Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS02400 and LRK53_RS02405 are separated by 5 nucleotides LRK53_RS02405 and LRK53_RS02410 are separated by 178 nucleotides LRK53_RS02410 and LRK53_RS02415 are separated by 247 nucleotides
LRK53_RS02400: LRK53_RS02400 - exodeoxyribonuclease III, at 550,810 to 551,577
_RS02400
LRK53_RS02405: LRK53_RS02405 - YecA family protein, at 551,583 to 552,260
_RS02405
LRK53_RS02410: LRK53_RS02410 - pirin family protein, at 552,439 to 553,272
_RS02410
LRK53_RS02415: LRK53_RS02415 - carbohydrate ABC transporter permease, at 553,520 to 554,293
_RS02415
Position (kb) 552
553
554 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.571 kb on + strand at 551.572 kb on - strand at 551.572 kb on - strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.646 kb on + strand at 551.647 kb on - strand at 551.863 kb on - strand, within LRK53_RS02405 at 552.090 kb on + strand, within LRK53_RS02405 at 552.091 kb on - strand, within LRK53_RS02405 at 552.091 kb on - strand, within LRK53_RS02405 at 552.091 kb on - strand, within LRK53_RS02405 at 552.141 kb on + strand, within LRK53_RS02405 at 552.141 kb on + strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.142 kb on - strand, within LRK53_RS02405 at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.280 kb on + strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.281 kb on - strand at 552.386 kb on + strand at 552.386 kb on + strand at 552.387 kb on - strand at 552.387 kb on - strand at 552.387 kb on - strand at 552.387 kb on - strand at 552.592 kb on + strand, within LRK53_RS02410 at 552.592 kb on + strand, within LRK53_RS02410 at 552.592 kb on + strand, within LRK53_RS02410 at 552.592 kb on + strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.593 kb on - strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.643 kb on + strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.644 kb on - strand, within LRK53_RS02410 at 552.646 kb on - strand, within LRK53_RS02410 at 552.838 kb on + strand, within LRK53_RS02410 at 552.964 kb on + strand, within LRK53_RS02410 at 552.964 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.997 kb on + strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 552.998 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.037 kb on - strand, within LRK53_RS02410 at 553.270 kb on + strand at 553.270 kb on + strand at 553.271 kb on - strand at 553.271 kb on - strand at 553.271 kb on - strand at 553.271 kb on - strand at 553.271 kb on - strand at 553.271 kb on - strand at 553.271 kb on - strand at 553.271 kb on - strand at 553.273 kb on + strand at 553.273 kb on + strand at 553.273 kb on + strand at 553.273 kb on + strand at 553.273 kb on + strand at 553.274 kb on - strand at 553.274 kb on - strand at 553.274 kb on - strand at 553.274 kb on - strand at 553.274 kb on - strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.383 