Experiment: R2A_PIPES with Nickel (II) chloride 848 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02460 and LRK53_RS02465 are separated by 128 nucleotides LRK53_RS02465 and LRK53_RS02470 are separated by 76 nucleotides LRK53_RS02470 and LRK53_RS02475 are separated by 3 nucleotides
LRK53_RS02460: LRK53_RS02460 - GH1 family beta-glucosidase, at 565,568 to 566,923
_RS02460
LRK53_RS02465: LRK53_RS02465 - sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC, at 567,052 to 568,143
_RS02465
LRK53_RS02470: LRK53_RS02470 - ParA family protein, at 568,220 to 569,092
_RS02470
LRK53_RS02475: LRK53_RS02475 - ParB/RepB/Spo0J family partition protein, at 569,096 to 569,965
_RS02475
Position (kb) 567
568
569 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 566.146 kb on - strand, within LRK53_RS02460 at 566.146 kb on - strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.187 kb on + strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.188 kb on - strand, within LRK53_RS02460 at 566.241 kb on + strand, within LRK53_RS02460 at 566.242 kb on - strand, within LRK53_RS02460 at 566.261 kb on + strand, within LRK53_RS02460 at 566.261 kb on + strand, within LRK53_RS02460 at 566.261 kb on + strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.262 kb on - strand, within LRK53_RS02460 at 566.295 kb on + strand, within LRK53_RS02460 at 566.295 kb on + strand, within LRK53_RS02460 at 566.296 kb on - strand, within LRK53_RS02460 at 566.355 kb on + strand, within LRK53_RS02460 at 566.355 kb on + strand, within LRK53_RS02460 at 566.355 kb on + strand, within LRK53_RS02460 at 566.355 kb on + strand, within LRK53_RS02460 at 566.355 kb on + strand, within LRK53_RS02460 at 566.355 kb on + strand, within LRK53_RS02460 at 566.355 kb on + strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.356 kb on - strand, within LRK53_RS02460 at 566.395 kb on - strand, within LRK53_RS02460 at 566.395 kb on - strand, within LRK53_RS02460 at 566.481 kb on + strand, within LRK53_RS02460 at 566.616 kb on + strand, within LRK53_RS02460 at 566.616 kb on + strand, within LRK53_RS02460 at 566.616 kb on + strand, within LRK53_RS02460 at 566.617 kb on - strand, within LRK53_RS02460 at 566.736 kb on + strand, within LRK53_RS02460 at 566.951 kb on + strand at 566.952 kb on - strand at 566.952 kb on - strand at 566.959 kb on + strand at 566.972 kb on + strand at 566.972 kb on + strand at 566.973 kb on - strand at 566.973 kb on - strand at 566.973 kb on - strand at 566.973 kb on - strand at 566.973 kb on - strand at 566.973 kb on - strand at 566.973 kb on - strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.062 kb on + strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.063 kb on - strand at 567.088 kb on + strand at 567.089 kb on - strand at 567.304 kb on + strand, within LRK53_RS02465 at 567.304 kb on + strand, within