Strain Fitness in Escherichia coli ECRC98 around JDDGAC_21130

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoA and JDDGAC_21120 are separated by 40 nucleotidesJDDGAC_21120 and yhdN are separated by 106 nucleotidesyhdN and zntR are separated by 10 nucleotideszntR and arfA are separated by 55 nucleotidesarfA and mscL overlap by 4 nucleotidesmscL and trkA are separated by 129 nucleotides JDDGAC_21115: rpoA - DNA-directed RNA polymerase subunit alpha, at 4,041,963 to 4,042,952 rpoA JDDGAC_21120: JDDGAC_21120 - 50S ribosomal protein L17, at 4,042,993 to 4,043,376 _21120 JDDGAC_21125: yhdN - Uncharacterized protein YhdN, at 4,043,483 to 4,043,851 yhdN JDDGAC_21130: zntR - Zn(2+)-responsive transcriptional regulator, at 4,043,862 to 4,044,287 zntR JDDGAC_21135: arfA - alternative ribosome-rescue factor ArfA, at 4,044,343 to 4,044,561 arfA JDDGAC_21140: mscL - large-conductance mechanosensitive channel protein MscL, at 4,044,558 to 4,044,968 mscL JDDGAC_21145: trkA - Trk system potassium transporter TrkA, at 4,045,098 to 4,046,474 trkA Position (kb) 4043 4044 4045Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4043.438 kb on + strandat 4043.439 kb on - strandat 4043.439 kb on - strandat 4043.439 kb on - strandat 4043.442 kb on + strandat 4043.443 kb on - strandat 4043.447 kb on + strandat 4043.448 kb on - strandat 4043.448 kb on - strandat 4043.478 kb on + strandat 4043.478 kb on + strandat 4043.482 kb on + strandat 4043.482 kb on + strandat 4043.482 kb on + strandat 4043.482 kb on + strandat 4043.483 kb on - strandat 4043.490 kb on + strandat 4043.491 kb on - strandat 4043.491 kb on - strandat 4043.516 kb on - strandat 4043.518 kb on - strandat 4043.518 kb on - strandat 4043.610 kb on + strand, within yhdNat 4043.757 kb on + strand, within yhdNat 4043.814 kb on - strand, within yhdNat 4043.845 kb on + strandat 4043.849 kb on + strandat 4043.849 kb on + strandat 4043.850 kb on - strandat 4043.857 kb on + strandat 4043.858 kb on - strandat 4043.858 kb on - strandat 4043.858 kb on - strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.861 kb on + strandat 4043.862 kb on - strandat 4043.862 kb on - strandat 4043.862 kb on - strandat 4043.862 kb on - strandat 4043.862 kb on - strandat 4043.862 kb on - strandat 4043.862 kb on - strandat 4043.900 kb on - strandat 4043.922 kb on + strand, within zntRat 4044.031 kb on + strand, within zntRat 4044.032 kb on - strand, within zntRat 4044.032 kb on - strand, within zntRat 4044.092 kb on + strand, within zntRat 4044.092 kb on + strand, within zntRat 4044.168 kb on - strand, within zntRat 4044.219 kb on - strand, within zntRat 4044.308 kb on - strandat 4044.360 kb on + strandat 4044.360 kb on + strandat 4044.361 kb on - strandat 4044.374 kb on + strand, within arfAat 4044.401 kb on + strand, within arfAat 4044.401 kb on + strand, within arfAat 4044.401 kb on + strand, within arfAat 4044.401 kb on + strand, within arfAat 4044.401 kb on + strand, within arfAat 4044.402 kb on - strand, within arfAat 4044.428 kb on + strand, within arfAat 4044.515 kb on + strand, within arfAat 4044.516 kb on - strand, within arfAat 4044.516 kb on - strand, within arfAat 4044.528 