Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03480 and LRK53_RS03485 are separated by 115 nucleotides LRK53_RS03485 and LRK53_RS03490 are separated by 18 nucleotides LRK53_RS03490 and LRK53_RS03495 are separated by 152 nucleotides
LRK53_RS03480: LRK53_RS03480 - recombination-associated protein RdgC, at 821,319 to 822,236
_RS03480
LRK53_RS03485: LRK53_RS03485 - M48 family metallopeptidase, at 822,352 to 823,122
_RS03485
LRK53_RS03490: LRK53_RS03490 - sterol desaturase family protein, at 823,141 to 823,728
_RS03490
LRK53_RS03495: LRK53_RS03495 - alpha/beta fold hydrolase, at 823,881 to 824,825
_RS03495
Position (kb) 822
823
824 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.274 kb on + strand at 822.275 kb on - strand at 822.275 kb on - strand at 822.275 kb on - strand at 822.275 kb on - strand at 822.275 kb on - strand at 822.275 kb on - strand at 822.275 kb on - strand at 822.275 kb on - strand at 822.487 kb on + strand, within LRK53_RS03485 at 822.487 kb on + strand, within LRK53_RS03485 at 822.487 kb on + strand, within LRK53_RS03485 at 822.487 kb on + strand, within LRK53_RS03485 at 822.487 kb on + strand, within LRK53_RS03485 at 822.488 kb on - strand, within LRK53_RS03485 at 822.488 kb on - strand, within LRK53_RS03485 at 822.488 kb on - strand, within LRK53_RS03485 at 822.488 kb on - strand, within LRK53_RS03485 at 822.814 kb on + strand, within LRK53_RS03485 at 822.814 kb on + strand, within LRK53_RS03485 at 822.815 kb on - strand, within LRK53_RS03485 at 822.815 kb on - strand, within LRK53_RS03485 at 822.815 kb on - strand, within LRK53_RS03485 at 822.949 kb on + strand, within LRK53_RS03485 at 822.949 kb on + strand, within LRK53_RS03485 at 822.950 kb on - strand, within LRK53_RS03485 at 822.950 kb on - strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.983 kb on + strand, within LRK53_RS03485 at 822.984 kb on - strand, within LRK53_RS03485 at 822.984 kb on - strand, within LRK53_RS03485 at 822.984 kb on - strand, within LRK53_RS03485 at 822.984 kb on - strand, within LRK53_RS03485 at 822.984 kb on - strand, within LRK53_RS03485 at 822.984 kb on - strand, within LRK53_RS03485 at 822.984 kb on - strand, within LRK53_RS03485 at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.166 kb on + strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.167 kb on - strand at 823.229 kb on + strand, within LRK53_RS03490 at 823.229 kb on + strand, within LRK53_RS03490 at 823.230 kb on - strand, within LRK53_RS03490 at 823.230 kb on - strand, within LRK53_RS03490 at 823.230 kb on - strand, within LRK53_RS03490 at 823.230 kb on - strand, within LRK53_RS03490 at 823.317 kb on - strand, within LRK53_RS03490 at 823.325 kb on + strand, within LRK53_RS03490 at 823.325 kb on + strand, within LRK53_RS03490 at 823.325 kb on + strand, within LRK53_RS03490 at 823.325 kb on + strand, within LRK53_RS03490 at 823.326 kb on - strand, within LRK53_RS03490 at 823.326 kb on - strand, within LRK53_RS03490 at 823.408 kb on + strand, within LRK53_RS03490 at 823.408 kb on + strand, within LRK53_RS03490 at 823.408 kb on + strand, within LRK53_RS03490 