Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinG and espX2 are separated by 283 nucleotides espX2 and rhlE are separated by 607 nucleotides
JDDGAC_09915: dinG - ATP-dependent DNA helicase DinG, at 1,764,343 to 1,766,493
dinG
JDDGAC_09920: espX2 - type III secretion system effector EspX2, at 1,766,777 to 1,768,789
espX2
JDDGAC_09925: rhlE - ATP-dependent RNA helicase RhlE, at 1,769,397 to 1,770,764
rhlE
Position (kb)
1766
1767
1768
1769 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1765.892 kb on + strand, within dinG at 1765.892 kb on + strand, within dinG at 1765.893 kb on - strand, within dinG at 1765.896 kb on - strand, within dinG at 1765.957 kb on + strand, within dinG at 1765.971 kb on + strand, within dinG at 1765.995 kb on + strand, within dinG at 1765.995 kb on + strand, within dinG at 1765.996 kb on - strand, within dinG at 1765.996 kb on - strand, within dinG at 1765.996 kb on - strand, within dinG at 1765.998 kb on - strand, within dinG at 1765.998 kb on - strand, within dinG at 1765.998 kb on - strand, within dinG at 1765.998 kb on - strand, within dinG at 1766.050 kb on - strand, within dinG at 1766.050 kb on - strand, within dinG at 1766.216 kb on + strand, within dinG at 1766.451 kb on - strand at 1766.473 kb on - strand at 1766.473 kb on - strand at 1766.509 kb on - strand at 1766.513 kb on + strand at 1766.608 kb on - strand at 1766.686 kb on + strand at 1766.855 kb on + strand at 1767.914 kb on + strand, within espX2 at 1768.079 kb on + strand, within espX2 at 1768.115 kb on - strand, within espX2 at 1769.012 kb on - strand at 1769.375 kb on - strand at 1769.395 kb on + strand at 1769.398 kb on + strand at 1769.780 kb on - strand, within rhlE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 1,765,892 + dinG JDDGAC_09915 0.72 +3.8 1,765,892 + dinG JDDGAC_09915 0.72 +1.5 1,765,893 - dinG JDDGAC_09915 0.72 -0.1 1,765,896 - dinG JDDGAC_09915 0.72 -2.5 1,765,957 + dinG JDDGAC_09915 0.75 +1.8 1,765,971 + dinG JDDGAC_09915 0.76 -1.0 1,765,995 + dinG JDDGAC_09915 0.77 +1.3 1,765,995 + dinG JDDGAC_09915 0.77 -0.1 1,765,996 - dinG JDDGAC_09915 0.77 -1.5 1,765,996 - dinG JDDGAC_09915 0.77 +0.0 1,765,996 - dinG JDDGAC_09915 0.77 +0.6 1,765,998 - dinG JDDGAC_09915 0.77 -0.3 1,765,998 - dinG JDDGAC_09915 0.77 -0.6 1,765,998 - dinG JDDGAC_09915 0.77 -0.9 1,765,998 - dinG JDDGAC_09915 0.77 -0.3 1,766,050 - dinG JDDGAC_09915 0.79 -0.2 1,766,050 - dinG JDDGAC_09915 0.79 +1.7 1,766,216 + dinG JDDGAC_09915 0.87 -1.8 1,766,451 - -0.3 1,766,473 - -0.3 1,766,473 - -0.3 1,766,509 - -1.1 1,766,513 + -0.4 1,766,608 - -1.4 1,766,686 + -0.7 1,766,855 + +0.8 1,767,914 + espX2 JDDGAC_09920 0.56 -0.3 1,768,079 + espX2 JDDGAC_09920 0.65 +1.1 1,768,115 - espX2 JDDGAC_09920 0.66 +1.7 1,769,012 - +0.3 1,769,375 - +1.1 1,769,395 + +1.6 1,769,398 + -1.8 1,769,780 - rhlE JDDGAC_09925 0.28 -0.5
Or see this region's nucleotide sequence