Strain Fitness in Escherichia coli ECRC98 around JDDGAC_19310

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaB and rfaL are separated by 35 nucleotidesrfaL and rfaC overlap by 4 nucleotidesrfaC and rfaF are separated by 3 nucleotides JDDGAC_19305: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 3,671,822 to 3,672,964 rfaB JDDGAC_19310: rfaL - O-antigen ligase RfaL, at 3,673,000 to 3,674,208 rfaL JDDGAC_19315: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 3,674,205 to 3,675,197 rfaC JDDGAC_19320: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 3,675,201 to 3,676,247 rfaF Position (kb) 3672 3673 3674 3675Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7 8 9at 3672.072 kb on - strand, within rfaBat 3672.198 kb on + strand, within rfaBat 3672.386 kb on - strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.602 kb on - strand, within rfaBat 3672.607 kb on - strand, within rfaBat 3672.712 kb on + strand, within rfaBat 3672.779 kb on - strand, within rfaBat 3672.806 kb on - strand, within rfaBat 3672.905 kb on + strandat 3672.962 kb on + strandat 3673.189 kb on + strand, within rfaLat 3673.216 kb on + strand, within rfaLat 3673.261 kb on + strand, within rfaLat 3673.272 kb on + strand, within rfaLat 3673.305 kb on + strand, within rfaLat 3673.323 kb on - strand, within rfaLat 3673.437 kb on - strand, within rfaLat 3673.977 kb on + strand, within rfaLat 3673.981 kb on + strand, within rfaLat 3674.085 kb on + strand, within rfaLat 3674.239 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,672,072 - rfaB JDDGAC_19305 0.22 +9.3
3,672,198 + rfaB JDDGAC_19305 0.33 +7.2
3,672,386 - rfaB JDDGAC_19305 0.49 +6.0
3,672,601 + rfaB JDDGAC_19305 0.68 +6.8
3,672,601 + rfaB JDDGAC_19305 0.68 +7.6
3,672,601 + rfaB JDDGAC_19305 0.68 +8.1
3,672,602 - rfaB JDDGAC_19305 0.68 +6.6
3,672,607 - rfaB JDDGAC_19305 0.69 +8.9
3,672,712 + rfaB JDDGAC_19305 0.78 +6.6
3,672,779 - rfaB JDDGAC_19305 0.84 +7.9
3,672,806 - rfaB JDDGAC_19305 0.86 +6.6
3,672,905 + +7.2
3,672,962 + -0.3
3,673,189 + rfaL JDDGAC_19310 0.16 +5.6
3,673,216 + rfaL JDDGAC_19310 0.18 +6.4
3,673,261 + rfaL JDDGAC_19310 0.22 +6.3
3,673,272 + rfaL JDDGAC_19310 0.22 +6.1
3,673,305 + rfaL JDDGAC_19310 0.25 +6.3
3,673,323 - rfaL JDDGAC_19310 0.27 +6.5
3,673,437 - rfaL JDDGAC_19310 0.36 +6.6
3,673,977 + rfaL JDDGAC_19310 0.81 +6.9
3,673,981 + rfaL JDDGAC_19310 0.81 +5.2
3,674,085 + rfaL JDDGAC_19310 0.90 +8.1
3,674,239 - +0.7

Or see this region's nucleotide sequence