at 823.409 kb on - strand, within LRK53_RS03490 at 823.409 kb on - strand, within LRK53_RS03490 at 823.409 kb on - strand, within LRK53_RS03490 at 823.409 kb on - strand, within LRK53_RS03490 at 823.409 kb on - strand, within LRK53_RS03490 at 823.409 kb on - strand, within LRK53_RS03490 at 823.409 kb on - strand, within LRK53_RS03490 at 823.443 kb on - strand, within LRK53_RS03490 at 823.443 kb on - strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.526 kb on + strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.527 kb on - strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.661 kb on + strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.662 kb on - strand, within LRK53_RS03490 at 823.820 kb on + strand at 823.820 kb on + strand at 823.820 kb on + strand at 823.820 kb on + strand at 823.820 kb on + strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 823.821 kb on - strand at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.107 kb on + strand, within LRK53_RS03495 at 824.108 kb on - strand, within LRK53_RS03495 at 824.108 kb on - strand, within LRK53_RS03495 at 824.108 kb on - strand, within LRK53_RS03495
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 822,274 + -1.4 822,274 + +0.3 822,274 + -0.6 822,274 + -1.2 822,274 + +1.0 822,274 + +0.1 822,274 + -0.6 822,274 + +0.3 822,274 + -0.3 822,274 + +1.5 822,274 + -0.9 822,274 + -0.6 822,274 + -1.0 822,274 + -0.6 822,274 + -1.8 822,275 - -1.7 822,275 - -1.4 822,275 - -1.6 822,275 - +1.0 822,275 - +0.4 822,275 - -0.0 822,275 - +0.2 822,275 - -1.6 822,487 + LRK53_RS03485 0.18 +0.4 822,487 + LRK53_RS03485 0.18 -0.8 822,487 + LRK53_RS03485 0.18 -0.8 822,487 + LRK53_RS03485 0.18 -1.7 822,487 + LRK53_RS03485 0.18 -1.5 822,488 - LRK53_RS03485 0.18 -1.5 822,488 - LRK53_RS03485 0.18 -2.4 822,488 - LRK53_RS03485 0.18 -0.2 822,488 - LRK53_RS03485 0.18 -0.0 822,814 + LRK53_RS03485 0.60 -0.6 822,814 + LRK53_RS03485 0.60 -0.7 822,815 - LRK53_RS03485 0.60 +0.6 822,815 - LRK53_RS03485 0.60 -1.0 822,815 - LRK53_RS03485 0.60 +1.0 822,949 + LRK53_RS03485 0.77 -0.6 822,949 + LRK53_RS03485 0.77 -2.7 822,950 - LRK53_RS03485 0.78 +1.0 822,950 - LRK53_RS03485 0.78 -1.5 822,983 + LRK53_RS03485 0.82 -1.4 822,983 + LRK53_RS03485 0.82 +0.2 822,983 + LRK53_RS03485 0.82 +0.5 822,983 + LRK53_RS03485 0.82 -0.8 822,983 + LRK53_RS03485 0.82 -3.2 822,983 + LRK53_RS03485 0.82 -1.6 822,983 + LRK53_RS03485 0.82 -2.7 822,983 + LRK53_RS03485 0.82 -0.4 822,984 - LRK53_RS03485 0.82 +0.2 822,984 - LRK53_RS03485 0.82 -2.4 822,984 - LRK53_RS03485 0.82 -1.6 822,984 - LRK53_RS03485 0.82 +0.5 822,984 - LRK53_RS03485 0.82 +1.0 822,984 - LRK53_RS03485 0.82 -1.6 822,984 - LRK53_RS03485 0.82 +0.4 823,166 + -0.0 823,166 + -0.4 823,166 + -0.4 823,166 + +1.0 823,166 + -0.0 823,166 + -0.4 823,166 + -0.7 823,166 + -0.0 823,166 + -0.0 823,166 + -0.5 823,166 + -0.5 823,166 + -0.6 823,167 - -0.3 823,167 - -0.0 823,167 - -0.0 823,167 - +1.4 823,167 - +1.0 823,167 - +0.2 823,167 - -0.4 823,167 - +1.0 823,167 - +1.5 823,167 - +0.6 823,229 + LRK53_RS03490 0.15 +1.0 823,229 + LRK53_RS03490 0.15 -1.6 823,230 - LRK53_RS03490 0.15 -1.4 823,230 - LRK53_RS03490 0.15 -1.0 823,230 - LRK53_RS03490 0.15 -0.6 823,230 - LRK53_RS03490 0.15 -0.4 823,317 - LRK53_RS03490 0.30 -0.5 823,325 + LRK53_RS03490 0.31 -0.6 823,325 + LRK53_RS03490 0.31 -0.0 823,325 + LRK53_RS03490 0.31 -1.4 823,325 + LRK53_RS03490 0.31 +0.4 823,326 - LRK53_RS03490 0.31 -0.0 823,326 - LRK53_RS03490 0.31 -0.7 823,408 + LRK53_RS03490 0.45 -1.6 823,408 + LRK53_RS03490 0.45 -0.7 823,408 + LRK53_RS03490 0.45 -1.6 823,409 - LRK53_RS03490 0.46 +1.4 823,409 - LRK53_RS03490 0.46 -2.0 823,409 - LRK53_RS03490 0.46 -0.2 823,409 - LRK53_RS03490 0.46 -0.0 823,409 - LRK53_RS03490 0.46 -1.4 823,409 - LRK53_RS03490 0.46 +0.2 823,409 - LRK53_RS03490 0.46 -1.0 823,443 - LRK53_RS03490 0.51 -1.0 823,443 - LRK53_RS03490 0.51 -1.0 823,526 + LRK53_RS03490 0.65 +0.4 823,526 + LRK53_RS03490 0.65 +1.0 823,526 + LRK53_RS03490 0.65 +1.0 823,526 + LRK53_RS03490 0.65 -1.7 823,526 + LRK53_RS03490 0.65 -0.5 823,526 + LRK53_RS03490 0.65 +0.2 823,526 + LRK53_RS03490 0.65 -0.6 823,526 + LRK53_RS03490 0.65 -2.4 823,527 - LRK53_RS03490 0.66 +0.2 823,527 - LRK53_RS03490 0.66 +0.3 823,527 - LRK53_RS03490 0.66 +2.0 823,527 - LRK53_RS03490 0.66 -0.6 823,527 - LRK53_RS03490 0.66 -1.0 823,527 - LRK53_RS03490 0.66 -0.2 823,527 - LRK53_RS03490 0.66 +1.0 823,527 - LRK53_RS03490 0.66 -2.4 823,527 - LRK53_RS03490 0.66 -0.0 823,527 - LRK53_RS03490 0.66 -1.4 823,527 - LRK53_RS03490 0.66 -0.0 823,527 - LRK53_RS03490 0.66 -2.7 823,661 + LRK53_RS03490 0.88 -1.4 823,661 + LRK53_RS03490 0.88 +1.0 823,661 + LRK53_RS03490 0.88 +0.2 823,661 + LRK53_RS03490 0.88 +0.6 823,661 + LRK53_RS03490 0.88 -0.7 823,661 + LRK53_RS03490 0.88 -0.6 823,661 + LRK53_RS03490 0.88 -2.7 823,661 + LRK53_RS03490 0.88 +0.2 823,661 + LRK53_RS03490 0.88 +1.0 823,662 - LRK53_RS03490 0.89 -2.0 823,662 - LRK53_RS03490 0.89 +1.0 823,662 - LRK53_RS03490 0.89 -0.6 823,662 - LRK53_RS03490 0.89 -1.0 823,662 - LRK53_RS03490 0.89 +1.7 823,662 - LRK53_RS03490 0.89 -0.0 823,662 - LRK53_RS03490 0.89 -0.0 823,662 - LRK53_RS03490 0.89 +0.3 823,662 - LRK53_RS03490 0.89 -1.2 823,662 - LRK53_RS03490 0.89 +0.4 823,662 - LRK53_RS03490 0.89 -1.4 823,662 - LRK53_RS03490 0.89 -1.7 823,662 - LRK53_RS03490 0.89 -1.0 823,662 - LRK53_RS03490 0.89 +1.1 823,662 - LRK53_RS03490 0.89 -0.6 823,662 - LRK53_RS03490 0.89 -0.0 823,662 - LRK53_RS03490 0.89 -2.0 823,662 - LRK53_RS03490 0.89 +1.0 823,820 + -1.0 823,820 + +1.3 823,820 + +1.5 823,820 + -3.1 823,820 + -1.4 823,821 - -0.6 823,821 - -0.2 823,821 - -0.6 823,821 - -0.3 823,821 - -1.0 823,821 - -0.2 823,821 - -1.4 823,821 - -0.6 823,821 - -1.2 823,821 - +3.5 823,821 - +0.2 823,821 - -0.4 823,821 - -0.0 823,821 - +0.4 823,821 - +0.2 824,107 + LRK53_RS03495 0.24 -1.4 824,107 + LRK53_RS03495 0.24 -0.8 824,107 + LRK53_RS03495 0.24 -0.6 824,107 + LRK53_RS03495 0.24 -0.8 824,107 + LRK53_RS03495 0.24 -1.0 824,107 + LRK53_RS03495 0.24 +0.3 824,107 + LRK53_RS03495 0.24 -1.8 824,107 + LRK53_RS03495 0.24 -0.8 824,107 + LRK53_RS03495 0.24 -0.4 824,108 - LRK53_RS03495 0.24 -1.8 824,108 - LRK53_RS03495 0.24 -1.0 824,108 - LRK53_RS03495 0.24 -0.2
Or see this region's nucleotide sequence