Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS03275 and LRK53_RS03280 are separated by 12 nucleotides LRK53_RS03280 and LRK53_RS03285 are separated by 244 nucleotides
LRK53_RS03275: LRK53_RS03275 - amino acid permease, at 772,021 to 773,532
_RS03275
LRK53_RS03280: LRK53_RS03280 - GGDEF domain-containing protein, at 773,545 to 774,627
_RS03280
LRK53_RS03285: LRK53_RS03285 - M61 family metallopeptidase, at 774,872 to 776,770
_RS03285
Position (kb) 773
774
775 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 772.708 kb on + strand, within LRK53_RS03275 at 772.708 kb on + strand, within LRK53_RS03275 at 772.708 kb on + strand, within LRK53_RS03275 at 772.708 kb on + strand, within LRK53_RS03275 at 772.708 kb on + strand, within LRK53_RS03275 at 772.708 kb on + strand, within LRK53_RS03275 at 772.709 kb on - strand, within LRK53_RS03275 at 772.709 kb on - strand, within LRK53_RS03275 at 772.709 kb on - strand, within LRK53_RS03275 at 772.709 kb on - strand, within LRK53_RS03275 at 772.837 kb on + strand, within LRK53_RS03275 at 772.933 kb on + strand, within LRK53_RS03275 at 772.933 kb on + strand, within LRK53_RS03275 at 772.933 kb on + strand, within LRK53_RS03275 at 772.934 kb on - strand, within LRK53_RS03275 at 772.934 kb on - strand, within LRK53_RS03275 at 772.934 kb on - strand, within LRK53_RS03275 at 772.934 kb on - strand, within LRK53_RS03275 at 772.972 kb on + strand, within LRK53_RS03275 at 772.973 kb on - strand, within LRK53_RS03275 at 772.973 kb on - strand, within LRK53_RS03275 at 772.973 kb on - strand, within LRK53_RS03275 at 773.416 kb on + strand at 773.437 kb on + strand at 773.438 kb on - strand at 773.479 kb on + strand at 773.479 kb on + strand at 773.479 kb on + strand at 773.479 kb on + strand at 773.479 kb on + strand at 773.479 kb on + strand at 773.479 kb on + strand at 773.480 kb on - strand at 773.480 kb on - strand at 773.480 kb on - strand at 773.480 kb on - strand at 773.480 kb on - strand at 773.480 kb on - strand at 773.480 kb on - strand at 773.489 kb on - strand at 773.489 kb on - strand at 773.885 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.131 kb on + strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.132 kb on - strand, within LRK53_RS03280 at 774.140 kb on + strand, within LRK53_RS03280 at 774.140 kb on + strand, within LRK53_RS03280 at 774.140 kb on + strand, within LRK53_RS03280 at 774.141 kb on - strand, within LRK53_RS03280 at 774.149 kb on - strand, within LRK53_RS03280 at 774.149 kb on - strand, within LRK53_RS03280 at 774.149 kb on - strand, within LRK53_RS03280 at 774.149 kb on - strand, within LRK53_RS03280 at 774.501 kb on - strand, within LRK53_RS03280 at 774.501 kb on - strand, within LRK53_RS03280 at 774.501 kb on - strand, within LRK53_RS03280 at 774.501 kb on - strand, within LRK53_RS03280 at 774.501 kb on - strand, within LRK53_RS03280 at 774.501 kb on - strand, within LRK53_RS03280 at 774.560 kb on + strand at 774.560 kb on + strand at 774.560 kb on + strand at 774.569 kb on + strand at 774.569 kb on + strand at 774.570 kb on - strand at 774.570 kb on - strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.623 kb on + strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.624 kb on - strand at 774.726 kb on + strand at 774.772 kb on - strand at 774.971 kb on + strand at 774.972 kb on - strand at 775.076 kb on + strand, within LRK53_RS03285 at 775.077 kb on - strand, within LRK53_RS03285 at 775.193 kb on + strand, within LRK53_RS03285 at 775.193 kb on + strand, within LRK53_RS03285 at 775.194 kb on - strand, within LRK53_RS03285 