Strain Fitness in Escherichia coli ECRC98 around JDDGAC_15905

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmiaA and mutL overlap by 8 nucleotidesmutL and amiB are separated by 9 nucleotides JDDGAC_15900: miaA - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 2,991,945 to 2,992,895 miaA JDDGAC_15905: mutL - DNA mismatch repair endonuclease MutL, at 2,992,888 to 2,994,735 mutL JDDGAC_15910: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 2,994,745 to 2,996,082 amiB Position (kb) 2992 2993 2994 2995Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7at 2991.926 kb on - strandat 2991.926 kb on - strandat 2991.926 kb on - strandat 2991.926 kb on - strandat 2991.957 kb on - strandat 2991.957 kb on - strandat 2991.957 kb on - strandat 2991.976 kb on - strandat 2992.048 kb on - strand, within miaAat 2992.509 kb on + strand, within miaAat 2992.510 kb on - strand, within miaAat 2992.757 kb on - strand, within miaAat 2992.910 kb on - strandat 2992.921 kb on + strandat 2992.922 kb on - strandat 2992.937 kb on - strandat 2993.002 kb on + strandat 2993.098 kb on + strand, within mutLat 2993.099 kb on - strand, within mutLat 2993.099 kb on - strand, within mutLat 2993.099 kb on - strand, within mutLat 2993.102 kb on + strand, within mutLat 2993.327 kb on - strand, within mutLat 2993.362 kb on + strand, within mutLat 2993.362 kb on + strand, within mutLat 2993.610 kb on - strand, within mutLat 2993.610 kb on - strand, within mutLat 2993.620 kb on + strand, within mutLat 2993.747 kb on - strand, within mutLat 2993.768 kb on + strand, within mutLat 2993.769 kb on - strand, within mutLat 2993.769 kb on - strand, within mutLat 2993.772 kb on + strand, within mutLat 2993.773 kb on - strand, within mutLat 2993.864 kb on + strand, within mutLat 2993.865 kb on - strand, within mutLat 2993.865 kb on - strand, within mutLat 2993.966 kb on + strand, within mutLat 2993.966 kb on + strand, within mutLat 2993.967 kb on - strand, within mutLat 2993.973 kb on - strand, within mutLat 2994.226 kb on - strand, within mutLat 2994.231 kb on + strand, within mutLat 2994.281 kb on + strand, within mutLat 2994.281 kb on + strand, within mutLat 2994.281 kb on + strand, within mutLat 2994.282 kb on - strand, within mutLat 2994.282 kb on - strand, within mutLat 2994.295 kb on + strand, within mutLat 2994.365 kb on + strand, within mutLat 2994.469 kb on - strand, within mutLat 2994.607 kb on - strandat 2994.658 kb on + strandat 2994.840 kb on - strandat 2994.857 kb on + strandat 2994.857 kb on + strandat 2994.857 kb on + strandat 2995.005 kb on + strand, within amiBat 2995.005 kb on + strand, within amiBat 2995.006 kb on - strand, within amiBat 2995.006 kb on - strand, within amiBat 2995.041 kb on + strand, within amiBat 2995.041 kb on + strand, within amiBat 2995.058 kb on + strand, within amiBat 2995.059 kb on - strand, within amiBat 2995.087 kb on + strand, within amiBat 2995.108 kb on + strand, within amiBat 2995.112 kb on + strand, within amiBat 2995.112 kb on + strand, within amiBat 2995.309 kb on - strand, within amiBat 2995.341 kb on + strand, within amiBat 2995.346 kb on + strand, within amiBat 2995.347 kb on - strand, within amiBat 2995.364 kb on - strand, within amiBat 2995.374 kb on + strand, within amiBat 2995.401 kb on + strand, within amiBat 2995.513 kb on - strand, within amiBat 2995.560 kb on + strand, within amiBat 2995.560 kb on + strand, within amiBat 2995.560 kb on + strand, within amiBat 2995.561 kb on - strand, within amiBat 2995.579 kb on - strand, within amiBat 2995.587 kb on + strand, within amiBat 2995.600 kb on - strand, within amiBat 2995.644 kb on + strand, within amiBat 2995.645 kb on - strand, within amiBat 2995.727 kb on + strand, within amiBat 2995.727 kb on + strand, within amiBat 2995.727 kb on + strand, within amiBat 2995.728 kb on - strand, within amiBat 2995.728 kb on - strand, within amiB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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2,991,926 - -0.6
