2,815 - -0.0 3,173 - LRK53_RS00015 0.37 -0.6 3,354 + LRK53_RS00015 0.54 -1.0 3,354 + LRK53_RS00015 0.54 -1.1 3,354 + LRK53_RS00015 0.54 -0.6 3,354 + LRK53_RS00015 0.54 -2.0 3,354 + LRK53_RS00015 0.54 -1.0 3,354 + LRK53_RS00015 0.54 -0.0 3,354 + LRK53_RS00015 0.54 -0.6 3,354 + LRK53_RS00015 0.54 -0.6 3,354 + LRK53_RS00015 0.54 -1.7 3,354 + LRK53_RS00015 0.54 +0.4 3,355 - LRK53_RS00015 0.54 -0.0 3,355 - LRK53_RS00015 0.54 -0.6 3,355 - LRK53_RS00015 0.54 -1.0 3,355 - LRK53_RS00015 0.54 -0.8 3,355 - LRK53_RS00015 0.54 -0.3 3,355 - LRK53_RS00015 0.54 -0.0 3,358 + LRK53_RS00015 0.54 -0.0 3,358 + LRK53_RS00015 0.54 -1.8 3,358 + LRK53_RS00015 0.54 +2.0 3,358 + LRK53_RS00015 0.54 +2.6 3,358 + LRK53_RS00015 0.54 -0.6 3,358 + LRK53_RS00015 0.54 -0.6 3,359 - LRK53_RS00015 0.54 -0.6 3,359 - LRK53_RS00015 0.54 -1.3 3,359 - LRK53_RS00015 0.54 -0.0 3,359 - LRK53_RS00015 0.54 -1.0 3,359 - LRK53_RS00015 0.54 +0.4 3,516 + LRK53_RS00015 0.69 -0.5 3,516 + LRK53_RS00015 0.69 -0.0 3,516 + LRK53_RS00015 0.69 -0.7 3,516 + LRK53_RS00015 0.69 -0.0 3,516 + LRK53_RS00015 0.69 -1.3 3,516 + LRK53_RS00015 0.69 -0.6 3,517 - LRK53_RS00015 0.69 -0.9 3,517 - LRK53_RS00015 0.69 -0.6 3,517 - LRK53_RS00015 0.69 -0.0 3,517 - LRK53_RS00015 0.69 -0.0 3,517 - LRK53_RS00015 0.69 -1.0 3,517 - LRK53_RS00015 0.69 -0.8 3,517 - LRK53_RS00015 0.69 +0.3 3,517 - LRK53_RS00015 0.69 -1.6 3,517 - LRK53_RS00015 0.69 -1.3 3,517 - LRK53_RS00015 0.69 -1.6 3,606 + LRK53_RS00015 0.77 +1.0 3,607 - LRK53_RS00015 0.77 -1.3 3,644 - LRK53_RS00015 0.81 +1.0 3,644 - LRK53_RS00015 0.81 -0.8 3,644 - LRK53_RS00015 0.81 -0.6 3,848 + -1.0 3,848 + -0.0 3,848 + -2.6 3,848 + -0.0 3,849 - -0.0 3,850 + -0.3 3,850 + -1.8 3,850 + -0.0 3,850 + +2.6 3,854 + +0.2 3,854 + -0.8 3,854 + -0.0 3,854 + +0.6 3,854 + +1.0 3,854 + -1.6 3,854 + +0.8 3,854 + +1.4 3,854 + +0.6 3,854 + -1.6 3,854 + -0.6 3,854 + +1.6
Or see this region's nucleotide sequence