Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt puuC and puuR are separated by 149 nucleotides puuR and puuD are separated by 26 nucleotides puuD and puuA are separated by 211 nucleotides
JDDGAC_04975: puuC - aldehyde dehydrogenase PuuC, at 865,877 to 867,364
puuC
JDDGAC_04980: puuR - HTH-type transcriptional regulator PuuR, at 867,514 to 868,071
puuR
JDDGAC_04985: puuD - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 868,098 to 868,850
puuD
JDDGAC_04990: puuA - glutamate-putrescine ligase, at 869,062 to 870,480
puuA
Position (kb) 868
869 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9 at 867.116 kb on - strand, within puuC at 867.332 kb on - strand at 867.392 kb on + strand at 867.393 kb on - strand at 867.394 kb on + strand at 867.395 kb on - strand at 867.395 kb on - strand at 867.395 kb on - strand at 867.436 kb on + strand at 867.556 kb on - strand at 867.567 kb on + strand at 867.568 kb on - strand at 867.568 kb on - strand at 867.597 kb on + strand, within puuR at 867.680 kb on - strand, within puuR at 867.832 kb on - strand, within puuR at 867.897 kb on - strand, within puuR at 867.897 kb on - strand, within puuR at 867.942 kb on + strand, within puuR at 867.952 kb on + strand, within puuR at 868.072 kb on - strand at 868.092 kb on + strand at 868.093 kb on - strand at 868.252 kb on - strand, within puuD at 868.629 kb on + strand, within puuD at 868.629 kb on + strand, within puuD at 868.629 kb on + strand, within puuD at 868.630 kb on - strand, within puuD at 868.630 kb on - strand, within puuD at 868.701 kb on + strand, within puuD at 868.702 kb on - strand, within puuD at 868.813 kb on - strand at 868.897 kb on - strand at 868.930 kb on - strand at 869.177 kb on - strand at 869.355 kb on + strand, within puuA at 869.549 kb on - strand, within puuA at 869.649 kb on + strand, within puuA at 869.703 kb on - strand, within puuA at 869.773 kb on - strand, within puuA at 869.773 kb on - strand, within puuA at 869.779 kb on - strand, within puuA at 869.847 kb on + strand, within puuA at 869.847 kb on + strand, within puuA at 869.847 kb on + strand, within puuA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 867,116 - puuC JDDGAC_04975 0.83 -0.4 867,332 - +0.5 867,392 + +0.2 867,393 - -0.4 867,394 + -1.0 867,395 - +0.6 867,395 - -0.3 867,395 - +1.1 867,436 + -0.1 867,556 - -1.2 867,567 + +9.6 867,568 - -0.5 867,568 - +3.7 867,597 + puuR JDDGAC_04980 0.15 +0.2 867,680 - puuR JDDGAC_04980 0.30 +1.0 867,832 - puuR JDDGAC_04980 0.57 +1.7 867,897 - puuR JDDGAC_04980 0.69 -0.0 867,897 - puuR JDDGAC_04980 0.69 +0.2 867,942 + puuR JDDGAC_04980 0.77 +1.7 867,952 + puuR JDDGAC_04980 0.78 -0.1 868,072 - -0.8 868,092 + -1.1 868,093 - +0.5 868,252 - puuD JDDGAC_04985 0.20 +2.8 868,629 + puuD JDDGAC_04985 0.71 +1.0 868,629 + puuD JDDGAC_04985 0.71 +1.7 868,629 + puuD JDDGAC_04985 0.71 +1.4 868,630 - puuD JDDGAC_04985 0.71 -0.5 868,630 - puuD JDDGAC_04985 0.71 +1.7 868,701 + puuD JDDGAC_04985 0.80 +3.7 868,702 - puuD JDDGAC_04985 0.80 +0.1 868,813 - -0.0 868,897 - -1.4 868,930 - +0.6 869,177 - -0.9 869,355 + puuA JDDGAC_04990 0.21 -1.6 869,549 - puuA JDDGAC_04990 0.34 +0.8 869,649 + puuA JDDGAC_04990 0.41 +0.1 869,703 - puuA JDDGAC_04990 0.45 -1.2 869,773 - puuA JDDGAC_04990 0.50 +1.8 869,773 - puuA JDDGAC_04990 0.50 +2.6 869,779 - puuA JDDGAC_04990 0.51 +1.1 869,847 + puuA JDDGAC_04990 0.55 -1.6 869,847 + puuA JDDGAC_04990 0.55 -0.3 869,847 + puuA JDDGAC_04990 0.55 -2.6
Or see this region's nucleotide sequence