Experiment: R2A_PIPES with Nickel (II) chloride 848 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18170 and LRK53_RS18175 are separated by 81 nucleotides LRK53_RS18175 and LRK53_RS18180 are separated by 288 nucleotides
LRK53_RS18170: LRK53_RS18170 - HAMP domain-containing histidine kinase, at 8,472 to 9,839
_RS18170
LRK53_RS18175: LRK53_RS18175 - DEAD/DEAH box helicase, at 9,921 to 13,061
_RS18175
LRK53_RS18180: LRK53_RS18180 - WGR domain-containing protein, at 13,350 to 14,474
_RS18180
Position (kb)
9
10
11
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 9.219 kb on + strand, within LRK53_RS18170 at 9.220 kb on - strand, within LRK53_RS18170 at 9.220 kb on - strand, within LRK53_RS18170 at 9.292 kb on - strand, within LRK53_RS18170 at 9.494 kb on - strand, within LRK53_RS18170 at 9.494 kb on - strand, within LRK53_RS18170 at 9.539 kb on - strand, within LRK53_RS18170 at 9.621 kb on + strand, within LRK53_RS18170 at 9.671 kb on - strand, within LRK53_RS18170 at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.833 kb on + strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 9.834 kb on - strand at 10.161 kb on - strand at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.449 kb on + strand, within LRK53_RS18175 at 10.450 kb on - strand, within LRK53_RS18175 at 10.450 kb on - strand, within LRK53_RS18175 at 10.450 kb on - strand, within LRK53_RS18175 at 10.450 kb on - strand, within LRK53_RS18175 at 10.450 kb on - strand, within LRK53_RS18175 at 10.450 kb on - strand, within LRK53_RS18175 at 10.856 kb on + strand, within LRK53_RS18175 at 10.857 kb on - strand, within LRK53_RS18175 at 10.977 kb on + strand, within LRK53_RS18175 at 10.978 kb on - strand, within LRK53_RS18175 at 10.978 kb on - strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.271 kb on + strand, within LRK53_RS18175 at 11.272 kb on - strand, within LRK53_RS18175 at 11.272 kb on - strand, within LRK53_RS18175 at 11.272 kb on - strand, within LRK53_RS18175 at 11.272 kb on - strand, within LRK53_RS18175 at 11.272 kb on - strand, within LRK53_RS18175 at 11.272 kb on - strand, within LRK53_RS18175 at 11.475 kb on + strand, within LRK53_RS18175 at 11.475 kb on + strand, within LRK53_RS18175 at 11.475 kb on + strand, within LRK53_RS18175 at 11.476 kb on - strand, within LRK53_RS18175 at 11.476 kb on - strand, within LRK53_RS18175 at 11.476 kb on - strand, within LRK53_RS18175 at 11.476 kb on - strand, within LRK53_RS18175 at 11.629 kb on + strand, within LRK53_RS18175 at 11.629 kb on + strand, within LRK53_RS18175 at 11.630 kb on - strand, within LRK53_RS18175 at 11.630 kb on - strand, within LRK53_RS18175 at 11.670 kb