Experiment: R2A_PIPES with Nickel (II) chloride 848 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS13185 and LRK53_RS13190 are separated by 109 nucleotides LRK53_RS13190 and LRK53_RS13195 are separated by 43 nucleotides
LRK53_RS13185: LRK53_RS13185 - NAD(P)H-dependent oxidoreductase, at 2,904,947 to 2,905,516
_RS13185
LRK53_RS13190: LRK53_RS13190 - SAM-dependent methyltransferase, at 2,905,626 to 2,906,525
_RS13190
LRK53_RS13195: LRK53_RS13195 - M61 family metallopeptidase, at 2,906,569 to 2,908,530
_RS13195
Position (kb)
2905
2906
2907 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2904.696 kb on - strand at 2904.749 kb on + strand at 2904.750 kb on - strand at 2904.750 kb on - strand at 2905.205 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.383 kb on + strand, within LRK53_RS13185 at 2905.383 kb on + strand, within LRK53_RS13185 at 2905.383 kb on + strand, within LRK53_RS13185 at 2905.384 kb on - strand, within LRK53_RS13185 at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.541 kb on - strand at 2905.541 kb on - strand at 2905.541 kb on - strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.622 kb on + strand at 2905.623 kb on - strand at 2905.692 kb on + strand at 2905.692 kb on + strand at 2905.693 kb on - strand at 2905.693 kb on - strand at 2905.885 kb on - strand, within LRK53_RS13190 at 2905.885 kb on - strand, within LRK53_RS13190 at 2905.921 kb on - strand, within LRK53_RS13190 at 2906.079 kb on - strand, within LRK53_RS13190 at 2906.079 kb on - strand, within LRK53_RS13190 at 2906.097 kb on + strand, within LRK53_RS13190 at 2906.097 kb on + strand, within LRK53_RS13190 at 2906.097 kb on + strand, within LRK53_RS13190 at 2906.097 kb on + strand, within LRK53_RS13190 at 2906.337 kb on + strand, within LRK53_RS13190 at 2906.352 kb on + strand, within LRK53_RS13190 at 2906.352 kb on + strand, within LRK53_RS13190 at 2906.353 kb on - strand, within LRK53_RS13190 at 2906.400 kb on + strand, within LRK53_RS13190 at 2906.400 kb on + strand, within LRK53_RS13190 at 2906.400 kb on + strand, within LRK53_RS13190 at 2906.400 kb on + strand, within LRK53_RS13190 at 2906.401 kb on - strand, within LRK53_RS13190 at 2906.401 kb on - strand, within LRK53_RS13190 at 2906.401 kb on - strand, within LRK53_RS13190 at 2906.570 kb on + strand at 2906.570 kb on + strand at 2906.570 kb on + strand at 2906.570 kb on + strand at 2906.571 kb on - strand at 2906.571 kb on - strand at 2906.571 kb on - strand at 2906.600 kb on + strand at 2906.600 kb on + strand at 2906.600 kb on + strand at 2906.600 kb on + strand at 2906.600 kb on + strand at 2906.601 kb on - strand at 2906.601 kb on - strand at 2906.601 kb on - strand at 2906.601 kb on - strand at 2906.601 kb on - strand at 2906.637 kb on - strand at 2906.637 kb on - strand at 2906.654 kb on + strand at 2906.654 kb on + strand at 2906.655 kb on - strand at 2906.675 kb on + strand at 2906.676 kb on - strand at 2906.676 kb on - strand at 2906.676 kb on - strand at 2906.687 kb on + strand at 2906.687 kb on + strand at 2906.687 kb on + strand at 2906.687 kb on + strand at 2906.687 kb on + strand at 2906.688 kb on - strand at 2906.688 kb on - strand at 2906.688 kb on - strand at 2906.688 kb on - strand at 2906.760 kb on + strand at 2906.760 kb on + strand at 2906.760 kb on + strand at 2906.761 kb on - strand at 2906.761 kb on - strand at 2906.761 kb on - strand at 2906.762 kb on + strand at 2906.762 kb on + strand at 2906.763 kb on - strand at 2906.770 kb on - strand, within LRK53_RS13195 at 2906.927 kb on + strand, within LRK53_RS13195 at 2906.927 kb on + strand, within LRK53_RS13195 at 2906.939 kb on + strand, within LRK53_RS13195 at 2906.959 kb on - strand, within LRK53_RS13195 at 2906.960 kb on + strand, within LRK53_RS13195 at 2906.960 kb on + strand, within LRK53_RS13195 at 2906.960 kb on + strand, within LRK53_RS13195 at 2906.960 kb on + strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2906.961 kb on - strand, within LRK53_RS13195 at 2907.051 kb on - strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.093 kb on + strand, within LRK53_RS13195 at 2907.094 kb on - strand, within LRK53_RS13195 at 2907.094 kb on - strand, within LRK53_RS13195 at 2907.094 kb on - strand, within LRK53_RS13195 at 2907.094 kb on - strand, within LRK53_RS13195 at 2907.094 kb on - strand, within LRK53_RS13195 at 2907.094 kb on - strand, within LRK53_RS13195 at 2907.094 kb on - strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.095 kb on + strand, within LRK53_RS13195 at 2907.096 kb on - strand, within LRK53_RS13195 at 2907.096 kb on - strand, within LRK53_RS13195 at 2907.096 kb on - strand, within LRK53_RS13195 at 2907.096 kb on - strand, within LRK53_RS13195 at 2907.096 kb on - strand, within LRK53_RS13195 at 2907.096 kb on - strand, within LRK53_RS13195 at 2907.096 kb on - strand, within LRK53_RS13195 at 2907.227 kb on + strand, within LRK53_RS13195 at 2907.227 kb on + strand, within LRK53_RS13195 at 2907.227 kb on + strand, within LRK53_RS13195 at 2907.227 kb on + strand, within LRK53_RS13195 at 2907.227 kb on + strand, within LRK53_RS13195 at 2907.227 kb on + strand, within LRK53_RS13195 at 2907.227 kb on + strand, within LRK53_RS13195 at 2907.228 kb on - strand, within LRK53_RS13195 at 2907.228 kb on - strand, within LRK53_RS13195 at 2907.228 kb on - strand, within LRK53_RS13195 at 2907.228 kb on - strand, within LRK53_RS13195 at 2907.228 