kb on + strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.384 kb on - strand at 553.385 kb on - strand at 553.557 kb on + strand at 553.558 kb on - strand at 553.558 kb on - strand at 553.558 kb on - strand at 553.558 kb on - strand at 553.683 kb on + strand, within LRK53_RS02415 at 553.683 kb on + strand, within LRK53_RS02415 at 553.683 kb on + strand, within LRK53_RS02415 at 553.684 kb on - strand, within LRK53_RS02415 at 553.684 kb on - strand, within LRK53_RS02415 at 553.684 kb on - strand, within LRK53_RS02415 at 553.684 kb on - strand, within LRK53_RS02415 at 553.684 kb on - strand, within LRK53_RS02415 at 553.684 kb on - strand, within LRK53_RS02415 at 553.684 kb on - strand, within LRK53_RS02415 at 553.872 kb on + strand, within LRK53_RS02415 at 553.873 kb on - strand, within LRK53_RS02415 at 553.873 kb on - strand, within LRK53_RS02415 at 553.873 kb on - strand, within LRK53_RS02415 at 553.932 kb on + strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.933 kb on - strand, within LRK53_RS02415 at 553.953 kb on + strand, within LRK53_RS02415 at 553.953 kb on + strand, within LRK53_RS02415 at 553.953 kb on + strand, within LRK53_RS02415 at 553.953 kb on + strand, within LRK53_RS02415 at 553.953 kb on + strand, within LRK53_RS02415 at 553.954 kb on - strand, within LRK53_RS02415 at 553.954 kb on - strand, within LRK53_RS02415 at 553.954 kb on - strand, within LRK53_RS02415 at 553.954 kb on - strand, within LRK53_RS02415 at 553.954 kb on - strand, within LRK53_RS02415 at 553.954 kb on - strand, within LRK53_RS02415 at 553.954 kb on - strand, within LRK53_RS02415 at 554.067 kb on + strand, within LRK53_RS02415 at 554.131 kb on + strand, within LRK53_RS02415 at 554.132 kb on - strand, within LRK53_RS02415 at 554.139 kb on + strand, within LRK53_RS02415 at 554.176 kb on - strand, within LRK53_RS02415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 551,571 + -0.5 551,571 + -1.5 551,571 + -0.5 551,571 + -0.2 551,571 + +1.1 551,571 + -2.4 551,571 + +0.1 551,571 + -0.6 551,572 - +2.1 551,572 - -0.9 551,646 + -0.5 551,646 + -1.2 551,646 + -0.6 551,646 + -1.2 551,646 + +0.1 551,647 - -0.5 551,863 - LRK53_RS02405 0.41 -0.5 552,090 + LRK53_RS02405 0.75 -0.5 552,091 - LRK53_RS02405 0.75 +0.1 552,091 - LRK53_RS02405 0.75 -3.3 552,091 - LRK53_RS02405 0.75 +1.1 552,141 + LRK53_RS02405 0.82 -0.5 552,141 + LRK53_RS02405 0.82 -1.2 552,142 - LRK53_RS02405 0.82 +1.1 552,142 - LRK53_RS02405 0.82 -0.5 552,142 - LRK53_RS02405 0.82 -0.2 552,142 - LRK53_RS02405 0.82 +0.4 552,280 + -1.2 552,280 + -0.5 552,280 + -1.5 552,280 + +0.7 552,280 + -1.2 552,280 + -0.3 552,280 + -0.9 552,280 + -0.5 552,280 + +2.1 552,281 - +0.6 552,281 - +0.1 552,281 - -2.2 552,281 - -1.2 552,281 - -1.5 552,281 - -0.5 552,281 - +1.1 552,281 - +1.1 552,281 - -0.7 552,281 - -1.3 552,386 + -1.2 552,386 + +0.7 552,387 - -0.9 552,387 - +0.4 552,387 - -1.5 552,387 - +0.4 552,592 + LRK53_RS02410 0.18 -0.7 552,592 + LRK53_RS02410 0.18 -1.3 552,592 + LRK53_RS02410 0.18 -1.5 552,592 + LRK53_RS02410 0.18 +1.1 552,593 - LRK53_RS02410 0.18 -0.5 552,593 - LRK53_RS02410 0.18 -1.1 552,593 - LRK53_RS02410 0.18 -0.5 552,593 - LRK53_RS02410 0.18 -1.1 552,593 - LRK53_RS02410 0.18 -0.9 552,593 - LRK53_RS02410 0.18 +0.1 552,593 - LRK53_RS02410 0.18 -0.5 552,593 - LRK53_RS02410 0.18 -1.2 552,643 + LRK53_RS02410 0.24 +0.1 552,643 + LRK53_RS02410 0.24 -1.5 552,643 + LRK53_RS02410 0.24 -0.2 552,643 + LRK53_RS02410 0.24 -0.7 552,643 + LRK53_RS02410 0.24 -1.2 552,643 + LRK53_RS02410 0.24 -0.5 552,643 + LRK53_RS02410 0.24 +0.1 552,643 + LRK53_RS02410 0.24 -2.0 552,643 + LRK53_RS02410 0.24 -0.4 552,643 + LRK53_RS02410 0.24 -0.8 552,643 + LRK53_RS02410 0.24 -1.2 552,643 + LRK53_RS02410 0.24 -2.2 552,643 + LRK53_RS02410 0.24 +1.1 552,643 + LRK53_RS02410 0.24 -1.5 552,643 + LRK53_RS02410 0.24 +0.5 552,643 + LRK53_RS02410 0.24 -0.3 552,643 + LRK53_RS02410 0.24 +0.5 552,643 + LRK53_RS02410 0.24 -1.4 552,643 + LRK53_RS02410 0.24 +0.1 552,643 + LRK53_RS02410 0.24 -0.5 552,643 + LRK53_RS02410 0.24 +1.1 552,643 + LRK53_RS02410 0.24 -1.5 552,643 + LRK53_RS02410 0.24 -2.6 552,643 + LRK53_RS02410 0.24 +0.7 552,643 + LRK53_RS02410 0.24 +0.1 552,643 + LRK53_RS02410 0.24 -0.1 552,643 + LRK53_RS02410 0.24 -0.5 552,643 + LRK53_RS02410 0.24 -0.2 552,643 + LRK53_RS02410 0.24 +1.1 552,643 + LRK53_RS02410 0.24 -0.5 552,643 + LRK53_RS02410 0.24 -0.5 552,643 + LRK53_RS02410 0.24 -1.6 552,643 + LRK53_RS02410 0.24 -1.7 552,644 - LRK53_RS02410 0.25 -0.5 552,644 - LRK53_RS02410 0.25 -0.9 552,644 - LRK53_RS02410 0.25 -0.2 552,644 - LRK53_RS02410 0.25 -1.1 552,644 - LRK53_RS02410 0.25 -0.9 552,644 - LRK53_RS02410 0.25 -0.2 552,644 - LRK53_RS02410 0.25 +0.5 552,644 - LRK53_RS02410 0.25 -1.7 552,644 - LRK53_RS02410 0.25 -1.1 552,644 - LRK53_RS02410 0.25 -1.2 552,644 - LRK53_RS02410 0.25 -0.3 552,644 - LRK53_RS02410 0.25 -1.5 552,644 - LRK53_RS02410 0.25 -0.9 552,644 - LRK53_RS02410 0.25 -0.5 552,644 - LRK53_RS02410 0.25 -0.4 552,644 - LRK53_RS02410 0.25 -0.7 552,644 - LRK53_RS02410 0.25 -0.5 552,644 - LRK53_RS02410 0.25 +0.3 552,644 - LRK53_RS02410 0.25 -1.9 552,644 - LRK53_RS02410 0.25 -2.0 552,644 - LRK53_RS02410 0.25 -0.7 552,644 - LRK53_RS02410 0.25 -0.7 552,644 - LRK53_RS02410 0.25 +0.1 552,644 - LRK53_RS02410 0.25 -1.6 552,646 - LRK53_RS02410 0.25 +0.1 552,838 + LRK53_RS02410 0.48 +0.1 552,964 + LRK53_RS02410 0.63 +0.1 552,964 + LRK53_RS02410 0.63 +2.1 552,997 + LRK53_RS02410 0.67 -1.2 552,997 + LRK53_RS02410 0.67 -0.5 552,997 + LRK53_RS02410 0.67 +0.7 552,997 + LRK53_RS02410 0.67 -0.8 552,997 + LRK53_RS02410 0.67 -3.9 552,997 + LRK53_RS02410 0.67 -1.7 552,997 + LRK53_RS02410 0.67 -1.5 552,997 + LRK53_RS02410 0.67 +0.4 552,998 - LRK53_RS02410 0.67 +0.1 552,998 - LRK53_RS02410 0.67 -0.3 552,998 - LRK53_RS02410 0.67 -1.4 552,998 - LRK53_RS02410 0.67 -1.2 552,998 - LRK53_RS02410 