on + strand, within LRK53_RS18175 at 11.670 kb on + strand, within LRK53_RS18175 at 11.670 kb on + strand, within LRK53_RS18175 at 11.671 kb on - strand, within LRK53_RS18175 at 11.671 kb on - strand, within LRK53_RS18175 at 11.707 kb on + strand, within LRK53_RS18175 at 11.707 kb on + strand, within LRK53_RS18175 at 11.708 kb on - strand, within LRK53_RS18175 at 11.708 kb on - strand, within LRK53_RS18175 at 11.708 kb on - strand, within LRK53_RS18175 at 11.730 kb on + strand, within LRK53_RS18175 at 11.778 kb on + strand, within LRK53_RS18175 at 11.778 kb on + strand, within LRK53_RS18175 at 11.871 kb on + strand, within LRK53_RS18175 at 11.871 kb on + strand, within LRK53_RS18175 at 11.871 kb on + strand, within LRK53_RS18175 at 11.871 kb on + strand, within LRK53_RS18175 at 11.871 kb on + strand, within LRK53_RS18175 at 11.872 kb on - strand, within LRK53_RS18175 at 11.872 kb on - strand, within LRK53_RS18175 at 11.872 kb on - strand, within LRK53_RS18175 at 11.872 kb on - strand, within LRK53_RS18175 at 11.872 kb on - strand, within LRK53_RS18175 at 11.988 kb on + strand, within LRK53_RS18175 at 12.006 kb on + strand, within LRK53_RS18175 at 12.007 kb on - strand, within LRK53_RS18175 at 12.007 kb on - strand, within LRK53_RS18175 at 12.007 kb on - strand, within LRK53_RS18175 at 12.205 kb on + strand, within LRK53_RS18175 at 12.205 kb on + strand, within LRK53_RS18175 at 12.205 kb on + strand, within LRK53_RS18175 at 12.231 kb on + strand, within LRK53_RS18175 at 12.231 kb on + strand, within LRK53_RS18175 at 12.231 kb on + strand, within LRK53_RS18175 at 12.231 kb on + strand, within LRK53_RS18175 at 12.231 kb on + strand, within LRK53_RS18175 at 12.232 kb on - strand, within LRK53_RS18175 at 12.232 kb on - strand, within LRK53_RS18175 at 12.232 kb on - strand, within LRK53_RS18175 at 12.232 kb on - strand, within LRK53_RS18175 at 12.232 kb on - strand, within LRK53_RS18175 at 12.298 kb on - strand, within LRK53_RS18175 at 12.430 kb on - strand, within LRK53_RS18175 at 12.462 kb on + strand, within LRK53_RS18175 at 12.462 kb on + strand, within LRK53_RS18175 at 12.462 kb on + strand, within LRK53_RS18175 at 12.463 kb on - strand, within LRK53_RS18175 at 12.463 kb on - strand, within LRK53_RS18175 at 12.463 kb on - strand, within LRK53_RS18175 at 12.463 kb on - strand, within LRK53_RS18175 at 12.489 kb on + strand, within LRK53_RS18175 at 12.489 kb on + strand, within LRK53_RS18175 at 12.543 kb on + strand, within LRK53_RS18175 at 12.543 kb on + strand, within LRK53_RS18175 at 12.543 kb on + strand, within LRK53_RS18175 at 12.543 kb on + strand, within LRK53_RS18175 at 12.543 kb on + strand, within