Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11845 and LRK53_RS11850 are separated by 112 nucleotides LRK53_RS11850 and LRK53_RS11855 are separated by 151 nucleotides
LRK53_RS11845: LRK53_RS11845 - phospholipase, at 2,638,693 to 2,640,132
_RS11845
LRK53_RS11850: LRK53_RS11850 - hypothetical protein, at 2,640,245 to 2,640,742
_RS11850
LRK53_RS11855: LRK53_RS11855 - glycosyltransferase, at 2,640,894 to 2,644,316
_RS11855
Position (kb)
2640
2641 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2639.260 kb on + strand, within LRK53_RS11845 at 2639.261 kb on - strand, within LRK53_RS11845 at 2639.261 kb on - strand, within LRK53_RS11845 at 2639.261 kb on - strand, within LRK53_RS11845 at 2639.261 kb on - strand, within LRK53_RS11845 at 2639.261 kb on - strand, within LRK53_RS11845 at 2639.261 kb on - strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.278 kb on + strand, within LRK53_RS11845 at 2639.279 kb on - strand, within LRK53_RS11845 at 2639.279 kb on - strand, within LRK53_RS11845 at 2639.279 kb on - strand, within LRK53_RS11845 at 2639.279 kb on - strand, within LRK53_RS11845 at 2639.279 kb on - strand, within LRK53_RS11845 at 2639.464 kb on + strand, within LRK53_RS11845 at 2639.465 kb on - strand, within LRK53_RS11845 at 2639.465 kb on - strand, within LRK53_RS11845 at 2639.465 kb on - strand, within LRK53_RS11845 at 2639.542 kb on + strand, within LRK53_RS11845 at 2639.542 kb on + strand, within LRK53_RS11845 at 2639.911 kb on + strand, within LRK53_RS11845 at 2639.912 kb on - strand, within LRK53_RS11845 at 2639.999 kb on + strand at 2639.999 kb on + strand at 2640.000 kb on - strand at 2640.000 kb on - strand at 2640.000 kb on - strand at 2640.171 kb on + strand at 2640.171 kb on + strand at 2640.171 kb on + strand at 2640.171 kb on + strand at 2640.171 kb on + strand at 2640.171 kb on + strand at 2640.171 kb on + strand at 2640.172 kb on - strand at 2640.172 kb on - strand at 2640.172 kb on - strand at 2640.172 kb on - strand at 2640.172 kb on - strand at 2640.177 kb on - strand at 2640.293 kb on + strand at 2640.293 kb on + strand at 2640.293 kb on + strand at 2640.293 kb on + strand at 2640.294 kb on - strand at 2640.294 kb on - strand at 2640.294 kb on - strand at 2640.294 kb on - strand at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.341 kb on + strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.342 kb on - strand, within LRK53_RS11850 at 2640.410 kb on + strand, within LRK53_RS11850 at 2640.411 kb on - strand, within LRK53_RS11850 at 2640.551 kb on + strand, within LRK53_RS11850 at 2640.551 kb on + strand, within LRK53_RS11850 at 2640.551 kb on + strand, within LRK53_RS11850 at 2640.551 kb on + strand, within LRK53_RS11850 at 2640.551 kb on + strand, within LRK53_RS11850 at 2640.551 kb on + strand, within LRK53_RS11850 at 2640.551 kb on + strand, within LRK53_RS11850 at 2640.552 kb on - strand, within LRK53_RS11850 at 2640.552 kb on - strand, within LRK53_RS11850 at 2640.552 kb on - strand, within LRK53_RS11850 at 2640.552 kb on - strand, within LRK53_RS11850 at 2640.557 kb on + strand, within LRK53_RS11850 at 2640.557 kb on + strand, within LRK53_RS11850 at 2640.557 kb on + strand, within LRK53_RS11850 at 2640.558 kb on - strand, within LRK53_RS11850 at 2640.558 kb on - strand, within LRK53_RS11850 at 2640.558 kb on - strand, within LRK53_RS11850 at 2640.558 kb on - strand, within LRK53_RS11850 at 2640.558 kb on - strand, within LRK53_RS11850 at 2640.558 kb on - strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.672 kb on + strand, within LRK53_RS11850 at 2640.673 kb on - strand, within LRK53_RS11850 at 2640.673 kb on - strand, within LRK53_RS11850 at 2640.673 kb on - strand, within LRK53_RS11850 at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.728 kb on + strand at 2640.729 kb on - strand at 2640.729 kb on - strand at 2640.729 kb on - strand at 2640.729 kb on - strand at 2640.729 kb on - strand at 2640.729 kb on - strand at 2640.740 kb on + strand at 2640.740 kb on + strand at 2640.740 kb on + strand at 2640.740 kb on + strand at 2640.741 kb on - strand at 2640.741 kb on - strand at 2640.805 kb on + strand at 2640.805 kb on + strand at 2640.805 kb on + strand at 2640.806 kb on - strand at 2640.806 kb on - strand at 2640.806 kb on - strand at 2640.806 kb on - strand at 2640.806 kb on - strand at 2640.830 kb on + strand at 2640.830 kb on + strand at 2640.831 kb on - strand at 2640.831 kb on - strand at 2640.831 kb on - strand at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.254 kb on + strand, within LRK53_RS11855 at 2641.255 kb on - strand, within LRK53_RS11855 at 2641.255 kb on - strand, within LRK53_RS11855 at 2641.255 kb on - strand, within LRK53_RS11855 at 2641.279 kb on - strand, within LRK53_RS11855 at 2641.364 kb on + strand, within LRK53_RS11855 at 2641.364 kb on + strand, within LRK53_RS11855 at 2641.364 kb on + strand, within LRK53_RS11855 at 2641.364 kb on + strand, within LRK53_RS11855 at 2641.364 kb on + strand, within LRK53_RS11855 at 2641.364 kb on + strand, within LRK53_RS11855 at 2641.365 kb on - strand, within LRK53_RS11855 at 2641.365 kb on - strand, within LRK53_RS11855 at 2641.365 kb on - strand, within LRK53_RS11855 at 2641.365 kb on - strand, within LRK53_RS11855 at 2641.365 kb on - strand, within LRK53_RS11855 at 2641.365 kb on - strand, within LRK53_RS11855 at 2641.698 kb on + strand, within LRK53_RS11855 at 2641.699 kb on - strand, within LRK53_RS11855 at 2641.699 kb on - strand, within LRK53_RS11855
