Strain Fitness in Escherichia coli ECRC98 around JDDGAC_07100

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpotD and cobB are separated by 155 nucleotidescobB and nagK are separated by 15 nucleotidesnagK and lolE are separated by 28 nucleotides JDDGAC_07095: potD - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 1,224,247 to 1,225,293 potD JDDGAC_07100: cobB - NAD-dependent protein deacylase, at 1,225,449 to 1,226,270 cobB JDDGAC_07105: nagK - N-acetylglucosamine kinase, at 1,226,286 to 1,227,197 nagK JDDGAC_07110: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 1,227,226 to 1,228,464 lolE Position (kb) 1225 1226 1227Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1224.460 kb on + strand, within potDat 1224.499 kb on + strand, within potDat 1224.499 kb on + strand, within potDat 1224.502 kb on + strand, within potDat 1224.502 kb on + strand, within potDat 1224.503 kb on - strand, within potDat 1224.619 kb on + strand, within potDat 1224.632 kb on - strand, within potDat 1224.633 kb on + strand, within potDat 1224.780 kb on + strand, within potDat 1224.780 kb on + strand, within potDat 1224.834 kb on + strand, within potDat 1224.834 kb on + strand, within potDat 1224.835 kb on - strand, within potDat 1224.898 kb on + strand, within potDat 1224.898 kb on + strand, within potDat 1224.898 kb on + strand, within potDat 1224.898 kb on + strand, within potDat 1224.915 kb on + strand, within potDat 1224.972 kb on + strand, within potDat 1225.264 kb on + strandat 1225.264 kb on + strandat 1225.292 kb on - strandat 1225.451 kb on - strandat 1225.452 kb on + strandat 1225.668 kb on - strand, within cobBat 1225.669 kb on + strand, within cobBat 1225.708 kb on - strand, within cobBat 1225.755 kb on - strand, within cobBat 1225.767 kb on - strand, within cobBat 1225.851 kb on - strand, within cobBat 1226.143 kb on - strand, within cobBat 1226.288 kb on - strandat 1226.296 kb on - strandat 1226.371 kb on - strandat 1226.481 kb on - strand, within nagKat 1226.503 kb on - strand, within nagKat 1226.503 kb on - strand, within nagKat 1226.841 kb on - strand, within nagKat 1226.899 kb on - strand, within nagKat 1226.972 kb on - strand, within nagKat 1227.194 kb on - strandat 1227.218 kb on + strandat 1227.228 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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1,224,460 + potD JDDGAC_07095 0.20 +0.6
1,224,499 + potD JDDGAC_07095 0.24 -0.1
1,224,499 + potD JDDGAC_07095 0.24 -0.6
1,224,502 + potD JDDGAC_07095 0.24 +0.4
1,224,502 + potD JDDGAC_07095 0.24 -0.4
1,224,503 - potD JDDGAC_07095 0.24 +2.4
1,224,619 + potD JDDGAC_07095 0.36 -1.9
1,224,632 - potD JDDGAC_07095 0.37 -0.1
1,224,633 + potD JDDGAC_07095 0.37 +2.0
1,224,780 + potD JDDGAC_07095 0.51 -0.1
1,224,780 + potD JDDGAC_07095 0.51 -1.3
1,224,834 + potD JDDGAC_07095 0.56 -1.0
1,224,834 + potD JDDGAC_07095 0.56 +2.4
1,224,835 - potD JDDGAC_07095 0.56 +1.4
1,224,898 + potD JDDGAC_07095 0.62 -0.9
1,224,898 + potD JDDGAC_07095 0.62 +0.0
1,224,898 + potD JDDGAC_07095 0.62 +1.8
1,224,898 + potD JDDGAC_07095 0.62 +1.1
1,224,915 + potD JDDGAC_07095 0.64 -1.0
1,224,972 + potD JDDGAC_07095 0.69 +0.5
1,225,264 + -1.1
1,225,264 + -0.1
1,225,292 - +0.6
1,225,451 - -0.6
1,225,452 + +1.6
1,225,668 - cobB JDDGAC_07100 0.27 +0.1
1,225,669 + cobB JDDGAC_07100 0.27 +0.8
1,225,708 - cobB JDDGAC_07100 0.32 +3.1
1,225,755 - cobB JDDGAC_07100 0.37 +2.2
1,225,767 - cobB JDDGAC_07100 0.39 +0.7
1,225,851 - cobB JDDGAC_07100 0.49 -0.6
1,226,143 - cobB JDDGAC_07100 0.84 +0.2
1,226,288 - -2.5
1,226,296 - -0.4
1,226,371 - -2.2
1,226,481 - nagK JDDGAC_07105 0.21 +0.1
1,226,503 - nagK JDDGAC_07105 0.24 -0.8
1,226,503 - nagK JDDGAC_07105 0.24 -0.6
1,226,841 - nagK JDDGAC_07105 0.61 +0.4
1,226,899 - nagK JDDGAC_07105 0.67 +2.1
1,226,972 - nagK JDDGAC_07105 0.75 +2.6
1,227,194 - -1.9
1,227,218 + +0.9
1,227,228 - +1.3

Or see this region's nucleotide sequence