LRK53_RS18175 at 12.544 kb on - strand, within LRK53_RS18175 at 12.544 kb on - strand, within LRK53_RS18175 at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.873 kb on + strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 12.874 kb on - strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.180 kb on + strand at 13.181 kb on - strand at 13.181 kb on - strand at 13.181 kb on - strand at 13.181 kb on - strand at 13.181 kb on - strand at 13.181 kb on - strand at 13.181 kb on - strand at 13.219 kb on + strand at 13.220 kb on - strand at 13.220 kb on - strand at 13.220 kb on - strand at 13.262 kb on + strand at 13.262 kb on + strand at 13.263 kb on - strand at 13.263 kb on - strand at 13.263 kb on - strand at 13.263 kb on - strand at 13.263 kb on - strand at 13.263 kb on - strand at 13.277 kb on - strand at 13.386 kb on + strand at 13.386 kb on + strand at 13.386 kb on + strand at 13.387 kb on - strand at 13.387 kb on - strand at 13.387 kb on - strand at 13.387 kb on - strand at 13.410 kb on + strand at 13.449 kb on + strand at 13.449 kb on + strand at 13.449 kb on + strand at 13.449 kb on + strand at 13.449 kb on + strand at 13.449 kb on + strand at 13.450 kb on - strand at 13.450 kb on - strand at 13.450 kb on - strand at 13.450 kb on - strand at 13.450 kb on - strand at 13.468 kb on - strand, within LRK53_RS18180 at 13.516 kb on + strand, within LRK53_RS18180 at 13.516 kb on + strand, within LRK53_RS18180 at 13.517 kb on - strand, within LRK53_RS18180 at 13.578 kb on + strand, within LRK53_RS18180 at 13.578 kb on + strand, within LRK53_RS18180 at 13.579 kb on - strand, within LRK53_RS18180 at 13.579 kb on - strand, within LRK53_RS18180 at 13.926 kb on + strand, within LRK53_RS18180 at 13.927 kb on - strand, within LRK53_RS18180 at 13.927 kb on - strand, within LRK53_RS18180 at 13.927 kb on - strand, within LRK53_RS18180 at 14.033 kb on - strand, within LRK53_RS18180 at 14.049 kb on + strand, within LRK53_RS18180 at 14.049 kb on + strand, within LRK53_RS18180 at 14.050 kb on - strand, within LRK53_RS18180 at 14.050 kb on - strand, within LRK53_RS18180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 848 uM remove 9,219 + LRK53_RS18170 0.55 -1.4 9,220 - LRK53_RS18170 0.55 -0.1 9,220 - LRK53_RS18170 0.55 -3.7 9,292 - LRK53_RS18170 0.60 -1.4 9,494 - LRK53_RS18170 0.75 +0.1 9,494 - LRK53_RS18170 0.75 -1.4 9,539 - LRK53_RS18170 0.78 -1.9 9,621 + LRK53_RS18170 0.84 -1.9 9,671 - LRK53_RS18170 0.88 +0.9 9,833 + -1.0 9,833 + -0.3 9,833 + -0.7 9,833 + -1.6 9,833 + +0.1 9,833 + -0.3 9,833 + +0.3 9,833 + -2.1 9,833 + -0.4 9,833 + +0.2 9,833 + -1.2 9,833 + -2.8 9,833 + -1.5 9,833 + -0.1 9,833 + -0.1 