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 2,639,260 + LRK53_RS11845 0.39 -0.2 2,639,261 - LRK53_RS11845 0.39 +2.1 2,639,261 - LRK53_RS11845 0.39 +0.1 2,639,261 - LRK53_RS11845 0.39 +1.7 2,639,261 - LRK53_RS11845 0.39 +2.1 2,639,261 - LRK53_RS11845 0.39 -0.4 2,639,261 - LRK53_RS11845 0.39 +1.1 2,639,278 + LRK53_RS11845 0.41 +1.1 2,639,278 + LRK53_RS11845 0.41 +1.1 2,639,278 + LRK53_RS11845 0.41 -1.6 2,639,278 + LRK53_RS11845 0.41 -0.9 2,639,278 + LRK53_RS11845 0.41 +0.1 2,639,278 + LRK53_RS11845 0.41 +0.1 2,639,278 + LRK53_RS11845 0.41 +0.1 2,639,278 + LRK53_RS11845 0.41 -0.9 2,639,278 + LRK53_RS11845 0.41 -0.5 2,639,278 + LRK53_RS11845 0.41 +1.1 2,639,279 - LRK53_RS11845 0.41 +1.1 2,639,279 - LRK53_RS11845 0.41 +1.1 2,639,279 - LRK53_RS11845 0.41 +0.1 2,639,279 - LRK53_RS11845 0.41 -1.0 2,639,279 - LRK53_RS11845 0.41 -0.8 2,639,464 + LRK53_RS11845 0.54 -1.8 2,639,465 - LRK53_RS11845 0.54 -1.5 2,639,465 - LRK53_RS11845 0.54 +0.5 2,639,465 - LRK53_RS11845 0.54 -1.7 2,639,542 + LRK53_RS11845 0.59 -0.6 2,639,542 + LRK53_RS11845 0.59 -0.0 2,639,911 + LRK53_RS11845 0.85 -3.4 2,639,912 - LRK53_RS11845 0.85 -1.5 2,639,999 + +0.1 2,639,999 + -0.1 2,640,000 - +1.1 2,640,000 - -0.9 2,640,000 - +0.1 2,640,171 + -0.3 2,640,171 + +2.1 2,640,171 + -0.5 2,640,171 + -0.2 2,640,171 + -0.7 2,640,171 + +0.1 2,640,171 + -2.6 2,640,172 - +0.4 2,640,172 - -0.5 2,640,172 - -1.0 2,640,172 - -0.9 2,640,172 - +0.1 2,640,177 - -1.3 2,640,293 + -2.3 2,640,293 + -2.0 2,640,293 + -0.8 2,640,293 + +0.5 2,640,294 - +0.1 2,640,294 - -1.7 2,640,294 - -2.4 2,640,294 - -0.7 2,640,341 + LRK53_RS11850 0.19 -0.9 2,640,341 + LRK53_RS11850 0.19 -1.5 2,640,341 + LRK53_RS11850 0.19 -1.3 2,640,341 + LRK53_RS11850 0.19 -0.9 2,640,341 + LRK53_RS11850 0.19 -0.5 2,640,341 + LRK53_RS11850 0.19 -0.7 2,640,341 + LRK53_RS11850 0.19 -0.5 2,640,341 + LRK53_RS11850 0.19 +1.1 2,640,341 + LRK53_RS11850 0.19 +0.2 2,640,341 + LRK53_RS11850 0.19 -0.5 2,640,341 + LRK53_RS11850 0.19 +1.1 2,640,341 + LRK53_RS11850 0.19 -0.9 2,640,341 + LRK53_RS11850 0.19 -0.6 2,640,341 + LRK53_RS11850 0.19 -1.0 2,640,341 + LRK53_RS11850 0.19 -0.7 2,640,342 - LRK53_RS11850 0.19 -0.8 2,640,342 - LRK53_RS11850 0.19 -0.5 2,640,342 - LRK53_RS11850 0.19 -0.6 2,640,342 - LRK53_RS11850 0.19 -0.9 2,640,342 - LRK53_RS11850 0.19 -0.9 2,640,342 - LRK53_RS11850 0.19 -0.5 2,640,342 - LRK53_RS11850 0.19 +1.1 2,640,342 - LRK53_RS11850 0.19 -0.3 2,640,342 - LRK53_RS11850 0.19 +0.7 2,640,410 + LRK53_RS11850 0.33 -1.1 2,640,411 - LRK53_RS11850 0.33 -0.7 2,640,551 + LRK53_RS11850 0.61 +1.1 2,640,551 + LRK53_RS11850 