at 775.194 kb on - strand, within LRK53_RS03285 at 775.334 kb on + strand, within LRK53_RS03285 at 775.334 kb on + strand, within LRK53_RS03285 at 775.335 kb on - strand, within LRK53_RS03285 at 775.346 kb on + strand, within LRK53_RS03285 at 775.346 kb on + strand, within LRK53_RS03285 at 775.466 kb on + strand, within LRK53_RS03285 at 775.466 kb on + strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.467 kb on - strand, within LRK53_RS03285 at 775.493 kb on + strand, within LRK53_RS03285 at 775.493 kb on + strand, within LRK53_RS03285 at 775.494 kb on - strand, within LRK53_RS03285 at 775.494 kb on - strand, within LRK53_RS03285 at 775.494 kb on - strand, within LRK53_RS03285 at 775.494 kb on - strand, within LRK53_RS03285 at 775.505 kb on + strand, within LRK53_RS03285 at 775.505 kb on + strand, within LRK53_RS03285 at 775.505 kb on + strand, within LRK53_RS03285 at 775.505 kb on + strand, within LRK53_RS03285 at 775.505 kb on + strand, within LRK53_RS03285 at 775.505 kb on + strand, within LRK53_RS03285 at 775.506 kb on - strand, within LRK53_RS03285 at 775.506 kb on - strand, within LRK53_RS03285 at 775.506 kb on - strand, within LRK53_RS03285 at 775.506 kb on - strand, within LRK53_RS03285 at 775.506 kb on - strand, within LRK53_RS03285 at 775.506 kb on - strand, within LRK53_RS03285 at 775.506 kb on - strand, within LRK53_RS03285 at 775.550 kb on + strand, within LRK53_RS03285 at 775.550 kb on + strand, within LRK53_RS03285 at 775.550 kb on + strand, within LRK53_RS03285 at 775.551 kb on - strand, within LRK53_RS03285 at 775.551 kb on - strand, within LRK53_RS03285 at 775.551 kb on - strand, within LRK53_RS03285 at 775.551 kb on - strand, within LRK53_RS03285 at 775.551 kb on - strand, within LRK53_RS03285 at 775.551 kb on - strand, within LRK53_RS03285 at 775.580 kb on + strand, within LRK53_RS03285 at 775.580 kb on + strand, within LRK53_RS03285 at 775.581 kb on - strand, within LRK53_RS03285 at 775.581 kb on - strand, within LRK53_RS03285 at 775.581 kb on - strand, within LRK53_RS03285 at 775.581 kb on - strand, within LRK53_RS03285 at 775.581 kb on - strand, within LRK53_RS03285 at 775.581 kb on - strand, within LRK53_RS03285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 772,708 + LRK53_RS03275 0.45 -2.1 772,708 + LRK53_RS03275 0.45 -1.9 772,708 + LRK53_RS03275 0.45 +1.7 772,708 + LRK53_RS03275 0.45 +0.7 772,708 + LRK53_RS03275 0.45 -0.9 772,708 + LRK53_RS03275 0.45 -0.8 772,709 - LRK53_RS03275 0.46 -0.5 772,709 - LRK53_RS03275 0.46 +1.1 772,709 - LRK53_RS03275 0.46 +0.5 772,709 - LRK53_RS03275 0.46 -1.7 772,837 + LRK53_RS03275 0.54 +0.5 772,933 + LRK53_RS03275 0.60 -0.9 772,933 + LRK53_RS03275 0.60 -1.9 772,933 + LRK53_RS03275 0.60 -0.9 772,934 - LRK53_RS03275 0.60 +1.1 772,934 - LRK53_RS03275 0.60 +2.1 772,934 - LRK53_RS03275 0.60 +0.1 772,934 - LRK53_RS03275 0.60 -3.2 772,972 + LRK53_RS03275 0.63 +0.8 772,973 - LRK53_RS03275 0.63 +0.8 772,973 - LRK53_RS03275 0.63 -0.7 772,973 - LRK53_RS03275 0.63 +0.1 773,416 + +0.1 773,437 + -0.1 773,438 - +1.1 773,479 + -0.9 773,479 + -2.1 773,479 + +0.4 773,479 + -0.9 773,479 + -0.9 773,479 + -0.0 773,479 + -0.8 773,480 - -0.7 773,480 - -0.9 773,480 - +0.5 773,480 - +1.1 773,480 - -0.9 773,480 - +1.4 773,480 - -1.3 773,489 - -2.4 773,489 - -2.2 773,885 + LRK53_RS03280 0.31 -0.9 774,131 + LRK53_RS03280 0.54 -0.9 774,131 + LRK53_RS03280 0.54 -0.9 774,131 + LRK53_RS03280 0.54 +1.1 774,131 + LRK53_RS03280 0.54 -0.9 774,131 + LRK53_RS03280 0.54 -0.7 774,131 + LRK53_RS03280 0.54 -0.3 774,131 + LRK53_RS03280 0.54 -1.9 774,131 + LRK53_RS03280 0.54 -1.5 774,131 + LRK53_RS03280 0.54 +2.1 774,131 + LRK53_RS03280 0.54 +1.1 774,131 + LRK53_RS03280 0.54 -0.2 774,131 + LRK53_RS03280 0.54 +0.5 774,132 - LRK53_RS03280 0.54 -1.6 774,132 - LRK53_RS03280 0.54 -1.1 774,132 - LRK53_RS03280 0.54 -0.5 774,132 - LRK53_RS03280 0.54 +0.5 774,132 - LRK53_RS03280 0.54 -0.5 774,132 - LRK53_RS03280 0.54 -1.1 774,132 - LRK53_RS03280 0.54 -2.1 774,132 - LRK53_RS03280 0.54 -1.7 774,132 - LRK53_RS03280 0.54 -1.2 774,132 - LRK53_RS03280 0.54 +1.1 774,132 - LRK53_RS03280 0.54 -0.5 774,132 - LRK53_RS03280 0.54 -0.3 774,140 + LRK53_RS03280 0.55 +0.1 774,140 + LRK53_RS03280 0.55 +0.1 774,140 + LRK53_RS03280 0.55 -1.7 774,141 - LRK53_RS03280 0.55 -0.9 774,149 - LRK53_RS03280 0.56 +0.1 774,149 - LRK53_RS03280 0.56 +0.7 774,149 - LRK53_RS03280 0.56 +1.1 774,149 - LRK53_RS03280 0.56 +0.8 774,501 - LRK53_RS03280 0.88 +0.4 774,501 - LRK53_RS03280 0.88 -0.7 774,501 - LRK53_RS03280 0.88 -0.5 774,501 - LRK53_RS03280 0.88 -0.9 774,501 - LRK53_RS03280 0.88 +0.1 774,501 - LRK53_RS03280 0.88 -1.7 774,560 + -2.1 774,560 + +0.1 774,560 + -1.5 774,569 + -1.7 774,569 + -1.5 774,570 - -1.0 774,570 - -0.9 774,623 + -0.9 774,623 + -0.5 774,623 + -0.8 774,623 + +0.5 774,623 + +1.1 774,623 + +0.1 774,623 + +1.1 774,623 + -1.0 774,623 + -1.1 774,623 + +0.1 774,623 + +0.1 774,623 + -0.9 774,623 + -0.6 774,624 - +1.1 774,624 - -1.1 774,624 - -1.5 774,624 - -0.2 774,624 - +0.1 774,624 - -1.3 774,624 - +0.1 774,624 - -1.5 774,624 - -2.4 774,624 - +0.1 774,624 - -0.3 774,624 - -0.5 774,726 + -1.5 774,772 - -0.3 774,971 + -1.1 774,972 - -1.6 775,076 + LRK53_RS03285 0.11 -0.3 775,077 - LRK53_RS03285 0.11 +0.1 775,193 + LRK53_RS03285 0.17 -0.5 775,193 + LRK53_RS03285 0.17 +1.3 775,194 - LRK53_RS03285 0.17 -0.1 775,194 - LRK53_RS03285 0.17 +1.1 775,334 + LRK53_RS03285 0.24 -1.1 775,334 + LRK53_RS03285 0.24 +0.5 775,335 - LRK53_RS03285 0.24 -1.5 775,346 + LRK53_RS03285 0.25 -1.2 775,346 + LRK53_RS03285 0.25 +1.1 775,466 + LRK53_RS03285 0.31 +0.1 775,466 + LRK53_RS03285 0.31 -0.8 775,467 - LRK53_RS03285 0.31 -1.5 775,467 - LRK53_RS03285 0.31 +2.1 775,467 - LRK53_RS03285 0.31 -0.4 775,467 - LRK53_RS03285 0.31 +0.1 775,467 - LRK53_RS03285 0.31 -1.5 775,467 - LRK53_RS03285 0.31 +0.5 775,467 - LRK53_RS03285 0.31 -1.9 775,467 - LRK53_RS03285 0.31 +0.1 775,467 - LRK53_RS03285 0.31 -0.4 775,493 + LRK53_RS03285 0.33 -0.9 775,493 + LRK53_RS03285 0.33 +0.7 775,494 - LRK53_RS03285 0.33 -0.9 775,494 - LRK53_RS03285 0.33 +0.1 775,494 - LRK53_RS03285 0.33 +2.1 775,494 - LRK53_RS03285 0.33 +0.7 775,505 + LRK53_RS03285 0.33 -0.9 775,505 + LRK53_RS03285 0.33 -2.6 775,505 + LRK53_RS03285 0.33 -0.5 775,505 + LRK53_RS03285 0.33 -0.5 775,505 + LRK53_RS03285 0.33 -0.6 775,505 + LRK53_RS03285 0.33 -0.6 775,506 - LRK53_RS03285 0.33 +1.1 775,506 - LRK53_RS03285 0.33 -1.2 775,506 - LRK53_RS03285 0.33 -1.2 775,506 - LRK53_RS03285 0.33 -0.5 775,506 - LRK53_RS03285 0.33 +0.5 775,506 - LRK53_RS03285 0.33 -0.5 775,506 - LRK53_RS03285 0.33 -0.1 775,550 + LRK53_RS03285 0.36 -0.5 775,550 + LRK53_RS03285 0.36 -0.9 775,550 + LRK53_RS03285 0.36 -0.7 775,551 - LRK53_RS03285 0.36 -0.5 775,551 - LRK53_RS03285 0.36 -1.1 775,551 - LRK53_RS03285 0.36 +1.5 775,551 - LRK53_RS03285 0.36 +0.7 775,551 - LRK53_RS03285 0.36 -2.0 775,551 - LRK53_RS03285 0.36 +0.5 775,580 + LRK53_RS03285 0.37 +0.1 775,580 + LRK53_RS03285 0.37 -0.5 775,581 - LRK53_RS03285 0.37 +0.1 775,581 - LRK53_RS03285 0.37 -0.9 775,581 - LRK53_RS03285 0.37 -1.6 775,581 - LRK53_RS03285 0.37 -0.2 775,581 - LRK53_RS03285 0.37 +0.1 775,581 - LRK53_RS03285 0.37 +2.1
Or see this region's nucleotide sequence