Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03860 and LRK53_RS03865 are separated by 6 nucleotides LRK53_RS03865 and LRK53_RS03870 are separated by 254 nucleotides
LRK53_RS03860: LRK53_RS03860 - EAL domain-containing protein, at 902,576 to 905,536
_RS03860
LRK53_RS03865: LRK53_RS03865 - DsbA family oxidoreductase, at 905,543 to 906,196
_RS03865
LRK53_RS03870: LRK53_RS03870 - DUF3375 domain-containing protein, at 906,451 to 907,914
_RS03870
Position (kb) 905
906
907 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 904.652 kb on + strand, within LRK53_RS03860 at 904.652 kb on + strand, within LRK53_RS03860 at 904.652 kb on + strand, within LRK53_RS03860 at 904.652 kb on + strand, within LRK53_RS03860 at 904.652 kb on + strand, within LRK53_RS03860 at 904.652 kb on + strand, within LRK53_RS03860 at 904.652 kb on + strand, within LRK53_RS03860 at 904.652 kb on + strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.653 kb on - strand, within LRK53_RS03860 at 904.688 kb on - strand, within LRK53_RS03860 at 904.697 kb on + strand, within LRK53_RS03860 at 904.698 kb on - strand, within LRK53_RS03860 at 904.698 kb on - strand, within LRK53_RS03860 at 904.728 kb on - strand, within LRK53_RS03860 at 904.826 kb on + strand, within LRK53_RS03860 at 904.826 kb on + strand, within LRK53_RS03860 at 904.826 kb on + strand, within LRK53_RS03860 at 904.827 kb on - strand, within LRK53_RS03860 at 904.827 kb on - strand, within LRK53_RS03860 at 904.827 kb on - strand, within LRK53_RS03860 at 904.827 kb on - strand, within LRK53_RS03860 at 904.875 kb on - strand, within LRK53_RS03860 at 905.110 kb on + strand, within LRK53_RS03860 at 905.111 kb on - strand, within LRK53_RS03860 at 905.111 kb on - strand, within LRK53_RS03860 at 905.249 kb on + strand at 905.249 kb on + strand at 905.250 kb on - strand at 905.264 kb on + strand at 905.264 kb on + strand at 905.264 kb on + strand at 905.474 kb on + strand at 905.474 kb on + strand at 905.475 kb on - strand at 905.475 kb on - strand at 905.544 kb on + strand at 905.544 kb on + strand at 905.545 kb on - strand at 905.805 kb on + strand, within LRK53_RS03865 at 905.805 kb on + strand, within LRK53_RS03865 at 905.805 kb on + strand, within LRK53_RS03865 at 905.806 kb on - strand, within LRK53_RS03865 at 905.806 kb on - strand, within LRK53_RS03865 at 905.806 kb on - strand, within LRK53_RS03865 at 905.806 kb on - strand, within LRK53_RS03865 at 905.892 kb on + strand, within LRK53_RS03865 at 905.892 kb on + strand, within LRK53_RS03865 at 905.893 kb on - strand, within LRK53_RS03865 at 905.893 kb on - strand, within LRK53_RS03865 at 905.893 kb on - strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.994 kb on + strand, within LRK53_RS03865 at 905.995 kb on - strand, within LRK53_RS03865 at 905.995 kb on - strand, within LRK53_RS03865 at 905.995 kb on - strand, within LRK53_RS03865 at 905.995 kb on - strand, within LRK53_RS03865 at 905.995 kb on - strand, within LRK53_RS03865 at 906.271 kb on + strand at 906.271 kb on + strand at 906.272 kb on - strand at 906.300 kb on + strand at 906.300 kb on + strand at 906.300 kb on + strand at 906.300 kb on + strand at 906.300 kb on + strand at 906.301 kb on - strand at 906.301 kb on - strand at 906.301 kb on - strand at 906.301 kb on - strand at 906.310 kb on + strand at 906.311 kb on - strand at 906.311 kb on - strand at 906.311 kb on - strand at 906.311 kb on - strand at 906.360 kb on + strand at 906.360 kb on + strand at 906.360 kb on + strand at 906.360 kb on + strand at 906.361 kb on - strand at 906.361 kb on - strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.374 kb on + strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.375 kb on - strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.474 kb on + strand at 906.475 kb on - strand at 906.475 kb on - strand at 906.475 kb on - strand at 906.489 kb on - strand at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.673 kb on + strand, within LRK53_RS03870 at 906.674 kb on - strand, within LRK53_RS03870 at 906.674 kb on - strand, within LRK53_RS03870 at 906.674 kb on - strand, within LRK53_RS03870 at 906.742 kb on + strand, within LRK53_RS03870 at 906.742 kb on + strand, within LRK53_RS03870 at 906.742 kb on + strand, within LRK53_RS03870 at 906.743 kb on - strand, within LRK53_RS03870 at 906.743 kb on - strand, within LRK53_RS03870 at 906.743 kb on - strand, within LRK53_RS03870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 904,652 + LRK53_RS03860 0.70 +0.2 904,652 + LRK53_RS03860 0.70 +1.0 904,652 + LRK53_RS03860 0.70 -0.8 904,652 + LRK53_RS03860 0.70 -0.3 904,652 + LRK53_RS03860 0.70 +0.2 904,652 + LRK53_RS03860 0.70 -2.2 904,652 + LRK53_RS03860 0.70 -0.8 904,652 + LRK53_RS03860 0.70 -0.6 904,653 - LRK53_RS03860 0.70 -0.5 904,653 - LRK53_RS03860 0.70 -0.6 904,653 - LRK53_RS03860 0.70 -0.5 904,653 - LRK53_RS03860 0.70 +1.0 904,653 - LRK53_RS03860 0.70 +0.7 904,653 - LRK53_RS03860 0.70 -0.4 904,653 - LRK53_RS03860 0.70 -0.6 904,653 - LRK53_RS03860 0.70 -1.1 904,688 - LRK53_RS03860 0.71 -1.2 904,697 + LRK53_RS03860 0.72 -0.4 904,698 - LRK53_RS03860 0.72 -1.2 904,698 - LRK53_RS03860 0.72 -0.6 904,728 - LRK53_RS03860 0.73 -0.1 904,826 + LRK53_RS03860 0.76 -2.0 904,826 + LRK53_RS03860 0.76 -0.0 904,826 + LRK53_RS03860 0.76 -0.6 904,827 - LRK53_RS03860 0.76 +0.2 904,827 - LRK53_RS03860 0.76 -0.8 904,827 - LRK53_RS03860 0.76 -1.8 904,827 - LRK53_RS03860 0.76 -0.0 904,875 - LRK53_RS03860 0.78 -2.2 905,110 + LRK53_RS03860 0.86 -0.9 905,111 - LRK53_RS03860 0.86 -0.6 905,111 - LRK53_RS03860 0.86 -0.6 905,249 + -0.6 905,249 + -0.6 905,250 - -0.6 905,264 + +0.2 905,264 + -0.3 905,264 + +1.0 905,474 + +2.6 905,474 + -1.6 905,475 - +0.4 905,475 - -1.0 905,544 + -1.5 905,544 + -0.0 905,545 - -2.5 905,805 + LRK53_RS03865 0.40 -0.6 905,805 + LRK53_RS03865 0.40 -2.0 905,805 + LRK53_RS03865 0.40 -1.0 905,806 - LRK53_RS03865 0.40 -0.6 905,806 - LRK53_RS03865 0.40 +1.4 905,806 - LRK53_RS03865 0.40 -1.4 905,806 - LRK53_RS03865 0.40 -0.6 905,892 + LRK53_RS03865 0.53 -1.6 905,892 + LRK53_RS03865 0.53 -0.6 905,893 - LRK53_RS03865 0.54 -0.0 905,893 - LRK53_RS03865 0.54 -0.0 905,893 - LRK53_RS03865 0.54 -0.0 905,994 + LRK53_RS03865 0.69 -0.6 905,994 + LRK53_RS03865 0.69 +0.2 905,994 + LRK53_RS03865 0.69 +1.0 905,994 + LRK53_RS03865 0.69 -1.0 905,994 + LRK53_RS03865 0.69 -1.3 905,994 + LRK53_RS03865 0.69 -0.6 905,994 + LRK53_RS03865 0.69 -1.6 905,994 + LRK53_RS03865 0.69 -0.3 905,994 + LRK53_RS03865 0.69 -1.6 905,995 - LRK53_RS03865 0.69 -2.0 905,995 - LRK53_RS03865 0.69 -0.0 905,995 - LRK53_RS03865 0.69 -0.2 905,995 - LRK53_RS03865 0.69 -1.7 905,995 - LRK53_RS03865 0.69 +0.4 906,271 + -0.8 906,271 + -0.9 906,272 - +1.6 906,300 + -0.6 906,300 + -0.0 906,300 + -0.0 906,300 + -1.6 906,300 + +0.2 906,301 - -1.0 906,301 - -3.0 906,301 - +0.5 906,301 - -1.0 906,310 + -0.6 906,311 - -0.0 906,311 - -1.0 906,311 - -0.0 906,311 - -2.2 906,360 + -1.0 906,360 + +1.0 906,360 + +1.6 906,360 + +1.0 906,361 - -1.3 906,361 - +0.4 906,374 + -0.9 906,374 + -0.0 906,374 + -0.0 906,374 + +0.2 906,374 + -0.8 906,374 + -0.0 906,374 + -0.8 906,374 + -0.0 906,374 + -0.2 906,374 + -0.8 906,374 + -0.6 906,374 + -0.8 906,374 + -0.8 906,374 + -1.4 906,374 + -1.0 906,374 + -1.4 906,374 + -3.0 906,374 + -0.7 906,374 + -1.0 906,374 + -0.6 906,374 + +0.5 906,374 + -0.8 906,374 + -1.6 906,374 + -0.4 906,374 + -0.3 906,374 + -1.8 906,375 - -3.0 906,375 - -1.0 906,375 - -0.1 906,375 - -0.4 906,375 - -1.0 906,375 - -0.4 906,375 - -0.0 906,375 - -2.2 906,375 - -0.8 906,375 - -0.4 906,375 - +2.0 906,375 - -1.4 906,375 - -0.7 906,375 - -0.0 906,375 - -1.6 906,375 - -1.1 906,375 - +1.0 906,375 - -0.0 906,375 - -0.6 906,375 - +1.4 906,375 - -0.8 906,375 - -1.8 906,375 - +0.1 906,375 - -1.7 906,375 - +2.0 906,375 - -1.9 906,375 - -2.0 906,375 - +0.5 906,375 - -0.2 906,375 - -0.0 906,375 - -0.6 906,375 - -2.0 906,375 - +0.2 906,375 - -1.4 906,375 - -1.0 906,375 - +1.3 906,375 - -0.6 906,474 + -0.6 906,474 + -1.5 906,474 + -0.3 906,474 + -0.3 906,474 + -2.6 906,474 + -1.0 906,474 + +0.6 906,474 + -1.2 906,474 + -0.6 906,475 - -1.5 906,475 - -1.2 906,475 - -1.2 906,489 - -0.4 906,673 + LRK53_RS03870 0.15 -1.7 906,673 + LRK53_RS03870 0.15 -0.2 906,673 + LRK53_RS03870 0.15 +0.2 906,673 + LRK53_RS03870 0.15 +0.2 906,673 + LRK53_RS03870 0.15 -1.4 906,673 + LRK53_RS03870 0.15 -1.3 906,673 + LRK53_RS03870 0.15 -0.6 906,673 + LRK53_RS03870 0.15 -1.6 906,673 + LRK53_RS03870 0.15 -0.4 906,673 + LRK53_RS03870 0.15 -0.0 906,673 + LRK53_RS03870 0.15 -1.2 906,673 + LRK53_RS03870 0.15 -1.0 906,673 + LRK53_RS03870 0.15 -0.6 906,673 + LRK53_RS03870 0.15 -1.4 906,673 + LRK53_RS03870 0.15 +0.2 906,674 - LRK53_RS03870 0.15 -1.2 906,674 - LRK53_RS03870 0.15 -1.2 906,674 - LRK53_RS03870 0.15 -0.6 906,742 + LRK53_RS03870 0.20 -2.0 906,742 + LRK53_RS03870 0.20 -0.6 906,742 + LRK53_RS03870 0.20 -2.1 906,743 - LRK53_RS03870 0.20 -1.4 906,743 - LRK53_RS03870 0.20 +2.6 906,743 - LRK53_RS03870 0.20 -0.0
Or see this region's nucleotide sequence