Experiment: R2A_PIPES with Nickel (II) chloride 848 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS01490 and LRK53_RS01495 overlap by 1 nucleotides LRK53_RS01495 and LRK53_RS01500 are separated by 190 nucleotides
LRK53_RS01490: LRK53_RS01490 - glutathionylspermidine synthase family protein, at 356,164 to 357,342
_RS01490
LRK53_RS01495: LRK53_RS01495 - DUF1190 domain-containing protein, at 357,342 to 357,884
_RS01495
LRK53_RS01500: LRK53_RS01500 - serine/threonine protein kinase, at 358,075 to 359,037
_RS01500
Position (kb) 357
358 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 356.364 kb on - strand, within LRK53_RS01490 at 356.603 kb on + strand, within LRK53_RS01490 at 356.604 kb on - strand, within LRK53_RS01490 at 356.604 kb on - strand, within LRK53_RS01490 at 356.643 kb on - strand, within LRK53_RS01490 at 356.771 kb on + strand, within LRK53_RS01490 at 356.771 kb on + strand, within LRK53_RS01490 at 356.771 kb on + strand, within LRK53_RS01490 at 356.771 kb on + strand, within LRK53_RS01490 at 356.771 kb on + strand, within LRK53_RS01490 at 356.772 kb on - strand, within LRK53_RS01490 at 356.772 kb on - strand, within LRK53_RS01490 at 356.772 kb on - strand, within LRK53_RS01490 at 356.772 kb on - strand, within LRK53_RS01490 at 356.772 kb on - strand, within LRK53_RS01490 at 356.876 kb on + strand, within LRK53_RS01490 at 356.876 kb on + strand, within LRK53_RS01490 at 356.876 kb on + strand, within LRK53_RS01490 at 356.876 kb on + strand, within LRK53_RS01490 at 356.876 kb on + strand, within LRK53_RS01490 at 356.876 kb on + strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.877 kb on - strand, within LRK53_RS01490 at 356.987 kb on + strand, within LRK53_RS01490 at 356.987 kb on + strand, within LRK53_RS01490 at 356.987 kb on + strand, within LRK53_RS01490 at 356.987 kb on + strand, within LRK53_RS01490 at 356.987 kb on + strand, within LRK53_RS01490 at 356.987 kb on + strand, within LRK53_RS01490 at 356.987 kb on + strand, within LRK53_RS01490 at 356.988 kb on - strand, within LRK53_RS01490 at 356.988 kb on - strand, within LRK53_RS01490 at 356.988 kb on - strand, within LRK53_RS01490 at 356.988 kb on - strand, within LRK53_RS01490 at 356.988 kb on - strand, within LRK53_RS01490 at 356.988 kb on - strand, within LRK53_RS01490 at 356.999 kb on + strand, within LRK53_RS01490 at 356.999 kb on + strand, within LRK53_RS01490 at 356.999 kb on + strand, within LRK53_RS01490 at 357.000 kb on - strand, within LRK53_RS01490 at 357.000 kb on - strand, within LRK53_RS01490 at 357.026 kb on + strand, within LRK53_RS01490 at 357.026 kb on + strand, within LRK53_RS01490 at 357.047 kb on + strand, within LRK53_RS01490 at 357.047 kb on + strand, within LRK53_RS01490 at 357.048 kb on - strand, within LRK53_RS01490 at 357.053 kb on + strand, within LRK53_RS01490 at 357.054 kb on - strand, within LRK53_RS01490 at 357.054 kb on - strand, within LRK53_RS01490 at 357.054 kb on - strand, within LRK53_RS01490 at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.230 kb on + strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.231 kb on - strand at 357.233 kb on + strand at 357.233 kb on + strand at 357.233 kb on + strand at 357.233 kb on + strand at 357.233 kb on + strand at 357.233 kb on + strand at 357.234 kb on - strand at 357.234 kb on - strand at 357.251 kb on + strand at 357.653 kb on - strand, within LRK53_RS01495 at 357.653 kb on - strand, within LRK53_RS01495 at 357.740 kb on + strand, within LRK53_RS01495 at 357.740 kb on + strand, within LRK53_RS01495 at 357.740 kb on + strand, within LRK53_RS01495 at 357.741 kb on - strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.784 kb on + strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.785 kb on - strand, within LRK53_RS01495 at 357.966 kb on - strand at 357.966 kb on - strand at 358.060 kb on + strand at 358.060 kb on + strand at 358.060 kb on + strand at 358.076 kb on + strand at 358.076 kb on + strand at 358.076 kb on + strand at 358.076 kb on + strand at 358.076 kb on + strand at 358.077 kb on - strand at 358.077 kb on - strand at 358.077 kb on - strand at 358.077 kb on - strand at 358.202 kb on + strand, within LRK53_RS01500 at 358.203 kb on - strand, within LRK53_RS01500 at 358.441 kb on + strand, within LRK53_RS01500 at 358.442 kb on - strand, within LRK53_RS01500 at 358.505 kb on + strand, within LRK53_RS01500 at 358.505 kb on + strand, within LRK53_RS01500 at 358.505 kb on + strand, within LRK53_RS01500 at 358.505 kb on + strand, within LRK53_RS01500 at 358.505 kb on + strand, within LRK53_RS01500 at 358.505 kb on + strand, within LRK53_RS01500 at 358.506 kb on - strand, within LRK53_RS01500 at 358.506 kb on - strand, within LRK53_RS01500 at 358.506 kb on - strand, within LRK53_RS01500 at 358.506 kb on - strand, within LRK53_RS01500 at 358.506 kb on - strand, within LRK53_RS01500 at 358.506 kb on - strand, within LRK53_RS01500 at 358.506 kb on - strand, within LRK53_RS01500 at 358.856 kb on + strand, within LRK53_RS01500 at 358.856 kb on + strand, within LRK53_RS01500 at 358.857 kb on - strand, within LRK53_RS01500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 848 uM remove 356,364 - LRK53_RS01490 0.17 -1.0 356,603 + LRK53_RS01490 0.37 -1.5 356,604 - LRK53_RS01490 0.37 -0.7 356,604 - LRK53_RS01490 0.37 -0.3 356,643 - LRK53_RS01490 0.41 -0.6 356,771 + LRK53_RS01490 0.51 +0.0 356,771 + LRK53_RS01490 0.51 +1.0 356,771 + LRK53_RS01490 0.51 +1.0 356,771 + LRK53_RS01490 0.51 +1.0 356,771 + LRK53_RS01490 0.51 -0.4 356,772 - LRK53_RS01490 0.52 -1.3 356,772 - LRK53_RS01490 0.52 -1.1 356,772 - LRK53_RS01490 0.52 -1.9 356,772 - LRK53_RS01490 0.52 -1.2 356,772 - LRK53_RS01490 0.52 -2.0 356,876 + LRK53_RS01490 0.60 +1.0 356,876 + LRK53_RS01490 0.60 -1.8 356,876 + LRK53_RS01490 0.60 +0.8 356,876 + LRK53_RS01490 0.60 -1.3 356,876 + LRK53_RS01490 0.60 -0.7 356,876 + LRK53_RS01490 0.60 -1.7 356,877 - LRK53_RS01490 0.60 -2.1 356,877 - LRK53_RS01490 0.60 +0.7 356,877 - LRK53_RS01490 0.60 -1.0 356,877 - LRK53_RS01490 0.60 -2.8 356,877 - LRK53_RS01490 0.60 -1.6 356,877 - LRK53_RS01490 0.60 -0.6 356,877 - LRK53_RS01490 0.60 -0.2 356,877 - LRK53_RS01490 0.60 -0.3 356,877 - LRK53_RS01490 0.60 +1.0 356,877 - LRK53_RS01490 0.60 -0.3 356,987 + LRK53_RS01490 0.70 +0.0 356,987 + LRK53_RS01490 0.70 +0.0 356,987 + LRK53_RS01490 0.70 -0.6 356,987 + LRK53_RS01490 0.70 -0.6 356,987 + LRK53_RS01490 0.70 +0.0 356,987 + LRK53_RS01490 0.70 -0.6 356,987 + LRK53_RS01490 0.70 -1.0 356,988 - LRK53_RS01490 0.70 -1.1 356,988 - LRK53_RS01490 0.70 +2.0 