Experiment: R2A_PIPES with Nickel (II) chloride 848 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00980 and LRK53_RS00985 are separated by 126 nucleotides LRK53_RS00985 and LRK53_RS00990 are separated by 90 nucleotides LRK53_RS00990 and LRK53_RS00995 are separated by 132 nucleotides
LRK53_RS00980: LRK53_RS00980 - hypothetical protein, at 244,977 to 245,717
_RS00980
LRK53_RS00985: LRK53_RS00985 - hypothetical protein, at 245,844 to 246,236
_RS00985
LRK53_RS00990: LRK53_RS00990 - hypothetical protein, at 246,327 to 246,944
_RS00990
LRK53_RS00995: LRK53_RS00995 - hypothetical protein, at 247,077 to 247,523
_RS00995
Position (kb) 245
246
247 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 244.859 kb on + strand at 244.859 kb on + strand at 244.859 kb on + strand at 244.859 kb on + strand at 244.860 kb on - strand at 244.860 kb on - strand at 244.864 kb on + strand at 244.864 kb on + strand at 244.864 kb on + strand at 244.864 kb on + strand at 244.865 kb on - strand at 244.865 kb on - strand at 244.865 kb on - strand at 244.876 kb on + strand at 244.876 kb on + strand at 244.876 kb on + strand at 245.052 kb on + strand, within LRK53_RS00980 at 245.052 kb on + strand, within LRK53_RS00980 at 245.053 kb on - strand, within LRK53_RS00980 at 245.142 kb on - strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.207 kb on + strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.208 kb on - strand, within LRK53_RS00980 at 245.211 kb on + strand, within LRK53_RS00980 at 245.211 kb on + strand, within LRK53_RS00980 at 245.211 kb on + strand, within LRK53_RS00980 at 245.211 kb on + strand, within LRK53_RS00980 at 245.211 kb on + strand, within LRK53_RS00980 at 245.211 kb on + strand, within LRK53_RS00980 at 245.212 kb on - strand, within LRK53_RS00980 at 245.212 kb on - strand, within LRK53_RS00980 at 245.212 kb on - strand, within LRK53_RS00980 at 245.212 kb on - strand, within LRK53_RS00980 at 245.212 kb on - strand, within LRK53_RS00980 at 245.259 kb on - strand, within LRK53_RS00980 at 245.259 kb on - strand, within LRK53_RS00980 at 245.259 kb on - strand, within LRK53_RS00980 at 245.259 kb on - strand, within LRK53_RS00980 at 245.334 kb on - strand, within LRK53_RS00980 at 245.402 kb on - strand, within LRK53_RS00980 at 245.468 kb on + strand, within LRK53_RS00980 at 245.469 kb on - strand, within LRK53_RS00980 at 245.469 kb on - strand, within LRK53_RS00980 at 245.469 kb on - strand, within LRK53_RS00980 at 245.486 kb on + strand, within LRK53_RS00980 at 245.501 kb on + strand, within LRK53_RS00980 at 245.502 kb on - strand, within LRK53_RS00980 at 245.502 kb on - strand, within LRK53_RS00980 at 245.513 kb on + strand, within LRK53_RS00980 at 245.513 kb on + strand, within LRK53_RS00980 at 245.513 kb on + strand, within LRK53_RS00980 at 245.513 kb on + strand, within LRK53_RS00980 at 245.514 kb on - strand, within LRK53_RS00980 at 245.514 kb on - strand, within LRK53_RS00980 at 245.567 kb on - strand, within LRK53_RS00980 at 245.611 kb on + strand, within LRK53_RS00980 at 245.611 kb on + strand, within LRK53_RS00980 at 245.611 kb on + strand, within LRK53_RS00980 at 245.611 kb on + strand, within