Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07180 and LRK53_RS07185 are separated by 57 nucleotides LRK53_RS07185 and LRK53_RS07190 are separated by 157 nucleotides LRK53_RS07190 and LRK53_RS07195 are separated by 10 nucleotides LRK53_RS07195 and LRK53_RS07200 are separated by 127 nucleotides LRK53_RS07200 and LRK53_RS07205 are separated by 4 nucleotides
LRK53_RS07180: LRK53_RS07180 - DNA polymerase III subunit delta, at 1,617,281 to 1,618,291
_RS07180
LRK53_RS07185: LRK53_RS07185 - nicotinate-nucleotide adenylyltransferase, at 1,618,349 to 1,619,005
_RS07185
LRK53_RS07190: LRK53_RS07190 - PepSY domain-containing protein, at 1,619,163 to 1,620,731
_RS07190
LRK53_RS07195: LRK53_RS07195 - hypothetical protein, at 1,620,742 to 1,621,005
_RS07195
LRK53_RS07200: LRK53_RS07200 - hypothetical protein, at 1,621,133 to 1,621,432
_RS07200
LRK53_RS07205: LRK53_RS07205 - TonB-dependent siderophore receptor, at 1,621,437 to 1,623,599
_RS07205
Position (kb)
1619
1620
1621 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1618.341 kb on + strand at 1618.341 kb on + strand at 1618.341 kb on + strand at 1618.342 kb on - strand at 1618.342 kb on - strand at 1619.003 kb on + strand at 1619.003 kb on + strand at 1619.004 kb on - strand at 1619.004 kb on - strand at 1619.004 kb on - strand at 1619.040 kb on + strand at 1619.040 kb on + strand at 1619.040 kb on + strand at 1619.040 kb on + strand at 1619.040 kb on + strand at 1619.040 kb on + strand at 1619.040 kb on + strand at 1619.041 kb on - strand at 1619.041 kb on - strand at 1619.041 kb on - strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.067 kb on + strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.068 kb on - strand at 1619.165 kb on - strand at 1619.656 kb on + strand, within LRK53_RS07190 at 1619.657 kb on - strand, within LRK53_RS07190 at 1619.657 kb on - strand, within LRK53_RS07190 at 1619.692 kb on + strand, within LRK53_RS07190 at 1619.692 kb on + strand, within LRK53_RS07190 at 1619.692 kb on + strand, within LRK53_RS07190 at 1619.692 kb on + strand, within LRK53_RS07190 at 1619.693 kb on - strand, within LRK53_RS07190 at 1619.693 kb on - strand, within LRK53_RS07190 at 1619.693 kb on - strand, within LRK53_RS07190 at 1619.693 kb on - strand, within LRK53_RS07190 at 1619.693 kb on - strand, within LRK53_RS07190 at 1619.693 kb on - strand, within LRK53_RS07190 at 1619.719 kb on + strand, within LRK53_RS07190 at 1619.719 kb on + strand, within LRK53_RS07190 at 1619.720 kb on - strand, within LRK53_RS07190 at 1619.740 kb on + strand, within LRK53_RS07190 at 1619.740 kb on + strand, within LRK53_RS07190 at 1619.741 kb on - strand, within LRK53_RS07190 at 1619.741 kb on - strand, within LRK53_RS07190 at 1619.912 kb on - strand, within LRK53_RS07190 at 1619.912 kb on - strand, within LRK53_RS07190 at 1619.912 kb on - strand, within LRK53_RS07190 at 1619.912 kb on - strand, within LRK53_RS07190 at 1619.912 kb on - strand, within LRK53_RS07190 at 1619.912 kb on - strand, within LRK53_RS07190 