Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07925 and LRK53_RS07930 are separated by 133 nucleotides LRK53_RS07930 and LRK53_RS07935 are separated by 166 nucleotides
LRK53_RS07925: LRK53_RS07925 - metalloregulator ArsR/SmtB family transcription factor, at 1,772,661 to 1,773,590
_RS07925
LRK53_RS07930: LRK53_RS07930 - acyl-CoA dehydrogenase family protein, at 1,773,724 to 1,774,884
_RS07930
LRK53_RS07935: LRK53_RS07935 - multidrug efflux RND transporter permease subunit, at 1,775,051 to 1,778,299
_RS07935
Position (kb) 1773
1774
1775 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1772.767 kb on + strand, within LRK53_RS07925 at 1772.768 kb on - strand, within LRK53_RS07925 at 1772.926 kb on + strand, within LRK53_RS07925 at 1772.926 kb on + strand, within LRK53_RS07925 at 1773.208 kb on + strand, within LRK53_RS07925 at 1773.208 kb on + strand, within LRK53_RS07925 at 1773.208 kb on + strand, within LRK53_RS07925 at 1773.208 kb on + strand, within LRK53_RS07925 at 1773.209 kb on - strand, within LRK53_RS07925 at 1773.209 kb on - strand, within LRK53_RS07925 at 1773.209 kb on - strand, within LRK53_RS07925 at 1773.383 kb on - strand, within LRK53_RS07925 at 1773.383 kb on - strand, within LRK53_RS07925 at 1773.383 kb on - strand, within LRK53_RS07925 at 1773.383 kb on - strand, within LRK53_RS07925 at 1773.385 kb on + strand, within LRK53_RS07925 at 1773.385 kb on + strand, within LRK53_RS07925 at 1773.385 kb on + strand, within LRK53_RS07925 at 1773.386 kb on - strand, within LRK53_RS07925 at 1773.386 kb on - strand, within LRK53_RS07925 at 1773.495 kb on + strand, within LRK53_RS07925 at 1773.604 kb on - strand at 1773.621 kb on + strand at 1773.621 kb on + strand at 1773.621 kb on + strand at 1773.622 kb on - strand at 1773.622 kb on - strand at 1773.622 kb on - strand at 1773.622 kb on - strand at 1773.622 kb on - strand at 1773.622 kb on - strand at 1773.623 kb on + strand at 1773.624 kb on - strand at 1773.624 kb on - strand at 1773.629 kb on - strand at 1773.646 kb on - strand at 1773.646 kb on - strand at 1773.738 kb on + strand at 1773.898 kb on + strand, within LRK53_RS07930 at 1773.928 kb on + strand, within LRK53_RS07930 at 1773.928 kb on + strand, within LRK53_RS07930 at 1773.928 kb on + strand, within LRK53_RS07930 at 1773.928 kb on + strand, within LRK53_RS07930 at 1773.928 kb on + strand, within LRK53_RS07930 at 1773.928 kb on + strand, within LRK53_RS07930 at 1773.928 kb on + strand, within LRK53_RS07930 at 1773.929 kb on - strand, within LRK53_RS07930 at 1773.929 kb on - strand, within LRK53_RS07930 at 1773.929 kb on - strand, within LRK53_RS07930 at 1773.929 kb on - strand, within LRK53_RS07930 at 1773.929 kb on - strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.051 kb on + strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.052 kb on - strand, within LRK53_RS07930 at 1774.090 kb on + strand, within LRK53_RS07930 at 1774.091 kb on - strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.219 kb on + strand, within LRK53_RS07930 at 1774.220 kb on - strand, within LRK53_RS07930 at 1774.220 kb on - strand, within LRK53_RS07930 at 1774.220 kb on - strand, within LRK53_RS07930 at 1774.220 kb on - strand, within LRK53_RS07930 at 1774.220 kb on - strand, within LRK53_RS07930 at 1774.220 kb on - strand, within LRK53_RS07930 at 1774.268 kb on + strand, within LRK53_RS07930 at 1774.269 kb on - strand, within LRK53_RS07930 at 1774.375 kb on + strand, within LRK53_RS07930 at 1774.375 kb on + strand, within LRK53_RS07930 at 1774.375 kb on + strand, within LRK53_RS07930 at 1774.375 kb on + strand, within LRK53_RS07930 at 1774.376 kb on - strand, within LRK53_RS07930 at 1774.376 kb on - strand, within LRK53_RS07930 at 1774.376 kb on - strand, within LRK53_RS07930 at 1774.498 kb on + strand, within LRK53_RS07930 at 1774.765 kb on + strand, within LRK53_RS07930 at 1774.765 kb on + strand, within LRK53_RS07930 at 1774.765 kb on + strand, within LRK53_RS07930 at 1774.765 kb on + strand, within LRK53_RS07930 at 1774.766 kb on - strand, within LRK53_RS07930 at 1774.766 kb on - strand, within LRK53_RS07930 at 1774.792 kb on + strand at 1774.793 kb on - strand at 1774.793 kb on - strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.822 kb on + strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.823 kb on - strand at 1774.894 kb on + strand at 1774.894 kb on + strand at 1774.895 kb on - strand at 1774.895 kb on - strand at 1775.025 kb on + strand at 1775.025 kb on + strand at 1775.025 kb on + strand at 1775.026 kb on - strand at 1775.026 kb on - strand at 1775.032 kb on + strand at 1775.032 kb on + strand at 1775.032 kb on + strand at 1775.154 kb on + strand at 1775.154 kb on + strand at 1775.154 kb on + strand at 1775.154 kb on + strand at 1775.154 kb on + strand at 1775.154 kb on + strand at 1775.155 kb on - strand at 1775.155 kb on - strand at 1775.155 kb on - strand at 1775.155 kb on - strand at 1775.155 kb on - strand at 1775.155 kb on - strand at 1775.155 kb on - strand at 1775.155 kb on - strand at 1775.174 kb on + strand at 1775.174 kb on + strand at 1775.175 kb on - strand at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.451 kb on + strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.452 kb on - strand, within LRK53_RS07935 at 1775.553 kb on + strand, within LRK53_RS07935 at 1775.554 kb on - strand, within LRK53_RS07935 at 1775.554 kb on - strand, within LRK53_RS07935 at 1775.554 kb on - strand, within LRK53_RS07935 at 1775.554 kb on - strand, within LRK53_RS07935 at 1775.554 kb on - strand, within LRK53_RS07935 at 1775.554 kb on - strand, within LRK53_RS07935 at 1775.638 kb on - strand, within LRK53_RS07935 at 1775.638 kb on - strand, within LRK53_RS07935 at 1775.658 kb on + strand, within LRK53_RS07935