356,988 - LRK53_RS01490 0.70 -0.6 356,988 - LRK53_RS01490 0.70 -1.0 356,988 - LRK53_RS01490 0.70 +1.0 356,988 - LRK53_RS01490 0.70 +0.0 356,999 + LRK53_RS01490 0.71 -0.4 356,999 + LRK53_RS01490 0.71 -0.8 356,999 + LRK53_RS01490 0.71 -0.3 357,000 - LRK53_RS01490 0.71 +0.0 357,000 - LRK53_RS01490 0.71 -1.0 357,026 + LRK53_RS01490 0.73 -0.6 357,026 + LRK53_RS01490 0.73 -1.8 357,047 + LRK53_RS01490 0.75 +0.7 357,047 + LRK53_RS01490 0.75 -1.6 357,048 - LRK53_RS01490 0.75 -0.8 357,053 + LRK53_RS01490 0.75 +1.0 357,054 - LRK53_RS01490 0.75 +0.2 357,054 - LRK53_RS01490 0.75 +1.0 357,054 - LRK53_RS01490 0.75 +1.0 357,230 + -2.1 357,230 + -1.0 357,230 + -0.6 357,230 + +0.0 357,230 + +0.4 357,230 + -0.3 357,230 + -0.2 357,230 + -0.6 357,230 + -0.4 357,230 + -0.6 357,230 + -0.3 357,230 + +0.0 357,230 + -0.6 357,231 - +1.0 357,231 - +0.2 357,231 - -1.0 357,231 - -0.6 357,231 - -0.4 357,231 - -1.3 357,231 - -0.1 357,231 - -0.8 357,231 - -2.7 357,233 + -1.6 357,233 + -1.0 357,233 + -1.3 357,233 + -2.1 357,233 + +1.0 357,233 + -1.8 357,234 - -1.3 357,234 - -1.3 357,251 + +0.0 357,653 - LRK53_RS01495 0.57 -1.6 357,653 - LRK53_RS01495 0.57 -0.6 357,740 + LRK53_RS01495 0.73 -0.6 357,740 + LRK53_RS01495 0.73 +0.4 357,740 + LRK53_RS01495 0.73 -0.6 357,741 - LRK53_RS01495 0.73 -2.4 357,784 + LRK53_RS01495 0.81 +0.0 357,784 + LRK53_RS01495 0.81 -2.3 357,784 + LRK53_RS01495 0.81 +0.0 357,784 + LRK53_RS01495 0.81 -0.7 357,784 + LRK53_RS01495 0.81 +0.0 357,784 + LRK53_RS01495 0.81 +0.0 357,784 + LRK53_RS01495 0.81 -0.8 357,784 + LRK53_RS01495 0.81 -2.1 357,784 + LRK53_RS01495 0.81 +0.0 357,784 + LRK53_RS01495 0.81 -1.7 357,784 + LRK53_RS01495 0.81 +0.0 357,784 + LRK53_RS01495 0.81 -0.6 357,784 + LRK53_RS01495 0.81 +0.3 357,784 + LRK53_RS01495 0.81 -0.6 357,784 + LRK53_RS01495 0.81 -0.6 357,784 + LRK53_RS01495 0.81 +0.0 357,784 + LRK53_RS01495 0.81 -1.3 357,785 - LRK53_RS01495 0.82 -0.6 357,785 - LRK53_RS01495 0.82 -0.6 357,785 - LRK53_RS01495 0.82 -1.3 357,785 - LRK53_RS01495 0.82 -1.3 357,785 - LRK53_RS01495 0.82 +1.0 357,785 - LRK53_RS01495 0.82 -1.3 357,785 - LRK53_RS01495 0.82 +0.0 357,785 - LRK53_RS01495 0.82 -1.3 357,785 - LRK53_RS01495 0.82 -1.0 357,785 - LRK53_RS01495 0.82 -1.8 357,785 - LRK53_RS01495 0.82 -1.0 357,785 - LRK53_RS01495 0.82 -1.6 357,785 - LRK53_RS01495 0.82 -1.6 357,785 - LRK53_RS01495 0.82 -1.8 357,966 - -2.1 357,966 - -0.4 358,060 + +1.0 358,060 + -2.0 358,060 + -1.7 358,076 + -2.1 358,076 + -0.6 358,076 + -1.3 358,076 + +1.0 358,076 + +0.4 358,077 - +0.0 358,077 - -1.8 358,077 - -0.7 358,077 - -0.8 358,202 + LRK53_RS01500 0.13 +1.0 358,203 - LRK53_RS01500 0.13 -1.0 358,441 + LRK53_RS01500 0.38 +1.0 358,442 - LRK53_RS01500 0.38 +0.0 358,505 + LRK53_RS01500 0.45 +0.0 358,505 + LRK53_RS01500 0.45 -1.0 358,505 + LRK53_RS01500 0.45 +0.7 358,505 + LRK53_RS01500 0.45 -1.8 358,505 + LRK53_RS01500 0.45 -1.0 358,505 + LRK53_RS01500 0.45 +1.0 358,506 - LRK53_RS01500 0.45 -2.5 358,506 - LRK53_RS01500 0.45 +0.2 358,506 - LRK53_RS01500 0.45 -2.0 358,506 - LRK53_RS01500 0.45 +1.0 358,506 - LRK53_RS01500 0.45 -0.2 358,506 - LRK53_RS01500 0.45 -0.6 358,506 - LRK53_RS01500 0.45 +1.0 358,856 + LRK53_RS01500 0.81 -0.8 358,856 + LRK53_RS01500 0.81 -0.8 358,857 - LRK53_RS01500 0.81 +1.0
Or see this region's nucleotide sequence