0.67 -1.2 553,037 - LRK53_RS02410 0.72 -0.1 553,037 - LRK53_RS02410 0.72 +1.1 553,037 - LRK53_RS02410 0.72 +0.1 553,037 - LRK53_RS02410 0.72 -0.7 553,037 - LRK53_RS02410 0.72 -0.2 553,037 - LRK53_RS02410 0.72 -1.7 553,270 + +0.1 553,270 + +0.7 553,271 - -0.7 553,271 - +0.5 553,271 - -0.5 553,271 - +0.1 553,271 - +0.2 553,271 - -1.5 553,271 - +0.8 553,271 - -1.1 553,273 + -0.5 553,273 + +0.3 553,273 + -0.6 553,273 + -0.5 553,273 + -1.2 553,274 - +0.1 553,274 - -0.2 553,274 - -0.5 553,274 - +0.1 553,274 - -0.9 553,383 + -1.2 553,383 + -0.7 553,383 + -0.9 553,383 + +0.1 553,383 + +1.1 553,383 + -1.2 553,383 + -0.9 553,383 + -0.6 553,383 + -1.2 553,383 + -0.6 553,383 + -0.9 553,383 + -0.4 553,383 + -1.2 553,383 + +2.7 553,383 + -0.5 553,383 + -0.5 553,383 + +0.1 553,383 + -1.4 553,383 + +1.1 553,383 + -1.7 553,383 + -0.3 553,383 + +1.7 553,383 + +0.2 553,383 + -0.9 553,383 + -0.6 553,383 + -0.9 553,383 + -1.9 553,383 + -1.2 553,383 + -0.9 553,384 - -0.7 553,384 - +1.7 553,384 - -1.3 553,384 - +0.1 553,384 - -1.2 553,384 - -0.0 553,384 - -0.9 553,384 - -0.9 553,384 - +0.5 553,384 - -0.9 553,384 - -0.7 553,384 - -1.2 553,384 - +1.1 553,384 - +0.1 553,384 - +0.9 553,384 - -0.2 553,384 - -0.7 553,384 - -2.0 553,384 - +1.1 553,384 - +0.1 553,384 - -0.9 553,384 - -0.1 553,384 - -0.5 553,384 - -0.9 553,384 - -0.7 553,384 - -1.1 553,384 - +0.1 553,384 - +0.9 553,385 - -0.9 553,557 + +0.1 553,558 - +2.7 553,558 - -2.0 553,558 - -1.7 553,558 - +0.1 553,683 + LRK53_RS02415 0.21 -1.9 553,683 + LRK53_RS02415 0.21 +0.8 553,683 + LRK53_RS02415 0.21 -0.8 553,684 - LRK53_RS02415 0.21 -1.7 553,684 - LRK53_RS02415 0.21 -0.1 553,684 - LRK53_RS02415 0.21 +0.1 553,684 - LRK53_RS02415 0.21 -0.8 553,684 - LRK53_RS02415 0.21 -0.2 553,684 - LRK53_RS02415 0.21 -0.5 553,684 - LRK53_RS02415 0.21 -1.7 553,872 + LRK53_RS02415 0.45 -0.7 553,873 - LRK53_RS02415 0.46 -1.2 553,873 - LRK53_RS02415 0.46 -0.9 553,873 - LRK53_RS02415 0.46 -1.2 553,932 + LRK53_RS02415 0.53 -1.9 553,933 - LRK53_RS02415 0.53 +0.1 553,933 - LRK53_RS02415 0.53 +1.1 553,933 - LRK53_RS02415 0.53 -2.1 553,933 - LRK53_RS02415 0.53 -0.1 553,933 - LRK53_RS02415 0.53 +0.1 553,933 - LRK53_RS02415 0.53 +1.6 553,933 - LRK53_RS02415 0.53 -1.9 553,933 - LRK53_RS02415 0.53 -0.2 553,933 - LRK53_RS02415 0.53 +0.1 553,953 + LRK53_RS02415 0.56 -0.1 553,953 + LRK53_RS02415 0.56 +0.1 553,953 + LRK53_RS02415 0.56 +0.1 553,953 + LRK53_RS02415 0.56 +2.1 553,953 + LRK53_RS02415 0.56 +2.1 553,954 - LRK53_RS02415 0.56 +0.1 553,954 - LRK53_RS02415 0.56 +0.1 553,954 - LRK53_RS02415 0.56 +0.1 553,954 - LRK53_RS02415 0.56 -0.9 553,954 - LRK53_RS02415 0.56 -1.5 553,954 - LRK53_RS02415 0.56 -1.2 553,954 - LRK53_RS02415 0.56 +1.1 554,067 + LRK53_RS02415 0.71 -1.9 554,131 + LRK53_RS02415 0.79 +0.5 554,132 - LRK53_RS02415 0.79 +1.1 554,139 + LRK53_RS02415 0.80 -1.5 554,176 - LRK53_RS02415 0.85 +0.1
Or see this region's nucleotide sequence