2,991,926 - -0.1
2,991,926 - -2.0
2,991,926 - -1.8
2,991,957 - -0.1
2,991,957 - +0.6
2,991,957 - -1.0
2,991,976 - +0.1
2,992,048 - miaA JDDGAC_15900 0.11 -0.4
2,992,509 + miaA JDDGAC_15900 0.59 +0.0
2,992,510 - miaA JDDGAC_15900 0.59 -0.6
2,992,757 - miaA JDDGAC_15900 0.85 +1.0
2,992,910 - -0.6
2,992,921 + -1.0
2,992,922 - +1.3
2,992,937 - +2.3
2,993,002 + +2.8
2,993,098 + mutL JDDGAC_15905 0.11 +1.6
2,993,099 - mutL JDDGAC_15905 0.11 -0.4
2,993,099 - mutL JDDGAC_15905 0.11 +1.0
2,993,099 - mutL JDDGAC_15905 0.11 +2.7
2,993,102 + mutL JDDGAC_15905 0.12 -0.8
2,993,327 - mutL JDDGAC_15905 0.24 +7.7
2,993,362 + mutL JDDGAC_15905 0.26 +1.5
2,993,362 + mutL JDDGAC_15905 0.26 -0.2
2,993,610 - mutL JDDGAC_15905 0.39 -1.2
2,993,610 - mutL JDDGAC_15905 0.39 +0.4
2,993,620 + mutL JDDGAC_15905 0.40 -0.8
2,993,747 - mutL JDDGAC_15905 0.46 +0.5
2,993,768 + mutL JDDGAC_15905 0.48 +0.1
2,993,769 - mutL JDDGAC_15905 0.48 +3.2
2,993,769 - mutL JDDGAC_15905 0.48 +3.2
2,993,772 + mutL JDDGAC_15905 0.48 +1.2
2,993,773 - mutL JDDGAC_15905 0.48 +2.5
2,993,864 + mutL JDDGAC_15905 0.53 -0.6
2,993,865 - mutL JDDGAC_15905 0.53 -0.2
2,993,865 - mutL JDDGAC_15905 0.53 +2.1
2,993,966 + mutL JDDGAC_15905 0.58 +0.6
2,993,966 + mutL JDDGAC_15905 0.58 +0.7
2,993,967 - mutL JDDGAC_15905 0.58 +1.7
2,993,973 - mutL JDDGAC_15905 0.59 +3.5
2,994,226 - mutL JDDGAC_15905 0.72 +2.2
2,994,231 + mutL JDDGAC_15905 0.73 +0.8
2,994,281 + mutL JDDGAC_15905 0.75 -1.4
2,994,281 + mutL JDDGAC_15905 0.75 +1.5
2,994,281 + mutL JDDGAC_15905 0.75 +0.3
2,994,282 - mutL JDDGAC_15905 0.75 -1.1
2,994,282 - mutL JDDGAC_15905 0.75 +0.2
2,994,295 + mutL JDDGAC_15905 0.76 -0.1
2,994,365 + mutL JDDGAC_15905 0.80 -1.6
2,994,469 - mutL JDDGAC_15905 0.86 -0.8
2,994,607 - -2.0
2,994,658 + +1.3
2,994,840 - +0.1
2,994,857 + -0.2
2,994,857 + +0.6
2,994,857 + -2.7
2,995,005 + amiB JDDGAC_15910 0.19 -0.5
2,995,005 + amiB JDDGAC_15910 0.19 -1.0
2,995,006 - amiB JDDGAC_15910 0.20 +0.7
2,995,006 - amiB JDDGAC_15910 0.20 -0.4
2,995,041 + amiB JDDGAC_15910 0.22 +1.5
2,995,041 + amiB JDDGAC_15910 0.22 +2.2
2,995,058 + amiB JDDGAC_15910 0.23 +0.4
2,995,059 - amiB JDDGAC_15910 0.23 +1.3
2,995,087 + amiB JDDGAC_15910 0.26 +0.5
2,995,108 + amiB JDDGAC_15910 0.27 -0.5
2,995,112 + amiB JDDGAC_15910 0.27 -1.4
2,995,112 + amiB JDDGAC_15910 0.27 +0.3
2,995,309 - amiB JDDGAC_15910 0.42 +0.0
2,995,341 + amiB JDDGAC_15910 0.45 -0.4
2,995,346 + amiB JDDGAC_15910 0.45 -0.8
2,995,347 - amiB JDDGAC_15910 0.45 -0.2
2,995,364 - amiB JDDGAC_15910 0.46 -0.5
2,995,374 + amiB JDDGAC_15910 0.47 +0.5
2,995,401 + amiB JDDGAC_15910 0.49 -0.3
2,995,513 - amiB JDDGAC_15910 0.57 -1.5
2,995,560 + amiB JDDGAC_15910 0.61 +1.4
2,995,560 + amiB JDDGAC_15910 0.61 +0.0
2,995,560 + amiB JDDGAC_15910 0.61 +1.0
2,995,561 - amiB JDDGAC_15910 0.61 -0.1
2,995,579 - amiB JDDGAC_15910 0.62 -0.2
2,995,587 + amiB JDDGAC_15910 0.63 -1.0
2,995,600 - amiB JDDGAC_15910 0.64 +0.1
2,995,644 + amiB JDDGAC_15910 0.67 +0.5
2,995,645 - amiB JDDGAC_15910 0.67 -0.3
2,995,727 + amiB JDDGAC_15910 0.73 -0.3
2,995,727 + amiB JDDGAC_15910 0.73 -0.3
2,995,727 + amiB JDDGAC_15910 0.73 +0.9
2,995,728 - amiB JDDGAC_15910 0.73 -2.5
2,995,728 - amiB JDDGAC_15910 0.73 -1.1

Or see this region's nucleotide sequence