LRK53_RS00980 at 245.611 kb on + strand, within LRK53_RS00980 at 245.611 kb on + strand, within LRK53_RS00980 at 245.612 kb on - strand, within LRK53_RS00980 at 245.612 kb on - strand, within LRK53_RS00980 at 245.698 kb on + strand at 245.711 kb on + strand at 245.712 kb on - strand at 245.712 kb on - strand at 245.715 kb on + strand at 245.716 kb on - strand at 245.726 kb on - strand at 245.749 kb on + strand at 245.750 kb on - strand at 245.750 kb on - strand at 245.750 kb on - strand at 245.785 kb on + strand at 245.785 kb on + strand at 245.792 kb on + strand at 245.792 kb on + strand at 245.793 kb on - strand at 245.793 kb on - strand at 245.793 kb on - strand at 245.811 kb on + strand at 245.812 kb on - strand at 245.822 kb on + strand at 245.984 kb on - strand, within LRK53_RS00985 at 246.033 kb on - strand, within LRK53_RS00985 at 246.033 kb on - strand, within LRK53_RS00985 at 246.069 kb on - strand, within LRK53_RS00985 at 246.069 kb on - strand, within LRK53_RS00985 at 246.083 kb on + strand, within LRK53_RS00985 at 246.084 kb on - strand, within LRK53_RS00985 at 246.105 kb on + strand, within LRK53_RS00985 at 246.131 kb on + strand, within LRK53_RS00985 at 246.131 kb on + strand, within LRK53_RS00985 at 246.131 kb on + strand, within LRK53_RS00985 at 246.145 kb on + strand, within LRK53_RS00985 at 246.145 kb on + strand, within LRK53_RS00985 at 246.145 kb on + strand, within LRK53_RS00985 at 246.146 kb on - strand, within LRK53_RS00985 at 246.203 kb on - strand at 246.223 kb on + strand at 246.223 kb on + strand at 246.224 kb on - strand at 246.241 kb on + strand at 246.242 kb on - strand at 246.242 kb on - strand at 246.242 kb on - strand at 246.284 kb on + strand at 246.285 kb on - strand at 246.285 kb on - strand at 246.285 kb on - strand at 246.312 kb on + strand at 246.312 kb on + strand at 246.338 kb on + strand at 246.338 kb on + strand at 246.338 kb on + strand at 246.338 kb on + strand at 246.338 kb on + strand at 246.339 kb on - strand at 246.339 kb on - strand at 246.340 kb on + strand at 246.341 kb on - strand at 246.341 kb on - strand at 246.341 kb on - strand at 246.341 kb on - strand at 246.350 kb on + strand at 246.350 kb on + strand at 246.350 kb on + strand at 246.351 kb on - strand at 246.467 kb on + strand, within LRK53_RS00990 at 246.467 kb on + strand, within LRK53_RS00990 at 246.467 kb on + strand, within LRK53_RS00990 at 246.467 kb on + strand, within LRK53_RS00990 at 246.467 kb on + strand, within LRK53_RS00990 at 246.468 kb on - strand, within LRK53_RS00990 at 246.468 kb on - strand, within LRK53_RS00990 at 246.468 kb on - strand, within LRK53_RS00990 at 246.468 kb on - strand, within LRK53_RS00990 at 246.468 kb on - strand, within LRK53_RS00990 at 246.471 kb on + strand, within LRK53_RS00990 at 246.471 kb on + strand, within LRK53_RS00990 at 246.471 kb on + strand, within LRK53_RS00990 at 246.471 kb on + strand, within LRK53_RS00990 at 246.471 kb on + strand, within LRK53_RS00990 at 246.472 kb on - strand, within LRK53_RS00990 at 246.544 kb on + strand, within LRK53_RS00990 at 246.545 kb on - strand, within LRK53_RS00990 at 246.576 kb on + strand, within