Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolA and tolR are separated by 64 nucleotides tolR and tolQ are separated by 3 nucleotides tolQ and ybgC overlap by 4 nucleotides ybgC and ybgE are separated by 149 nucleotides ybgE and cydX overlap by 1 nucleotides cydX and cydB are separated by 14 nucleotides
JDDGAC_10465: tolA - cell envelope integrity protein TolA, at 1,863,282 to 1,864,496
tolA
JDDGAC_10470: tolR - colicin uptake protein TolR, at 1,864,561 to 1,864,989
tolR
JDDGAC_10475: tolQ - Tol-Pal system protein TolQ, at 1,864,993 to 1,865,685
tolQ
JDDGAC_10480: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 1,865,682 to 1,866,086
ybgC
JDDGAC_10485: ybgE - cyd operon protein YbgE, at 1,866,236 to 1,866,529
ybgE
JDDGAC_10490: cydX - cytochrome bd-I oxidase subunit CydX, at 1,866,529 to 1,866,642
cydX
JDDGAC_10495: cydB - cytochrome d ubiquinol oxidase subunit II, at 1,866,657 to 1,867,796
cydB
Position (kb)
1864
1865
1866 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1864.422 kb on + strand at 1864.449 kb on - strand at 1864.453 kb on - strand at 1864.562 kb on + strand at 1864.563 kb on - strand at 1864.566 kb on + strand at 1864.567 kb on - strand at 1864.567 kb on - strand at 1864.609 kb on + strand, within tolR at 1864.609 kb on + strand, within tolR at 1864.626 kb on + strand, within tolR at 1864.770 kb on - strand, within tolR at 1864.918 kb on + strand, within tolR at 1864.933 kb on + strand, within tolR at 1864.934 kb on - strand, within tolR at 1865.111 kb on + strand, within tolQ at 1865.270 kb on + strand, within tolQ at 1865.271 kb on - strand, within tolQ at 1865.271 kb on - strand, within tolQ at 1865.346 kb on + strand, within tolQ at 1865.802 kb on - strand, within ybgC at 1865.842 kb on - strand, within ybgC at 1865.854 kb on - strand, within ybgC at 1865.907 kb on - strand, within ybgC at 1865.907 kb on - strand, within ybgC at 1866.004 kb on + strand, within ybgC at 1866.005 kb on - strand, within ybgC at 1866.046 kb on + strand at 1866.046 kb on + strand at 1866.047 kb on - strand at 1866.047 kb on - strand at 1866.049 kb on + strand at 1866.080 kb on + strand at 1866.081 kb on - strand at 1866.090 kb on - strand at 1866.114 kb on + strand at 1866.133 kb on + strand at 1866.137 kb on - strand at 1866.138 kb on + strand at 1866.436 kb on + strand, within ybgE at 1866.505 kb on - strand at 1866.505 kb on - strand at 1866.523 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 1,864,422 + +2.5 1,864,449 - +0.2 1,864,453 - +0.3 1,864,562 + +0.6 1,864,563 - -0.7 1,864,566 + -1.1 1,864,567 - -0.2 1,864,567 - +0.9 1,864,609 + tolR JDDGAC_10470 0.11 +0.3 1,864,609 + tolR JDDGAC_10470 0.11 +1.9 1,864,626 + tolR JDDGAC_10470 0.15 +0.1 1,864,770 - tolR JDDGAC_10470 0.49 +1.9 1,864,918 + tolR JDDGAC_10470 0.83 +1.8 1,864,933 + tolR JDDGAC_10470 0.87 +0.9 1,864,934 - tolR JDDGAC_10470 0.87 -0.1 1,865,111 + tolQ JDDGAC_10475 0.17 -0.5 1,865,270 + tolQ JDDGAC_10475 0.40 -1.4 1,865,271 - tolQ JDDGAC_10475 0.40 +0.9 1,865,271 - tolQ JDDGAC_10475 0.40 -2.0 1,865,346 + tolQ JDDGAC_10475 0.51 +2.5 1,865,802 - ybgC JDDGAC_10480 0.30 +0.9 1,865,842 - ybgC JDDGAC_10480 0.40 -0.4 1,865,854 - ybgC JDDGAC_10480 0.42 +0.3 1,865,907 - ybgC JDDGAC_10480 0.56 -2.2 1,865,907 - ybgC JDDGAC_10480 0.56 +0.2 1,866,004 + ybgC JDDGAC_10480 0.80 -0.2 1,866,005 - ybgC JDDGAC_10480 0.80 -0.4 1,866,046 + +0.9 1,866,046 + -0.0 1,866,047 - -0.5 1,866,047 - +1.1 1,866,049 + -0.7 1,866,080 + -0.7 1,866,081 - -1.1 1,866,090 - +0.8 1,866,114 + +0.6 1,866,133 + -0.5 1,866,137 - +1.3 1,866,138 + -1.4 1,866,436 + ybgE JDDGAC_10485 0.68 +1.9 1,866,505 - -2.0 1,866,505 - -0.7 1,866,523 + +0.3
Or see this region's nucleotide sequence