kb on - strand, within LRK53_RS13195 at 2907.228 kb on - strand, within LRK53_RS13195 at 2907.228 kb on - strand, within LRK53_RS13195 at 2907.230 kb on + strand, within LRK53_RS13195 at 2907.230 kb on + strand, within LRK53_RS13195 at 2907.230 kb on + strand, within LRK53_RS13195 at 2907.230 kb on + strand, within LRK53_RS13195 at 2907.230 kb on + strand, within LRK53_RS13195 at 2907.231 kb on - strand, within LRK53_RS13195 at 2907.231 kb on - strand, within LRK53_RS13195 at 2907.231 kb on - strand, within LRK53_RS13195 at 2907.231 kb on - strand, within LRK53_RS13195 at 2907.231 kb on - strand, within LRK53_RS13195 at 2907.258 kb on - strand, within LRK53_RS13195 at 2907.312 kb on + strand, within LRK53_RS13195 at 2907.312 kb on + strand, within LRK53_RS13195 at 2907.312 kb on + strand, within LRK53_RS13195 at 2907.312 kb on + strand, within LRK53_RS13195 at 2907.344 kb on + strand, within LRK53_RS13195 at 2907.345 kb on - strand, within LRK53_RS13195 at 2907.345 kb on - strand, within LRK53_RS13195 at 2907.345 kb on - strand, within LRK53_RS13195 at 2907.429 kb on - strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.446 kb on + strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195 at 2907.447 kb on - strand, within LRK53_RS13195
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 848 uM remove 2,904,696 - -2.7 2,904,749 + -1.8 2,904,750 - +0.9 2,904,750 - +0.3 2,905,205 + LRK53_RS13185 0.45 -0.9 2,905,281 + LRK53_RS13185 0.59 -1.3 2,905,281 + LRK53_RS13185 0.59 +1.7 2,905,281 + LRK53_RS13185 0.59 -1.1 2,905,281 + LRK53_RS13185 0.59 -0.1 2,905,282 - LRK53_RS13185 0.59 -0.4 2,905,282 - LRK53_RS13185 0.59 -0.4 2,905,282 - LRK53_RS13185 0.59 +0.6 2,905,282 - LRK53_RS13185 0.59 -1.1 2,905,282 - LRK53_RS13185 0.59 -1.7 2,905,282 - LRK53_RS13185 0.59 -0.1 2,905,282 - LRK53_RS13185 0.59 -0.9 2,905,282 - LRK53_RS13185 0.59 -1.7 2,905,282 - LRK53_RS13185 0.59 -0.7 2,905,282 - LRK53_RS13185 0.59 -0.7 2,905,383 + LRK53_RS13185 0.76 -1.9 2,905,383 + LRK53_RS13185 0.76 -0.7 2,905,383 + LRK53_RS13185 0.76 +0.9 2,905,384 - LRK53_RS13185 0.77 -1.5 2,905,540 + -1.4 2,905,540 + -2.0 2,905,540 + -0.1 2,905,540 + -0.3 2,905,540 + -0.1 2,905,540 + -1.4 2,905,541 - -0.9 2,905,541 - -0.3 2,905,541 - -0.9 2,905,552 + -1.1 2,905,552 + -0.3 2,905,552 + +1.5 2,905,552 + +0.9 2,905,552 + -0.5 2,905,552 + -0.4 2,905,552 + -1.1 2,905,553 - -0.4 2,905,553 - -0.8 2,905,553 - -1.9 2,905,553 - +0.3 2,905,553 - -1.7 2,905,553 - -0.1 2,905,553 - -1.1 2,905,553 - -1.6 2,905,622 + -3.0 2,905,623 - +0.2 2,905,692 + -1.1 2,905,692 + -0.1 2,905,693 - -1.4 2,905,693 - -0.3 2,905,885 - LRK53_RS13190 0.29 -0.4 2,905,885 - LRK53_RS13190 0.29 +0.9 2,905,921 - LRK53_RS13190 0.33 -0.7 2,906,079 - LRK53_RS13190 0.50 -0.6 2,906,079 - LRK53_RS13190 0.50 -1.3 2,906,097 + LRK53_RS13190 0.52 +2.9 2,906,097 + LRK53_RS13190 0.52 -1.9 2,906,097 + LRK53_RS13190 0.52 -2.1 2,906,097 + LRK53_RS13190 0.52 -0.1 2,906,337 + LRK53_RS13190 0.79 -1.1 2,906,352 + LRK53_RS13190 0.81 -1.7 2,906,352 + LRK53_RS13190 0.81 -0.9 2,906,353 - LRK53_RS13190 0.81 -0.9 2,906,400 + LRK53_RS13190 0.86 -0.7 2,906,400 + LRK53_RS13190 0.86 -1.7 2,906,400 + LRK53_RS13190 0.86 -1.4 2,906,400 + LRK53_RS13190 0.86 -1.4 2,906,401 - LRK53_RS13190 0.86 -1.6 2,906,401 - LRK53_RS13190 0.86 -3.0 2,906,401 - LRK53_RS13190 0.86 -1.9 2,906,570 + +0.1 2,906,570 + -1.1 2,906,570 + -1.4 2,906,570 + +0.9 2,906,571 - -0.1 2,906,571 - -0.1 2,906,571 - -0.7 2,906,600 + -0.6 2,906,600 + +1.3 2,906,600 + +1.9 2,906,600 + +0.9 2,906,600 + -2.2 2,906,601 - +0.9 2,906,601 - -0.5 2,906,601 - +1.9 2,906,601 - -0.1 2,906,601 - +0.9 2,906,637 - -0.5 2,906,637 - -0.4 2,906,654 + -0.4 2,906,654 + -1.1 2,906,655 - -0.1 2,906,675 + -1.4 2,906,676 - +1.3 2,906,676 - -0.4 2,906,676 - +1.9 2,906,687 + +0.2 2,906,687 + -1.4 2,906,687 + -0.9 2,906,687 + -1.1 2,906,687 + -1.4 2,906,688 - -1.5 2,906,688 - -0.1 2,906,688 - +0.9 2,906,688 - -0.7 2,906,760 + -0.1 2,906,760 + -1.3 2,906,760 + -0.1 2,906,761 - +0.9 2,906,761 - -0.7 2,906,761 - -0.9 2,906,762 + +0.3 2,906,762 + -0.9 2,906,763 - -0.5 2,906,770 - LRK53_RS13195 0.10 -0.2 2,906,927 + LRK53_RS13195 0.18 -1.3 2,906,927 + LRK53_RS13195 0.18 -0.1 2,906,939 + LRK53_RS13195 0.19 -1.1 2,906,959 - LRK53_RS13195 0.20 -1.1 2,906,960 + LRK53_RS13195 0.20 -0.1 2,906,960 + LRK53_RS13195 0.20 -1.5 2,906,960 + LRK53_RS13195 0.20 +0.3 2,906,960 + LRK53_RS13195 0.20 -0.2 2,906,961 - LRK53_RS13195 0.20 -1.7 2,906,961 - LRK53_RS13195 0.20 -0.4 2,906,961 - LRK53_RS13195 0.20 -0.2 2,906,961 - LRK53_RS13195 0.20 -0.1 2,906,961 - LRK53_RS13195 0.20 -1.4 2,906,961 - LRK53_RS13195 0.20 -1.4 2,906,961 - LRK53_RS13195 0.20 -2.1 2,906,961 - LRK53_RS13195 0.20 +1.5 2,906,961 - LRK53_RS13195 0.20 -0.7 2,906,961 - LRK53_RS13195 0.20 -0.1 2,906,961 - LRK53_RS13195 0.20 -1.4 2,906,961 - LRK53_RS13195 0.20 -0.7 2,907,051 - LRK53_RS13195 0.25 -2.7 2,907,093 + LRK53_RS13195 0.27 +1.5 2,907,093 + LRK53_RS13195 0.27 -1.4 2,907,093 + LRK53_RS13195 0.27 -0.8 2,907,093 + LRK53_RS13195 0.27 -0.1 2,907,093 + LRK53_RS13195 0.27 +0.2 2,907,093 + LRK53_RS13195 0.27 +0.3 2,907,093 + LRK53_RS13195 0.27 +0.2 2,907,093 + LRK53_RS13195 0.27 -0.1 2,907,094 - LRK53_RS13195 0.27 +0.9 2,907,094 - LRK53_RS13195 0.27 -1.4 2,907,094 - LRK53_RS13195 0.27 -0.3 2,907,094 - LRK53_RS13195 0.27 +0.3 2,907,094 - LRK53_RS13195 0.27 -2.5 2,907,094 - LRK53_RS13195 0.27 -1.1 2,907,094 - LRK53_RS13195 0.27 -1.1 