Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS12635 and LRK53_RS12640 are separated by 476 nucleotides LRK53_RS12640 and LRK53_RS12645 are separated by 163 nucleotides LRK53_RS12645 and LRK53_RS12650 are separated by 25 nucleotides
LRK53_RS12635: LRK53_RS12635 - folate-binding protein YgfZ, at 2,797,112 to 2,797,915
_RS12635
LRK53_RS12640: LRK53_RS12640 - cell wall hydrolase, at 2,798,392 to 2,798,826
_RS12640
LRK53_RS12645: LRK53_RS12645 - glycine zipper 2TM domain-containing protein, at 2,798,990 to 2,799,490
_RS12645
LRK53_RS12650: LRK53_RS12650 - glycine zipper 2TM domain-containing protein, at 2,799,516 to 2,800,097
_RS12650
Position (kb)
2798
2799 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.478 kb on + strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.479 kb on - strand, within LRK53_RS12635 at 2797.817 kb on + strand, within LRK53_RS12635 at 2797.818 kb on + strand, within LRK53_RS12635 at 2797.818 kb on + strand, within LRK53_RS12635 at 2797.819 kb on - strand, within LRK53_RS12635 at 2797.819 kb on - strand, within LRK53_RS12635 at 2797.819 kb on - strand, within LRK53_RS12635 at 2797.896 kb on + strand at 2797.948 kb on + strand at 2797.948 kb on + strand at 2797.949 kb on - strand at 2797.949 kb on - strand at 2798.025 kb on + strand at 2798.026 kb on - strand at 2798.074 kb on - strand at 2798.074 kb on - strand at 2798.165 kb on - strand at 2798.370 kb on - strand at 2798.370 kb on - strand at 2798.370 kb on - strand at 2798.370 kb on - strand at 2798.370 kb on - strand at 2798.370 kb on - strand at 2798.473 kb on + strand, within LRK53_RS12640 at 2798.473 kb on + strand, within LRK53_RS12640 at 2798.473 kb on + strand, within LRK53_RS12640 at 2798.473 kb on + strand, within LRK53_RS12640 at 2798.474 kb on - strand, within LRK53_RS12640 at 2798.474 kb on - strand, within LRK53_RS12640 at 2798.474 kb on - strand, within LRK53_RS12640 at 2798.717 kb on + strand, within LRK53_RS12640 at 2798.717 kb on + strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.718 kb on - strand, within LRK53_RS12640 at 2798.812 kb on + strand at 2798.813 kb on - strand at 2798.813 kb on - strand at 2798.987 kb on + strand at 2798.987 kb on + strand at 2798.988 kb on - strand at 2798.988 kb on - strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.000 kb on + strand at 2799.027 kb on + strand at 2799.027 kb on + strand at 2799.027 kb on + strand at 2799.027 kb on + strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.028 kb on - strand at 2799.075 kb on + strand, within LRK53_RS12645 at 2799.076 kb on - strand, within LRK53_RS12645 at 2799.076 kb on - strand, within LRK53_RS12645 at 2799.294 kb on + strand, within LRK53_RS12645 at 2799.294 kb on + strand, within LRK53_RS12645 at 2799.294 kb on + strand, within LRK53_RS12645 at 2799.379 kb on - strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.390 kb on + strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.391 kb on - strand, within LRK53_RS12645 at 2799.408 kb on + strand, within LRK53_RS12645 at 2799.409 kb on - strand, within LRK53_RS12645 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.610 kb on + strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.611 kb on - strand, within LRK53_RS12650 at 2799.617 kb on - strand, within LRK53_RS12650 at 2799.628 kb on + strand, within LRK53_RS12650 at 2799.628 kb on + strand, within LRK53_RS12650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 2,797,478 + LRK53_RS12635 0.46 +2.1 2,797,478 + LRK53_RS12635 0.46 +1.1 2,797,478 + LRK53_RS12635 0.46 +0.1 2,797,478 + LRK53_RS12635 0.46 -0.4 2,797,478 + LRK53_RS12635 0.46 -2.2 2,797,478 + LRK53_RS12635 0.46 -0.9 2,797,478 + LRK53_RS12635 0.46 -2.4 2,797,478 + LRK53_RS12635 0.46 -0.5 2,797,478 + LRK53_RS12635 0.46 -0.2 2,797,478 + LRK53_RS12635 0.46 +0.3 2,797,478 + LRK53_RS12635 0.46 +2.1 2,797,478 + LRK53_RS12635 0.46 -1.5 2,797,479 - LRK53_RS12635 0.46 -1.9 2,797,479 - LRK53_RS12635 0.46 -1.2 2,797,479 - LRK53_RS12635 0.46 +1.1 2,797,479 - LRK53_RS12635 0.46 +1.1 2,797,479 - LRK53_RS12635 0.46 -0.5 2,797,479 - LRK53_RS12635 0.46 -1.5 2,797,479 - LRK53_RS12635 0.46 -1.0 2,797,479 - LRK53_RS12635 0.46 +1.7 2,797,479 - LRK53_RS12635 0.46 -1.0 2,797,479 - LRK53_RS12635 0.46 -1.1 2,797,479 - LRK53_RS12635 0.46 -0.9 2,797,479 - LRK53_RS12635 0.46 -0.4 2,797,817 + LRK53_RS12635 0.88 -0.4 2,797,818 + LRK53_RS12635 0.88 +1.5 2,797,818 + LRK53_RS12635 0.88 -2.4 2,797,819 - LRK53_RS12635 0.88 -1.2 2,797,819 - LRK53_RS12635 0.88 -0.1 2,797,819 - LRK53_RS12635 0.88 +0.1 2,797,896 + -0.3 2,797,948 + -0.5 2,797,948 + -1.5 2,797,949 - +1.1 2,797,949 - -0.2 2,798,025 + -0.5 2,798,026 - +0.1 2,798,074 - +0.1 2,798,074 - -0.7 2,798,165 - +0.4 2,798,370 - +0.0 2,798,370 - +0.1 2,798,370 - +0.1 2,798,370 - -1.5 2,798,370 - -0.5 2,798,370 - -0.5 2,798,473 + LRK53_RS12640 0.19 -1.2 2,798,473 + LRK53_RS12640 0.19 -0.3 2,798,473 + LRK53_RS12640 0.19 +0.3 2,798,473 + LRK53_RS12640 0.19 +1.1 2,798,474 - LRK53_RS12640 0.19 -0.8 2,798,474 - LRK53_RS12640 0.19 +0.8 2,798,474 - LRK53_RS12640 0.19 -1.0 2,798,717 + LRK53_RS12640 0.75 +0.1 2,798,717 + LRK53_RS12640 0.75 +1.1 2,798,718 - LRK53_RS12640 0.75 -0.5 2,798,718 - LRK53_RS12640 0.75 +1.1 2,798,718 - LRK53_RS12640 0.75 -1.6 2,798,718 - LRK53_RS12640 0.75 +2.1 2,798,812 + +0.1 2,798,813 - +0.6 2,798,813 - -1.2 2,798,987 + -1.2 2,798,987 + +1.1 2,798,988 - +0.4 2,798,988 - -1.7 2,799,000 + -0.1 2,799,000 + -0.5 2,799,000 + -1.4 2,799,000 + -1.0 2,799,000 + -0.9 2,799,000 + +0.1 2,799,000 + +0.7 2,799,027 + -0.4 2,799,027 + +1.1 2,799,027 + +0.1 2,799,027 + +1.1 2,799,028 - -1.7 2,799,028 - +0.5 2,799,028 - +0.1 2,799,028 - -0.5 2,799,028 - -0.5 2,799,075 + LRK53_RS12645 0.17 +1.1 2,799,076 - LRK53_RS12645 0.17 -1.1 2,799,076 - LRK53_RS12645 0.17 -0.1 2,799,294 + LRK53_RS12645 0.61 -1.8 2,799,294 + LRK53_RS12645 0.61 -0.5 2,799,294 + LRK53_RS12645 0.61 -1.6 2,799,379 - LRK53_RS12645 0.78 +0.4 2,799,390 + LRK53_RS12645 0.80 +0.5 2,799,390 + LRK53_RS12645 0.80 +2.4 2,799,390 + LRK53_RS12645 0.80 -0.8 2,799,390 + LRK53_RS12645 0.80 +0.4 2,799,390 + LRK53_RS12645 0.80 +0.7 2,799,390 + LRK53_RS12645 0.80 -0.9 2,799,391 - LRK53_RS12645 0.80 +0.1 2,799,391 - LRK53_RS12645 0.80 -0.1 2,799,391 - LRK53_RS12645 0.80 +0.1 2,799,391 - LRK53_RS12645 0.80 +0.7 2,799,408 + LRK53_RS12645 0.83 -0.9 2,799,409 - LRK53_RS12645 0.84 -0.8 2,799,610 + LRK53_RS12650 0.16 -0.0 2,799,610 + LRK53_RS12650 0.16 +1.1 2,799,610 + LRK53_RS12650 0.16 -2.4 2,799,610 + LRK53_RS12650 0.16 +2.1 2,799,610 + LRK53_RS12650 0.16 +0.1 2,799,610 + LRK53_RS12650 0.16 -1.7 2,799,610 + LRK53_RS12650 0.16 -0.5 2,799,610 + LRK53_RS12650 0.16 -0.9 2,799,610 + LRK53_RS12650 0.16 -0.2 2,799,610 + LRK53_RS12650 0.16 -1.1 2,799,610 + LRK53_RS12650 0.16 +0.4 2,799,610 + LRK53_RS12650 0.16 -1.2 2,799,611 - LRK53_RS12650 0.16 -0.9 2,799,611 - LRK53_RS12650 0.16 -0.7 2,799,611 - LRK53_RS12650 0.16 -1.4 2,799,611 - LRK53_RS12650 0.16 -0.5 2,799,611 - LRK53_RS12650 0.16 +0.1 2,799,611 - LRK53_RS12650 0.16 -0.7 2,799,611 - LRK53_RS12650 0.16 +0.7 2,799,611 - LRK53_RS12650 0.16 -0.5 2,799,611 - LRK53_RS12650 0.16 +1.1 2,799,611 - LRK53_RS12650 0.16 -0.7 2,799,611 - LRK53_RS12650 0.16 +1.1 2,799,611 - LRK53_RS12650 0.16 +0.1 2,799,611 - LRK53_RS12650 0.16 +0.1 2,799,611 - LRK53_RS12650 0.16 +0.7 2,799,617 - LRK53_RS12650 0.17 -0.9 2,799,628 + LRK53_RS12650 0.19 +0.1 2,799,628 + LRK53_RS12650 0.19 -0.5
Or see this region's nucleotide sequence