Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04570 and LRK53_RS04575 are separated by 17 nucleotides LRK53_RS04575 and LRK53_RS04580 overlap by 4 nucleotides LRK53_RS04580 and LRK53_RS04585 are separated by 130 nucleotides
LRK53_RS04570: LRK53_RS04570 - DUF4097 family beta strand repeat-containing protein, at 1,063,349 to 1,064,251
_RS04570
LRK53_RS04575: LRK53_RS04575 - hypothetical protein, at 1,064,269 to 1,064,727
_RS04575
LRK53_RS04580: LRK53_RS04580 - sigma-70 family RNA polymerase sigma factor, at 1,064,724 to 1,065,269
_RS04580
LRK53_RS04585: LRK53_RS04585 - phosphoenolpyruvate carboxykinase (GTP), at 1,065,400 to 1,067,184
_RS04585
Position (kb)
1064
1065
1066 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1065.031 kb on + strand, within LRK53_RS04580 at 1065.185 kb on - strand, within LRK53_RS04580 at 1065.515 kb on + strand at 1065.515 kb on + strand at 1065.515 kb on + strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.824 kb on + strand, within LRK53_RS04585 at 1065.824 kb on + strand, within LRK53_RS04585 at 1065.825 kb on - strand, within LRK53_RS04585 at 1065.825 kb on - strand, within LRK53_RS04585 at 1065.825 kb on - strand, within LRK53_RS04585 at 1065.825 kb on - strand, within LRK53_RS04585 at 1065.825 kb on - strand, within LRK53_RS04585 at 1065.825 kb on - strand, within LRK53_RS04585 at 1065.833 kb on + strand, within LRK53_RS04585 at 1065.833 kb on + strand, within LRK53_RS04585 at 1065.834 kb on - strand, within LRK53_RS04585 at 1065.839 kb on + strand, within LRK53_RS04585 at 1065.840 kb on - strand, within LRK53_RS04585 at 1066.127 kb on + strand, within LRK53_RS04585 at 1066.127 kb on + strand, within LRK53_RS04585 at 1066.128 kb on - strand, within LRK53_RS04585 at 1066.128 kb on - strand, within LRK53_RS04585
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 1,065,031 + LRK53_RS04580 0.56 -1.2 1,065,185 - LRK53_RS04580 0.84 -1.9 1,065,515 + -1.3 1,065,515 + -0.4 1,065,515 + -0.3 1,065,516 - -1.9 1,065,516 - -0.9 1,065,516 - -0.6 1,065,516 - +0.1 1,065,516 - +1.7 1,065,516 - -2.3 1,065,516 - -0.7 1,065,824 + LRK53_RS04585 0.24 -1.5 1,065,824 + LRK53_RS04585 0.24 -0.5 1,065,825 - LRK53_RS04585 0.24 -0.5 1,065,825 - LRK53_RS04585 0.24 -1.2 1,065,825 - LRK53_RS04585 0.24 -0.9 1,065,825 - LRK53_RS04585 0.24 +0.4 1,065,825 - LRK53_RS04585 0.24 -1.4 1,065,825 - LRK53_RS04585 0.24 -1.5 1,065,833 + LRK53_RS04585 0.24 +0.1 1,065,833 + LRK53_RS04585 0.24 -1.4 1,065,834 - LRK53_RS04585 0.24 +1.1 1,065,839 + LRK53_RS04585 0.25 -0.5 1,065,840 - LRK53_RS04585 0.25 +1.1 1,066,127 + LRK53_RS04585 0.41 -1.5 1,066,127 + LRK53_RS04585 0.41 +3.1 1,066,128 - LRK53_RS04585 0.41 -0.9 1,066,128 - LRK53_RS04585 0.41 -3.0
Or see this region's nucleotide sequence