Strain Fitness in Escherichia coli ECRC98 around JDDGAC_10030

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbioD and bioC overlap by 8 nucleotidesbioC and bioF overlap by 14 nucleotidesbioF and bioB overlap by 4 nucleotides JDDGAC_10020: bioD - dethiobiotin synthase, at 1,788,690 to 1,789,367 bioD JDDGAC_10025: bioC - malonyl-ACP O-methyltransferase BioC, at 1,789,360 to 1,790,115 bioC JDDGAC_10030: bioF - 8-amino-7-oxononanoate synthase, at 1,790,102 to 1,791,256 bioF JDDGAC_10035: bioB - biotin synthase BioB, at 1,791,253 to 1,792,293 bioB Position (kb) 1790 1791 1792Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7 8at 1789.145 kb on - strand, within bioDat 1789.191 kb on + strand, within bioDat 1789.208 kb on + strand, within bioDat 1789.215 kb on + strand, within bioDat 1789.215 kb on + strand, within bioDat 1789.216 kb on - strand, within bioDat 1789.296 kb on + strand, within bioDat 1789.296 kb on + strand, within bioDat 1789.394 kb on + strandat 1789.394 kb on + strandat 1789.394 kb on + strandat 1789.466 kb on - strand, within bioCat 1789.618 kb on + strand, within bioCat 1789.630 kb on - strand, within bioCat 1789.759 kb on + strand, within bioCat 1789.906 kb on + strand, within bioCat 1789.978 kb on + strand, within bioCat 1789.978 kb on + strand, within bioCat 1789.992 kb on + strand, within bioCat 1789.992 kb on + strand, within bioCat 1789.992 kb on + strand, within bioCat 1790.057 kb on + strandat 1790.057 kb on + strandat 1790.057 kb on + strandat 1790.058 kb on - strandat 1790.100 kb on + strandat 1790.430 kb on - strand, within bioFat 1790.467 kb on - strand, within bioFat 1790.470 kb on + strand, within bioFat 1790.497 kb on - strand, within bioFat 1790.678 kb on + strand, within bioFat 1790.864 kb on - strand, within bioFat 1790.941 kb on + strand, within bioFat 1790.942 kb on - strand, within bioFat 1790.946 kb on - strand, within bioFat 1791.010 kb on + strand, within bioFat 1791.010 kb on + strand, within bioFat 1791.108 kb on - strand, within bioFat 1791.135 kb on + strand, within bioFat 1791.256 kb on + strandat 1791.256 kb on + strandat 1791.273 kb on - strandat 1791.273 kb on - strandat 1791.273 kb on - strandat 1791.396 kb on + strand, within bioBat 1791.522 kb on - strand, within bioBat 1791.606 kb on + strand, within bioBat 1791.607 kb on - strand, within bioBat 1791.709 kb on + strand, within bioBat 1791.709 kb on + strand, within bioBat 1791.710 kb on - strand, within bioBat 1791.710 kb on - strand, within bioBat 1791.768 kb on + strand, within bioBat 1791.786 kb on - strand, within bioBat 1791.789 kb on + strand, within bioBat 1791.790 kb on - strand, within bioBat 1791.909 kb on + strand, within bioBat 1791.909 kb on + strand, within bioBat 1791.910 kb on - strand, within bioBat 1791.960 kb on - strand, within bioBat 1792.118 kb on + strand, within bioBat 1792.250 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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1,789,145 - bioD JDDGAC_10020 0.67 +0.2
1,789,191 + bioD JDDGAC_10020 0.74 -0.5
1,789,208 + bioD JDDGAC_10020 0.76 +0.1
1,789,215 + bioD JDDGAC_10020 0.77 +2.3
1,789,215 + bioD JDDGAC_10020 0.77 -0.5
1,789,216 - bioD JDDGAC_10020 0.78 +0.4
1,789,296 + bioD JDDGAC_10020 0.89 +2.0
1,789,296 + bioD JDDGAC_10020 0.89 +2.5
1,789,394 + +0.6
1,789,394 + +0.1
1,789,394 + -2.2
1,789,466 - bioC JDDGAC_10025 0.14 +0.1
1,789,618 + bioC JDDGAC_10025 0.34 -2.4
1,789,630 - bioC JDDGAC_10025 0.36 -0.2
1,789,759 + bioC JDDGAC_10025 0.53 -0.7
1,789,906 + bioC JDDGAC_10025 0.72 -3.2
1,789,978 + bioC JDDGAC_10025 0.82 +0.8
1,789,978 + bioC JDDGAC_10025 0.82 -1.6
1,789,992 + bioC JDDGAC_10025 0.84 -2.5
1,789,992 + bioC JDDGAC_10025 0.84 -0.6
1,789,992 + bioC JDDGAC_10025 0.84 -0.8
1,790,057 + +0.7
1,790,057 + +0.0
1,790,057 + +0.7
1,790,058 - -0.8
1,790,100 + -0.3
1,790,430 - bioF JDDGAC_10030 0.28 -0.1
1,790,467 - bioF JDDGAC_10030 0.32 +0.5
1,790,470 + bioF JDDGAC_10030 0.32 -0.1
1,790,497 - bioF JDDGAC_10030 0.34 -0.5
1,790,678 + bioF JDDGAC_10030 0.50 -0.5
1,790,864 - bioF JDDGAC_10030 0.66 -0.6
1,790,941 + bioF JDDGAC_10030 0.73 -0.4
1,790,942 - bioF JDDGAC_10030 0.73 +8.3
1,790,946 - bioF JDDGAC_10030 0.73 +1.5
1,791,010 + bioF JDDGAC_10030 0.79 +0.4
1,791,010 + bioF JDDGAC_10030 0.79 -0.4
1,791,108 - bioF JDDGAC_10030 0.87 -2.8
1,791,135 + bioF JDDGAC_10030 0.89 -1.4
1,791,256 + +1.6
1,791,256 + +1.2
1,791,273 - -1.2
1,791,273 - +0.2
1,791,273 - +0.3
1,791,396 + bioB JDDGAC_10035 0.14 +0.6
1,791,522 - bioB JDDGAC_10035 0.26 +0.3
1,791,606 + bioB JDDGAC_10035 0.34 -0.1
1,791,607 - bioB JDDGAC_10035 0.34 +0.8
1,791,709 + bioB JDDGAC_10035 0.44 -1.7
1,791,709 + bioB JDDGAC_10035 0.44 +0.0
1,791,710 - bioB JDDGAC_10035 0.44 -0.8
1,791,710 - bioB JDDGAC_10035 0.44 +0.6
1,791,768 + bioB JDDGAC_10035 0.49 +0.4
1,791,786 - bioB JDDGAC_10035 0.51 +2.9
1,791,789 + bioB JDDGAC_10035 0.51 -0.5
1,791,790 - bioB JDDGAC_10035 0.52 -0.3
1,791,909 + bioB JDDGAC_10035 0.63 +0.3
1,791,909 + bioB JDDGAC_10035 0.63 +2.5
1,791,910 - bioB JDDGAC_10035 0.63 -0.5
1,791,960 - bioB JDDGAC_10035 0.68 +2.5
1,792,118 + bioB JDDGAC_10035 0.83 +0.1
1,792,250 - -0.3

Or see this region's nucleotide sequence