at 1619.912 kb on - strand, within LRK53_RS07190 at 1620.070 kb on + strand, within LRK53_RS07190 at 1620.070 kb on + strand, within LRK53_RS07190 at 1620.071 kb on - strand, within LRK53_RS07190 at 1620.286 kb on + strand, within LRK53_RS07190 at 1620.287 kb on - strand, within LRK53_RS07190 at 1620.287 kb on - strand, within LRK53_RS07190 at 1620.287 kb on - strand, within LRK53_RS07190 at 1620.287 kb on - strand, within LRK53_RS07190 at 1620.287 kb on - strand, within LRK53_RS07190 at 1620.318 kb on - strand, within LRK53_RS07190 at 1620.343 kb on + strand, within LRK53_RS07190 at 1620.343 kb on + strand, within LRK53_RS07190 at 1620.343 kb on + strand, within LRK53_RS07190 at 1620.343 kb on + strand, within LRK53_RS07190 at 1620.343 kb on + strand, within LRK53_RS07190 at 1620.343 kb on + strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.344 kb on - strand, within LRK53_RS07190 at 1620.452 kb on - strand, within LRK53_RS07190 at 1620.452 kb on - strand, within LRK53_RS07190 at 1620.641 kb on - strand at 1621.109 kb on + strand at 1621.109 kb on + strand at 1621.109 kb on + strand at 1621.109 kb on + strand at 1621.109 kb on + strand at 1621.194 kb on + strand, within LRK53_RS07200 at 1621.195 kb on - strand, within LRK53_RS07200 at 1621.262 kb on + strand, within LRK53_RS07200 at 1621.263 kb on - strand, within LRK53_RS07200 at 1621.263 kb on - strand, within LRK53_RS07200 at 1621.263 kb on - strand, within LRK53_RS07200 at 1621.372 kb on - strand, within LRK53_RS07200 at 1621.372 kb on - strand, within LRK53_RS07200 at 1621.438 kb on + strand at 1621.478 kb on - strand at 1621.483 kb on + strand at 1621.483 kb on + strand at 1621.483 kb on + strand at 1621.483 kb on + strand at 1621.484 kb on - strand at 1621.486 kb on + strand at 1621.486 kb on + strand at 1621.486 kb on + strand at 1621.486 kb on + strand at 1621.486 kb on + strand at 1621.486 kb on + strand at 1621.486 kb on + strand at 1621.487 kb on - strand at 1621.487 kb on - strand at 1621.487 kb on - strand at 1621.487 kb on - strand at 1621.487 kb on - strand at 1621.495 kb on + strand at 1621.495 kb on + strand at 1621.495 kb on + strand at 1621.496 kb on - strand at 1621.518 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.519 kb on + strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.520 kb on - strand at 1621.576 kb on + strand at 1621.577 kb on - strand at 1621.603 kb on + strand at 1621.603 kb on + strand at 1621.603 kb on + strand at 1621.603 kb on + strand at 1621.603 kb on + strand at 1621.603 kb on + strand at 1621.603 kb on + strand at 1621.603 kb on + strand at 1621.604 kb on - strand at 1621.604 kb on - strand at 1621.604 kb on - strand at 1621.604 kb on - strand at 1621.604 kb on - strand at 1621.627 kb on + strand at 1621.627 kb on + strand at 1621.627 kb on + strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.628 kb on - strand at 1621.639 kb on + strand at 1621.639 kb on + strand at 1621.639 kb on + strand at 1621.654 kb on + strand, within