LRK53_RS02465 at 567.304 kb on + strand, within LRK53_RS02465 at 567.305 kb on - strand, within LRK53_RS02465 at 567.305 kb on - strand, within LRK53_RS02465 at 567.305 kb on - strand, within LRK53_RS02465 at 567.305 kb on - strand, within LRK53_RS02465 at 567.313 kb on + strand, within LRK53_RS02465 at 567.314 kb on - strand, within LRK53_RS02465 at 567.314 kb on - strand, within LRK53_RS02465 at 567.619 kb on + strand, within LRK53_RS02465 at 567.619 kb on + strand, within LRK53_RS02465 at 567.619 kb on + strand, within LRK53_RS02465 at 567.619 kb on + strand, within LRK53_RS02465 at 567.619 kb on + strand, within LRK53_RS02465 at 567.620 kb on - strand, within LRK53_RS02465 at 567.620 kb on - strand, within LRK53_RS02465 at 567.620 kb on - strand, within LRK53_RS02465 at 567.620 kb on - strand, within LRK53_RS02465 at 567.620 kb on - strand, within LRK53_RS02465 at 567.620 kb on - strand, within LRK53_RS02465 at 567.620 kb on - strand, within LRK53_RS02465 at 567.718 kb on + strand, within LRK53_RS02465 at 567.718 kb on + strand, within LRK53_RS02465 at 567.718 kb on + strand, within LRK53_RS02465 at 567.718 kb on + strand, within LRK53_RS02465 at 567.718 kb on + strand, within LRK53_RS02465 at 567.718 kb on + strand, within LRK53_RS02465 at 567.719 kb on - strand, within LRK53_RS02465 at 567.719 kb on - strand, within LRK53_RS02465 at 567.719 kb on - strand, within LRK53_RS02465 at 568.182 kb on + strand at 568.182 kb on + strand at 568.182 kb on + strand at 568.182 kb on + strand at 568.182 kb on + strand at 568.182 kb on + strand at 568.182 kb on + strand at 568.182 kb on + strand at 568.207 kb on + strand at 568.207 kb on + strand at 568.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 848 uM remove 566,146 - LRK53_RS02460 0.43 -1.6 566,146 - LRK53_RS02460 0.43 +1.0 566,187 + LRK53_RS02460 0.46 +0.7 566,187 + LRK53_RS02460 0.46 -1.3 566,187 + LRK53_RS02460 0.46 +0.0 566,187 + LRK53_RS02460 0.46 +1.0 566,187 + LRK53_RS02460 0.46 -0.8 566,187 + LRK53_RS02460 0.46 +0.0 566,187 + LRK53_RS02460 0.46 -1.6 566,187 + LRK53_RS02460 0.46 +2.0 566,188 - LRK53_RS02460 0.46 -0.3 566,188 - LRK53_RS02460 0.46 +0.4 566,188 - LRK53_RS02460 0.46 -0.6 566,188 - LRK53_RS02460 0.46 -1.4 566,188 - LRK53_RS02460 0.46 -0.6 566,188 - LRK53_RS02460 0.46 -1.8 566,188 - LRK53_RS02460 0.46 -1.6 566,188 - LRK53_RS02460 0.46 -1.2 566,188 - LRK53_RS02460 0.46 +0.3 566,188 - LRK53_RS02460 0.46 +0.0 566,188 - LRK53_RS02460 0.46 -1.3 566,188 - LRK53_RS02460 0.46 +1.0 566,188 - LRK53_RS02460 0.46 -1.0 566,188 - LRK53_RS02460 0.46 -0.2 566,241 + LRK53_RS02460 0.50 -1.3 566,242 - LRK53_RS02460 0.50 -0.8 566,261 + LRK53_RS02460 0.51 -1.6 566,261 + LRK53_RS02460 0.51 +1.6 566,261 + LRK53_RS02460 0.51 -2.2 566,262 - LRK53_RS02460 0.51 -1.0 566,262 - LRK53_RS02460 0.51 -1.3 566,262 - LRK53_RS02460 0.51 -1.8 566,262 - LRK53_RS02460 0.51 +0.3 566,262 - LRK53_RS02460 0.51 -0.6 566,262 - LRK53_RS02460 0.51 -0.8 566,262 - LRK53_RS02460 0.51 +1.0 566,262 - LRK53_RS02460 0.51 -1.6 566,295 + LRK53_RS02460 0.54 -0.6 566,295 + LRK53_RS02460 0.54 +1.0 566,296 - LRK53_RS02460 0.54 +0.0 566,355 + LRK53_RS02460 0.58 -1.0 566,355 + LRK53_RS02460 0.58 -1.3 566,355 + LRK53_RS02460 0.58 -1.3 566,355 + LRK53_RS02460 0.58 -1.6 566,355 + LRK53_RS02460 0.58 -1.0 566,355 + LRK53_RS02460 0.58 -0.6 566,355 + LRK53_RS02460 0.58 -1.1 566,356 - LRK53_RS02460 0.58 -0.6 566,356 - LRK53_RS02460 0.58 +2.0 566,356 - LRK53_RS02460 0.58 +0.4 566,356 - LRK53_RS02460 0.58 -0.7 566,356 - LRK53_RS02460 0.58 +0.3 566,356 - LRK53_RS02460 0.58 -0.8 566,356 - LRK53_RS02460 0.58 -1.2 566,356 - LRK53_RS02460 0.58 -2.2 566,395 - LRK53_RS02460 0.61 -0.6 566,395 - LRK53_RS02460 0.61 -0.6 566,481 + LRK53_RS02460 0.67 +1.0 566,616 + LRK53_RS02460 0.77 -1.6 566,616 + LRK53_RS02460 0.77 -0.6 566,616 + LRK53_RS02460 0.77 -2.6 566,617 - LRK53_RS02460 0.77 -0.8 566,736 + LRK53_RS02460 0.86 +1.0 566,951 + -0.6 566,952 - +0.4 566,952 - -1.3 566,959 + -2.8 566,972 + -0.3 566,972 + -2.3 566,973 - -1.0 566,973 - -0.6 566,973 - -1.3 566,973 - +2.0 566,973 - -1.5 566,973 - +1.0 566,973 - -1.0 567,062 + +0.3 567,062 + +0.2 567,062 + -1.5 567,062 + -0.6 567,062 + +1.0 567,062 + +0.0 567,062 + -2.0 567,062 + -2.1 567,062 + -0.3 567,062 + +1.0 567,062 + -0.2 567,063 - -1.8 567,063 - -0.3 567,063 - -0.8 567,063 - -0.6 567,063 - -0.9 567,063 - +0.0 567,063 - -1.8 567,063 - +2.0 567,063 - +0.0 567,063 - -0.6 567,063 - -0.8 567,063 - -1.0 567,088 + +0.0 567,089 - -0.6 567,304 + LRK53_RS02465 0.23 +0.0 567,304 + LRK53_RS02465 0.23 +0.4 567,304 + LRK53_RS02465 0.23 -0.8 567,305 - LRK53_RS02465 0.23 +0.0 567,305 - LRK53_RS02465 0.23 -2.7 567,305 - LRK53_RS02465 0.23 +1.0 567,305 - LRK53_RS02465 0.23 +1.0 567,313 + LRK53_RS02465 0.24 +0.0 567,314 - LRK53_RS02465 0.24 +2.0 567,314 - LRK53_RS02465 0.24 +1.0 567,619 + LRK53_RS02465 0.52 -0.3 567,619 + LRK53_RS02465 0.52 -1.7 567,619 + LRK53_RS02465 0.52 -2.1 567,619 + LRK53_RS02465 0.52 -1.6 567,619 + LRK53_RS02465 0.52 -1.9 567,620 - LRK53_RS02465 0.52 -1.8 567,620 - LRK53_RS02465 0.52 +0.0 567,620 - LRK53_RS02465 0.52 -0.6 567,620 - LRK53_RS02465 0.52 -1.3 567,620 - LRK53_RS02465 0.52 -1.4 567,620 - LRK53_RS02465 0.52 -1.4 567,620 - LRK53_RS02465 0.52 -0.6 567,718 + LRK53_RS02465 0.61 -1.8 567,718 + LRK53_RS02465 0.61 -1.7 567,718 + LRK53_RS02465 0.61 -1.0 567,718 + LRK53_RS02465 0.61 -0.6 567,718 + LRK53_RS02465 0.61 -1.5 567,718 + LRK53_RS02465 0.61 -1.2 567,719 - LRK53_RS02465 0.61 -1.8 567,719 - LRK53_RS02465 0.61 -0.6 567,719 - LRK53_RS02465 0.61 +0.0 568,182 + -1.6 568,182 + -1.2 568,182 + -0.6 568,182 + +0.0 568,182 + +0.0 568,182 + +0.0 568,182 + -1.6 568,182 + +2.0 568,207 + -1.0 568,207 + -1.0 568,207 + +1.0
Or see this region's nucleotide sequence