Experiment: R2A_PIPES with Zinc chloride 5120 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04100 and LRK53_RS04105 are separated by 131 nucleotides LRK53_RS04105 and LRK53_RS04110 are separated by 0 nucleotides LRK53_RS04110 and LRK53_RS04115 are separated by 70 nucleotides LRK53_RS04115 and LRK53_RS04120 are separated by 62 nucleotides
LRK53_RS04100: LRK53_RS04100 - DUF3247 family protein, at 963,969 to 964,271
_RS04100
LRK53_RS04105: LRK53_RS04105 - FAD-dependent oxidoreductase, at 964,403 to 964,964
_RS04105
LRK53_RS04110: LRK53_RS04110 - LytTR family transcriptional regulator DNA-binding domain-containing protein, at 964,965 to 965,144
_RS04110
LRK53_RS04115: LRK53_RS04115 - hypothetical protein, at 965,215 to 965,949
_RS04115
LRK53_RS04120: LRK53_RS04120 - DUF6491 family protein, at 966,012 to 966,464
_RS04120
Position (kb) 964
965
966 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 963.993 kb on + strand at 963.993 kb on + strand at 963.994 kb on - strand at 963.994 kb on - strand at 963.994 kb on - strand at 963.994 kb on - strand at 964.043 kb on - strand, within LRK53_RS04100 at 964.043 kb on - strand, within LRK53_RS04100 at 964.043 kb on - strand, within LRK53_RS04100 at 964.043 kb on - strand, within LRK53_RS04100 at 964.043 kb on - strand, within LRK53_RS04100 at 964.043 kb on - strand, within LRK53_RS04100 at 964.043 kb on - strand, within LRK53_RS04100 at 964.043 kb on - strand, within LRK53_RS04100 at 964.455 kb on + strand at 964.455 kb on + strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.456 kb on - strand at 964.528 kb on - strand, within LRK53_RS04105 at 964.528 kb on - strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.545 kb on + strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.546 kb on - strand, within LRK53_RS04105 at 964.819 kb on + strand, within LRK53_RS04105 at 964.820 kb on - strand, within LRK53_RS04105 at 964.820 kb on - strand, within LRK53_RS04105 at 964.849 kb on + strand, within LRK53_RS04105 at 964.849 kb on + strand, within LRK53_RS04105 at 964.849 kb on + strand, within LRK53_RS04105 at 965.001 kb on + strand, within LRK53_RS04110 at 965.001 kb on + strand, within LRK53_RS04110 at 965.001 kb on + strand, within LRK53_RS04110 at 965.002 kb on - strand, within LRK53_RS04110 at 965.002 kb on - strand, within LRK53_RS04110 at 965.019 kb on + strand, within LRK53_RS04110 at 965.019 kb on + strand, within LRK53_RS04110 at 965.019 kb on + strand, within LRK53_RS04110 at 965.019 kb on + strand, within LRK53_RS04110 at 965.068 kb on + strand, within LRK53_RS04110 at 965.068 kb on + strand, within LRK53_RS04110 at 965.184 kb on + strand at 965.184 kb on + strand at 965.185 kb on - strand at 965.185 kb on - strand at 965.185 kb on - strand at 965.368 kb on + strand, within LRK53_RS04115 at 965.368 kb on + strand, within LRK53_RS04115 at 965.368 kb on + strand, within LRK53_RS04115 at 965.368 kb on + strand, within LRK53_RS04115 at 965.368 kb on + strand, within LRK53_RS04115 at 965.368 kb on + strand, within LRK53_RS04115 at 965.369 kb on - strand, within LRK53_RS04115 at 965.369 kb on - strand, within LRK53_RS04115 at 965.497 kb on + strand, within LRK53_RS04115 at 965.497 kb on + strand, within LRK53_RS04115 at 965.497 kb on + strand, within LRK53_RS04115 at 965.497 kb on + strand, within LRK53_RS04115 at 965.497 kb on + strand, within LRK53_RS04115 at 965.498 kb on - strand, within LRK53_RS04115 at 965.498 kb on - strand, within LRK53_RS04115 at 965.498 kb on - strand, within LRK53_RS04115 at 965.498 kb on - strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.542 kb on + strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.543 kb on - strand, within LRK53_RS04115 at 965.577 kb on + strand, within LRK53_RS04115 at 965.578 kb on - strand, within LRK53_RS04115 at 965.671 kb on + strand, within LRK53_RS04115 at 965.671 kb on + strand, within LRK53_RS04115 at 965.671 kb on + strand, within LRK53_RS04115 at 965.672 kb on - strand, within LRK53_RS04115 at 965.672 kb on - strand, within LRK53_RS04115 at 965.672 kb on - strand, within LRK53_RS04115 at 965.672 kb on - strand, within LRK53_RS04115 at 965.838 kb on + strand, within LRK53_RS04115 at 965.838 kb on + strand, within LRK53_RS04115 at 965.838 kb on + strand, within LRK53_RS04115 at 965.839 kb on - strand, within LRK53_RS04115 at 965.888 kb on - strand at 965.888 kb on - strand at 965.888 kb on - strand at 965.888 kb on - strand at 965.947 kb on + strand at 965.947 kb on + strand at 965.947 kb on + strand at 965.948 kb on - strand at 965.948 kb on - strand at 965.948 kb on - strand at 965.948 kb on - strand at 965.948 kb on - strand at 965.948 kb on - strand at 965.948 kb on - strand at 965.955 kb on + strand at 965.986 kb on + strand at 965.986 kb on + strand at 965.986 kb on + strand at 965.986 kb on + strand at 965.986 kb on + strand at 965.986 kb on + strand at 965.986 kb on + strand at 965.986 kb on + strand at 965.987 kb on - strand at 965.987 kb on - strand at 965.987 kb on - strand at 965.987 kb on - strand at 965.987 kb on - strand at 966.081 kb on + strand, within LRK53_RS04120 at 966.081 kb on + strand, within LRK53_RS04120 at 966.081 kb on + strand, within LRK53_RS04120 at 966.081 kb on + strand, within LRK53_RS04120 at 966.082 kb on - strand, within LRK53_RS04120 at 966.082 kb on - strand, within LRK53_RS04120 at 966.082 kb on - strand, within LRK53_RS04120 at 966.114 kb on + strand, within LRK53_RS04120 at 966.114 kb on + strand, within LRK53_RS04120 