Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS13915 and LRK53_RS13920 are separated by 137 nucleotides LRK53_RS13920 and LRK53_RS13925 are separated by 190 nucleotides LRK53_RS13925 and LRK53_RS13930 are separated by 4 nucleotides LRK53_RS13930 and LRK53_RS13935 are separated by 205 nucleotides
LRK53_RS13915: LRK53_RS13915 - NAD(P)/FAD-dependent oxidoreductase, at 3,065,002 to 3,065,970
_RS13915
LRK53_RS13920: LRK53_RS13920 - YajQ family cyclic di-GMP-binding protein, at 3,066,108 to 3,066,590
_RS13920
LRK53_RS13925: LRK53_RS13925 - NrsF family protein, at 3,066,781 to 3,067,434
_RS13925
LRK53_RS13930: LRK53_RS13930 - RNA polymerase sigma factor, at 3,067,439 to 3,068,026
_RS13930
LRK53_RS13935: LRK53_RS13935 - cytochrome c biogenesis protein DipZ, at 3,068,232 to 3,069,953
_RS13935
Position (kb)
3066
3067
3068 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3065.950 kb on + strand at 3065.950 kb on + strand at 3065.950 kb on + strand at 3065.950 kb on + strand at 3065.951 kb on - strand at 3065.951 kb on - strand at 3065.951 kb on - strand at 3065.951 kb on - strand at 3065.951 kb on - strand at 3065.951 kb on - strand at 3066.052 kb on + strand at 3066.052 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.072 kb on + strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.073 kb on - strand at 3066.090 kb on + strand at 3066.090 kb on + strand at 3066.091 kb on - strand at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.222 kb on + strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.223 kb on - strand, within LRK53_RS13920 at 3066.386 kb on + strand, within LRK53_RS13920 at 3066.386 kb on + strand, within LRK53_RS13920 at 3066.386 kb on + strand, within LRK53_RS13920 at 3066.386 kb on + strand, within LRK53_RS13920 at 3066.386 kb on + strand, within LRK53_RS13920 at 3066.387 kb on - strand, within LRK53_RS13920 at 3066.606 kb on - strand at 3066.730 kb on + strand at 3066.730 kb on + strand at 3066.730 kb on + strand at 3066.730 kb on + strand at 3066.730 kb on + strand at 3066.730 kb on + strand at 3066.731 kb on - strand at 3066.731 kb on - strand at 3066.731 kb on - strand at 3066.763 kb on + strand at 3066.763 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.782 kb on + strand at 3066.881 kb on + strand, within LRK53_RS13925 at 3066.881 kb on + strand, within LRK53_RS13925 at 3066.881 kb on + strand, within LRK53_RS13925 at 3066.882 kb on - strand, within LRK53_RS13925 at 3066.994 kb on + strand, within LRK53_RS13925 at 3066.995 kb on - strand, within LRK53_RS13925 at 3067.289 kb on + strand, within LRK53_RS13925 at 3067.289 kb on + strand, within LRK53_RS13925 at 3067.513 kb on + strand, within LRK53_RS13930 at 3067.513 kb on + strand, within LRK53_RS13930 at 3067.513 kb on + strand, within LRK53_RS13930 at 3067.514 kb on - strand, within LRK53_RS13930 at 3067.769 kb on + strand, within LRK53_RS13930 at 3067.770 kb on - strand, within LRK53_RS13930 at 3067.782 kb on + strand, within LRK53_RS13930 at 3067.782 kb on + strand, within LRK53_RS13930 at 3067.782 kb on + strand, within LRK53_RS13930 at 3067.782 kb on + strand, within LRK53_RS13930 at 3067.783 kb on - strand, within LRK53_RS13930 at 3067.783 kb on - strand, within LRK53_RS13930 at 3067.783 kb on - strand, within LRK53_RS13930 at 3067.783 kb on - strand, within LRK53_RS13930 at 3067.783 kb on - strand, within LRK53_RS13930 at 3067.783 kb on - strand, within LRK53_RS13930 at 3067.783 kb on - strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.800 kb on + strand, within LRK53_RS13930 at 3067.801 kb on - strand, within LRK53_RS13930 at 3067.801 kb on - strand, within LRK53_RS13930 at 3067.801 kb on - strand, within LRK53_RS13930 at 3067.801 kb on - strand, within LRK53_RS13930 at 3067.801 kb on - strand, within LRK53_RS13930 at 3067.801 kb on - strand, within LRK53_RS13930 at 3067.801 kb on - strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.804 kb on + strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.805 kb on - strand, within LRK53_RS13930 at 3067.929 kb on + strand, within LRK53_RS13930 at 3067.930 kb on - strand, within LRK53_RS13930 at 3067.930 kb on - strand, within LRK53_RS13930 at 3068.062 kb on + strand at 3068.062 kb on + strand at 3068.062 kb on + strand at 3068.062 kb on + strand at 3068.063 kb on - strand at 3068.063 kb on - strand at 3068.063 kb on - strand at 3068.063 kb on - strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.066 kb on + strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.067 kb on - strand at 3068.167 kb on + strand at 3068.167 kb on + strand at 3068.167 kb on + strand at 3068.168 kb on - strand at 3068.178 kb on - strand at 3068.224 kb on + strand at 3068.225 kb on - strand at 3068.225 kb on - strand at 3068.225 kb on - strand at 3068.253 kb on + strand at 3068.253 kb on + strand at 3068.253 kb on + strand at 3068.253 kb on + strand at 3068.254 kb on - strand at 3068.254 kb on - strand at 3068.267 kb on - strand at 3068.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 3,065,950 + +0.4 3,065,950 + +0.3 3,065,950 + -1.1 3,065,950 + -0.1 3,065,951 - +0.9 3,065,951 - -0.5 3,065,951 - -0.7 3,065,951 - +0.1 3,065,951 - -0.4 3,065,951 - +0.5 3,066,052 + -0.8 3,066,052 + -1.4 3,066,072 + -1.5 3,066,072 + -0.1 3,066,072 + +0.9 3,066,072 + -1.1 3,066,072 + -1.1 3,066,072 + -0.1 3,066,072 + +1.4 3,066,072 + -0.1 3,066,072 + -0.5 3,066,072 + -2.4 3,066,072 + -0.9 3,066,072 + -0.1 3,066,072 + -0.6 3,066,072 + -1.1 3,066,073 - +0.4 3,066,073 - -1.4 3,066,073 - +0.3 3,066,073 - -1.8 3,066,073 - -0.7 3,066,073 - +0.1 3,066,073 - -0.4 3,066,073 - -0.1 3,066,073 - +2.5 3,066,073 - -1.5 3,066,073 - -0.4 3,066,073 - -0.2 3,066,090 + -0.1 3,066,090 + -1.6 3,066,091 - -1.5 3,066,222 + LRK53_RS13920 0.24 -1.1 3,066,222 + LRK53_RS13920 0.24 +0.9 3,066,222 + LRK53_RS13920 0.24 -0.1 3,066,222 + LRK53_RS13920 0.24 -1.1 3,066,222 + LRK53_RS13920 0.24 -0.4 3,066,222 + LRK53_RS13920 0.24 -0.3 3,066,222 + LRK53_RS13920 0.24 -0.1 3,066,222 + LRK53_RS13920 0.24 +1.7 3,066,223 - LRK53_RS13920 0.24 -0.5 3,066,223 - LRK53_RS13920 0.24 -2.2 3,066,223 - LRK53_RS13920 0.24 -0.5 3,066,223 - LRK53_RS13920 0.24 -0.8 3,066,223 - LRK53_RS13920 0.24 -1.1 3,066,223 - LRK53_RS13920 0.24 -0.6 3,066,223 - LRK53_RS13920 0.24 +0.4 3,066,223 - LRK53_RS13920 0.24 -1.4 3,066,223 - LRK53_RS13920 0.24 -0.6 3,066,223 - LRK53_RS13920 0.24 -1.4 3,066,223 - LRK53_RS13920 0.24 -0.4 3,066,223 - LRK53_RS13920 0.24 -1.1 3,066,223 - LRK53_RS13920 0.24 +0.9 3,066,223 - LRK53_RS13920 0.24 -0.6 3,066,223 - LRK53_RS13920 0.24 +2.5 3,066,386 + LRK53_RS13920 0.58 -0.3 3,066,386 + LRK53_RS13920 0.58 -0.1 3,066,386 + LRK53_RS13920 0.58 +1.9 3,066,386 + LRK53_RS13920 0.58 +0.4 3,066,386 + LRK53_RS13920 0.58 +1.9 3,066,387 - LRK53_RS13920 0.58 -1.4 3,066,606 - -0.1 3,066,730 + -0.4 3,066,730 + -0.1 3,066,730 + -1.7 3,066,730 + +0.2 3,066,730 + +2.5 3,066,730 + -1.1 3,066,731 - -0.6 3,066,731 - -1.6 3,066,731 - -1.8 3,066,763 + -1.4 3,066,763 + -0.1 3,066,782 + +1.5 3,066,782 + -0.9 3,066,782 + -0.8 3,066,782 + +0.9 3,066,782 + -0.3 3,066,782 + +0.4 3,066,782 + +0.4 3,066,782 + -0.1 3,066,782 + -1.9 3,066,881 + LRK53_RS13925 0.15 -0.6 3,066,881 + LRK53_RS13925 0.15 +0.2 3,066,881 + LRK53_RS13925 0.15 -1.1 3,066,882 - LRK53_RS13925 0.15 -2.0 3,066,994 + LRK53_RS13925 0.33 -0.4 3,066,995 - LRK53_RS13925 0.33 -1.9 3,067,289 + LRK53_RS13925 0.78 -1.2 3,067,289 + LRK53_RS13925 0.78 -0.6 3,067,513 + LRK53_RS13930 0.13 -1.1 3,067,513 + LRK53_RS13930 0.13 -0.3 3,067,513 + LRK53_RS13930 0.13 -1.2 3,067,514 - LRK53_RS13930 0.13 -0.3 3,067,769 + LRK53_RS13930 0.56 -1.1 3,067,770 - LRK53_RS13930 0.56 +1.4 3,067,782 + LRK53_RS13930 0.58 -0.5 3,067,782 + LRK53_RS13930 0.58 -1.4 3,067,782 + LRK53_RS13930 0.58 -0.1 3,067,782 + LRK53_RS13930 0.58 +1.9 3,067,783 - LRK53_RS13930 0.59 -1.1 3,067,783 - LRK53_RS13930 0.59 -0.6 3,067,783 - LRK53_RS13930 0.59 +1.5 3,067,783 - LRK53_RS13930 0.59 +0.0 3,067,783 - LRK53_RS13930 0.59 -0.6 3,067,783 - LRK53_RS13930 0.59 -0.9 3,067,783 - LRK53_RS13930 0.59 -0.4 3,067,800 + LRK53_RS13930 0.61 -1.1 3,067,800 + LRK53_RS13930 0.61 -0.6 3,067,800 + LRK53_RS13930 0.61 +0.6 3,067,800 + LRK53_RS13930 0.61 +0.9 3,067,800 + LRK53_RS13930 0.61 -0.1 3,067,800 + LRK53_RS13930 0.61 -1.1 3,067,800 + LRK53_RS13930 0.61 -0.4 3,067,800 + LRK53_RS13930 0.61 -1.9 3,067,800 + LRK53_RS13930 0.61 -1.4 3,067,800 + LRK53_RS13930 0.61 -1.2 3,067,800 + LRK53_RS13930 0.61 -0.1 3,067,801 - LRK53_RS13930 0.62 -1.5 3,067,801 - LRK53_RS13930 0.62 -1.6 3,067,801 - LRK53_RS13930 0.62 -0.4 3,067,801 - LRK53_RS13930 0.62 -0.6 3,067,801 - LRK53_RS13930 0.62 -0.8 3,067,801 - LRK53_RS13930 0.62 -0.6 3,067,801 - LRK53_RS13930 0.62 +0.9 3,067,804 + LRK53_RS13930 0.62 -0.2 3,067,804 + LRK53_RS13930 0.62 -1.4 3,067,804 + LRK53_RS13930 0.62 -0.9 3,067,804 + LRK53_RS13930 0.62 -1.9 3,067,804 + LRK53_RS13930 0.62 -0.1 3,067,804 + LRK53_RS13930 0.62 -0.1 3,067,804 + LRK53_RS13930 0.62 +0.8 3,067,804 + LRK53_RS13930 0.62 +0.9 3,067,804 + LRK53_RS13930 0.62 -1.2 3,067,804 + LRK53_RS13930 0.62 +0.5 3,067,805 - LRK53_RS13930 0.62 +0.2 3,067,805 - LRK53_RS13930 0.62 -0.5 3,067,805 - LRK53_RS13930 0.62 -1.1 3,067,805 - LRK53_RS13930 0.62 -0.2 3,067,805 - LRK53_RS13930 0.62 -0.7 3,067,805 - LRK53_RS13930 0.62 +0.4 3,067,805 - LRK53_RS13930 0.62 -0.3 3,067,805 - LRK53_RS13930 0.62 +0.9 3,067,805 - LRK53_RS13930 0.62 -0.7 3,067,805 - LRK53_RS13930 0.62 -1.1 3,067,805 - LRK53_RS13930 0.62 -0.5 3,067,805 - LRK53_RS13930 0.62 -0.5 3,067,929 + LRK53_RS13930 0.83 -1.1 3,067,930 - LRK53_RS13930 0.84 -1.9 3,067,930 - LRK53_RS13930 0.84 +0.9 3,068,062 + -1.6 3,068,062 + +0.9 3,068,062 + +0.7 3,068,062 + +0.9 3,068,063 - +1.5 3,068,063 - +0.1 3,068,063 - -0.6 3,068,063 - +0.2 3,068,066 + -2.0 3,068,066 + +0.4 3,068,066 + +0.4 3,068,066 + -0.3 3,068,066 + -0.1 3,068,066 + +0.9 3,068,066 + +0.4 3,068,066 + -1.1 3,068,066 + -1.1 3,068,067 - +0.4 3,068,067 - -0.8 3,068,067 - +0.9 3,068,067 - -0.6 3,068,067 - -0.1 3,068,067 - -0.4 3,068,067 - -1.1 3,068,067 - -0.6 3,068,067 - -1.0 3,068,167 + -0.6 3,068,167 + +0.4 3,068,167 + -0.6 3,068,168 - -2.0 3,068,178 - -0.6 3,068,224 + -0.1 3,068,225 - +0.7 3,068,225 - +0.4 3,068,225 - -1.7 3,068,253 + -1.1 3,068,253 + -0.1 3,068,253 + -0.1 3,068,253 + -1.2 3,068,254 - -0.3 3,068,254 - -1.1 3,068,267 - -1.1 3,068,267 - -0.1
Or see this region's nucleotide sequence