Strain Fitness in Escherichia coli ECRC98 around JDDGAC_00745

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhB and flhA overlap by 8 nucleotidesflhA and flhe overlap by 4 nucleotidesflhe and yecT are separated by 119 nucleotides JDDGAC_00740: flhB - flagellar biosynthesis protein FlhB, at 121,118 to 122,266 flhB JDDGAC_00745: flhA - flagellar biosynthesis protein FlhA, at 122,259 to 124,337 flhA JDDGAC_00750: flhe - flagellar protein FlhE, at 124,334 to 124,729 flhe JDDGAC_00755: yecT - Uncharacterized protein YecT, at 124,849 to 125,337 yecT Position (kb) 122 123 124 125Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 121.324 kb on + strand, within flhBat 121.515 kb on + strand, within flhBat 121.555 kb on + strand, within flhBat 121.624 kb on + strand, within flhBat 121.635 kb on + strand, within flhBat 121.689 kb on + strand, within flhBat 121.834 kb on + strand, within flhBat 122.199 kb on + strandat 122.206 kb on + strandat 122.264 kb on + strandat 122.264 kb on + strandat 122.386 kb on + strandat 122.522 kb on + strand, within flhAat 122.522 kb on + strand, within flhAat 122.522 kb on + strand, within flhAat 122.864 kb on + strand, within flhAat 122.865 kb on - strand, within flhAat 122.865 kb on - strand, within flhAat 122.984 kb on + strand, within flhAat 122.984 kb on + strand, within flhAat 123.296 kb on + strand, within flhAat 123.296 kb on + strand, within flhAat 123.328 kb on + strand, within flhAat 123.328 kb on + strand, within flhAat 123.328 kb on + strand, within flhAat 123.328 kb on + strand, within flhAat 123.329 kb on - strand, within flhAat 123.417 kb on - strand, within flhAat 123.489 kb on - strand, within flhAat 123.538 kb on + strand, within flhAat 123.605 kb on - strand, within flhAat 123.648 kb on - strand, within flhAat 123.721 kb on + strand, within flhAat 123.735 kb on - strand, within flhAat 123.873 kb on - strand, within flhAat 124.265 kb on - strandat 124.348 kb on - strandat 124.356 kb on + strandat 124.411 kb on + strand, within flheat 124.412 kb on - strand, within flheat 124.428 kb on + strand, within flheat 124.938 kb on + strand, within yecTat 125.168 kb on + strand, within yecTat 125.168 kb on + strand, within yecTat 125.271 kb on + strand, within yecT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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121,324 + flhB JDDGAC_00740 0.18 -1.5
121,515 + flhB JDDGAC_00740 0.35 -1.2
121,555 + flhB JDDGAC_00740 0.38 +0.6
121,624 + flhB JDDGAC_00740 0.44 -0.6
121,635 + flhB JDDGAC_00740 0.45 -0.1
121,689 + flhB JDDGAC_00740 0.50 -0.9
121,834 + flhB JDDGAC_00740 0.62 -1.7
122,199 + -1.1
122,206 + -0.2
122,264 + -1.1
122,264 + -1.3
122,386 + +1.1
122,522 + flhA JDDGAC_00745 0.13 +1.3
122,522 + flhA JDDGAC_00745 0.13 +0.9
122,522 + flhA JDDGAC_00745 0.13 +0.1
122,864 + flhA JDDGAC_00745 0.29 -1.4
122,865 - flhA JDDGAC_00745 0.29 +1.3
122,865 - flhA JDDGAC_00745 0.29 +0.6
122,984 + flhA JDDGAC_00745 0.35 +0.6
122,984 + flhA JDDGAC_00745 0.35 +2.0
123,296 + flhA JDDGAC_00745 0.50 +1.4
123,296 + flhA JDDGAC_00745 0.50 +1.2
123,328 + flhA JDDGAC_00745 0.51 -1.0
123,328 + flhA JDDGAC_00745 0.51 +1.6
123,328 + flhA JDDGAC_00745 0.51 -1.6
123,328 + flhA JDDGAC_00745 0.51 +6.0
123,329 - flhA JDDGAC_00745 0.51 +3.0
123,417 - flhA JDDGAC_00745 0.56 +0.7
123,489 - flhA JDDGAC_00745 0.59 -1.2
123,538 + flhA JDDGAC_00745 0.62 +1.3
123,605 - flhA JDDGAC_00745 0.65 +0.3
123,648 - flhA JDDGAC_00745 0.67 +1.3
123,721 + flhA JDDGAC_00745 0.70 -0.7
123,735 - flhA JDDGAC_00745 0.71 +0.1
123,873 - flhA JDDGAC_00745 0.78 +1.3
124,265 - -0.5
124,348 - +2.6
124,356 + -0.4
124,411 + flhe JDDGAC_00750 0.19 +0.6
124,412 - flhe JDDGAC_00750 0.20 +2.0
124,428 + flhe JDDGAC_00750 0.24 +2.6
124,938 + yecT JDDGAC_00755 0.18 +1.8
125,168 + yecT JDDGAC_00755 0.65 +0.8
125,168 + yecT JDDGAC_00755 0.65 +0.4
125,271 + yecT JDDGAC_00755 0.86 -0.6

Or see this region's nucleotide sequence