9,833 + -1.0 9,834 - -0.1 9,834 - -0.3 9,834 - -0.7 9,834 - -0.5 9,834 - -0.8 9,834 - -0.7 9,834 - -0.5 9,834 - -2.5 9,834 - -1.7 9,834 - +0.7 9,834 - +0.3 9,834 - -2.5 9,834 - -0.7 9,834 - -0.8 9,834 - -0.9 9,834 - +0.9 9,834 - -1.2 10,161 - -1.5 10,449 + LRK53_RS18175 0.17 -1.2 10,449 + LRK53_RS18175 0.17 -0.9 10,449 + LRK53_RS18175 0.17 +0.9 10,449 + LRK53_RS18175 0.17 -1.1 10,449 + LRK53_RS18175 0.17 -1.1 10,449 + LRK53_RS18175 0.17 -1.7 10,449 + LRK53_RS18175 0.17 -2.2 10,449 + LRK53_RS18175 0.17 -3.0 10,449 + LRK53_RS18175 0.17 -0.2 10,450 - LRK53_RS18175 0.17 -0.1 10,450 - LRK53_RS18175 0.17 -0.8 10,450 - LRK53_RS18175 0.17 +0.3 10,450 - LRK53_RS18175 0.17 -1.2 10,450 - LRK53_RS18175 0.17 -1.1 10,450 - LRK53_RS18175 0.17 -0.1 10,856 + LRK53_RS18175 0.30 -1.1 10,857 - LRK53_RS18175 0.30 +0.1 10,977 + LRK53_RS18175 0.34 -0.5 10,978 - LRK53_RS18175 0.34 +0.9 10,978 - LRK53_RS18175 0.34 -3.3 11,271 + LRK53_RS18175 0.43 -0.4 11,271 + LRK53_RS18175 0.43 +0.6 11,271 + LRK53_RS18175 0.43 +0.1 11,271 + LRK53_RS18175 0.43 +0.9 11,271 + LRK53_RS18175 0.43 -0.9 11,271 + LRK53_RS18175 0.43 -1.9 11,271 + LRK53_RS18175 0.43 -0.3 11,271 + LRK53_RS18175 0.43 +1.9 11,272 - LRK53_RS18175 0.43 +0.9 11,272 - LRK53_RS18175 0.43 -2.7 11,272 - LRK53_RS18175 0.43 -2.9 11,272 - LRK53_RS18175 0.43 +0.9 11,272 - LRK53_RS18175 0.43 +0.2 11,272 - LRK53_RS18175 0.43 +0.3 11,475 + LRK53_RS18175 0.49 -0.5 11,475 + LRK53_RS18175 0.49 -1.8 11,475 + LRK53_RS18175 0.49 -1.0 11,476 - LRK53_RS18175 0.50 -1.9 11,476 - LRK53_RS18175 0.50 -0.4 11,476 - LRK53_RS18175 0.50 -0.7 11,476 - LRK53_RS18175 0.50 -0.4 11,629 + LRK53_RS18175 0.54 +0.9 11,629 + LRK53_RS18175 0.54 -1.4 11,630 - LRK53_RS18175 0.54 -1.7 11,630 - LRK53_RS18175 0.54 -0.7 11,670 + LRK53_RS18175 0.56 -0.5 11,670 + LRK53_RS18175 0.56 +1.1 11,670 + LRK53_RS18175 0.56 -1.1 11,671 - LRK53_RS18175 0.56 -0.9 11,671 - LRK53_RS18175 0.56 -0.7 11,707 + LRK53_RS18175 0.57 -1.4 11,707 + LRK53_RS18175 0.57 -1.9 11,708 - LRK53_RS18175 0.57 +0.0 11,708 - LRK53_RS18175 0.57 -0.2 11,708 - LRK53_RS18175 0.57 -0.1 11,730 + LRK53_RS18175 0.58 +1.3 11,778 + LRK53_RS18175 0.59 -1.4 11,778 + LRK53_RS18175 0.59 -0.8 11,871 + LRK53_RS18175 0.62 -0.9 11,871 + LRK53_RS18175 0.62 -1.4 11,871 + LRK53_RS18175 0.62 -0.1 11,871 + LRK53_RS18175 0.62 -0.4 11,871 + LRK53_RS18175 0.62 -0.2 11,872 - LRK53_RS18175 0.62 -0.1 11,872 - LRK53_RS18175 0.62 -2.0 11,872 - LRK53_RS18175 0.62 -1.3 11,872 - LRK53_RS18175 0.62 +1.1 11,872 - LRK53_RS18175 0.62 -0.7 11,988 + LRK53_RS18175 0.66 -0.4 12,006 + LRK53_RS18175 0.66 -1.1 12,007 - LRK53_RS18175 0.66 +0.9 12,007 - LRK53_RS18175 0.66 +1.3 12,007 - LRK53_RS18175 0.66 -0.1 12,205 + LRK53_RS18175 0.73 -1.1 12,205 + LRK53_RS18175 0.73 -1.7 12,205 + LRK53_RS18175 0.73 -0.4 12,231 + LRK53_RS18175 0.74 -1.2 12,231 + LRK53_RS18175 0.74 -0.0 12,231 + LRK53_RS18175 0.74 -1.5 12,231 + LRK53_RS18175 0.74 -0.7 12,231 + LRK53_RS18175 0.74 -0.5 12,232 - LRK53_RS18175 0.74 -0.1 12,232 - LRK53_RS18175 0.74 -1.2 12,232 - LRK53_RS18175 0.74 -1.9 12,232 - LRK53_RS18175 0.74 -1.3 12,232 - LRK53_RS18175 0.74 -3.5 12,298 - LRK53_RS18175 0.76 -0.1 12,430 - LRK53_RS18175 0.80 -1.1 12,462 + LRK53_RS18175 0.81 -1.4 12,462 + LRK53_RS18175 0.81 -0.1 12,462 + LRK53_RS18175 0.81 -1.7 12,463 - LRK53_RS18175 0.81 -0.9 12,463 - LRK53_RS18175 0.81 +0.4 12,463 - LRK53_RS18175 0.81 -1.7 12,463 - LRK53_RS18175 0.81 -1.5 12,489 + LRK53_RS18175 0.82 +0.7 12,489 + LRK53_RS18175 0.82 -2.3 12,543 + LRK53_RS18175 0.83 -0.1 12,543 + LRK53_RS18175 0.83 -0.7 12,543 + LRK53_RS18175 0.83 -0.4 12,543 + LRK53_RS18175 0.83 -1.1 12,543 + LRK53_RS18175 0.83 -0.7 12,544 - LRK53_RS18175 0.84 -1.2 12,544 - LRK53_RS18175 0.84 +0.7 12,873 + +0.3 12,873 + -0.1 12,873 + -1.2 12,873 + -0.7 12,873 + +0.9 12,873 + -1.5 12,873 + -0.1 12,873 + -1.9 12,873 + -0.4 12,873 + -0.8 12,873 + -0.7 12,873 + +0.7 12,873 + -0.8 12,874 - -1.9 12,874 - -2.1 12,874 - +0.3 12,874 - -1.8 12,874 - -0.7 12,874 - -2.4 12,874 - -1.9 12,874 - +0.9 12,874 - -0.6 12,874 - +0.3 12,874 - -1.0 12,874 - -1.2 13,180 + -1.1 13,180 + +0.3 13,180 + -2.9 13,180 + -0.2 13,180 + -0.2 13,180 + -0.7 13,180 + -0.1 13,180 + -0.9 13,180 + -1.0 13,181 - -1.4 13,181 - -0.8 13,181 - -0.4 13,181 - +0.1 13,181 - +1.3 13,181 - -1.6 13,181 - -0.3 13,219 + -0.1 13,220 - -1.9 13,220 - -0.3 13,220 - -1.7 13,262 + +0.9 13,262 + -0.3 13,263 - -1.1 13,263 - -1.1 13,263 - -1.2 13,263 - +0.2 13,263 - -2.2 13,263 - -1.2 13,277 - -0.9 13,386 + -0.1 13,386 + -1.2 13,386 + -0.7 13,387 - -0.8 13,387 - -0.2 13,387 - -2.4 13,387 - +0.9 13,410 + -1.3 13,449 + -1.9 13,449 + -1.7 13,449 + +0.1 13,449 + -0.1 13,449 + -1.7 13,449 + -1.8 13,450 - -1.4 13,450 - -2.1 13,450 - -2.9 13,450 - -0.1 13,450 - -1.5 13,468 - LRK53_RS18180 0.10 -0.1 13,516 + LRK53_RS18180 0.15 -1.7 13,516 + LRK53_RS18180 0.15 +0.2 13,517 - LRK53_RS18180 0.15 +0.9 13,578 + LRK53_RS18180 0.20 -0.8 13,578 + LRK53_RS18180 0.20 -1.1 13,579 - LRK53_RS18180 0.20 -1.5 13,579 - LRK53_RS18180 0.20 -1.1 13,926 + LRK53_RS18180 0.51 -0.9 13,927 - LRK53_RS18180 0.51 +0.3 13,927 - LRK53_RS18180 0.51 +0.9 13,927 - LRK53_RS18180 0.51 -0.1 14,033 - LRK53_RS18180 0.61 -0.2 14,049 + LRK53_RS18180 0.62 -0.5 14,049 + LRK53_RS18180 0.62 -0.5 14,050 - LRK53_RS18180 0.62 -1.7 14,050 - LRK53_RS18180 0.62 -1.3
Or see this region's nucleotide sequence