LRK53_RS00990 at 246.576 kb on + strand, within LRK53_RS00990 at 246.576 kb on + strand, within LRK53_RS00990 at 246.577 kb on - strand, within LRK53_RS00990 at 246.577 kb on - strand, within LRK53_RS00990 at 246.577 kb on - strand, within LRK53_RS00990 at 246.577 kb on - strand, within LRK53_RS00990 at 246.623 kb on + strand, within LRK53_RS00990 at 246.634 kb on + strand, within LRK53_RS00990 at 246.635 kb on - strand, within LRK53_RS00990 at 246.641 kb on + strand, within LRK53_RS00990 at 246.662 kb on - strand, within LRK53_RS00990 at 246.681 kb on + strand, within LRK53_RS00990 at 246.700 kb on + strand, within LRK53_RS00990 at 246.706 kb on + strand, within LRK53_RS00990 at 246.707 kb on - strand, within LRK53_RS00990 at 246.744 kb on - strand, within LRK53_RS00990 at 246.744 kb on - strand, within LRK53_RS00990 at 246.749 kb on + strand, within LRK53_RS00990 at 246.818 kb on - strand, within LRK53_RS00990 at 246.841 kb on + strand, within LRK53_RS00990 at 246.841 kb on + strand, within LRK53_RS00990 at 246.842 kb on - strand, within LRK53_RS00990 at 246.842 kb on - strand, within LRK53_RS00990 at 246.865 kb on + strand, within LRK53_RS00990 at 246.874 kb on + strand, within LRK53_RS00990 at 246.875 kb on - strand, within LRK53_RS00990 at 246.894 kb on + strand at 246.894 kb on + strand at 246.894 kb on + strand at 246.894 kb on + strand at 246.894 kb on + strand at 246.895 kb on - strand at 246.895 kb on - strand at 246.895 kb on - strand at 246.901 kb on + strand at 246.956 kb on + strand at 246.956 kb on + strand at 246.993 kb on - strand at 246.993 kb on - strand at 246.993 kb on - strand at 246.998 kb on - strand at 246.998 kb on - strand at 247.003 kb on + strand at 247.003 kb on + strand at 247.007 kb on + strand at 247.007 kb on + strand at 247.007 kb on + strand at 247.007 kb on + strand at 247.007 kb on + strand at 247.007 kb on + strand at 247.008 kb on - strand at 247.008 kb on - strand at 247.008 kb on - strand at 247.008 kb on - strand at 247.032 kb on - strand at 247.032 kb on - strand at 247.032 kb on - strand at 247.032 kb on - strand at 247.076 kb on + strand at 247.076 kb on + strand at 247.076 kb on + strand at 247.077 kb on - strand at 247.077 kb on - strand at 247.117 kb on + strand at 247.118 kb on - strand at 247.153 kb on + strand, within LRK53_RS00995 at 247.153 kb on + strand, within LRK53_RS00995 at 247.155 kb on + strand, within LRK53_RS00995 at 247.155 kb on + strand, within LRK53_RS00995 at 247.156 kb on - strand, within LRK53_RS00995 at 247.156 kb on - strand, within LRK53_RS00995 at 247.156 kb on - strand, within LRK53_RS00995 at 247.156 kb on - strand, within LRK53_RS00995 at 247.156 kb on - strand, within LRK53_RS00995 at 247.159 kb on + strand, within LRK53_RS00995 at 247.176 kb on + strand, within LRK53_RS00995 at 247.177 kb on - strand, within LRK53_RS00995 at 247.181 kb on - strand, within LRK53_RS00995 at 247.230 kb on + strand, within LRK53_RS00995 at 247.230 kb on + strand, within LRK53_RS00995 at 247.231 kb on - strand, within LRK53_RS00995