kb on + strand, within arfAat 4044.560 kb on - strandat 4044.560 kb on - strandat 4044.606 kb on + strand, within mscLat 4044.609 kb on + strand, within mscLat 4044.620 kb on + strand, within mscLat 4044.676 kb on - strand, within mscLat 4044.680 kb on + strand, within mscLat 4044.745 kb on + strand, within mscLat 4044.806 kb on + strand, within mscLat 4044.825 kb on + strand, within mscLat 4044.826 kb on - strand, within mscLat 4044.829 kb on - strand, within mscLat 4044.959 kb on + strandat 4044.972 kb on - strandat 4044.981 kb on - strandat 4044.997 kb on + strandat 4044.997 kb on + strandat 4044.998 kb on - strandat 4045.013 kb on + strandat 4045.096 kb on - strandat 4045.100 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,043,438 + +1.4
4,043,439 - +0.9
4,043,439 - -1.2
4,043,439 - +0.6
4,043,442 + +0.6
4,043,443 - -1.5
4,043,447 + +0.5
4,043,448 - -0.8
4,043,448 - +1.0
4,043,478 + +1.0
4,043,478 + -1.1
4,043,482 + +0.4
4,043,482 + -0.3
4,043,482 + +0.2
4,043,482 + -0.4
4,043,483 - -1.0
4,043,490 + +1.4
4,043,491 - -0.4
4,043,491 - -0.1
4,043,516 - -2.7
4,043,518 - -2.0
4,043,518 - +1.0
4,043,610 + yhdN JDDGAC_21125 0.34 -1.7
4,043,757 + yhdN JDDGAC_21125 0.74 -0.5
4,043,814 - yhdN JDDGAC_21125 0.90 -0.7
4,043,845 + +0.0
4,043,849 + -0.6
4,043,849 + +0.1
4,043,850 - -0.4
4,043,857 + +0.2
4,043,858 - +0.2
4,043,858 - +0.8
4,043,858 - +0.0
4,043,861 + +0.8
4,043,861 + -1.9
4,043,861 + +1.0
4,043,861 + +0.1
4,043,861 + -2.0
4,043,861 + +0.7
4,043,861 + -1.0
4,043,861 + -1.7
4,043,861 + -0.1
4,043,862 - +1.2
4,043,862 - +0.2
4,043,862 - -0.8
4,043,862 - -1.2
4,043,862 - +0.2
4,043,862 - +0.2
4,043,862 - +0.3
4,043,900 - +1.0
4,043,922 + zntR JDDGAC_21130 0.14 +0.7
4,044,031 + zntR JDDGAC_21130 0.40 +0.7
4,044,032 - zntR JDDGAC_21130 0.40 +0.4
4,044,032 - zntR JDDGAC_21130 0.40 +0.8
4,044,092 + zntR JDDGAC_21130 0.54 +1.7
4,044,092 + zntR JDDGAC_21130 0.54 -0.5
4,044,168 - zntR JDDGAC_21130 0.72 +1.3
4,044,219 - zntR JDDGAC_21130 0.84 +3.3
4,044,308 - +0.7
4,044,360 + -0.3
4,044,360 + +0.1
4,044,361 - +3.3
4,044,374 + arfA JDDGAC_21135 0.14 +1.1
4,044,401 + arfA JDDGAC_21135 0.26 -1.2
4,044,401 + arfA JDDGAC_21135 0.26 -2.7
4,044,401 + arfA JDDGAC_21135 0.26 -0.5
4,044,401 + arfA JDDGAC_21135 0.26 -0.7
4,044,401 + arfA JDDGAC_21135 0.26 -1.0
4,044,402 - arfA JDDGAC_21135 0.27 +0.8
4,044,428 + arfA JDDGAC_21135 0.39 -0.1
4,044,515 + arfA JDDGAC_21135 0.79 +0.0
4,044,516 - arfA JDDGAC_21135 0.79 -0.1
4,044,516 - arfA JDDGAC_21135 0.79 +1.2
4,044,528 + arfA JDDGAC_21135 0.84 +0.6
4,044,560 - -0.0
4,044,560 - +0.7
4,044,606 + mscL JDDGAC_21140 0.12 -2.0
4,044,609 + mscL JDDGAC_21140 0.12 -1.6
4,044,620 + mscL JDDGAC_21140 0.15 +0.2
4,044,676 - mscL JDDGAC_21140 0.29 -0.3
4,044,680 + mscL JDDGAC_21140 0.30 -0.5
4,044,745 + mscL JDDGAC_21140 0.45 +2.0
4,044,806 + mscL JDDGAC_21140 0.60 +1.1
4,044,825 + mscL JDDGAC_21140 0.65 +0.9
4,044,826 - mscL JDDGAC_21140 0.65 +0.8
4,044,829 - mscL JDDGAC_21140 0.66 -0.5
4,044,959 + +1.2
4,044,972 - -2.2
4,044,981 - +0.3
4,044,997 + +0.4
4,044,997 + -1.1
4,044,998 - +0.9
4,045,013 + +0.2
4,045,096 - -0.5
4,045,100 - -2.7

Or see this region's nucleotide sequence