Strain Fitness in Escherichia coli ECRC98 around JDDGAC_08140

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcadC1 and JDDGAC_08135 are separated by 4 nucleotidesJDDGAC_08135 and eAL are separated by 101 nucleotideseAL and dgcT are separated by 97 nucleotides JDDGAC_08130: cadC1 - transcriptional regulator, at 1,416,448 to 1,417,260 cadC1 JDDGAC_08135: JDDGAC_08135 - FidL, at 1,417,265 to 1,417,756 _08135 JDDGAC_08140: eAL - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 1,417,858 to 1,419,468 eAL JDDGAC_08145: dgcT - putative diguanylate cyclase DgcT, at 1,419,566 to 1,420,924 dgcT Position (kb) 1417 1418 1419 1420Strain fitness (log2 ratio) -2 -1 0 1 2at 1417.088 kb on + strand, within cadC1at 1417.707 kb on - strandat 1417.710 kb on + strandat 1418.087 kb on - strand, within eALat 1418.998 kb on + strand, within eALat 1419.199 kb on - strand, within eALat 1419.347 kb on - strandat 1419.373 kb on - strandat 1419.528 kb on - strandat 1419.611 kb on - strandat 1419.637 kb on - strandat 1419.739 kb on - strand, within dgcTat 1420.177 kb on - strand, within dgcTat 1420.292 kb on + strand, within dgcT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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1,417,088 + cadC1 JDDGAC_08130 0.79 +0.7
1,417,707 - +1.6
1,417,710 + +1.6
1,418,087 - eAL JDDGAC_08140 0.14 -0.2
1,418,998 + eAL JDDGAC_08140 0.71 -1.7
1,419,199 - eAL JDDGAC_08140 0.83 -1.7
1,419,347 - -2.2
1,419,373 - -0.5
1,419,528 - -0.8
1,419,611 - +2.1
1,419,637 - +2.2
1,419,739 - dgcT JDDGAC_08145 0.13 -1.7
1,420,177 - dgcT JDDGAC_08145 0.45 +1.0
1,420,292 + dgcT JDDGAC_08145 0.53 -0.7

Or see this region's nucleotide sequence