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 1,772,767 + LRK53_RS07925 0.11 +0.8 1,772,768 - LRK53_RS07925 0.12 +1.0 1,772,926 + LRK53_RS07925 0.28 -0.0 1,772,926 + LRK53_RS07925 0.28 -0.6 1,773,208 + LRK53_RS07925 0.59 -0.0 1,773,208 + LRK53_RS07925 0.59 -1.8 1,773,208 + LRK53_RS07925 0.59 +0.4 1,773,208 + LRK53_RS07925 0.59 -1.0 1,773,209 - LRK53_RS07925 0.59 +0.4 1,773,209 - LRK53_RS07925 0.59 -0.0 1,773,209 - LRK53_RS07925 0.59 +2.0 1,773,383 - LRK53_RS07925 0.78 -0.7 1,773,383 - LRK53_RS07925 0.78 -0.3 1,773,383 - LRK53_RS07925 0.78 -1.1 1,773,383 - LRK53_RS07925 0.78 +1.7 1,773,385 + LRK53_RS07925 0.78 -0.0 1,773,385 + LRK53_RS07925 0.78 -0.0 1,773,385 + LRK53_RS07925 0.78 -1.0 1,773,386 - LRK53_RS07925 0.78 -1.5 1,773,386 - LRK53_RS07925 0.78 -0.6 1,773,495 + LRK53_RS07925 0.90 -1.6 1,773,604 - -1.1 1,773,621 + -1.6 1,773,621 + -0.0 1,773,621 + -0.3 1,773,622 - -1.4 1,773,622 - -1.4 1,773,622 - -1.0 1,773,622 - +1.0 1,773,622 - -0.2 1,773,622 - -0.6 1,773,623 + +0.4 1,773,624 - -2.0 1,773,624 - -2.0 1,773,629 - +1.0 1,773,646 - -0.0 1,773,646 - -3.1 1,773,738 + -1.2 1,773,898 + LRK53_RS07930 0.15 -2.1 1,773,928 + LRK53_RS07930 0.18 +2.0 1,773,928 + LRK53_RS07930 0.18 -0.0 1,773,928 + LRK53_RS07930 0.18 -1.0 1,773,928 + LRK53_RS07930 0.18 +0.4 1,773,928 + LRK53_RS07930 0.18 -0.4 1,773,928 + LRK53_RS07930 0.18 -1.0 1,773,928 + LRK53_RS07930 0.18 -1.4 1,773,929 - LRK53_RS07930 0.18 -1.0 1,773,929 - LRK53_RS07930 0.18 -0.0 1,773,929 - LRK53_RS07930 0.18 +1.0 1,773,929 - LRK53_RS07930 0.18 -0.9 1,773,929 - LRK53_RS07930 0.18 -1.1 1,774,051 + LRK53_RS07930 0.28 -0.6 1,774,051 + LRK53_RS07930 0.28 -0.0 1,774,051 + LRK53_RS07930 0.28 -0.8 1,774,051 + LRK53_RS07930 0.28 +0.4 1,774,051 + LRK53_RS07930 0.28 -0.6 1,774,051 + LRK53_RS07930 0.28 -0.0 1,774,051 + LRK53_RS07930 0.28 -0.0 1,774,051 + LRK53_RS07930 0.28 -2.2 1,774,051 + LRK53_RS07930 0.28 -1.7 1,774,051 + LRK53_RS07930 0.28 -1.0 1,774,051 + LRK53_RS07930 0.28 +1.4 1,774,051 + LRK53_RS07930 0.28 -0.6 1,774,051 + LRK53_RS07930 0.28 +0.4 1,774,051 + LRK53_RS07930 0.28 -1.3 1,774,051 + LRK53_RS07930 0.28 -0.5 1,774,051 + LRK53_RS07930 0.28 -2.8 1,774,051 + LRK53_RS07930 0.28 -0.6 1,774,051 + LRK53_RS07930 0.28 -1.4 1,774,051 + LRK53_RS07930 0.28 +1.0 1,774,052 - LRK53_RS07930 0.28 -1.8 1,774,052 - LRK53_RS07930 0.28 +0.6 1,774,052 - LRK53_RS07930 0.28 -1.9 1,774,052 - LRK53_RS07930 0.28 -1.6 1,774,052 - LRK53_RS07930 0.28 -1.0 1,774,052 - LRK53_RS07930 0.28 -1.5 1,774,052 - LRK53_RS07930 0.28 +0.6 1,774,052 - LRK53_RS07930 0.28 -0.0 1,774,052 - LRK53_RS07930 0.28 -1.8 1,774,052 - LRK53_RS07930 0.28 -0.4 1,774,052 - LRK53_RS07930 0.28 -0.6 1,774,052 - LRK53_RS07930 0.28 -0.0 1,774,052 - LRK53_RS07930 0.28 +1.0 1,774,052 - LRK53_RS07930 0.28 +1.0 1,774,052 - LRK53_RS07930 0.28 -0.0 1,774,052 - LRK53_RS07930 0.28 -1.2 1,774,052 - LRK53_RS07930 0.28 -1.0 1,774,052 - LRK53_RS07930 0.28 -1.0 1,774,052 - LRK53_RS07930 0.28 -0.6 1,774,052 - LRK53_RS07930 0.28 +0.2 1,774,052 - LRK53_RS07930 0.28 -0.0 1,774,090 + LRK53_RS07930 0.32 -0.0 1,774,091 - LRK53_RS07930 0.32 +1.0 1,774,219 + LRK53_RS07930 0.43 -1.8 1,774,219 + LRK53_RS07930 0.43 -2.6 1,774,219 + LRK53_RS07930 0.43 -1.0 1,774,219 + LRK53_RS07930 0.43 +1.6 1,774,219 + LRK53_RS07930 0.43 -0.6 1,774,219 + LRK53_RS07930 0.43 -1.1 1,774,219 + LRK53_RS07930 0.43 -0.4 1,774,219 + LRK53_RS07930 0.43 -0.8 