Experiment: R2A_PIPES with Nickel (II) chloride 848 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08765 and LRK53_RS08770 are separated by 115 nucleotides LRK53_RS08770 and LRK53_RS08775 are separated by 134 nucleotides LRK53_RS08775 and LRK53_RS08780 are separated by 28 nucleotides LRK53_RS08780 and LRK53_RS08785 are separated by 105 nucleotides
LRK53_RS08765: LRK53_RS08765 - histidine--tRNA ligase, at 1,972,150 to 1,973,523
_RS08765
LRK53_RS08770: LRK53_RS08770 - GIY-YIG nuclease family protein, at 1,973,639 to 1,973,932
_RS08770
LRK53_RS08775: LRK53_RS08775 - aromatic amino acid ammonia-lyase, at 1,974,067 to 1,975,956
_RS08775
LRK53_RS08780: LRK53_RS08780 - DUF6165 family protein, at 1,975,985 to 1,976,383
_RS08780
LRK53_RS08785: LRK53_RS08785 - glycosyltransferase family 9 protein, at 1,976,489 to 1,977,532
_RS08785
Position (kb) 1974
1975
1976 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.765 kb on - strand, within LRK53_RS08770 at 1973.774 kb on + strand, within LRK53_RS08770 at 1973.930 kb on + strand at 1973.931 kb on - strand at 1973.931 kb on - strand at 1974.064 kb on + strand at 1974.064 kb on + strand at 1974.065 kb on - strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.230 kb on - strand at 1974.230 kb on - strand at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.372 kb on - strand, within LRK53_RS08775 at 1974.372 kb on - strand, within LRK53_RS08775 at 1974.468 kb on + strand, within LRK53_RS08775 at 1974.469 kb on - strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.608 kb on - strand, within LRK53_RS08775 at 1974.608 kb on - strand, within LRK53_RS08775 at 1974.608 kb on - strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.925 kb on + strand, within LRK53_RS08775 at 1974.925 kb on + strand, within LRK53_RS08775 at 1974.926 kb on - strand, within LRK53_RS08775 at 1974.926 kb on - strand, within LRK53_RS08775 at 1975.054 kb on + strand, within LRK53_RS08775 at 1975.054 kb on + strand, within LRK53_RS08775 at 1975.055 kb on - strand, within LRK53_RS08775 at 1975.055 kb on - strand, within LRK53_RS08775 at 1975.055 kb on - strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.109 kb on - strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.453 kb on + strand, within LRK53_RS08775 at 1975.453 kb on + strand, within LRK53_RS08775 at 1975.454 kb on - strand, within LRK53_RS08775 at 1975.510 kb on + strand, within LRK53_RS08775 at 1975.510 kb on + strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.626 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.754 kb on - strand, within LRK53_RS08775 at 1975.754 kb on - strand, within LRK53_RS08775 at 1975.754 kb on - strand, within LRK53_RS08775 at 1976.395 kb on - strand at 1976.490 kb on + strand at 1976.490 kb on + strand at 1976.490 kb on + strand at 1976.