2,907,095 + LRK53_RS13195 0.27 -0.8 2,907,095 + LRK53_RS13195 0.27 -0.1 2,907,095 + LRK53_RS13195 0.27 -0.1 2,907,095 + LRK53_RS13195 0.27 -0.5 2,907,095 + LRK53_RS13195 0.27 -1.5 2,907,095 + LRK53_RS13195 0.27 -0.7 2,907,095 + LRK53_RS13195 0.27 -1.8 2,907,095 + LRK53_RS13195 0.27 -1.1 2,907,095 + LRK53_RS13195 0.27 -1.1 2,907,095 + LRK53_RS13195 0.27 -0.9 2,907,095 + LRK53_RS13195 0.27 +0.9 2,907,095 + LRK53_RS13195 0.27 -1.4 2,907,095 + LRK53_RS13195 0.27 -0.7 2,907,095 + LRK53_RS13195 0.27 -0.9 2,907,095 + LRK53_RS13195 0.27 -0.3 2,907,096 - LRK53_RS13195 0.27 +0.9 2,907,096 - LRK53_RS13195 0.27 -1.5 2,907,096 - LRK53_RS13195 0.27 -0.5 2,907,096 - LRK53_RS13195 0.27 -0.7 2,907,096 - LRK53_RS13195 0.27 +0.9 2,907,096 - LRK53_RS13195 0.27 +0.9 2,907,096 - LRK53_RS13195 0.27 -0.8 2,907,227 + LRK53_RS13195 0.34 -1.7 2,907,227 + LRK53_RS13195 0.34 -0.4 2,907,227 + LRK53_RS13195 0.34 +2.5 2,907,227 + LRK53_RS13195 0.34 -0.7 2,907,227 + LRK53_RS13195 0.34 -1.3 2,907,227 + LRK53_RS13195 0.34 -1.1 2,907,227 + LRK53_RS13195 0.34 -0.1 2,907,228 - LRK53_RS13195 0.34 -0.9 2,907,228 - LRK53_RS13195 0.34 -1.1 2,907,228 - LRK53_RS13195 0.34 -1.1 2,907,228 - LRK53_RS13195 0.34 +0.5 2,907,228 - LRK53_RS13195 0.34 -0.7 2,907,228 - LRK53_RS13195 0.34 -0.1 2,907,228 - LRK53_RS13195 0.34 -0.3 2,907,230 + LRK53_RS13195 0.34 -0.5 2,907,230 + LRK53_RS13195 0.34 +0.9 2,907,230 + LRK53_RS13195 0.34 +0.3 2,907,230 + LRK53_RS13195 0.34 +0.9 2,907,230 + LRK53_RS13195 0.34 -1.3 2,907,231 - LRK53_RS13195 0.34 +0.9 2,907,231 - LRK53_RS13195 0.34 -0.6 2,907,231 - LRK53_RS13195 0.34 -1.1 2,907,231 - LRK53_RS13195 0.34 +0.9 2,907,231 - LRK53_RS13195 0.34 -0.6 2,907,258 - LRK53_RS13195 0.35 -0.7 2,907,312 + LRK53_RS13195 0.38 -0.2 2,907,312 + LRK53_RS13195 0.38 -2.1 2,907,312 + LRK53_RS13195 0.38 +0.9 2,907,312 + LRK53_RS13195 0.38 -0.7 2,907,344 + LRK53_RS13195 0.40 -0.1 2,907,345 - LRK53_RS13195 0.40 -1.1 2,907,345 - LRK53_RS13195 0.40 +0.9 2,907,345 - LRK53_RS13195 0.40 +0.5 2,907,429 - LRK53_RS13195 0.44 -0.4 2,907,446 + LRK53_RS13195 0.45 -0.7 2,907,446 + LRK53_RS13195 0.45 +0.1 2,907,446 + LRK53_RS13195 0.45 -0.7 2,907,446 + LRK53_RS13195 0.45 -1.1 2,907,446 + LRK53_RS13195 0.45 -0.1 2,907,446 + LRK53_RS13195 0.45 -2.1 2,907,446 + LRK53_RS13195 0.45 -0.3 2,907,446 + LRK53_RS13195 0.45 -1.3 2,907,446 + LRK53_RS13195 0.45 -1.9 2,907,446 + LRK53_RS13195 0.45 -0.7 2,907,447 - LRK53_RS13195 0.45 -1.4 2,907,447 - LRK53_RS13195 0.45 -1.8 2,907,447 - LRK53_RS13195 0.45 -1.1 2,907,447 - LRK53_RS13195 0.45 +0.3 2,907,447 - LRK53_RS13195 0.45 -0.2 2,907,447 - LRK53_RS13195 0.45 +0.3 2,907,447 - LRK53_RS13195 0.45 +1.5 2,907,447 - LRK53_RS13195 0.45 +0.9
Or see this region's nucleotide sequence