1,774,220 - LRK53_RS07930 0.43 -1.0 1,774,220 - LRK53_RS07930 0.43 -0.0 1,774,220 - LRK53_RS07930 0.43 -1.6 1,774,220 - LRK53_RS07930 0.43 -1.4 1,774,220 - LRK53_RS07930 0.43 -2.0 1,774,220 - LRK53_RS07930 0.43 -1.3 1,774,268 + LRK53_RS07930 0.47 -0.0 1,774,269 - LRK53_RS07930 0.47 -1.4 1,774,375 + LRK53_RS07930 0.56 +0.6 1,774,375 + LRK53_RS07930 0.56 -1.2 1,774,375 + LRK53_RS07930 0.56 -1.3 1,774,375 + LRK53_RS07930 0.56 -0.6 1,774,376 - LRK53_RS07930 0.56 -0.6 1,774,376 - LRK53_RS07930 0.56 +2.0 1,774,376 - LRK53_RS07930 0.56 +1.4 1,774,498 + LRK53_RS07930 0.67 +1.0 1,774,765 + LRK53_RS07930 0.90 -1.4 1,774,765 + LRK53_RS07930 0.90 -1.4 1,774,765 + LRK53_RS07930 0.90 -0.2 1,774,765 + LRK53_RS07930 0.90 -1.1 1,774,766 - LRK53_RS07930 0.90 -0.2 1,774,766 - LRK53_RS07930 0.90 -0.9 1,774,792 + -1.6 1,774,793 - +1.0 1,774,793 - -0.4 1,774,822 + +0.4 1,774,822 + -1.4 1,774,822 + +0.4 1,774,822 + -0.0 1,774,822 + -0.0 1,774,822 + -0.5 1,774,822 + -0.2 1,774,822 + -3.0 1,774,822 + +1.0 1,774,823 - -0.0 1,774,823 - -0.6 1,774,823 - -0.6 1,774,823 - -1.4 1,774,823 - -0.2 1,774,823 - -0.0 1,774,823 - +1.0 1,774,823 - -1.0 1,774,823 - -1.6 1,774,894 + -0.6 1,774,894 + +0.4 1,774,895 - -0.3 1,774,895 - -0.6 1,775,025 + -2.7 1,775,025 + -1.7 1,775,025 + -0.9 1,775,026 - -0.7 1,775,026 - -2.0 1,775,032 + -1.8 1,775,032 + -0.4 1,775,032 + -1.0 1,775,154 + -0.8 1,775,154 + -0.6 1,775,154 + -0.4 1,775,154 + +0.6 1,775,154 + -0.2 1,775,154 + -0.0 1,775,155 - -1.4 1,775,155 - +1.0 1,775,155 - -1.4 1,775,155 - -1.0 1,775,155 - -1.4 1,775,155 - -0.2 1,775,155 - -0.6 1,775,155 - -1.0 1,775,174 + -2.1 1,775,174 + +0.4 1,775,175 - +1.6 1,775,451 + LRK53_RS07935 0.12 -1.0 1,775,451 + LRK53_RS07935 0.12 +0.6 1,775,451 + LRK53_RS07935 0.12 -1.0 1,775,451 + LRK53_RS07935 0.12 -0.3 1,775,451 + LRK53_RS07935 0.12 +1.4 1,775,451 + LRK53_RS07935 0.12 +1.0 1,775,451 + LRK53_RS07935 0.12 -1.6 1,775,451 + LRK53_RS07935 0.12 -0.3 1,775,451 + LRK53_RS07935 0.12 +1.0 1,775,451 + LRK53_RS07935 0.12 -0.0 1,775,451 + LRK53_RS07935 0.12 +0.6 1,775,451 + LRK53_RS07935 0.12 +0.4 1,775,451 + LRK53_RS07935 0.12 -0.4 1,775,451 + LRK53_RS07935 0.12 -1.0 1,775,451 + LRK53_RS07935 0.12 +0.2 1,775,452 - LRK53_RS07935 0.12 -0.0 1,775,452 - LRK53_RS07935 0.12 +2.0 1,775,452 - LRK53_RS07935 0.12 +0.4 1,775,452 - LRK53_RS07935 0.12 -0.6 1,775,452 - LRK53_RS07935 0.12 -1.4 1,775,452 - LRK53_RS07935 0.12 -0.6 1,775,452 - LRK53_RS07935 0.12 -0.0 1,775,452 - LRK53_RS07935 0.12 +1.8 1,775,452 - LRK53_RS07935 0.12 -1.0 1,775,452 - LRK53_RS07935 0.12 -1.6 1,775,452 - LRK53_RS07935 0.12 -2.6 1,775,452 - LRK53_RS07935 0.12 -1.1 1,775,452 - LRK53_RS07935 0.12 -1.4 1,775,553 + LRK53_RS07935 0.15 +0.4 1,775,554 - LRK53_RS07935 0.15 -1.5 1,775,554 - LRK53_RS07935 0.15 +0.6 1,775,554 - LRK53_RS07935 0.15 -1.0 1,775,554 - LRK53_RS07935 0.15 +0.2 1,775,554 - LRK53_RS07935 0.15 -0.0 1,775,554 - LRK53_RS07935 0.15 -0.7 1,775,638 - LRK53_RS07935 0.18 -0.0 1,775,638 - LRK53_RS07935 0.18 +0.6 1,775,658 + LRK53_RS07935 0.19 -2.2
Or see this region's nucleotide sequence