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 848 uM remove 1,973,534 + -0.7 1,973,534 + -1.1 1,973,534 + +2.3 1,973,534 + -1.7 1,973,534 + -1.0 1,973,535 - +0.9 1,973,535 - -2.1 1,973,535 - -1.7 1,973,535 - -1.1 1,973,535 - -2.8 1,973,663 + -1.1 1,973,663 + +0.4 1,973,663 + +0.3 1,973,663 + -0.5 1,973,663 + -1.7 1,973,664 - -1.4 1,973,664 - +3.2 1,973,664 - -1.0 1,973,664 - -1.1 1,973,664 - -0.1 1,973,696 + LRK53_RS08770 0.19 +0.9 1,973,696 + LRK53_RS08770 0.19 -0.4 1,973,696 + LRK53_RS08770 0.19 +0.6 1,973,696 + LRK53_RS08770 0.19 -2.0 1,973,696 + LRK53_RS08770 0.19 +0.1 1,973,696 + LRK53_RS08770 0.19 +1.3 1,973,696 + LRK53_RS08770 0.19 -0.4 1,973,696 + LRK53_RS08770 0.19 -1.9 1,973,696 + LRK53_RS08770 0.19 -1.4 1,973,696 + LRK53_RS08770 0.19 -1.3 1,973,696 + LRK53_RS08770 0.19 -1.1 1,973,696 + LRK53_RS08770 0.19 -0.8 1,973,696 + LRK53_RS08770 0.19 +0.9 1,973,696 + LRK53_RS08770 0.19 -1.1 1,973,696 + LRK53_RS08770 0.19 +0.4 1,973,697 - LRK53_RS08770 0.20 -1.7 1,973,697 - LRK53_RS08770 0.20 -0.7 1,973,697 - LRK53_RS08770 0.20 +0.1 1,973,697 - LRK53_RS08770 0.20 -0.7 1,973,697 - LRK53_RS08770 0.20 -0.7 1,973,697 - LRK53_RS08770 0.20 -1.4 1,973,697 - LRK53_RS08770 0.20 -1.7 1,973,697 - LRK53_RS08770 0.20 -0.1 1,973,697 - LRK53_RS08770 0.20 +0.9 1,973,697 - LRK53_RS08770 0.20 -1.7 1,973,697 - LRK53_RS08770 0.20 +1.5 1,973,697 - LRK53_RS08770 0.20 -1.1 1,973,764 + LRK53_RS08770 0.43 -0.7 1,973,764 + LRK53_RS08770 0.43 +0.6 1,973,764 + LRK53_RS08770 0.43 +0.9 1,973,764 + LRK53_RS08770 0.43 -1.2 1,973,764 + LRK53_RS08770 0.43 -0.3 1,973,764 + LRK53_RS08770 0.43 +0.3 1,973,765 - LRK53_RS08770 0.43 -1.1 1,973,774 + LRK53_RS08770 0.46 +0.1 1,973,930 + +0.4 1,973,931 - -0.4 1,973,931 - -0.3 1,974,064 + +0.9 1,974,064 + -0.9 1,974,065 - -1.4 1,974,229 + -1.1 1,974,229 + -0.1 1,974,229 + -1.1 1,974,229 + -0.7 1,974,229 + -1.8 1,974,229 + -2.1 1,974,230 - +2.5 1,974,230 - -0.7 1,974,371 + LRK53_RS08775 0.16 -1.1 1,974,371 + LRK53_RS08775 0.16 +0.9 1,974,371 + LRK53_RS08775 0.16 -1.1 1,974,371 + LRK53_RS08775 0.16 -0.7 1,974,372 - LRK53_RS08775 0.16 +0.3 1,974,372 - LRK53_RS08775 0.16 -1.7 1,974,468 + LRK53_RS08775 0.21 -0.1 1,974,469 - LRK53_RS08775 0.21 -0.7 1,974,607 + LRK53_RS08775 0.29 -1.6 1,974,607 + LRK53_RS08775 0.29 -1.7 1,974,607 + LRK53_RS08775 0.29 -1.1 1,974,607 + LRK53_RS08775 0.29 -1.9 1,974,607 + LRK53_RS08775 0.29 -0.1 1,974,607 + LRK53_RS08775 0.29 -1.1 1,974,607 + LRK53_RS08775 0.29 -0.1 1,974,607 + LRK53_RS08775 0.29 -0.7 1,974,608 - LRK53_RS08775 0.29 -1.1 1,974,608 - LRK53_RS08775 0.29 +0.9 1,974,608 - LRK53_RS08775 0.29 +1.6 1,974,884 + LRK53_RS08775 0.43 +0.3 1,974,884 + LRK53_RS08775 0.43 +0.3 1,974,884 + LRK53_RS08775 0.43 +0.9 1,974,884 + LRK53_RS08775 0.43 +0.9 1,974,884 + LRK53_RS08775 0.43 +0.9 1,974,884 + LRK53_RS08775 0.43 -0.7 1,974,884 + LRK53_RS08775 0.43 -0.9 1,974,884 + LRK53_RS08775 0.43 -0.3 1,974,884 + LRK53_RS08775 0.43 +0.9 1,974,884 + LRK53_RS08775 0.43 +0.9 1,974,884 + LRK53_RS08775 0.43 -1.4 1,974,885 - LRK53_RS08775 0.43 +0.9 1,974,885 - LRK53_RS08775 0.43 -1.9 1,974,885 - LRK53_RS08775 0.43 -0.7 1,974,885 - LRK53_RS08775 0.43 +0.9 1,974,885 - LRK53_RS08775 0.43 -1.1 1,974,885 - LRK53_RS08775 0.43 +0.5 1,974,885 - LRK53_RS08775 0.43 -1.5 1,974,885 - LRK53_RS08775 0.43 -2.1 1,974,885 - LRK53_RS08775 0.43 +0.9 1,974,885 - LRK53_RS08775 0.43 -0.7 1,974,925 + LRK53_RS08775 0.45 -1.4 1,974,925 + LRK53_RS08775 0.45 +1.9 1,974,926 - LRK53_RS08775 0.45 +0.9 1,974,926 - LRK53_RS08775 0.45 -0.7 1,975,054 + LRK53_RS08775 0.52 -0.7 1,975,054 + LRK53_RS08775 0.52 -0.1 1,975,055 - LRK53_RS08775 0.52 +2.5 1,975,055 - LRK53_RS08775 0.52 -0.4 1,975,055 - LRK53_RS08775 0.52 -0.7 1,975,108 + LRK53_RS08775 0.55 -0.1 1,975,108 + LRK53_RS08775 0.55 -1.4 1,975,108 + LRK53_RS08775 0.55 +0.1 1,975,108 + LRK53_RS08775 0.55 -1.9 1,975,108 + LRK53_RS08775 0.55 -1.7 1,975,108 + LRK53_RS08775 0.55 -1.8 1,975,109 - LRK53_RS08775 0.55 +0.9 1,975,309 + LRK53_RS08775 0.66 +0.5 1,975,309 + LRK53_RS08775 0.66 -1.6 1,975,309 + LRK53_RS08775 0.66 +0.9 1,975,309 + LRK53_RS08775 0.66 -3.2 1,975,309 + LRK53_RS08775 0.66 -1.2 1,975,310 - LRK53_RS08775 0.66 -0.7 1,975,310 - LRK53_RS08775 0.66 -1.9 1,975,310 - LRK53_RS08775 0.66 -0.1 1,975,310 - LRK53_RS08775 0.66 -1.8 1,975,310 - LRK53_RS08775 0.66 -0.7 1,975,453 + LRK53_RS08775 0.73 -0.1 1,975,453 + LRK53_RS08775 0.73 -1.1 1,975,454 - LRK53_RS08775 0.73 -0.3 1,975,510 + LRK53_RS08775 0.76 -0.7 1,975,510 + LRK53_RS08775 0.76 -2.3 1,975,511 - LRK53_RS08775 0.76 +0.9 1,975,511 - LRK53_RS08775 0.76 -0.1 1,975,511 - LRK53_RS08775 0.76 -0.4 1,975,511 - LRK53_RS08775 0.76 -2.3 1,975,511 - LRK53_RS08775 0.76 -0.1 1,975,511 - LRK53_RS08775 0.76 -2.1 1,975,624 + LRK53_RS08775 0.82 -0.1 1,975,624 + LRK53_RS08775 0.82 -0.3 1,975,624 + LRK53_RS08775 0.82 -1.8 1,975,624 + LRK53_RS08775 0.82 -1.1 1,975,624 + LRK53_RS08775 0.82 -2.2 1,975,624 + LRK53_RS08775 0.82 -2.1 1,975,624 + LRK53_RS08775 0.82 -0.7 1,975,624 + LRK53_RS08775 0.82 +0.9 1,975,624 + LRK53_RS08775 0.82 +0.9 1,975,624 + LRK53_RS08775 0.82 -1.1 1,975,624 + LRK53_RS08775 0.82 -0.7 1,975,624 + LRK53_RS08775 0.82 -0.1 1,975,625 - LRK53_RS08775 0.82 -0.1 1,975,625 - LRK53_RS08775 0.82 -1.4 1,975,625 - LRK53_RS08775 0.82 -2.3 1,975,625 - LRK53_RS08775 0.82 +0.5 1,975,625 - LRK53_RS08775 0.82 -1.4 1,975,625 - LRK53_RS08775 0.82 -2.1 1,975,625 - LRK53_RS08775 0.82 -0.1 1,975,625 - LRK53_RS08775 0.82 -1.8 1,975,625 - LRK53_RS08775 0.82 -1.7 1,975,625 - LRK53_RS08775 0.82 -0.1 1,975,626 + LRK53_RS08775 0.82 -0.1 1,975,753 + LRK53_RS08775 0.89 -0.1 1,975,753 + LRK53_RS08775 0.89 -1.4 1,975,753 + LRK53_RS08775 0.89 +0.9 1,975,753 + LRK53_RS08775 0.89 -1.7 1,975,754 - LRK53_RS08775 0.89 +0.6 1,975,754 - LRK53_RS08775 0.89 -1.1 1,975,754 - LRK53_RS08775 0.89 +0.9 1,976,395 - -0.7 1,976,490 + -0.1 1,976,490 + -0.1 1,976,490 + +0.9 1,976,490 + -0.7
Or see this region's nucleotide sequence