LRK53_RS07205 at 1621.655 kb on - strand, within LRK53_RS07205 at 1621.655 kb on - strand, within LRK53_RS07205 at 1621.708 kb on + strand, within LRK53_RS07205 at 1621.708 kb on + strand, within LRK53_RS07205 at 1621.709 kb on - strand, within LRK53_RS07205 at 1621.709 kb on - strand, within LRK53_RS07205 at 1621.709 kb on - strand, within LRK53_RS07205 at 1621.709 kb on - strand, within LRK53_RS07205 at 1621.709 kb on - strand, within LRK53_RS07205 at 1621.709 kb on - strand, within LRK53_RS07205 at 1621.709 kb on - strand, within LRK53_RS07205
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 1,618,341 + -0.1 1,618,341 + -0.4 1,618,341 + +0.1 1,618,342 - +2.0 1,618,342 - -2.0 1,619,003 + +0.0 1,619,003 + +0.4 1,619,004 - -1.7 1,619,004 - -0.6 1,619,004 - -1.1 1,619,040 + -1.3 1,619,040 + -1.8 1,619,040 + -2.6 1,619,040 + +0.0 1,619,040 + -1.7 1,619,040 + -0.6 1,619,040 + -2.7 1,619,041 - +1.0 1,619,041 - +0.0 1,619,041 - +2.6 1,619,067 + -0.7 1,619,067 + -1.6 1,619,067 + +0.0 1,619,067 + -1.3 1,619,067 + +0.6 1,619,067 + -3.4 1,619,067 + -0.8 1,619,067 + -1.5 1,619,067 + -0.2 1,619,067 + -0.8 1,619,067 + -1.5 1,619,067 + +0.0 1,619,067 + +0.0 1,619,067 + -0.6 1,619,067 + +1.0 1,619,067 + -0.9 1,619,067 + +1.6 1,619,067 + +0.0 1,619,067 + -0.6 1,619,067 + -2.5 1,619,067 + -0.2 1,619,067 + +0.3 1,619,067 + +2.0 1,619,067 + -1.8 1,619,067 + +0.2 1,619,067 + -0.3 1,619,067 + +0.6 1,619,067 + -0.6 1,619,068 - -1.6 1,619,068 - -0.5 1,619,068 - -0.5 1,619,068 - -1.3 1,619,068 - -0.6 1,619,068 - +0.8 1,619,068 - +1.6 1,619,068 - -0.8 1,619,068 - -1.0 1,619,068 - +0.0 1,619,068 - -2.0 1,619,068 - -0.6 1,619,068 - -1.0 1,619,068 - -1.6 1,619,068 - -0.3 1,619,068 - -0.3 1,619,068 - +1.0 1,619,068 - -0.7 1,619,068 - +0.0 1,619,068 - +1.0 1,619,068 - +1.0 1,619,068 - +1.0 1,619,068 - -0.8 1,619,068 - -1.8 1,619,068 - +0.7 1,619,165 - -2.2 1,619,656 + LRK53_RS07190 0.31 +0.0 1,619,657 - LRK53_RS07190 0.31 -1.0 1,619,657 - LRK53_RS07190 0.31 -0.4 1,619,692 + LRK53_RS07190 0.34 -1.6 1,619,692 + LRK53_RS07190 0.34 -1.3 1,619,692 + LRK53_RS07190 0.34 +0.0 1,619,692 + LRK53_RS07190 0.34 +1.0 1,619,693 - LRK53_RS07190 0.34 +1.6 1,619,693 - LRK53_RS07190 0.34 +1.0 1,619,693 - LRK53_RS07190 0.34 -1.5 1,619,693 - LRK53_RS07190 0.34 +0.0 1,619,693 - LRK53_RS07190 0.34 -2.0 1,619,693 - LRK53_RS07190 0.34 -0.6 1,619,719 + LRK53_RS07190 0.35 +0.0 1,619,719 + LRK53_RS07190 0.35 -1.0 1,619,720 - LRK53_RS07190 0.36 +1.0 1,619,740 + LRK53_RS07190 0.37 -2.0 1,619,740 + LRK53_RS07190 0.37 -1.3 1,619,741 - LRK53_RS07190 0.37 +1.0 1,619,741 - LRK53_RS07190 0.37 -0.6 1,619,912 - LRK53_RS07190 0.48 -1.0 1,619,912 - LRK53_RS07190 0.48 -1.1 1,619,912 - LRK53_RS07190 0.48 -1.0 1,619,912 - LRK53_RS07190 0.48 -2.0 1,619,912 - LRK53_RS07190 0.48 -1.8 1,619,912 - LRK53_RS07190 0.48 -1.5 1,619,912 - LRK53_RS07190 0.48 -1.0 1,620,070 + LRK53_RS07190 0.58 +0.7 1,620,070 + LRK53_RS07190 0.58 -0.3 1,620,071 - LRK53_RS07190 0.58 +1.0 1,620,286 + LRK53_RS07190 0.72 -0.8 1,620,287 - LRK53_RS07190 