Experiment: R2A_PIPES with Nickel (II) chloride 848 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05630 and LRK53_RS05635 are separated by 24 nucleotides LRK53_RS05635 and LRK53_RS05640 are separated by 111 nucleotides LRK53_RS05640 and LRK53_RS05645 are separated by 50 nucleotides LRK53_RS05645 and LRK53_RS05650 are separated by 234 nucleotides LRK53_RS05650 and LRK53_RS05655 overlap by 4 nucleotides
LRK53_RS05630: LRK53_RS05630 - SDR family oxidoreductase, at 1,311,223 to 1,312,056
_RS05630
LRK53_RS05635: LRK53_RS05635 - YbjQ family protein, at 1,312,081 to 1,312,425
_RS05635
LRK53_RS05640: LRK53_RS05640 - dodecin domain-containing protein, at 1,312,537 to 1,312,740
_RS05640
LRK53_RS05645: LRK53_RS05645 - kinase, at 1,312,791 to 1,313,621
_RS05645
LRK53_RS05650: LRK53_RS05650 - pyridoxamine 5'-phosphate oxidase, at 1,313,856 to 1,314,443
_RS05650
LRK53_RS05655: LRK53_RS05655 - secondary thiamine-phosphate synthase enzyme YjbQ, at 1,314,440 to 1,314,880
_RS05655
Position (kb)
1312
1313
1314 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1311.850 kb on + strand, within LRK53_RS05630 at 1311.850 kb on + strand, within LRK53_RS05630 at 1311.850 kb on + strand, within LRK53_RS05630 at 1311.850 kb on + strand, within LRK53_RS05630 at 1311.850 kb on + strand, within LRK53_RS05630 at 1311.851 kb on - strand, within LRK53_RS05630 at 1311.851 kb on - strand, within LRK53_RS05630 at 1311.851 kb on - strand, within LRK53_RS05630 at 1311.851 kb on - strand, within LRK53_RS05630 at 1311.851 kb on - strand, within LRK53_RS05630 at 1311.851 kb on - strand, within LRK53_RS05630 at 1311.851 kb on - strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.892 kb on + strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1311.893 kb on - strand, within LRK53_RS05630 at 1312.249 kb on + strand, within LRK53_RS05635 at 1312.249 kb on + strand, within LRK53_RS05635 at 1312.249 kb on + strand, within LRK53_RS05635 at 1312.249 kb on + strand, within LRK53_RS05635 at 1312.250 kb on - strand, within LRK53_RS05635 at 1312.250 kb on - strand, within LRK53_RS05635 at 1312.250 kb on - strand, within LRK53_RS05635 at 1312.250 kb on - strand, within LRK53_RS05635 at 1312.345 kb on + strand, within LRK53_RS05635 at 1312.345 kb on + strand, within LRK53_RS05635 at 1312.345 kb on + strand, within LRK53_RS05635 at 1312.390 kb on + strand, within LRK53_RS05635 at 1312.831 kb on + strand at 1312.832 kb on - strand at 1312.844 kb on + strand at 1313.410 kb on + strand, within LRK53_RS05645 at 1313.410 kb on + strand, within LRK53_RS05645 at 1313.410 kb on + strand, within LRK53_RS05645 at 1313.411 kb on - strand, within LRK53_RS05645 at 1313.411 kb on - strand, within LRK53_RS05645 at 1313.413 kb on + strand, within LRK53_RS05645 at 1313.413 kb on + strand, within LRK53_RS05645 at 1313.413 kb on + strand, within LRK53_RS05645 at 1313.413 kb on + strand, within LRK53_RS05645 at 1313.413 kb on + strand, within LRK53_RS05645 at 1313.413 kb on + strand, within LRK53_RS05645 at 1313.413 kb on + strand, within LRK53_RS05645 at 1313.414 kb on - strand, within LRK53_RS05645 at 1313.414 kb on - strand, within LRK53_RS05645 at 1313.414 kb on - strand, within LRK53_RS05645 at 1313.414 kb on - strand, within LRK53_RS05645 at 1313.414 kb on - strand, within LRK53_RS05645 at 1313.414 kb on - strand, within LRK53_RS05645 at 1313.475 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.530 kb on + strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.531 kb on - strand, within LRK53_RS05645 at 1313.563 kb on + strand at 1313.782 kb on + strand at 1313.782 kb on + strand at 1313.782 kb on + strand at 1313.782 kb on + strand at 1313.782 kb on + strand at 1313.782 kb on + strand