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 848 uM remove 244,859 + -1.3 244,859 + -1.1 244,859 + -1.5 244,859 + +0.5 244,860 - -0.7 244,860 - -1.8 244,864 + +1.1 244,864 + +0.1 244,864 + -1.1 244,864 + -2.1 244,865 - -1.7 244,865 - +0.1 244,865 - -2.8 244,876 + -1.1 244,876 + -1.3 244,876 + -0.3 245,052 + LRK53_RS00980 0.10 +2.1 245,052 + LRK53_RS00980 0.10 -1.7 245,053 - LRK53_RS00980 0.10 -0.5 245,142 - LRK53_RS00980 0.22 -1.9 245,207 + LRK53_RS00980 0.31 -0.3 245,207 + LRK53_RS00980 0.31 -1.7 245,207 + LRK53_RS00980 0.31 +0.1 245,207 + LRK53_RS00980 0.31 -0.7 245,207 + LRK53_RS00980 0.31 -1.3 245,207 + LRK53_RS00980 0.31 -0.9 245,207 + LRK53_RS00980 0.31 -0.5 245,207 + LRK53_RS00980 0.31 -1.6 245,207 + LRK53_RS00980 0.31 -1.9 245,207 + LRK53_RS00980 0.31 +0.8 245,208 - LRK53_RS00980 0.31 -0.9 245,208 - LRK53_RS00980 0.31 -0.5 245,208 - LRK53_RS00980 0.31 +0.1 245,208 - LRK53_RS00980 0.31 +0.1 245,208 - LRK53_RS00980 0.31 -1.5 245,208 - LRK53_RS00980 0.31 +0.1 245,208 - LRK53_RS00980 0.31 -2.7 245,208 - LRK53_RS00980 0.31 -0.8 245,208 - LRK53_RS00980 0.31 -0.5 245,211 + LRK53_RS00980 0.32 -0.5 245,211 + LRK53_RS00980 0.32 -0.2 245,211 + LRK53_RS00980 0.32 -1.3 245,211 + LRK53_RS00980 0.32 +1.1 245,211 + LRK53_RS00980 0.32 -0.3 245,211 + LRK53_RS00980 0.32 -1.3 245,212 - LRK53_RS00980 0.32 -0.3 245,212 - LRK53_RS00980 0.32 -0.0 245,212 - LRK53_RS00980 0.32 -0.3 245,212 - LRK53_RS00980 0.32 +0.1 245,212 - LRK53_RS00980 0.32 -1.5 245,259 - LRK53_RS00980 0.38 -1.3 245,259 - LRK53_RS00980 0.38 -0.7 245,259 - LRK53_RS00980 0.38 -0.5 245,259 - LRK53_RS00980 0.38 -2.2 245,334 - LRK53_RS00980 0.48 -1.4 245,402 - LRK53_RS00980 0.57 +0.1 245,468 + LRK53_RS00980 0.66 -1.0 245,469 - LRK53_RS00980 0.66 -0.8 245,469 - LRK53_RS00980 0.66 -1.0 245,469 - LRK53_RS00980 0.66 -2.6 245,486 + LRK53_RS00980 0.69 -0.4 245,501 + LRK53_RS00980 0.71 -1.4 245,502 - LRK53_RS00980 0.71 -0.3 245,502 - LRK53_RS00980 0.71 +0.1 245,513 + LRK53_RS00980 0.72 -0.2 245,513 + LRK53_RS00980 0.72 -0.1 245,513 + LRK53_RS00980 0.72 +1.6 245,513 + LRK53_RS00980 0.72 -0.5 245,514 - LRK53_RS00980 0.72 +1.1 245,514 - LRK53_RS00980 0.72 -1.7 245,567 - LRK53_RS00980 0.80 +0.1 245,611 + LRK53_RS00980 0.86 -1.9 245,611 + LRK53_RS00980 0.86 -0.3 245,611 + LRK53_RS00980 0.86 -2.4 245,611 + LRK53_RS00980 0.86 -0.5 245,611 + LRK53_RS00980 0.86 -0.5 245,611 + LRK53_RS00980 0.86 +0.1 245,612 - LRK53_RS00980 0.86 -0.9 245,612 - LRK53_RS00980 0.86 -1.8 245,698 + -1.1 245,711 + -0.6 245,712 - -0.3 245,712 - -0.7 245,715 + +0.3 245,716 - -0.5 245,726 - -0.8 245,749 + -0.5 245,750 - -0.8 245,750 - -2.4 245,750 - -0.9 245,785 + -1.6 245,785 + -1.1 245,792 + +0.1 245,792 + -1.5 245,793 - -0.9 245,793 - +0.1 245,793 - -0.9 245,811 + -0.5 245,812 - +1.1 245,822 + -0.9 245,984 - LRK53_RS00985 0.36 -1.8 246,033 - LRK53_RS00985 0.48 -0.5 246,033 - LRK53_RS00985 0.48 -1.3 246,069 - LRK53_RS00985 0.57 -1.5 246,069 - LRK53_RS00985 0.57 -0.9 246,083 + LRK53_RS00985 0.61 -3.3 246,084 - LRK53_RS00985 0.61 -0.5 246,105 + LRK53_RS00985 0.66 -2.3 246,131 + LRK53_RS00985 0.73 -1.3 246,131 + LRK53_RS00985 0.73 -0.5 246,131 + LRK53_RS00985 0.73 +0.1 246,145 + LRK53_RS00985 0.77 -0.5 246,145 + LRK53_RS00985 0.77 -0.5 246,145 + LRK53_RS00985 0.77 +1.1 246,146 - LRK53_RS00985 0.77 -2.1 246,203 - -1.6 246,223 + +1.5 246,223 + -1.1 246,224 - -1.1 246,241 + -0.9 246,242 - -0.5 246,242 - -0.7 246,242 - -0.5 246,284 + -1.5 246,285 - -0.5 246,285 - -0.9 246,285 - -0.5 246,312 + +0.1 246,312 + +0.1 246,338 + -1.6 246,338 + +0.1 246,338 + -2.6 246,338 + -1.3 246,338 + +0.1 246,339 - -0.9 246,339 - -0.3 246,340 + -1.3 246,341 - -0.5 246,341 - -1.1 246,341 - -0.5 246,341 - -1.8 246,350 + -1.9 246,350 + -0.5 246,350 + -0.4 246,351 - +0.7 246,467 + LRK53_RS00990 0.23 -1.5 246,467 + LRK53_RS00990 0.23 -0.9 246,467 + LRK53_RS00990 0.23 -2.3 246,467 + LRK53_RS00990 0.23 -1.3 246,467 + LRK53_RS00990 0.23 -0.3 246,468 - LRK53_RS00990 0.23 -2.3 246,468 - LRK53_RS00990 0.23 -0.9 246,468 - LRK53_RS00990 0.23 +2.1 246,468 - LRK53_RS00990 0.23 -0.4 246,468 - LRK53_RS00990 0.23 -0.6 246,471 + LRK53_RS00990 0.23 -2.6 246,471 + LRK53_RS00990 0.23 +0.1 246,471 + LRK53_RS00990 0.23 +0.1 246,471 + LRK53_RS00990 0.23 -2.5 246,471 + LRK53_RS00990 0.23 +1.1 246,472 - LRK53_RS00990 0.23 -0.9 246,544 + LRK53_RS00990 0.35 -1.6 246,545 - LRK53_RS00990 0.35 -0.9 246,576 + LRK53_RS00990 0.40 +0.1 246,576 + LRK53_RS00990 0.40 -1.5 246,576 + LRK53_RS00990 0.40 -0.8 246,577 - LRK53_RS00990 0.40 -0.3 246,577 - LRK53_RS00990 0.40 -0.7 246,577 - LRK53_RS00990 0.40 -0.2 246,577 - LRK53_RS00990 0.40 -1.2 246,623 + LRK53_RS00990 0.48 -3.1 246,634 + LRK53_RS00990 0.50 +0.1 246,635 - LRK53_RS00990 0.50 +0.1 246,641 + LRK53_RS00990 0.51 +0.3 246,662 - LRK53_RS00990 0.54 +0.5 246,681 + LRK53_RS00990 0.57 -0.3 246,700 + LRK53_RS00990 0.60 -0.3 246,706 + LRK53_RS00990 0.61 -0.5 246,707 - LRK53_RS00990 0.61 +1.2 246,744 - LRK53_RS00990 0.67 -0.2 246,744 - LRK53_RS00990 0.67 -1.5 246,749 + LRK53_RS00990 0.68 -0.5 246,818 - LRK53_RS00990 0.79 -0.5 246,841 + LRK53_RS00990 0.83 +0.5 246,841 + LRK53_RS00990 0.83 +0.5 246,842 - LRK53_RS00990 0.83 -0.9 246,842 - LRK53_RS00990 0.83 +0.5 246,865 + LRK53_RS00990 0.87 -0.7 246,874 + LRK53_RS00990 0.89 -0.7 246,875 - LRK53_RS00990 0.89 -0.9 246,894 + -1.3 246,894 + -0.5 246,894 + -1.7 246,894 + -0.5 246,894 + +1.1 246,895 - -1.6 246,895 - -1.3 246,895 - -0.2 246,901 + -0.3 246,956 + +0.1 246,956 + +0.5 246,993 - -2.3 246,993 - +0.3 246,993 - -0.5 246,998 - +0.2 246,998 - +0.3 247,003 + -0.7 247,003 + -1.4 247,007 + +0.1 247,007 + -1.1 247,007 + +0.1 247,007 + -0.9 247,007 + -0.7 247,007 + -1.3 247,008 - +0.5 247,008 - +0.1 247,008 - +0.5 247,008 - -0.9 247,032 - +0.8 247,032 - +1.4 247,032 - -1.3 247,032 - -2.1 247,076 + -0.7 247,076 + -1.4 247,076 + -0.8 247,077 - -0.3 247,077 - -0.9 247,117 + +1.7 247,118 - -0.9 247,153 + LRK53_RS00995 0.17 -1.5 247,153 + LRK53_RS00995 0.17 -1.3 247,155 + LRK53_RS00995 0.17 +1.9 247,155 + LRK53_RS00995 0.17 -1.8 247,156 - LRK53_RS00995 0.18 -0.9 247,156 - LRK53_RS00995 0.18 -0.7 247,156 - LRK53_RS00995 0.18 -1.3 247,156 - LRK53_RS00995 0.18 +0.3 247,156 - LRK53_RS00995 0.18 -1.9 247,159 + LRK53_RS00995 0.18 +0.1 247,176 + LRK53_RS00995 0.22 -1.2 247,177 - LRK53_RS00995 0.22 -1.3 247,181 - LRK53_RS00995 0.23 -0.5 247,230 + LRK53_RS00995 0.34 -0.7 247,230 + LRK53_RS00995 0.34 -1.8 247,231 - LRK53_RS00995 0.34 +1.1
Or see this region's nucleotide sequence