0.72 +0.0 1,620,287 - LRK53_RS07190 0.72 -0.2 1,620,287 - LRK53_RS07190 0.72 -1.0 1,620,287 - LRK53_RS07190 0.72 -1.0 1,620,287 - LRK53_RS07190 0.72 -1.0 1,620,318 - LRK53_RS07190 0.74 -0.9 1,620,343 + LRK53_RS07190 0.75 -1.4 1,620,343 + LRK53_RS07190 0.75 -1.0 1,620,343 + LRK53_RS07190 0.75 -0.8 1,620,343 + LRK53_RS07190 0.75 -0.9 1,620,343 + LRK53_RS07190 0.75 +1.0 1,620,343 + LRK53_RS07190 0.75 -2.0 1,620,344 - LRK53_RS07190 0.75 +0.4 1,620,344 - LRK53_RS07190 0.75 -0.6 1,620,344 - LRK53_RS07190 0.75 -1.3 1,620,344 - LRK53_RS07190 0.75 +1.0 1,620,344 - LRK53_RS07190 0.75 -1.8 1,620,344 - LRK53_RS07190 0.75 -0.6 1,620,344 - LRK53_RS07190 0.75 -2.6 1,620,344 - LRK53_RS07190 0.75 -2.5 1,620,344 - LRK53_RS07190 0.75 -0.7 1,620,344 - LRK53_RS07190 0.75 +1.0 1,620,452 - LRK53_RS07190 0.82 -0.3 1,620,452 - LRK53_RS07190 0.82 -0.3 1,620,641 - -0.6 1,621,109 + -0.6 1,621,109 + -0.5 1,621,109 + +1.0 1,621,109 + -0.6 1,621,109 + -0.2 1,621,194 + LRK53_RS07200 0.20 -2.0 1,621,195 - LRK53_RS07200 0.21 -2.2 1,621,262 + LRK53_RS07200 0.43 -2.5 1,621,263 - LRK53_RS07200 0.43 -1.3 1,621,263 - LRK53_RS07200 0.43 +0.0 1,621,263 - LRK53_RS07200 0.43 -1.3 1,621,372 - LRK53_RS07200 0.80 +0.4 1,621,372 - LRK53_RS07200 0.80 +0.7 1,621,438 + -0.6 1,621,478 - +0.0 1,621,483 + -0.8 1,621,483 + -0.5 1,621,483 + -0.3 1,621,483 + -1.7 1,621,484 - -1.6 1,621,486 + +1.0 1,621,486 + -1.8 1,621,486 + +0.9 1,621,486 + -0.6 1,621,486 + +2.6 1,621,486 + -1.3 1,621,486 + -0.8 1,621,487 - +1.0 1,621,487 - +0.1 1,621,487 - -1.7 1,621,487 - -1.9 1,621,487 - +2.0 1,621,495 + -0.8 1,621,495 + -0.6 1,621,495 + -0.6 1,621,496 - +0.0 1,621,518 + -3.5 1,621,519 + -1.0 1,621,519 + +1.0 1,621,519 + +0.0 1,621,519 + -2.0 1,621,519 + -0.6 1,621,519 + -0.6 1,621,519 + +0.0 1,621,519 + -0.3 1,621,519 + -0.3 1,621,520 - -0.8 1,621,520 - -2.0 1,621,520 - +0.4 1,621,520 - +1.0 1,621,520 - +0.2 1,621,520 - -1.0 1,621,520 - -0.3 1,621,520 - +0.6 1,621,520 - -0.3 1,621,520 - -1.6 1,621,520 - +0.0 1,621,520 - -0.6 1,621,520 - +1.6 1,621,520 - -1.3 1,621,576 + -0.3 1,621,577 - -0.6 1,621,603 + +0.0 1,621,603 + -0.3 1,621,603 + -0.8 1,621,603 + -1.0 1,621,603 + -0.6 1,621,603 + -1.0 1,621,603 + +1.0 1,621,603 + -0.2 1,621,604 - +0.0 1,621,604 - -0.9 1,621,604 - -1.8 1,621,604 - -2.1 1,621,604 - -0.3 1,621,627 + +0.2 1,621,627 + -0.2 1,621,627 + +0.0 1,621,628 - +2.0 1,621,628 - -0.2 1,621,628 - +0.3 1,621,628 - +2.0 1,621,628 - +0.0 1,621,628 - -2.0 1,621,628 - -1.0 1,621,628 - -1.2 1,621,628 - +0.4 1,621,639 + -0.3 1,621,639 + -1.0 1,621,639 + +0.0 1,621,654 + LRK53_RS07205 0.10 -1.6 1,621,655 - LRK53_RS07205 0.10 +0.0 1,621,655 - LRK53_RS07205 0.10 +0.0 1,621,708 + LRK53_RS07205 0.13 -1.3 1,621,708 + LRK53_RS07205 0.13 -2.3 1,621,709 - LRK53_RS07205 0.13 +1.6 1,621,709 - LRK53_RS07205 0.13 +0.9 1,621,709 - LRK53_RS07205 0.13 -2.7 1,621,709 - LRK53_RS07205 0.13 -1.8 1,621,709 - LRK53_RS07205 0.13 +0.0 1,621,709 - LRK53_RS07205 0.13 -0.6 1,621,709 - LRK53_RS07205 0.13 -1.3
Or see this region's nucleotide sequence