at 1313.783 kb on - strand at 1313.783 kb on - strand at 1313.783 kb on - strand at 1313.783 kb on - strand at 1313.783 kb on - strand at 1313.783 kb on - strand at 1313.875 kb on + strand at 1313.875 kb on + strand at 1313.875 kb on + strand at 1313.875 kb on + strand at 1313.876 kb on - strand at 1313.876 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 848 uM remove 1,311,850 + LRK53_RS05630 0.75 -0.1 1,311,850 + LRK53_RS05630 0.75 -0.1 1,311,850 + LRK53_RS05630 0.75 -1.2 1,311,850 + LRK53_RS05630 0.75 -1.4 1,311,850 + LRK53_RS05630 0.75 +0.3 1,311,851 - LRK53_RS05630 0.75 +0.9 1,311,851 - LRK53_RS05630 0.75 -1.9 1,311,851 - LRK53_RS05630 0.75 -0.7 1,311,851 - LRK53_RS05630 0.75 -0.8 1,311,851 - LRK53_RS05630 0.75 -0.1 1,311,851 - LRK53_RS05630 0.75 +2.5 1,311,851 - LRK53_RS05630 0.75 -0.4 1,311,892 + LRK53_RS05630 0.80 -2.1 1,311,892 + LRK53_RS05630 0.80 -2.0 1,311,892 + LRK53_RS05630 0.80 -1.4 1,311,892 + LRK53_RS05630 0.80 -0.9 1,311,892 + LRK53_RS05630 0.80 -1.9 1,311,892 + LRK53_RS05630 0.80 -0.2 1,311,892 + LRK53_RS05630 0.80 +0.9 1,311,892 + LRK53_RS05630 0.80 -2.2 1,311,892 + LRK53_RS05630 0.80 +0.0 1,311,892 + LRK53_RS05630 0.80 -0.4 1,311,893 - LRK53_RS05630 0.80 -0.3 1,311,893 - LRK53_RS05630 0.80 +0.1 1,311,893 - LRK53_RS05630 0.80 -1.4 1,311,893 - LRK53_RS05630 0.80 -0.1 1,311,893 - LRK53_RS05630 0.80 -1.4 1,311,893 - LRK53_RS05630 0.80 -0.5 1,311,893 - LRK53_RS05630 0.80 -1.1 1,311,893 - LRK53_RS05630 0.80 -1.1 1,311,893 - LRK53_RS05630 0.80 -0.1 1,312,249 + LRK53_RS05635 0.49 -1.3 1,312,249 + LRK53_RS05635 0.49 -1.4 1,312,249 + LRK53_RS05635 0.49 -0.1 1,312,249 + LRK53_RS05635 0.49 -1.1 1,312,250 - LRK53_RS05635 0.49 -1.2 1,312,250 - LRK53_RS05635 0.49 -0.7 1,312,250 - LRK53_RS05635 0.49 +0.9 1,312,250 - LRK53_RS05635 0.49 -1.2 1,312,345 + LRK53_RS05635 0.77 -1.5 1,312,345 + LRK53_RS05635 0.77 -2.1 1,312,345 + LRK53_RS05635 0.77 -1.1 1,312,390 + LRK53_RS05635 0.90 -1.1 1,312,831 + -0.1 1,312,832 - -1.7 1,312,844 + -0.7 1,313,410 + LRK53_RS05645 0.74 +0.9 1,313,410 + LRK53_RS05645 0.74 -1.4 1,313,410 + LRK53_RS05645 0.74 -1.1 1,313,411 - LRK53_RS05645 0.75 -1.4 1,313,411 - LRK53_RS05645 0.75 -1.4 1,313,413 + LRK53_RS05645 0.75 +0.9 1,313,413 + LRK53_RS05645 0.75 -0.1 1,313,413 + LRK53_RS05645 0.75 +1.5 1,313,413 + LRK53_RS05645 0.75 -0.1 1,313,413 + LRK53_RS05645 0.75 -0.8 1,313,413 + LRK53_RS05645 0.75 +0.9 1,313,413 + LRK53_RS05645 0.75 -0.7 1,313,414 - LRK53_RS05645 0.75 -0.7 1,313,414 - LRK53_RS05645 0.75 +0.9 1,313,414 - LRK53_RS05645 0.75 -1.9 1,313,414 - LRK53_RS05645 0.75 -0.7 1,313,414 - LRK53_RS05645 0.75 +0.9 1,313,414 - LRK53_RS05645 0.75 +0.9 1,313,475 + LRK53_RS05645 0.82 -0.7 1,313,530 + LRK53_RS05645 0.89 -1.1 1,313,530 + LRK53_RS05645 0.89 -1.7 1,313,530 + LRK53_RS05645 0.89 -2.1 1,313,530 + LRK53_RS05645 0.89 -2.5 1,313,530 + LRK53_RS05645 0.89 +0.1 1,313,530 + LRK53_RS05645 0.89 +0.3 1,313,530 + LRK53_RS05645 0.89 -0.4 1,313,530 + LRK53_RS05645 0.89 +0.9 1,313,531 - LRK53_RS05645 0.89 -2.1 1,313,531 - LRK53_RS05645 0.89 -0.7 1,313,531 - LRK53_RS05645 0.89 +0.3 1,313,531 - LRK53_RS05645 0.89 -1.4 1,313,531 - LRK53_RS05645 0.89 -0.1 1,313,531 - LRK53_RS05645 0.89 +1.5 1,313,531 - LRK53_RS05645 0.89 -2.3 1,313,531 - LRK53_RS05645 0.89 -0.1 1,313,531 - LRK53_RS05645 0.89 -1.9 1,313,531 - LRK53_RS05645 0.89 +0.9 1,313,531 - LRK53_RS05645 0.89 -1.0 1,313,563 + +0.9 1,313,782 + -2.9 1,313,782 + -1.1 1,313,782 + -0.4 1,313,782 + -1.4 1,313,782 + -1.7 1,313,782 + -1.1 1,313,783 - -3.0 1,313,783 - -1.1 1,313,783 - +1.9 1,313,783 - -0.1 1,313,783 - -1.0 1,313,783 - -0.7 1,313,875 + -1.8 1,313,875 + -0.1 1,313,875 + -0.8 1,313,875 + -0.6 1,313,876 - +1.9 1,313,876 - +0.3
Or see this region's nucleotide sequence