Strain Fitness in Escherichia coli ECRC98 around JDDGAC_05080

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdeR and yciZ are separated by 147 nucleotidesyciZ and yciT are separated by 89 nucleotidesyciT and osmB are separated by 268 nucleotidesosmB and yciH are separated by 125 nucleotidesyciH and pyrF overlap by 1 nucleotides JDDGAC_05070: pdeR - cyclic di-GMP phosphodiesterase, at 890,461 to 892,446 pdeR JDDGAC_05075: yciZ - UPF0509 protein YciZ, at 892,594 to 892,767 yciZ JDDGAC_05080: yciT - DNA-binding transcriptional regulator YciT, at 892,857 to 893,606 yciT JDDGAC_05085: osmB - osmotically-inducible lipoprotein OsmB, at 893,875 to 894,093 osmB JDDGAC_05090: yciH - stress response translation initiation inhibitor YciH, at 894,219 to 894,545 yciH JDDGAC_05095: pyrF - orotidine-5'-phosphate decarboxylase, at 894,545 to 895,342 pyrF Position (kb) 892 893 894Strain fitness (log2 ratio) -2 -1 0 1 2 3at 892.456 kb on + strandat 892.456 kb on + strandat 892.456 kb on + strandat 892.461 kb on - strandat 892.486 kb on - strandat 892.487 kb on + strandat 892.578 kb on + strandat 892.698 kb on + strand, within yciZat 892.836 kb on - strandat 893.065 kb on - strand, within yciTat 893.067 kb on - strand, within yciTat 893.291 kb on + strand, within yciTat 893.291 kb on + strand, within yciTat 893.354 kb on + strand, within yciTat 893.354 kb on + strand, within yciTat 893.576 kb on + strandat 893.816 kb on + strandat 893.825 kb on + strandat 894.216 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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892,456 + -1.1
892,456 + +0.4
892,456 + -0.6
892,461 - +0.8
892,486 - -2.0
892,487 + +2.1
892,578 + +0.6
892,698 + yciZ JDDGAC_05075 0.60 +0.5
892,836 - +1.4
893,065 - yciT JDDGAC_05080 0.28 +0.3
893,067 - yciT JDDGAC_05080 0.28 +0.6
893,291 + yciT JDDGAC_05080 0.58 +1.0
893,291 + yciT JDDGAC_05080 0.58 +1.9
893,354 + yciT JDDGAC_05080 0.66 +1.1
893,354 + yciT JDDGAC_05080 0.66 +3.6
893,576 + -0.2
893,816 + +1.6
893,825 + -0.8
894,216 - +0.2

Or see this region's nucleotide sequence