0.61 +1.1 2,640,551 + LRK53_RS11850 0.61 -0.9 2,640,551 + LRK53_RS11850 0.61 +1.1 2,640,551 + LRK53_RS11850 0.61 -1.2 2,640,551 + LRK53_RS11850 0.61 -1.0 2,640,551 + LRK53_RS11850 0.61 -0.5 2,640,552 - LRK53_RS11850 0.62 +1.1 2,640,552 - LRK53_RS11850 0.62 -1.2 2,640,552 - LRK53_RS11850 0.62 +0.7 2,640,552 - LRK53_RS11850 0.62 -0.5 2,640,557 + LRK53_RS11850 0.63 +0.5 2,640,557 + LRK53_RS11850 0.63 +0.1 2,640,557 + LRK53_RS11850 0.63 +0.5 2,640,558 - LRK53_RS11850 0.63 -0.2 2,640,558 - LRK53_RS11850 0.63 -0.6 2,640,558 - LRK53_RS11850 0.63 -1.0 2,640,558 - LRK53_RS11850 0.63 -1.0 2,640,558 - LRK53_RS11850 0.63 -0.5 2,640,558 - LRK53_RS11850 0.63 +0.1 2,640,672 + LRK53_RS11850 0.86 -0.3 2,640,672 + LRK53_RS11850 0.86 +1.1 2,640,672 + LRK53_RS11850 0.86 -0.9 2,640,672 + LRK53_RS11850 0.86 -0.5 2,640,672 + LRK53_RS11850 0.86 -1.2 2,640,672 + LRK53_RS11850 0.86 -0.1 2,640,672 + LRK53_RS11850 0.86 -1.2 2,640,672 + LRK53_RS11850 0.86 +0.2 2,640,672 + LRK53_RS11850 0.86 +0.1 2,640,673 - LRK53_RS11850 0.86 -1.3 2,640,673 - LRK53_RS11850 0.86 +1.1 2,640,673 - LRK53_RS11850 0.86 +0.4 2,640,728 + -2.0 2,640,728 + -0.5 2,640,728 + +1.1 2,640,728 + -0.5 2,640,728 + +1.1 2,640,728 + +0.1 2,640,728 + +0.1 2,640,728 + -1.7 2,640,728 + -1.1 2,640,729 - +0.1 2,640,729 - -0.6 2,640,729 - +1.1 2,640,729 - +0.0 2,640,729 - -1.5 2,640,729 - -0.5 2,640,740 + -0.9 2,640,740 + +0.1 2,640,740 + -0.9 2,640,740 + +2.1 2,640,741 - +1.1 2,640,741 - -0.9 2,640,805 + -0.7 2,640,805 + +0.3 2,640,805 + -0.5 2,640,806 - -0.9 2,640,806 - -0.9 2,640,806 - +0.1 2,640,806 - -1.2 2,640,806 - +1.1 2,640,830 + -1.3 2,640,830 + -3.5 2,640,831 - -1.7 2,640,831 - -0.7 2,640,831 - +0.1 2,641,254 + LRK53_RS11855 0.11 -0.9 2,641,254 + LRK53_RS11855 0.11 +1.1 2,641,254 + LRK53_RS11855 0.11 -1.2 2,641,254 + LRK53_RS11855 0.11 -0.9 2,641,254 + LRK53_RS11855 0.11 -2.0 2,641,254 + LRK53_RS11855 0.11 -1.6 2,641,254 + LRK53_RS11855 0.11 -0.7 2,641,254 + LRK53_RS11855 0.11 -0.8 2,641,255 - LRK53_RS11855 0.11 +1.1 2,641,255 - LRK53_RS11855 0.11 +1.1 2,641,255 - LRK53_RS11855 0.11 -2.3 2,641,279 - LRK53_RS11855 0.11 +0.1 2,641,364 + LRK53_RS11855 0.14 -0.3 2,641,364 + LRK53_RS11855 0.14 +1.1 2,641,364 + LRK53_RS11855 0.14 -1.2 2,641,364 + LRK53_RS11855 0.14 +0.1 2,641,364 + LRK53_RS11855 0.14 -0.5 2,641,364 + LRK53_RS11855 0.14 +0.1 2,641,365 - LRK53_RS11855 0.14 +0.1 2,641,365 - LRK53_RS11855 0.14 +1.1 2,641,365 - LRK53_RS11855 0.14 -0.6 2,641,365 - LRK53_RS11855 0.14 -0.0 2,641,365 - LRK53_RS11855 0.14 +1.1 2,641,365 - LRK53_RS11855 0.14 -0.0 2,641,698 + LRK53_RS11855 0.23 -1.2 2,641,699 - LRK53_RS11855 0.24 -0.3 2,641,699 - LRK53_RS11855 0.24 -3.3
Or see this region's nucleotide sequence