at 966.114 kb on + strand, within LRK53_RS04120 at 966.114 kb on + strand, within LRK53_RS04120 at 966.114 kb on + strand, within LRK53_RS04120 at 966.114 kb on + strand, within LRK53_RS04120 at 966.114 kb on + strand, within LRK53_RS04120 at 966.115 kb on - strand, within LRK53_RS04120 at 966.115 kb on - strand, within LRK53_RS04120 at 966.115 kb on - strand, within LRK53_RS04120 at 966.115 kb on - strand, within LRK53_RS04120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Zinc chloride 5120 uM remove 963,993 + +1.0 963,993 + -1.0 963,994 - -0.3 963,994 - +0.2 963,994 - -0.8 963,994 - +1.5 964,043 - LRK53_RS04100 0.24 +1.0 964,043 - LRK53_RS04100 0.24 -2.4 964,043 - LRK53_RS04100 0.24 -1.8 964,043 - LRK53_RS04100 0.24 -1.0 964,043 - LRK53_RS04100 0.24 +0.8 964,043 - LRK53_RS04100 0.24 -1.0 964,043 - LRK53_RS04100 0.24 -0.0 964,043 - LRK53_RS04100 0.24 -0.0 964,455 + -0.0 964,455 + -0.0 964,456 - -0.5 964,456 - -0.6 964,456 - -1.4 964,456 - -0.6 964,456 - -1.4 964,456 - -0.2 964,456 - +0.4 964,456 - -0.0 964,456 - -2.2 964,528 - LRK53_RS04105 0.22 -1.6 964,528 - LRK53_RS04105 0.22 -2.8 964,545 + LRK53_RS04105 0.25 -0.6 964,545 + LRK53_RS04105 0.25 -1.0 964,545 + LRK53_RS04105 0.25 -1.4 964,545 + LRK53_RS04105 0.25 -0.6 964,545 + LRK53_RS04105 0.25 +0.3 964,545 + LRK53_RS04105 0.25 -2.5 964,545 + LRK53_RS04105 0.25 +0.2 964,545 + LRK53_RS04105 0.25 -1.4 964,545 + LRK53_RS04105 0.25 -1.0 964,545 + LRK53_RS04105 0.25 +2.0 964,545 + LRK53_RS04105 0.25 -2.3 964,545 + LRK53_RS04105 0.25 -1.1 964,545 + LRK53_RS04105 0.25 -2.4 964,545 + LRK53_RS04105 0.25 -1.0 964,545 + LRK53_RS04105 0.25 -1.8 964,546 - LRK53_RS04105 0.25 -1.4 964,546 - LRK53_RS04105 0.25 -2.4 964,546 - LRK53_RS04105 0.25 -2.0 964,546 - LRK53_RS04105 0.25 -0.9 964,546 - LRK53_RS04105 0.25 -0.2 964,546 - LRK53_RS04105 0.25 -2.0 964,546 - LRK53_RS04105 0.25 +2.5 964,546 - LRK53_RS04105 0.25 +0.2 964,546 - LRK53_RS04105 0.25 -0.2 964,546 - LRK53_RS04105 0.25 -1.0 964,546 - LRK53_RS04105 0.25 +0.3 964,546 - LRK53_RS04105 0.25 -0.0 964,546 - LRK53_RS04105 0.25 -0.4 964,546 - LRK53_RS04105 0.25 -2.0 964,819 + LRK53_RS04105 0.74 +2.0 964,820 - LRK53_RS04105 0.74 -1.4 964,820 - LRK53_RS04105 0.74 +1.4 964,849 + LRK53_RS04105 0.79 -1.3 964,849 + LRK53_RS04105 0.79 +1.0 964,849 + LRK53_RS04105 0.79 -0.0 965,001 + LRK53_RS04110 0.20 -1.2 965,001 + LRK53_RS04110 0.20 -1.4 965,001 + LRK53_RS04110 0.20 -0.3 965,002 - LRK53_RS04110 0.21 -0.4 965,002 - LRK53_RS04110 0.21 +0.3 965,019 + LRK53_RS04110 0.30 -0.3 965,019 + LRK53_RS04110 0.30 +1.0 965,019 + LRK53_RS04110 0.30 +0.7 965,019 + LRK53_RS04110 0.30 -0.2 965,068 + LRK53_RS04110 0.57 -1.8 965,068 + LRK53_RS04110 0.57 -0.3 965,184 + +0.2 965,184 + +0.2 965,185 - +0.2 965,185 - -0.0 965,185 - -0.0 965,368 + LRK53_RS04115 0.21 +0.2 965,368 + LRK53_RS04115 0.21 -0.7 965,368 + LRK53_RS04115 0.21 -0.0 965,368 + LRK53_RS04115 0.21 -1.4 965,368 + LRK53_RS04115 0.21 -0.6 965,368 + LRK53_RS04115 0.21 -1.6 965,369 - LRK53_RS04115 0.21 -1.4 965,369 - LRK53_RS04115 0.21 -1.0 965,497 + LRK53_RS04115 0.38 -1.2 965,497 + LRK53_RS04115 0.38 -1.0 965,497 + LRK53_RS04115 0.38 +1.0 965,497 + LRK53_RS04115 0.38 -2.6 965,497 + LRK53_RS04115 0.38 -1.5 965,498 - LRK53_RS04115 0.39 -2.4 965,498 - LRK53_RS04115 0.39 +1.0 965,498 - LRK53_RS04115 0.39 +1.0 965,498 - LRK53_RS04115 0.39 -0.6 965,542 + LRK53_RS04115 0.44 -0.6 965,542 + LRK53_RS04115 0.44 -2.5 965,542 + LRK53_RS04115 0.44 -0.0 965,542 + LRK53_RS04115 0.44 -0.0 965,542 + LRK53_RS04115 0.44 -1.7 965,542 + LRK53_RS04115 0.44 +1.0 965,542 + LRK53_RS04115 0.44 -0.8 965,542 + LRK53_RS04115 0.44 -0.0 965,542 + LRK53_RS04115 0.44 -1.5 965,542 + LRK53_RS04115 0.44 -1.2 965,543 - LRK53_RS04115 0.45 -1.0 965,543 - LRK53_RS04115 0.45 -0.4 965,543 - LRK53_RS04115 0.45 -0.0 965,543 - LRK53_RS04115 0.45 -1.6 965,543 - LRK53_RS04115 0.45 -0.5 965,543 - LRK53_RS04115 0.45 -0.6 965,543 - LRK53_RS04115 0.45 -1.0 965,543 - LRK53_RS04115 0.45 -0.0 965,543 - LRK53_RS04115 0.45 -1.6 965,577 + LRK53_RS04115 0.49 -1.4 965,578 - LRK53_RS04115 0.49 -0.0 965,671 + LRK53_RS04115 0.62 -1.6 965,671 + LRK53_RS04115 0.62 -2.2 965,671 + LRK53_RS04115 0.62 -2.0 965,672 - LRK53_RS04115 0.62 -0.7 965,672 - LRK53_RS04115 0.62 -0.8 965,672 - LRK53_RS04115 0.62 -0.0 965,672 - LRK53_RS04115 0.62 -0.6 965,838 + LRK53_RS04115 0.85 -0.6 965,838 + LRK53_RS04115 0.85 +0.4 965,838 + LRK53_RS04115 0.85 -1.9 965,839 - LRK53_RS04115 0.85 -0.5 965,888 - -0.3 965,888 - -2.2 965,888 - -2.5 965,888 - -2.2 965,947 + +2.0 965,947 + -0.0 965,947 + -0.1 965,948 - -2.5 965,948 - +1.5 965,948 - -1.0 965,948 - -0.4 965,948 - -0.4 965,948 - -0.6 965,948 - -0.3 965,955 + +0.5 965,986 + -0.6 965,986 + +1.0 965,986 + +1.0 965,986 + -0.3 965,986 + +1.0 965,986 + +2.5 965,986 + -0.0 965,986 + -0.6 965,987 - +1.0 965,987 - -1.2 965,987 - -0.9 965,987 - -2.2 965,987 - +0.3 966,081 + LRK53_RS04120 0.15 +1.0 966,081 + LRK53_RS04120 0.15 -1.6 966,081 + LRK53_RS04120 0.15 -1.6 966,081 + LRK53_RS04120 0.15 -0.6 966,082 - LRK53_RS04120 0.15 -1.4 966,082 - LRK53_RS04120 0.15 -1.6 966,082 - LRK53_RS04120 0.15 +0.4 966,114 + LRK53_RS04120 0.23 +0.1 966,114 + LRK53_RS04120 0.23 +1.5 966,114 + LRK53_RS04120 0.23 -0.3 966,114 + LRK53_RS04120 0.23 -0.8 966,114 + LRK53_RS04120 0.23 -0.6 966,114 + LRK53_RS04120 0.23 -0.6 966,114 + LRK53_RS04120 0.23 -1.4 966,115 - LRK53_RS04120 0.23 -0.8 966,115 - LRK53_RS04120 0.23 -1.8 966,115 - LRK53_RS04120 0.23 +0.2 966,115 - LRK53_RS04120 0.23 +1.4
Or see this region's nucleotide sequence